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Relative to a growing body of knowledge about the negative consequences of freshwater salinization, little is known about how aquatic insects respond to progressively ion-poor conditions. Here, we examined life-history and physiological acclimation in Neocloeon triangulifer by rearing nymphs from 1-day post-egg hatch to adulthood across a gradient of decreasing Na concentrations (15, 8, 4, 2 and 1 mg l-1 Na). We found no significant changes in survival, growth, development time and whole-body Na content across these treatments. Radiotracer data revealed that nymphs acclimated to their dilute exposures by increasing their rates of Na uptake and were able to maintain a relatively narrow range of uptake rates (±s.e.m.) of 38.5 ± 4.2 µg Na g-1 h-1 across all treatments. By contrast, the Na uptake rates observed in naive nymphs were much more concentration dependent. This acclimatory response is partially explained by differences in ionocyte counts on the gills of nymphs reared under different salinities. Acclimated nymphs were surprisingly less retentive of their sodium composition when subjected to deionized water challenge. By contrasting our findings with a previous N. triangulifer salinity acclimation study, we show a physiological affinity for dilute conditions in this emerging mayfly model.
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Ephemeroptera , Contaminantes Químicos del Agua , Aclimatación , Animales , Ephemeroptera/fisiología , Agua Dulce , Branquias , Iones , Salinidad , SodioRESUMEN
In recent times, Wireless Sensor Networks (WSNs) are becoming more and more popular and are making significant advances in wireless communication thanks to low-cost and low-power sensors. However, since WSN nodes are battery-powered, they lose all of their autonomy after a certain time. This energy restriction impacts the network's lifetime. Clustering can increase the lifetime of a network while also lowering energy use. Clustering will bring several similar sensors to one location for data collection and delivery to the Base Station (BS). The Cluster Head (CH) uses more energy when collecting and transferring data. The life of the WSNs can be extended, and efficient identification of CH can minimize energy consumption. Creating a routing algorithm that considers the key challenges of lowering energy usage and maximizing network lifetime is still challenging. This paper presents an energy-efficient clustering routing protocol based on a hybrid Mayfly-Aquila optimization (MFA-AOA) algorithm for solving these critical issues in WSNs. The Mayfly algorithm is employed to choose an optimal CH from a collection of nodes. The Aquila optimization algorithm identifies and selects the optimum route between CH and BS. The simulation results showed that the proposed methodology achieved better energy consumption by 10.22%, 11.26%, and 14.28%, and normalized energy by 9.56%, 11.78%, and 13.76% than the existing state-of-art approaches.
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BACKGROUND: The incidence rates of breast cancer in women community is progressively raising and the premature diagnosis is necessary to detect and cure the disease. OBJECTIVE: To develop a novel automated disuse detection framework to examine the Breast-Ultrasound-Images (BUI). METHODS: This scheme includes the following stages; (i) Image acquisition and resizing, (ii) Gaussian filter-based pre-processing, (iii) Handcrafted features extraction, (iv) Optimal feature selection with Mayfly Algorithm (MA), (v) Binary classification and validation. The dataset includes BUI extracted from 133 normal, 445 benign and 210 malignant cases. Each BUI is resized to 256×256×1 pixels and the resized BUIs are used to develop and test the new scheme. Handcrafted feature-based cancer detection is employed and the parameters, such as Entropies, Local-Binary-Pattern (LBP) and Hu moments are considered. To avoid the over-fitting problem, a feature reduction procedure is also implemented with MA and the reduced feature sub-set is used to train and validate the classifiers developed in this research. RESULTS: The experiments were performed to classify BUIs between (i) normal and benign, (ii) normal and malignant, and (iii) benign and malignant cases. The results show that classification accuracy of >â94%, precision of >â92%, sensitivity of >â92% and specificity of >â90% are achieved applying the developed new schemes or framework. CONCLUSION: In this work, a machine-learning scheme is employed to detect/classify the disease using BUI and achieves promising results. In future, we will test the feasibility of implementing deep-learning method to this framework to further improve detection accuracy.
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Neoplasias de la Mama , Ephemeroptera , Algoritmos , Animales , Femenino , Humanos , Ultrasonografía , Ultrasonografía MamariaRESUMEN
Branching morphogenesis helps increase the efficiency of gas and liquid transport in many animal organs. Studies in several model organisms have highlighted the molecular and cellular complexity behind branching morphogenesis. To understand this complexity, computational models have been developed with the goal of identifying the "major rules" that globally explain the branching patterns. These models also guide further experimental exploration of the biological processes that execute and maintain these rules. In this paper we introduce the tracheal gills of mayfly (Ephemeroptera) larvae as a model system to study the generation of branched respiratory patterns. First, we describe the gills of the mayfly Cloeon dipterum, and quantitatively characterize the geometry of its branching trachea. We next extend this characterization to those of related species to generate the morphospace of branching patterns. Then, we show how an algorithm based on the "space colonization" concept (SCA) can generate this branching morphospace via growth towards a hypothetical attractor molecule (M). SCA differs from other branch-generating algorithms in that the geometry generated depends to a great extent on its perception of the "external" space available for branching, uses few rules and, importantly, can be easily translated into a realistic "biological patterning algorithm". We identified a gene in the C. dipterum genome (Cd-bnl) that is orthologous to the fibroblast growth factor branchless (bnl), which stimulates growth and branching of embryonic trachea in Drosophila. In C. dipterum, this gene is expressed in the gill margins and areas of finer tracheolar branching from thicker trachea. Thus, Cd-bnl may perform the function of M in our model. Finally, we discuss this general mechanism in the context of other branching pattern-generating algorithms.
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Tipificación del Cuerpo/genética , Ephemeroptera/embriología , Tráquea/embriología , Algoritmos , Animales , Ephemeroptera/genética , Ephemeroptera/metabolismo , Regulación del Desarrollo de la Expresión Génica/genética , Genes de Insecto/genética , Branquias , Larva/metabolismo , Modelos Biológicos , Morfogénesis , Transducción de Señal , Tráquea/metabolismoRESUMEN
World-Health-Organization (WHO) has listed Tuberculosis (TB) as one among the top 10 reasons for death and an early diagnosis will help to cure the patient by giving suitable treatment. TB usually affects the lungs and an accurate bio-imaging scheme will be apt to diagnose the infection. This research aims to implement an automated scheme to detect TB infection in chest radiographs (X-ray) using a chosen Deep-Learning (DL) approach. The primary objective of the proposed scheme is to attain better classification accuracy while detecting TB in X-ray images. The proposed scheme consists of the following phases namely, (1) image collection and pre-processing, (2) feature extraction with pre-trained VGG16 and VGG19, (3) Mayfly-algorithm (MA) based optimal feature selection, (4) serial feature concatenation and (5) binary classification with a 5-fold cross validation. In this work, the performance of the proposed DL scheme is separately validated for (1) VGG16 with conventional features, (2) VGG19 with conventional features, (3) VGG16 with optimal features, (4) VGG19 with optimal features and (5) concatenated dual-deep-features (DDF). All experimental investigations are conducted and achieved using MATLAB® program. Experimental outcome confirms that the proposed system with DDF yields a classification accuracy of 97.8%using a K Nearest-Neighbor (KNN) classifier.
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Aprendizaje Profundo , Ephemeroptera , Tuberculosis , Algoritmos , Animales , Humanos , Rayos XRESUMEN
The effects of climatic warming on tropical streams have received little attention, and field studies of such changes are generally lacking. Drifting insects from a Hong Kong forest stream were sampled for 36 months between 2013 and 2016, and compared with samples collected using identical methods in 1983-84. Mean air temperatures rose by ~0.5°C (0.17°C per decade) over this period. The stream drained an uninhabited protected area, so no climate-change effects were confounded by anthropogenic disturbance. In total, 105 taxa and >77,000 individuals were collected. Richness of samples in the historic and contemporary datasets did not differ, but true diversity of drifting insects was highest in 1983-84, and declined between 2013-14 and 2015-16. There was considerable disparity in assemblage composition between 1983-84 and 2013-16, and smaller between-year changes in the contemporary dataset. Nine indicator species of the historic dataset were identified. Most were mayflies, particularly Baetidae, which were greatly reduced in relative abundance in 2013-16. Diptera became more numerous, and tanypodine chironomids were the sole contemporary indicator taxon. Relative abundance of eight of 19 drifting species (comprising 60% of total insects) was lower in 2013-16, when the dominant baetid mayfly during 1983-84 had declined by almost 90%; only one of the 19 species occurred at higher abundance. Eight species were affected by seasonal temperature variability, but these responses were not correlated with any tendency to exhibit long-term changes in abundance. Substantial shifts in composition, including declines in mayfly relative abundance and assemblage diversity, occurred after three decades of warming, despite the broad annual range of stream temperatures (~16°C) in Hong Kong. This contradicts the well-known prediction that organisms from variable climates have evolved wider thermal tolerances that reflect prevailing environmental conditions. The observed compositional reorganization indicates that variability, rather than stability, may be typical of undisturbed tropical stream communities.
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Ephemeroptera , Ríos , Animales , Cambio Climático , Bosques , Humanos , InsectosRESUMEN
Species distribution area is determined by both biotic and abiotic factors. In particular, significant geological events influence the biodiversity and the genetic structures of the organisms inhabiting the area. The establishment of physical barriers (e.g., mountains, rivers), drives species differentiation by their interference with biological dispersal or gene flow. The Japanese Islands have a high degree of biodiversity. This study focused on the Kii Peninsula, which stands out as a region of exhibiting particularly high biodiversity, and also exceptionally high endemism. The Kii Peninsula has experienced active mountain formation ever since the Quaternary period. In this study, we investigate the influence of geological events on the establishment of genetic diversity. We focused on the mayfly, Dipteromimus tipuliformis. Phylogenetic analyses were performed utilizing the mitochondrial DNA 16S rRNA and COI regions, and the nuclear DNA histone H3, PEPCK and 28S rRNA regions. As a result, it was shown that this mayfly exhibits a genetic structure that strongly reflects the geological history of the Kii Peninsula, and detected their dispersal process across the Median Tectonic Line. This is a unique and significant study, in that it clearly shows the relationship between the phylogenetic evolution of this mayfly and the corresponding geological history in surprisingly geographic fine scale.
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Ephemeroptera/clasificación , Animales , Biodiversidad , ADN Mitocondrial/química , Ephemeroptera/genética , Variación Genética , Fenómenos Geológicos , Filogenia , Filogeografía , ARN Ribosómico 16S/genéticaRESUMEN
BACKGROUND: Larvae of the Holarctic mayfly genus Rhithrogena Eaton, 1881 (Ephemeroptera, Heptageniidae) are a diverse and abundant member of stream and river communities and are routinely used as bio-indicators of water quality. Rhithrogena is well diversified in the European Alps, with a number of locally endemic species, and several cryptic species have been recently detected. While several informal species groups are morphologically well defined, a lack of reliable characters for species identification considerably hampers their study. Their relationships, origin, timing of speciation and mechanisms promoting their diversification in the Alps are unknown. RESULTS: Here we present a species-level phylogeny of Rhithrogena in Europe using two mitochondrial and three nuclear gene regions. To improve sampling in a genus with many cryptic species, individuals were selected for analysis according to a recent DNA-based taxonomy rather than traditional nomenclature. A coalescent-based species tree and a reconstruction based on a supermatrix approach supported five of the species groups as monophyletic. A molecular clock, mapped on the most resolved phylogeny and calibrated using published mitochondrial evolution rates for insects, suggested an origin of Alpine Rhithrogena in the Oligocene/Miocene boundary. A diversification analysis that included simulation of missing species indicated a constant speciation rate over time, rather than any pronounced periods of rapid speciation. Ancestral state reconstructions provided evidence for downstream diversification in at least two species groups. CONCLUSIONS: Our species-level analyses of five gene regions provide clearer definitions of species groups within European Rhithrogena. A constant speciation rate over time suggests that the paleoclimatic fluctuations, including the Pleistocene glaciations, did not significantly influence the tempo of diversification of Alpine species. A downstream diversification trend in the hybrida and alpestris species groups supports a previously proposed headwater origin hypothesis for aquatic insects.
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Dissimilarities in habitat use, feeding habits, life histories, and physiology can result in syntopic aquatic taxa of similar trophic position bioaccumulating trace elements in vastly different patterns. We compared bioaccumulation in a clam, Corbicula fluminea and mayfly nymph Maccaffertium modestum from a coal combustion waste contaminated stream. Collection sites differed in distance to contaminant sources, incision, floodplain activity, and sources of flood event water and organic matter. Contaminants variably accumulated in both sediment and biofilm. Bioaccumulation differed between species and sites with C. fluminea accumulating higher concentrations of Hg, Cs, Sr, Se, As, Be, and Cu, but M. modestum higher Pb and V. Stable isotope analyses suggested both spatial and taxonomic differences in resource use with greater variability and overlap between species in the more physically disturbed site. The complex but essential interactions between organismal biology, divergence in resource use, and bioaccumulation as related to stream habitat requires further studies essential to understand impacts of metal pollution on stream systems.
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Corbicula/metabolismo , Insectos/metabolismo , Ríos/química , Oligoelementos/metabolismo , Contaminantes Químicos del Agua/metabolismo , Animales , Carbono/metabolismo , Isótopos de Carbono/análisis , Carbón Mineral , Corbicula/química , Ecosistema , Herbivoria , Insectos/química , Nitrógeno/metabolismo , Isótopos de Nitrógeno/análisis , Oligoelementos/análisisRESUMEN
A new species of Labiobaetis Novikova & Kluge, 1987, Labiobaetis soldani sp. nov., is described from the larvae and reared male and female imagoes from Gadana River in the southern Western Ghats in India. Brief ecological notes are appended. The taxonomic status of Labiobaetis is commented on in light of the morphological traits of the larvae and associated imagoes.
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Ephemeroptera/clasificación , Animales , Ephemeroptera/anatomía & histología , Femenino , India , Masculino , Especificidad de la EspecieRESUMEN
The genus Languidipes is currently represented by three species distributed in southeastern Asia, India, and Sri Lanka. Languidipes corporaali is the most widely distributed species, and both, male and female imagos, as well as nymphs, are known. In contrast, the other species, L. taprobanes and L. lithophagus, are only known from nymphs. Here, we describe a new species, Languidipes janae sp nov, based on male imagos collected from Borneo, Indonesia. This new species is characterized by the presence of ommation on mesonotum, and penis almost completely divided, with sub-quadrate base and a small outer projection basal to the long and slender distal arms. This constitutes the first record of the genus for Borneo. A cladistic analysis of the subfamily Asthenopodinae supports its taxonomic status.
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Ephemeroptera , Animales , Borneo , Masculino , Femenino , Ephemeroptera/anatomía & histología , Ephemeroptera/clasificación , Ninfa/clasificación , Ninfa/anatomía & histologíaRESUMEN
Cincticostellajianchuan sp. nov. from Dali Bai Autonomous Prefecture, Yunnan Province, China, is described based on chorionic structure, nymph, and winged stages. The new species is closely related to C.fusca (Kang & Yang, 1995), but it can be distinguished in the male imago stage by its mesonotum and penes morphology, coloration, and the forking point of the stem of MA+Rs on the forewing; in the nymph stage, it can be distinguished by the length of the posterolateral projections of abdominal segment IX and the setation of the abdominal terga. Compared to other congeners, nymphs and male imagoes of the new species and C.fusca share several morphological characteristics, such as a larger body, mesothorax with medially notched anterolateral projections, forefemur without a subapical band of transverse spines of the nymphs, the area between C, Sc and R1 of the forewings distinctly pigmented, and an apical sclerite on the ventral face of the penes of the male imagoes, supporting the proposition of a new species complex, the jianchuan complex. The systematics of Cincticostella and related genera are discussed briefly.
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For the classical multi-objective optimal power flow (MOOPF) problem, only traditional thermal power generators are used in power systems. However, there is an increasing interest in renewable energy sources and the MOOPF problem using wind and solar energy has been raised to replace part of the thermal generators in the system with wind turbines and solar photovoltaics (PV) generators. The optimization objectives of MOOPF with renewable energy sources vary with the study case. They are mainly a combination of 2-4 objectives from fuel cost, emissions, power loss and voltage deviation (VD). In addition, reasonable prediction of renewable power is a major difficulty due to the discontinuous, disordered and unstable nature of renewable energy. In this paper, the Weibull probability distribution function (PDF) and lognormal PDF are applied to evaluate the available wind and available solar power, respectively. In this paper, an enhanced multi-objective mayfly algorithm (NSMA-SF) based on non-dominated sorting and the superiority of feasible solutions is implemented to tackle the MOOPF problem with wind and solar energy. The algorithm NSMA-SF is applied to the modified IEEE-30 and standard IEEE-57 bus test systems. The simulation results are analyzed and compared with the recently reported MOOPF results.
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Agriculture Internet of Things (AIoTs) deployments require design of high-efficiency Quality of Service (QoS) & security models that can provide stable network performance even under large-scale communication requests. Existing security models that use blockchains are either highly complex or require large delays & have higher energy consumption for larger networks. Moreover, the efficiency of these models depends directly on consensus-efficiency & miner-efficiency, which restricts their scalability under real-time scenarios. To overcome these limitations, this study proposes the design of an efficient Q-Learning bioinspired model for enhancing QoS of AIoT deployments via customized shards. The model initially collects temporal information about the deployed AIoT Nodes, and continuously updates individual recurring trust metrics. These trust metrics are used by a Q-Learning process for identification of miners that can participate in the block-addition process. The blocks are added via a novel Proof-of-Performance (PoP) based consensus model, which uses a dynamic consensus function that is based on temporal performance of miner nodes. The PoP consensus is facilitated via customized shards, wherein each shard is deployed based on its context of deployment, that decides the shard-length, hashing model used for the shard, and encryption technique used by these shards. This is facilitated by a Mayfly Optimization (MO) Model that uses PoP scores for selecting shard configurations. These shards are further segregated into smaller shards via a Bacterial Foraging Optimization (BFO) Model, which assists in identification of optimal shard length for underlying deployment contexts. Due to these optimizations, the model is able to improve the speed of mining by 4.5%, while reducing energy needed for mining by 10.4%, improving the throughput during AIoT communications by 8.3%, and improving the packet delivery consistency by 2.5% when compared with existing blockchain-based AIoT deployment models under similar scenarios. This performance was observed to be consistent even under large-scale attacks.
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The genus Afronurus in Thailand is investigated using an integrative approach (morphology based, ootaxonomy and molecular data) for species delimitation. A total of four species of Afronurus was identified; A.cervina (Braasch & Soldán, 1984), A.gilliesiana (Braasch, 1990), A.rainulfiana (Braasch, 1990), and A.rubromaculata (You et al., 1981). The subimago of A.gilliesiana is described for the first time based on reared specimens. The egg structure of all four species is also described for the first time. Morphological and molecular data strongly support their species delimitation. The egg chorionic structure of the genus Afronurus, together with other morphological characters, is useful for species identification. A key to mature nymphs of the known species is provided.
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Siphlonurusdongxi Li & Tong, sp. nov. from Shangri-La City, Yunnan Province, China, is described based on egg, nymph, and winged stages. The new species is closely related to S.davidi (Navás, 1932), and can be distinguished by the colour of the imago, the forking point of MP, the penis, posterolateral spines of tergum IX of imagoes, and first abdominal terga nymph, as well as the structure of the egg. The new species and S.davidi have the same morphological and structural characteristics, such as the long cubital area with many intercalaries, cross veins between C, Sc, RA, and RSa1 surrounded with distinct pigments, the strong curvature of vein CuP in the forewing, the broad expansion of the hindwing, the membranous penis lobes fused without teeth, supporting the proposition of a new species complex, the Siphlonurusdavidi group. The structures of the penis and the egg of the new species could help understand the origin and evolution of the genus Siphlonurus.
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Previously, seven species of the genus Isonychia Eaton, 1871 were reported in China, but they have never been systematically reviewed. After examining our collections from the Chinese mainland, six species and one additional subspecies have been recognized, compared, and photographed. Among them, I.kiangsinensis is redescribed in all stages and a neotype is designated. Its males have triangular penes and nymphs have three dark pigments on each gill. A synonym of I.guixiensisWu et al., 1992 (I.sinensisWu et al., 1992) is confirmed. The males of this species have nearly cylindrical penes and clear abdominal markings. Finally, two species and one subspecies are recorded for the first time in China: I.ussuricasibiricaTiunova et al., 2004, I.ussuricaussurica Bajkova, 1970 and I.vshivkovaevshivkovaeTiunova et al., 2004. Together with the I.ignota (Walker, 1853), I.sexpetalaTiunova et al., 2004, I.formosana (Ulmer, 1912) and possible I.japonica (Ulmer, 1920), they show the rich diversity of the genus Isonychia in China.
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The antioxidant N-(1,3-Dimethylbutyl)-N'-phenyl-p- phenylenediamine (6PPD) is used to protect the rubber in tires from oxidation, which extends the life of the tire. When oxidized, 6PPD is transformed into 6PPD-quinone (6PPDQ). 6PPDQ, along with other tire ingredients, can enter aquatic ecosystems through the transport of tire wear particles in runoff during a precipitation event. The mass mortality of coho salmon following precipitation events in urban areas lead to the discovery that 6PPDQ is the likely cause due to coho salmon's relatively high sensitivity to 6PPDQ. The assessment of 6PPDQ toxicity to other aquatic species has expanded, but it has focused on fish. This study investigated the toxicity of 6PPDQ to four freshwater invertebrate species, larval burrowing mayfly (Hexagenia spp.), juvenile cladoceran (Daphnia magna), file ramshorn snail embryo (Planorbella pilsbryi), and adult washboard mussel (Megalonaias nervosa). For all four species, the highest concentration of 6PPDQ tested did not result in significant mortality. This translated into the determination of the highest concentration that did not cause significant mortality (NOEC) for Hexagenia spp., D. magna, P. pilsbryi, and M. nervosa of 232.0, 42.0, 11.7, and 17.9 µg/L, respectively. The data from this study indicate that freshwater invertebrates are not as sensitive to 6PPDQ as some salmonid species (e.g., coho salmon Oncorhynchus kisutch). This study also analyzed 6PPDQ in road runoff from around the city of Guelph in Ontario, Canada. 6PPQ was detected in all samples but the concentration was two orders of magnitude lower than the NOECs for the four tested species of freshwater invertebrate.
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Benzoquinonas , Agua Dulce , Invertebrados , Fenilendiaminas , Animales , Ecosistema , Ephemeroptera/efectos de los fármacos , Agua Dulce/química , Invertebrados/efectos de los fármacos , Oncorhynchus kisutch , Ontario , Fenilendiaminas/análisis , Fenilendiaminas/toxicidad , Benzoquinonas/análisis , Benzoquinonas/toxicidadRESUMEN
Ecologists have observed declines in the biodiversity of sensitive freshwater organisms in response to increasing concentrations of major ions (salinization). Yet, how changing salinities physiologically challenge aquatic organisms, such as mayflies, remains remarkably understudied. Moreover, it is not well understood the degree to which species respond and acclimate to salinity changes. Our lab is developing the Baetid mayfly, N. triangulifer, as a model organism for physiological research. We have previously described acclimatory changes in both ion flux rates and altered mRNA transcript levels in response to chronic exposures to elevated major ion concentrations at the whole animal level. In the present study, we use shotgun proteomics to identify the specific proteins associated with apical ion transport and how their abundance changes in response to chronic salinity exposures in gills. Gills were isolated from the penultimate nymphal stage of N. triangulifer reared under control culture conditions, elevated NaCl (157 mg L-1 Na), elevated CaCl2 (121 mg L-1 Ca), elevated Ca/MgSO4 (735 mg L-1 SO4). These conditions mirrored those from previously published physiological work. We also acutely exposed nymphs to dilute (50% dilution of culture water with deionized water) to explore proteomic changes in the gills in response to dilute conditions. We report 710 unique peptide sequences among treatment groups, including important apical ion transporters such as Ca-ATPase, Na/K ATPase, and V-ATPase. Treatment with elevated NaCl and Ca/MgSO4 appeared to cause more significant differential protein expression (452 and 345, respectively) compared to CaCl2 and dilute groups (134 and 17, respectively). Finally, we demonstrated the breadth of physiological functions in gills by exploring non-transport related pathways found in our dataset, including ATP synthesis, calcium signaling, and oxidative stress response. We discuss our results in the context of freshwater salinization and the challenges of working with non-model species without fully sequenced and annotated genomes.
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Ephemeroptera , Contaminantes Químicos del Agua , Animales , Branquias/metabolismo , Salinidad , Proteoma/metabolismo , Cloruro de Sodio/metabolismo , Proteómica , Cloruro de Calcio , Contaminantes Químicos del Agua/metabolismo , Organismos Acuáticos/metabolismo , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Iones/metabolismo , Agua/metabolismoRESUMEN
Previously, the euthyplociid mayfly from Thailand was reported as Polyplocia spp. without designation to any nominal species. In this study, the genus Polyplocia Lestage, 1921 in Thailand was reviewed. Two species are recognized: Polyplociaorientalis Nguyen & Bae, 2003 and P.nebulosa Gonçalves & Peters, 2016. This is the first report of P.nebulosa in Continental Asia. Cytochrome c oxidase subunit I (COI) data and illustrations based on nymphal characters were used to confirm two different species in Thailand. Additional morphological characteristics of the nymph and eggs of the two species from Thailand are also shown in detail. The taxonomic status of Polyplocia in the Oriental region is reviewed. Keys to known nymphal and imaginal stages are provided and the distribution of Polyplocia in the Oriental region is discussed.