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Int J Mol Sci ; 21(9)2020 May 07.
Artículo en Inglés | MEDLINE | ID: mdl-32392868

RESUMEN

2,4-Dichlorophenol (2,4-DCP) is a ubiquitous environmental pollutant categorized as a priority pollutant by the United States (US) Environmental Protection Agency, posing adverse health effects on humans and wildlife. Bioremediation is proposed as an eco-friendly, cost-effective alternative to traditional physicochemical remediation techniques. In the present study, fungal strains were isolated from marine invertebrates and tested for their ability to biotransform 2,4-DCP at a concentration of 1 mM. The most competent strains were studied further for the expression of catechol dioxygenase activities and the produced metabolites. One strain, identified as Tritirachium sp., expressed high levels of extracellular catechol 1,2-dioxygenase activity. The same strain also produced a dechlorinated cleavage product of the starting compound, indicating the assimilation of the xenobiotic by the fungus. This work also enriches the knowledge about the mechanisms employed by marine-derived fungi in order to defend themselves against chlorinated xenobiotics.


Asunto(s)
Basidiomycota/fisiología , Clorofenoles/metabolismo , Invertebrados/microbiología , Animales , Organismos Acuáticos/microbiología , Basidiomycota/enzimología , Basidiomycota/aislamiento & purificación , Biodegradación Ambiental , Catecol 1,2-Dioxigenasa/metabolismo , Proteínas Fúngicas/metabolismo , Humanos , Simbiosis , Contaminantes Químicos del Agua/metabolismo
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