Cultivation of yeasts on liquid digestate to remove organic pollutants and nutrients and for potential application as co-culture with microalgae.

In this study, the growth of yeast and yeast-like fungi in the liquid digestate from vegetable wastes was investigated in order to remove nutrients and organic pollutants, and for their application as co-culture members with green microalgae. The studied yeast strains were characterized for their assimilative and enzymatic profiles as well as temperature requirements. In the first experimental stage, the growth dynamics of each strain were determined, allowing to select the best yeasts for further studies. In the subsequent stage, the ability of selectants to remove organic pollutants was assessed. Different cultivation media containing respectively 1:3, 1:1, 3:1 vol ratio of liquid digestate and the basal minimal medium were used. Among all tested yeast strains, Rhodotorula mucilaginosa DSM 70825 showed the most promising results, demonstrating the highest potential for removing organic substrates and nutrients. Depending on the medium, this strain achieved 50-80% sCOD, 45-60% tVFAs, 21-45% TN, 33-52% PO43- reduction rates. Similar results were obtained for the strain Candida sp. OR687571. The high nutrient and organics removal efficiency by these yeasts could likely be linked to their ability to assimilate xylose (being the main source of carbon in the liquid digestate). In culture media containing liquid digestate, both yeast strains achieved good viability and proliferation potential. In the liquid digestate medium, R. mucilaginosa and Candida sp. showed vitality at the level of 51.5% and 45.0%, respectively. These strains seem to be a good starting material for developing effective digestate treatment strategies involving monocultures and/or consortia with other yeasts or green microalgae.

Effect of photocatalysis on the physicochemical properties of liquid digestate.

Antibiotic residues pose a risk to the agricultural application of liquid digestate. In our previous study, photocatalysis was employed to degrade the antibiotics in liquid digestate and observed that the removal efficiency of TC, OTC, and CTC was up to 94.99%, 88.92%, and 95.52%, respectively, at the optimal experimental level, demonstrating the feasibility of this technology. In this study, the liquid digestate after photocatalysis was analyzed to evaluate the effect of photocatalysis on the nutrients, phytotoxicity, and bacterial community of liquid digestate. The results showed that photocatalysis had little effect on the major nutrients TN, TP, and TK in liquid digestate. However, photocatalysis could cause an increase in tryptophan substances as well as soluble microbial by-products and a decrease in humic acid substances in the liquid digestate. The toxicity of liquid digestate after photocatalysis exhibited an increasing trend followed by a decreasing trend, and the liquid digestate after photocatalysis for 2 h had a promoting effect on seed germination and root growth. The richness, diversity, and evenness of bacterial communities in liquid digestate were decreased as a result of photocatalysis. The dominant species in the liquid digestate was dramatically changed by photocatalysis, and the antibiotic concentration also had a major effect on the dominant species in the liquid digestate after photocatalysis. After photocatalysis for 2 h, the dominant species in the liquid digestate changed from Firmicutes to Proteobacteria.

Effect of solar and artificial lighting on microalgae cultivation and treatment of liquid digestate.

A comparative study was carried out to assess the effect of two light sources on microalgae cultivation and the treatment of liquid digestate. The R1 photobioreactor operated with LED lightning allowed to achieve moderate nutrient removal rates whereas soluble COD (Chemical Oxygen Demand) was reduced in 90%. After switching this reactor into sunlight, the removal rate of phosphates increased to 66%. However, the greatest removal rate of both nutrients and sCOD of up to 93% was observed in the R2 photobioreactor operated only under sunlight. Microglena sp. was the dominant algae growing in the R1 reactor, and the main bacteria families detected were Chitinophagaceae, Sphingomonadaceae and Xanthobacteraceae. In contrast, Tetradesmus obliquus dominated in the R2 reactor and Rhodanobacteraceae, Chitinophagaceae and A4b were predominant bacteria in this run. Furthermore, much greater biomass productivity as well as overall biomass density was observed in the R2 photobioreactor cultivated exclusively with solar lightning.

Towards biomass production and wastewater treatment by enhancing the microalgae-based nutrients recovery from liquid digestate in an innovative photobioreactor integrated with dialysis bag.

Microalgae-based nutrients recovery from liquid anaerobic digestate of swine manure has been a hotspot in recent decades. Nevertheless, in consideration of the high NH4+-N content and poor light penetrability exhibited by the original liquid digestate, uneconomical pretreatment on liquid digestate including centrifugation and dilution are indispensable before microalgae cells inoculation. Herein, aiming at eliminating the energy-intensive and freshwater-consuming pretreatment on liquid digestate and enhancing microalgae growth, the dialysis bag which permits nutrients transferring across its wall surface whereas retains almost all matters characterized by impeding light transmission within the raw liquid digestate was integrated into a column photobioreactor (DB-PBR). Consequently, light availability of microalgae cells in DB-PBR was elevated remarkably and thus contributed to a 357.58% improvement on microalgae biomass concentration in DB-PBR than the conventional PBR under 80 µmol m-2 s-1. Likewise, superior nutrients removal efficiencies from liquid digestate were obtained in DB-PBR (NH4+-N: 74.84%, TP: 63.75%) over the conventional PBR (NH4+-N: 30.27%, TP: 16.86%). Furthermore, higher microalgae biomass concentration (1.87 g L-1) and nutrients removal efficiencies (NH4+-N: 95.12%, TP: 76.87%) were achieved in the DB-PBR by increasing the light intensity to 140 µmol m-2 s-1. More importantly, the DB-PBR may provide a simple and greener solution to purify other kinds of wastewater.

Treatment of Liquid Digestate by Green Algal Isolates from Artificial Eutrophic Pond.

The ability of aquatic microalgae to treat the liquid digestate obtained from the anaerobic digestion of plant waste was investigated. Microalgae were isolated from natural environment for a laboratory-scale cultivation and were then used to remove nutrients and organic contaminants from the liquid digestate. It was shown that the microalgae consortia (Tetradesmus obliquus, Microglena sp., Desmodesmus subspicatus) could reduce nitrogen, phosphates, and total COD by up to 70%, 57%, and 95%, respectively. A new algae genus Microglena was isolated, which in a consortium with Tetradesmus obliquus and Desmodesmus subspicatus exhibited a high efficiency in the removal of both organic contaminants and nutrients from the liquid fraction of digestate.

A review of advances in valorization and post-treatment of anaerobic digestion liquid fraction effluent.

Traditionally, digestate is considered a waste, which is used as fertiliser in the agriculture industry. Recent studies focus on increasing the profitability of digestate by extracting reusable nutrients to promote biogas plants cost-effectiveness, sustainable management and circular economy. This review focuses on the post-treatment and valorization of liquor which is produced by solid-liquid fractioning of digestate. Nutrient recovery and removal from liquor are possible through mechanical, physicochemical and biological procedures. The processes discussed involve complex procedures that differ in economic value, feasibility, legislative restrictions and performance. The parameters that should be considered to employ these techniques are influenced by liquor characteristics, topography, climate conditions and available resources. These are key parameters to keep in mind during designing and manufacturing a biogas plant. In the following chapters, a discussion on available liquor treatment methods takes place. The present study examines the critical aspects of the available liquor treatment methods.

Investigation on methane yield of wheat husk anaerobic digestion and its enhancement effect by liquid digestate pretreatment.

There are few studies on the use of wheat husk for anaerobic digestion until now. In this study, the BMP (biochemical methane potential) of wheat husk without pretreatment and with liquid digestate (LD) soaking pretreatment was investigated. It was demonstrated that wheat husk could be potentially used as a single feeding substrate for anaerobic digestion with a methane yield of 68 mL/g VS and this yield could be enhanced to a maximum of 130.0 mL/g VS using LD soaking pretreatment, which increased the methane production by 91%. Although the particle size (8, 5, 2 mm) and soaking pretreatment time (1, 3, 5 d) affected the biogas production, the influence of particle size was negligible when the pretreatment time was 5 days. Further analysis of the microbial community showed that the LD soaking pretreatment did not change the predominant microbial communities in the digestion. Therefore, LD pretreatment was effective in enhancing the performance of wheat husk anaerobic digestion.

Semi-continuous anaerobic co-digestion of cow manure and steam-exploded Salix with recirculation of liquid digestate.

The effects of recirculating the liquid fraction of the digestate during mesophilic anaerobic co-digestion of steam-exploded Salix and cow manure were investigated in laboratory-scale continuously stirred tank reactors. An average organic loading rate of 2.6 g VS L(-1) d(-1) and a hydraulic retention time (HRT) of 30 days were employed. Co-digestion of Salix and manure gave better methane yields than digestion of manure alone. Also, a 16% increase in the methane yield was achieved when digestate was recirculated and used instead of water to dilute the feedstock (1:1 dilution ratio). The reactor in which the larger fraction of digestate was recirculated (1:3 dilution ratio) gave the highest methane yields. Ammonia and volatile fatty acids did not reach inhibitory levels, and some potentially inhibitory compounds released during steam explosion (i.e., furfural and 5-hydroxy methyl furfural) were only detected at trace levels throughout the entire study period. However, accumulation of solids, which was more pronounced in the recycling reactors, led to decreased methane yields in those systems after three HRTs. Refraining from the use of fresh water to dilute biomass with a high-solids content and obtaining a final digestate with increased dry matter content might offer important economic benefits in full-scale processes. To ensure long-term stability in such an approach, it would be necessary to optimize separation of the fraction of digestate to be recirculated and also perform proper monitoring to avoid accumulation of solids.

Biogas Slurry Significantly Improved Degraded Farmland Soil Quality and Promoted Capsicum spp. Production.

This study reports on the effects of pretreated biogas slurry on degraded farm soil properties, microflora and the production of Capsicum spp. The responses of soil properties, microorganisms and Capsicum spp. production to biogas slurry pretreated soil were determined. The biogas slurry pretreatment of degraded soil increases the total nitrogen (0.15-0.32 g/kg), total phosphorus (0.13-0.75 g/kg), available phosphorus (102.62-190.68 mg/kg), available potassium (78.94-140.31 mg/kg), organic carbon content (0.67-3.32 g/kg) and pH value of the soil, while the population, diversity and distribution of soil bacteria and fungi were significantly affected. Interestingly, soil ammonium nitrogen, soil pH and soil nitrate nitrogen were highly correlated with the population of bacteria and fungi present in the pretreated soil. The soil with biogas slurry pretreatment of 495 m3/hm2 favored the seedling survival rate, flowering rate and fruit-bearing rate of Capsicum spp. and significantly reduced the rate of rigid seedlings. In this study, the application of 495 m3/hm2 biogas slurry to pretreat degraded soil has achieved the multiple goals of biogas slurry valorization, soil biofertilization and preventing and controlling plant diseases caused by soil-borne pathogenic microorganisms. These findings are of significant importance for the safe and environmentally friendly application of biogas slurry for soil pretreatment.

Biochar-enhanced agricultural application of liquid digestate from food waste anaerobic digestion for celery cultivation.

In this research, the performance of biochar-enhanced agricultural application of food waste liquid digestate for celery cultivation was investigated to reveal its utilization potential and environmental impacts. Liquid digestate demonstrated a good agronomic effect, with a significant fertilization efficiency of 42.3 % during celery growth. With liquid digestate addition (270 t/ha), the same level of harvested celery yield of 15,345 kg/ha was achieved compared with chemical fertilizer utilization of 15,495 kg/ha. Based on the same nitrogen input, the liquid digestate application increased the sugar content of the harvested celery (7 %-15 %) while decreasing the nitrate content (29 %-45 %). The harvested celery with liquid digestate application indicated higher contents of total nitrogen, total phosphorus and total potassium levels than those in the chemical fertilizer group. Liquid digestate as a fertilizer supplemented the soil with nutrients, including phosphorus, potassium and organic matter, but did not cause excessive accumulation. The inorganic nitrogen content of the leachate increased as applied liquid digestate increased. However, it remained 20 %-60 % lower than that of chemical fertilizer at the same fertilization efficiency. After applying liquid digestate, there was no significant increase was observed in soil salinity. The coupled addition of biochar helps to improve the overall effects of liquid digestate for agricultural application and reduce negative environmental impacts. This study demonstrates that returning liquid digestate to agricultural fields as fertilizer is an environmentally and economically beneficial practice.

Effect of temperature and storage methods on liquid digestate: Focusing on the stability, phytotoxicity, and microbial community.

Identifying the stability and phytotoxicity of liquid digestate (LD) is necessary for safe agricultural utilization. Storage temperature, method, and time are critical factors that affect the stability and phytotoxicity of LD. This study therefore aimed to explore the dynamics of stability, phytotoxicity, and microbial community of LD in cattle farms under different storage conditions. The results showed that the contents of solids, organic matter, nitrogen, and phosphorous decreased during storage and exhibited temperature dependency. Conversely, the seed germination index increased, which was negatively correlated with dissolved organic carbon and ammonium nitrogen and positively correlated with certain bacteria (Thermovirga and Fastidiosipila). Open storage and/or higher temperature were found to contribute to the stabilization efficiency and phytotoxicity disappearance of LD. Open storage of LD at 30 °C for 60 days and 20 °C for 90 days was safe for its agricultural utilization, while hermetic storage of LD at 30 °C for 120 days and 20 °C for 150 days was safe. However, for storage at 10 °C for 180 days, additional post-treatment is required.

Developments and Prospects of Farmland Application of Biogas Slurry in China-A Review.

Biogas slurry (BS) is an attractive agricultural waste resource which can be used to regulate soil microbial communities, enhance nutrient absorption capacity of crops, promote plant-soil interactions, and consequently, increase crop productivity. Presently, BS discharge is not environmentally friendly. It is therefore necessary to explore alternative efficient utilization of BS. The use of BS as fertilizer meets the requirements for sustainable and eco-friendly development in agriculture, but this has not been fully actualized. Hence, this paper reviewed the advantages of using BS in farmland as soil fertilization for the improvement of crop production and quality. This review also highlighted the potential of BS for the prevention and control of soil acidification, salinization, as well as improve microbial structure and soil enzyme activity. Moreover, this review reports on the current techniques, application methods, relevant engineering measures, environmental benefits, challenges, and prospects associated with BS utilization. Lastly, additional research efforts require for optimal utilization of BS in farmlands were elucidated.

Valorization of Anaerobic-Fermentation Liquid Digestates-Membrane-Based Process Development.

Complete valorization of various wastes and effluents, with significant organic content, remains a great challenge in the pursuit of a circular economy. The approach based on anaerobic fermentation, leading to valuable biogas production, has been broadly accepted and employed as an attractive processing scheme. However, despite notable research efforts, complete valorization of the digestates (involving recovery of nutrients/by-products and full recycling/reuse of treated water) requires additional work for sustainable process development. This study aims to make a contribution in this direction by demonstrating a systematic methodology for valorizing the liquid digestate. The proposed membrane-based processing scheme involves UF-membrane pretreatment of the liquid digestate (for sludge separation) and subsequent NF/RO membrane treatment for reuse/recycling of the permeate; the concentrate, enriched in "nutrients" (phosphate and ammonium compounds), can be utilized for soil fertilization, with further conditioning/processing. By performing targeted laboratory experiments and advanced simulations, the membrane-based process was developed to a relatively high technology-readiness level, including a pilot unit design/construction and preliminary testing with satisfactory results. Through pilot testing in industrial environment, further process development and optimization will be pursued, towards practical applications. The demonstrated methodology is also considered appropriate for systematic development of membrane-based processes to valorize/treat a variety of similar effluents.

Integrated strategies for robust growth of Chlorella vulgaris on undiluted dairy farm liquid digestate and pollutant removal.

Undiluted dairy farm liquid digestate contains high levels of organic matters, chromaticity and total ammonia nitrogen (TAN), resulting in inhibition to microalgal growth. In this study, a novel cascade pretreatment with ozonation and ammonia stripping (O + S) was employed to remove these inhibitors, and was compared with single pretreatment approach. The optimum parameters for ozonation and ammonia stripping were obtained and the mechanisms of inhibition elimination were investigated. The results show that ozonation contributed to the degradation of non-fluorescent chromophoric organics through the direct molecular ozone attack, which mitigated the inhibition of chromaticity to microalgae, while ammonia stripping relieved the inhibition of high TAN to microalgae. After cascade pretreatment, TAN, total nitrogen (TN), COD and chromaticity were reduced by 80.2 %, 75.4 %, 20.6 % and 75.8 % respectively. When C. vulgaris was cultured on different pretreated digestate, it was found that cascade pretreatment was beneficial for retaining high PSII activity and synergistically improved microalgal growth. The highest biomass increment and productivity achieved 5.40 g L-1 and 900 mg L-1 d-1 respectively in the integration system of cascade pretreatment with microalgae cultivation (O + S + M). After O + S + M treatment, the removal efficiencies of TAN, TN, COD and total phosphorus (TP) were 100 %, 92.8 %, 46.7 % and 99.6 %, respectively. This work provided a promising strategy (O + S + M) for sustainable liquid digestate treatment, along with nutrient recovery and value-added biomass production.

A critical review on retaining antibiotics in liquid digestate: Potential risk and removal technologies.

Substantial levels of antibiotics remain in liquid digestate, posing a significant threat to human safety and the environment. A comprehensive assessment of residual antibiotics in liquid digestate and related removal technologies is required. To this end, this review first evaluates the potential risks of the residual antibiotics in liquid digestate by describing various anaerobic digestion processes and their half-lives in the environment. Next, emerging technologies for removing antibiotics in liquid digestate are summarized and discussed, including membrane separation, adsorption, and advanced oxidation processes. Finally, this study comprehensively and critically discusses these emerging technologies' prospects and challenges, including techno-economic feasibility and environmental impacts.

Treatment and resource utilization of dairy liquid digestate by nitrification of biological aerated filter coupled with assimilation of Chlorella pyrenoidosa.

Ammonia inhibition is considered a key issue when using liquid digestate for microalgae cultivation. To study the effect of pretreatment with a biological aerated filter (BAF) on microalgae culture with dairy liquid digestate, nitrification characteristics of BAFs under different hydraulic retention time (HRT) and the growth characteristics of Chlorella pyrenoidosa in effluents of BAFs were investigated. Results showed that the BAFs can rapidly nitrify ammonia nitrogen and significantly improve the light transmittance of liquid digestate (the maximum promotion rate was ~260%), and the effect improved as the HRT increased. Pretreatment of liquid digestate with BAFs can eliminate ammonia inhibition for C. pyrenoidosa. Furthermore, lipid, crude protein, and higher heating value (HHV) output were also not affected by HRT. The similar removal of nitrate nitrogen in microalgae culture systems using effluents with 6-h and 12-h HRT (21.59% and 21.07%, respectively) were recorded. The results suggested that BAF coupled with microalgae culture is a novel option on the resource utilization of dairy liquid digestate.

Synthesis of nitrogen-enriched hydrochar via co-hydrothermal reaction of liquid digestate and corn stalk.

Synthesis of carbon material from low-cost and sustainable precursors has been intensively explored in recent years. In this study, a nitrogen (N)-enriched hydrochar was developed via a facile one-step hydrothermal carbonization (HTC) of corn stalk (CS) with liquid digestate (LD) of food waste. The LD substituted water and functioned as the N precursor during HTC. The physicochemical properties of hydrochar derived at different HTC temperatures (180-300 °C) were examined and the reaction mechanism was investigated. Intermolecular dehydration and condensation were the primary reactions in the HTC process of CS without LD. The CS-chars maintained the original structure and morphology of the raw corn stalk. The ammonia and inorganic salts in LD promoted the lignin removal, and accelerated the cleavage of the glycosidic linkages of the polysaccharide and hydrogen bonds of cellulose. Benefited from the ammonia and metals in the LD, the recalcitrance structure of the corn stalk was disrupted during the co-HTC even at a low temperature of 220 °C. Moreover, carbon spheres were observed in the LDCS-chars, indicating the LDCS-chars were resulted from sequential hydrolysis, dehydration and condensation during co-HTC reactions. Reactions between N compounds in the LD and derivatives from CS contributed to N doping. The N content of LDCS-chars achieved 4.95% at 260 °C and 83.94% of the N was presented as pyridinic-N. Co-hydrothermal treatment of CS and LD not only enhanced the characteristics of hydrochar, but also recovered two-thirds of ammoniacal N from the digestate to reduce greenhouse gas emission.

Performance evaluation of a solar evaporation system for liquid digestate concentration.

The safe and efficient treatment and resource recovery of the large amount of liquid digestate (LD) produced by biogas plants has become an important factor limiting the development of the biogas industry. In this study, a solar evaporation system was developed based on low cost, high efficiency, and high nutrient recovery of LD concentration. The effects of different parameters, including solar radiation, LD flowrate, and daily treatment capacity, on the concentration efficiency, nutrient recovery, and thermal efficiency were investigated. Economic and enviroeconomic analyses were conducted to comprehensively assess the feasibility and environmental benefits of the system for large-scale biogas plants. The results showed that the higher the solar radiation, the higher the air temperature and its saturation vapor pressure, which led to an increase in the concentration efficiency of LD. The LD concentration efficiency can reach up to 43.33% when the average daily solar radiation was 773.08 W/m2. High solar radiation also contributed to high LD temperature, leading to an increase in the percentage of free ammonia (NH3) in the LD, which was not conducive to the recovery of ammonia nitrogen (NH3N). By increasing the LD flow rate, the heat and mass exchange between air and LD in the spray chamber was enhanced, resulting in higher concentration efficiency. The variation of LD flow rate and daily treatment capacity had little effect on nutrient recovery. The recovery of NH3N and soluble salts in the concentrated phase could be as high as 78.39% and 84.19%, respectively, when the initial pH of LD was 5, the air flow rate was 0.03 m3/s, and the LD flow rate was 6 L/min. In addition, the economic analysis indicated that the concentration cost of the system in treating a daily LD production of 100 tons was 1.08 USD/ton. The solar evaporation system can reduce CO2 emissions by 2.40 × 105 tons and earn carbon credits of 3.48 × 106 USD over its lifetime. This study provides a new direction and reference for LD concentration.

Effects of different pretreatment methods on biogas production and microbial community in anaerobic digestion of wheat straw.

The pretreatment of wheat straw has been recognized to be an essential step prior to anaerobic digestion, owing to the high abundance of lignocellulosic materials. In order to choose economical and effective techniques for the disposal of wheat straw, effects of five pretreatment methods including acid, alkali, co-pretreatment of acid and alkali, CaO2, and liquid digestate of municipal sewage sludge on anaerobic digestion of wheat straw were investigated by analyzing biogas production and organic matter degradation in the study. The results showed that among these pretreatment methods, the methane yield was highest in the liquid digestate pretreated-wheat straw with 112.6 mL gTS-1, followed by the acid, alkali, and CaO2 pretreatments, and the lowest was observed in the co-pretreatment of acid and alkali. Illumina MiSeq sequencing of the microbial communities in the anaerobic digesters revealed that the genera Ruminiclostridium including Ruminiclostridium and Ruminiclostridium 1, Hydrogenispora, and Capriciproducens were the main hydrolytic bacteria, acidogenic bacteria, and acetogenic bacteria, respectively, in the anaerobic digesters. Capriciproducens and Hydrogenispora dominated in the first and the later stages, respectively, in the anaerobic digesters, which could work as indicators of the anaerobic co-digestion stage of sludge and wheat straw. The total solid and SO42--S contents of the solid digestate and the NH4+-N concentration of the liquid digestate had a significant influence on the microbial community in the digesters. These findings indicated that liquid digestate pretreatment was a potential option to improve the anaerobic digestion of wheat straw, due to the low cost without additional chemical agents.

Effects of liquid digestate on the valence state of vanadium in plant and soil and microbial community response.

Liquid digestate containing high levels of nutrients and humic and fatty acids can affect vanadium species and their plant uptake. To elucidate the effects of liquid digestate on the valence state of vanadium in soil and plant tissue, as well as its effects on the microbial community and soil properties, we grew green bristlegrass (Setaria viridis), a native plant capable of growing in vanadium mining areas, in vanadium-contaminated soils sampled from a mining area and treated it with 5% and 10% liquid digestate for 90 d, respectively. Changes in the concentrations of pentavalent (V[V]) and tetravalent (V[IV]) vanadium in the soils and the shoots and roots of bristlegrass and the soil microbial abundance were measured. The results showed that vanadium existed mainly in the form of V(IV) in the soil but accumulated mainly in the form of V(V) in the bristlegrass. Liquid digestate markedly reduced V(V) concentrations in the soils (by up to 45%) and in the shoots and roots of green bristlegrass (by up to 98%). Liquid digestate enhanced the abundance of Bacteroidetes, which can reduce V(V) to lower valence state. Microbial reduction and phosphorus immobilization were responsible for downregulating V(V) concentrations in the plant and soil. The liquid digestate can be used to enhance in situ bioremediation of vanadium-contaminated soil in mining area.