RESUMEN
Global environmental concerns drive research toward the development of new eco-friendly compounds to replace pollutant chemicals. This study focuses on optimizing the production of trehalose lipids (TLs), which are glycolipid biosurfactants (BS) with various applications like antimicrobial or surface tension reduction. New microorganism sources, growth conditions, medium composition, purification conditions, and physicochemical properties of TLs are studied. Addressing a microscale approach, TLs production was successfully achieved using Rhodotorula sp. and Rhodococcus erythropolis to compare, with different media compositions including glucose-based and salt media supplemented with glycerol, glucose, n-hexadecane, n-dodecane. Liquid-liquid extraction using ethyl acetate and methanol was employed for compound extraction, followed by characterization using analytical methods such as Thin layer chromatography (TLC), High performance liquid chromatography (HPLC), and UHPLC. The produced TLs exhibited a minimum surface tension of 47 mN/m and a critical micellar concentration of 4.4 mg/mL. This study also identified Rhodotorula sp. as a new sustainable producer of TLs with improved productivity.
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Rhodotorula , Trehalosa , Glucolípidos , Micelas , Glucosa , Tensoactivos/químicaRESUMEN
Rhodotorula sp. are well-known for their ability to biosynthesize a diverse range of valuable biomolecules, including carotenoids, lipids, enzymes, and polysaccharides. Despite the high number of studies conducted using Rhodotorula sp. at the laboratory scale, most of these do not address all processual aspects necessary for scaling up these processes for industrial applications. This chapter explores the potential of Rhodotorula sp. as a cell factory for the production of distinct biomolecules, with a particular emphasis on exploring their use from a biorefinery perspective. Through in-depth discussions of the latest research and insights into non-conventional applications, we aim to provide a comprehensive understanding of Rhodotorula sp.'s ability to produce biofuels, bioplastics, pharmaceuticals, and other valuable biochemicals. This book chapter also examines the fundamentals and challenges associated with the optimizing upstream and downstream processing of Rhodotorula sp-based processes. We believe that through this chapter, readers with different levels of expertise will gain insights into strategies for enhancing the sustainability, efficiency, and effectiveness of producing biomolecules using Rhodotorula sp.
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Rhodotorula , Rhodotorula/genética , Carotenoides , Polisacáridos , BiocombustiblesRESUMEN
Biosurfactants (BSs) are known for their remarkable properties, however, their commercial applications are hampered partly by the high production cost. To overcome this issue, a biosurfactant producing strain, Rhodotorula sp.CC01 was isolated using landfill leachate as nitrogen source, while olive oil was determined as the best sole carbon source. The BS produced by Rhodotorula sp.CC01 had oil displacement diameter of 19.90 ± 0.10 cm and could reduce the surface tension of water to 34.77 ± 0.63 mN/m. It was characterized as glycolipids by thin layer chromatography, FTIR spectra, and GC-MS analysis, with the critical micelle concentration of 70 mg/L. Meanwhile, the BS showed stability over a wide range of pH (2-12), salinity (0-100 g/L), and temperature (20-100 °C). During the cultivation process, BS was produced with a maximum rate of 163.33 mg L-1 h-1 and a maximum yield of 1360 mg/L at 50 h. In addition, the removal efficiency of NH4+-N reached 84.2% after 75 h cultivation with a maximum NH4+-N removal rate of 3.92 mg L-1 h-1. Moreover, Rhodotorula sp.CC01 has proven to be of great potential in remediating petroleum hydrocarbons, as revealed by chromogenic assays. Furthermore, genes related to nitrogen metabolism and glycolipid metabolism were found in this strain CC01 after annotating the genome data with KEGG database, such as narB, glycoprotein glucosyltransferase, acetyl-CoA C-acetyltransferase, LRA1, LRA3, and LRA4. The findings of this study prove a cost-effective strategy for the production of BS by yeast through the utilization of landfill leachate.
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Petróleo , Rhodotorula , Contaminantes Químicos del Agua , Biodegradación Ambiental , Hidrocarburos/metabolismo , Nitrógeno/metabolismo , Petróleo/metabolismo , Rhodotorula/genética , Rhodotorula/metabolismo , Tensoactivos/metabolismo , Contaminantes Químicos del Agua/metabolismoRESUMEN
The progress in industrialization everyday life has led to the continuous entry of several anthropogenic compounds, including dyes, into surrounding ecosystem causing arduous concerns for human health and biosphere. Therefore, microbial degradation of dyes is considered an eco-efficient and cost-competitive alternative to physicochemical approaches. These degradative biosystems mainly depend on the utilization of nutritive co-substrates such as yeast extract peptone in conjunction with glucose. Herein, a synergestic interaction between strains of mixed-culture consortium consisting of Rhodotorula sp., Raoultella planticola; and Staphylococcus xylosus was recruited in methylene blue (MB) degradation using agro-industrial waste as an economic and nutritive co-substrate. Via statistical means such as Plackett-Burman design and central composite design, the impact of significant nutritional parameters on MB degradation was screened and optimized. Predictive modeling denoted that complete degradation of MB was achieved within 72 h at MB (200 mg/L), NaNO3 (0.525 gm/L), molasses (385 µL/L), pH (7.5) and inoculum size (18%). Assessment of degradative enzymes revealed that intracellular NADH-reductase and DCIP-reductase were key enzymes controlling degradation process by 104.52 ± 1.75 and 274.04 ± 3.37 IU/min/mg protein after 72 h of incubation. In addition, azoreductase, tyrosinase, laccase, nitrate reductase, MnP and LiP also contributed significantly to MB degradation process. Physicochemical monitoring analysis, namely UV-Visible spectrophotometry and FTIR of MB before treatment and degradation byproducts indicated deterioration of azo bond and demethylation. Moreover, the non-toxic nature of degradation byproducts was confirmed by phytotoxicity and cytotoxicity assays. Chlorella vulgaris retained its photosynthetic capability (Ë 85%) as estimated from Chlorophyll-a/b contents compared to Ë 30% of MB-solution. However, the viability of Wi-38 and Vero cells was estimated to be 90.67% and 99.67%, respectively, upon exposure to MB-metabolites. Furthermore, an eminent employment of consortium either freely-suspended or immobilized in plain distilled water and optimized slurry in a bioaugmentation process was implemented to treat MB in artificially-contaminated municipal wastewater and industrial effluent. The results showed a corporative interaction between the consortium examined and co-existing microbiota; reflecting its compatibility and adaptability with different microbial niches in different effluents with various physicochemical contents.
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Colorantes/metabolismo , Enterobacteriaceae/metabolismo , Azul de Metileno/metabolismo , Modelos Estadísticos , Rhodotorula/metabolismo , Staphylococcus/metabolismo , Animales , Biodegradación Ambiental , Línea Celular , Chlorella vulgaris/metabolismo , Chlorocebus aethiops , Técnicas de Cocultivo , Ecosistema , Humanos , Residuos Industriales , Azul de Metileno/toxicidad , Células Vero , Aguas Residuales/microbiología , Purificación del Agua/métodosRESUMEN
One of the very promising methods in the field of bioremediation of hydrocarbons is the application of biosurfactant- producing microorganisms based on the use of wastewater as renewable substrates of culture media, contributing to the reduction of costs. With this aim, the production, characterization and properties of the yeast strain YBR producing a biosurfactant newly isolated from an oilfield in Algeria, using wastewater from olive oil mills (OOMW) as a substrate for a low-cost and effective production, have been investigated. Screening of biosurfactant production was carried out with different tests, including emulsification index test (E24), drop collapse test, oil spreading technique and measurement of surface tension (ST). The isolated yeast strain was found to be a potent biosurfactant producer with E24 = 69% and a significant reduction in ST from 72 to 35 mN m-1. The study of the cultural, biochemical, physiological and genetic characteristics of the isolate allowed us to identify it as Rhodotorula sp. strain YBR. Fermentation was carried out in a 2.5 L Minifors Bioreactor using crude OOMW as culture medium, the E24 value reached 90% and a reduction of 72 to 35 mN m-1 in ST. A biosurfactant yield = 10.08 ± 0.38 g L-1 was recorded. The characterization by semi-purification and thin layer chromatography (TLC) of the crude extract of biosurfactant showed the presence of peptides, carbohydrates and lipids in its structure. The crude biosurfactant exhibited interesting properties such as: low critical micellar concentration (CMC), significant reduction in ST and strong emulsifying activity. In addition, it has shown stability over a wide range of pH (2-12), temperature (4-100 °C) and salinity (1-10%). More interestingly, the produced biosurfactant has proven to be of great potential application in the remobilization of hydrocarbons from polluted soil with a removal rate of greater than 95%.
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Hidrocarburos/química , Petróleo/microbiología , Rhodotorula/crecimiento & desarrollo , Tensoactivos/metabolismo , Biodegradación Ambiental , Reactores Biológicos/microbiología , Yacimiento de Petróleo y Gas , Filogenia , Rhodotorula/clasificación , Rhodotorula/aislamiento & purificación , Rhodotorula/metabolismoRESUMEN
AIMS: To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel-derived glycerol (Gly) into added-value compounds. METHODS AND RESULTS: Ten newly isolated yeast strains belonging to Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, isolated from fishes, metabolized Gly under nitrogen limitation. The aim of the study was to identify potential newly isolated microbial candidates that could produce single-cell oil (SCO), endopolysaccharides and polyols when these micro-organisms were grown on biodiesel-derived Gly. As controls producing SCO and endopolysaccharides were the strains Rhodotorula glutinis NRRL YB-252 and Cryptococcus curvatus NRRL Y-1511. At initial Gly (Gly0 ) ≈40 g l-1 , most strains presented remarkable dry cell weight (DCW) production, whereas Y. lipolytica and Debaryomyces sp. produced non-negligible quantities of mannitol and arabitol (Ara). Five strains were further cultivated at increasing Gly0 concentrations. Rhodotorula glutinis NRRL YB-252 produced 7·2 g l-1 of lipid (lipid in DCW value ≈38% w/w), whereas Debaryomyces sp. FMCC Y69 in batch-bioreactor experiment with Gly0 ≈80 g l-1 , produced 30-33 g l-1 of DCW and ~30 g l-1 of Ara. At shake-flasks with Gly0 ≈125 g l-1 , Ara of ~48 g l-1 (conversion yield of polyol on Gly consumed ≈0·62 g g-1 ) was achieved. Cellular lipids of all yeasts contained in variable concentrations oleic, palmitic, stearic and linoleic acids. CONCLUSIONS: Newly isolated, food-derived and non-previously studied yeast isolates converted biodiesel-derived Gly into several added-value metabolites. SIGNIFICANCE AND IMPACT OF THE STUDY: Alternative ways of crude Gly valorization through yeast fermentations were provided and added-value compounds were synthesized.
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Biocombustibles/microbiología , Glicerol , Levaduras , Polisacáridos Fúngicos/análisis , Polisacáridos Fúngicos/metabolismo , Glicerol/análisis , Glicerol/metabolismo , Lípidos/análisis , Polímeros/análisis , Polímeros/metabolismo , Levaduras/clasificación , Levaduras/metabolismoRESUMEN
Carotenoids are natural pigments found in plants and microorganisms. These important nutrients play significant roles in animal health. In contrast to plant production, the advantages of microbial fermentation of carotenoids are the lower media costs, fast growth rate of microorganisms, and the ease of culture condition control. In this study, a colony of red pigment-producing yeast, Rhodotorula sp. RY1801, was isolated from the sediment of marine environment with the potential to produce carotenoids. Optimization of carotenoid production in Rhodotorula sp. RY1801 was also discussed. The optimum conditions found for carotenoid production were as follows: temperature, 28 °C; pH 5.0; carbon source, 10 g/L glucose, nitrogen source, 10 g/L yeast extract, maximum concentration of 987 µg/L of total carotenoids was obtained. The results of this study show that the isolated yeast strain Rhodotorula sp. RY1801 can potentially be used in future as a promising microorganism for the commercial production of carotenoids.
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Organismos Acuáticos/metabolismo , Productos Biológicos/metabolismo , Carotenoides/metabolismo , Rhodotorula/metabolismo , Técnicas de Cultivo Celular por Lotes/métodos , Biomasa , Medios de Cultivo/química , FermentaciónRESUMEN
In recent decades, the prognosis for burn patients has improved considerably with the development of specialized care. The acellular dermal matrix (ADM) is a totally artificial acellular device that functions to control water loss, prevent penetration by bacteria and allow migration of endothelial cells and fibroblasts from patient tissues. However, little is known about its effectiveness against yeasts. The present study evaluated the capacity of colonization and migration of some human commensal yeasts. Three clinical isolates from skin scales, identified as Candida parapsilosis, Candida glabrata and Rhodotorula mucilaginosa, were used. Their ability to cross the ADM was evaluated. After three days, all isolates had crossed the ADM. C. parapsilosis showed the lowest growth, while R. mucilaginosa showed intermediate and C. glabrata the highest growth. In the plates incubated for seven days, the growth of C. parapsilosis and C. glabrata increased by 1 log over the third day. All isolates have the capacity to colonize and migrate through the matrix, increasing the potential risk to burn patients, who can develop severe and even fatal infections by invasive fungi.
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Dermis Acelular/microbiología , Quemaduras/complicaciones , Quemaduras/microbiología , Levaduras/crecimiento & desarrollo , Quemaduras/patología , Candida glabrata/crecimiento & desarrollo , Candida glabrata/patogenicidad , Candida parapsilosis/crecimiento & desarrollo , Candida parapsilosis/patogenicidad , Interacciones Huésped-Patógeno , Humanos , Rhodotorula/crecimiento & desarrollo , Rhodotorula/patogenicidad , Factores de Riesgo , Piel/lesiones , Piel/microbiología , Piel/patología , Levaduras/aislamiento & purificación , Levaduras/patogenicidadRESUMEN
The aim of this study was to characterize the yeast microbiota of natural cavities of manatees kept in captivity in Brazil. Sterile swabs from the oral cavity, nostrils, genital opening, and rectum of 50 Trichechus inunguis and 26 Trichechus manatus were collected. The samples were plated on Sabouraud agar with chloramphenicol and incubated at 25 °C for 5 days. The yeasts isolated were phenotypically identified by biochemical and micromorphological tests. Overall, 141 strains were isolated, of which 112 were from T. inunguis (Candida albicans, Candida parapsilosis sensu stricto, Candida orthopsilosis, Candida metapsilosis, Candida guilliermondii, Candida pelliculosa, Candida tropicalis, Candida glabrata, Candida famata, Candida krusei, Candida norvegensis, Candida ciferri, Trichosporon sp., Rhodotorula sp., Cryptococcus laurentii) and 29 were from T. manatus (C. albicans, C. tropicalis, C. famata, C. guilliermondii, C. krusei, Rhodotorula sp., Rhodotorula mucilaginosa, Rhodotorula minuta, Trichosporon sp.). This was the first systematic study to investigate the importance of yeasts as components of the microbiota of sirenians, demonstrating the presence of potentially pathogenic species, which highlights the importance of maintaining adequate artificial conditions for the health of captive manatees.
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Microbiota , Trichechus/microbiología , Animales , Brasil , Candida/aislamiento & purificación , Candida/fisiología , Cryptococcus/aislamiento & purificación , Cryptococcus/fisiología , Femenino , Masculino , Rhodotorula/aislamiento & purificación , Rhodotorula/fisiología , Trichechus inunguis/microbiología , Trichechus manatus/microbiología , Trichosporon/aislamiento & purificación , Trichosporon/fisiologíaRESUMEN
Glycolic acid is an important chemical product widely used in various fields, including cosmetics, detergents, textiles, and more. Currently, microbial production of glycolic acid has disadvantages such as poor genetic stability, low yield, and high cost. Additionally, whole-cell catalytic production of glycolic acid typically requires the addition of relatively expensive sorbitol as a carbon source, which limits its industrial production. To develop an industrially applicable method for glycolic acid production, this study used ethylene glycol as a substrate to screen the glycolic acid-producing strains through whole-cell catalysis, obtaining a Rhodotorula sp. capable of producing glycolic acid. The strain was then subjected to UV mutagenesis and high throughput screening, and the positive mutant strain RMGly-20 was obtained. After optimization in shake flasks, the glycolic acid titer of RMGly-20 reached 17.8 g/L, a 10.1-fold increase compared to the original strain. Using glucose as the carbon source and employing a fed-batch culture in a 5 L fermenter, strain RMGly-20 produced 61.1 g/L of the glycolic acid. This achievement marks the preliminary breeding of a genetically stable glycolic acid-producing strain using a cheap carbon source, providing a new host for the biosynthesis of glycolic acid and promoting further progress toward industrial production.
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Fermentación , Glicolatos , Rhodotorula , Glicolatos/metabolismo , Rhodotorula/metabolismo , Rhodotorula/genética , Microbiología Industrial/métodos , Glicol de Etileno/metabolismo , MutagénesisRESUMEN
Sixty-seven yeast strains were isolated from castor beans then their endogenous lipids were stained by Nile Red (NR) fluorescence dye, and flow cytometry was used to obtain a strain with a high relative mean fluorescence intensity (MFI) value. The highest MFI value was obtained for strain CM33, which produced a maximum lipid content of 20.8 % dry cell weight (DCW). Based on the sequence of the ITS-5.8S-ITS rDNA and D1/D2 26S rDNA regions, CM33 showed 99 % identity with Rhodotorula paludigena. The potential of CM33 to assimilate various carbon sources was examined by growth on minimal media using glucose, glycerol, sucrose or xylose. CM33 was grown in glucose-based medium for 96 h and exhibited a maximum lipid content of 23.9 % DCW. Furthermore, when cells were cultured on molasses waste, their biomass, lipid content and lipid concentration reached 16.5 g/L, 37.1 % DCW and 6.1 g/L, respectively. These results demonstrated the potential of R. paludigena CM33 to contribute to a value-added carbon chain by converting renewable waste materials for biolipid production.
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Rhodotorula , Biomasa , Lípidos , Rhodotorula/genética , LevadurasRESUMEN
Since Aromatic hydrocarbons are recalcitrant and toxic, strategies to remove them are needed. The aim of this work was to isolate fungi capable of using aromatic hydrocarbons as carbon sources. Two isolates from an oil polluted site in Mexico were identified through morphological and molecular markers as a novel Rhodotorula sp. and an Exophiala sp. Both strains were able to grow in a wide range of pH media, from 4 to 12, showing their optimal growth at alkaline pH's and are both halotolerant. The Exophiala strain switched from hyphae to yeast morphotype in high salinity conditions. To the best of our knowledge, this is the first report of salt triggering dimorphism. The Rhodotorula strain, which is likely a new undescribed species, was capable of removing singled ringed aromatic compounds such as benzene, xylene, and toluene, but could not remove benzo[a] pyrene nor phenanthrene. Nevertheless, these hydrocarbons did not impair its growth. The Exophiala strain showed a different removal capacity. It could remove the polyaromatic hydrocarbons but performed poorly at removing toluene and xylene. Nevertheless, it still could grow well in the presence of the aromatic compounds. These strains could have a potential for aromatic compounds removal.
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The goal of this work was to investigate the genetics of an acidophilic phenol-degrading yeast strain using whole-genome sequencing (WGS), characterize the growth of the strain and phenol degradation capability as well as degradation pathway under extremely acidic conditions. The result showed that the strain ZM1 isolated from an acid mine drainage (AMD) belongs to basidiomycetous yeast Rhodotorula sp., which possesses some unique genes compared to other four closely related Rhodotorula species. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that ZM1 possessed the degradation potentials for aromatic compounds. ZM1 was acidophilic with the optimum growth at the initial pH of 3.0. It could adjust pH to desired levels probably by acid production during the cultivation. Notably, at pH 3.0, the strain ZM1 showed a high phenol-degrading capability that almost completely degraded 1100â¯mg/L of phenol in 120â¯h with the highest degradation rate of 0.074â¯g/(g cell dry weight h). Under the same pH, the strain could completely degrade 500â¯mg/L phenol within 48â¯hâ¯at NaCl concentration up to 10â¯g/L. The identification of the gene catA by the KEGG analysis, together with the presence of metabolic intermediate of cis, cis-muconic acid detected by gas chromatography-mass spectrometry, confirmed that the strain ZM1 degraded phenol via ortho-cleavage pathway. These findings suggest that the indigenous yeasts strain ZM1 could be exploited as an important member for in-situ biodegradation of aromatic compounds in the extremely acidic environments.
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Biodegradación Ambiental , Fenol/metabolismo , Rhodotorula/metabolismo , Redes y Vías Metabólicas , Fenoles/metabolismo , LevadurasRESUMEN
The capability of plant growth promoting microbes to survive under abiotic stresses has important significance for improving plant growth and productivity. Among the various plant growth promoting biomolecules produced by microbes, exopolysaccharide (EPS) help microbes to survive in inhospitable environments and endure environmental stressful conditions. In the present study, a yeast strain CAH2 was isolated from Beta vulgaris rhizosphere soil and identified as Rhodotorula sp., based on the partial 18S rRNA gene sequence analysis. Rhodotorula sp. strain CAH2 was found to tolerate higher concentrations of Al (6â¯mM), NaCl (150â¯mM) and PEG-6000 (15%, w/v). The strain CAH2 was shown to produce 7.5â¯gâ¯L-1 of EPS in the production medium with sucrose and yeast extract as a carbon and nitrogen sources, respectively. The EPS yield was increased constantly with increasing concentrations of Al, NaCl and PEG-6000. The structural feature of EPS studied through FT-IR and NMR spectral analysis confirmed the presence of glucose, mannose and galactose. The yeast strain CAH2 was produced multiple plant growth promoting traits in the presence and absence of abiotic stresses. Finally, these results indicate that the production of EPS could be safeguard the plant growth promoting Rhodotorula sp. strain CAH2 from unfavourable environmental conditions.
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Polisacáridos Fúngicos/biosíntesis , Desarrollo de la Planta , Plantas/microbiología , Rhodotorula/fisiología , Estrés Fisiológico , Polisacáridos Fúngicos/química , Rhodotorula/metabolismoRESUMEN
Single cell oil production from sugarcane bagasse hydrolysate by oleaginous yeast Rhodotorula sp. IIP-33 was analyzed using a two stage statistical design approach based on Response Surface Methodology. Variables like pentose sugar, (NH4)2SO4, KH2PO4, yeast extract, pH and temperature were found to influence lipid production significantly. Under optimized condition in a shake flask, yield of lipid was 2.1199 g with fat coefficient of 7.09 which also resembled ~99% similarity to model predicted lipid production. In this paper we are presenting optimized results for production of non polar lipid which could be later deoxygenated into hydrocarbon. A qualitative analyses of selective lipid samples yielded a varying distribution of free acid ranging from C6 to C18, majoring C16:0, C18:0 and C18:1 under different fermentation conditions.
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OBJECTIVE: To evaluate the antifungal activity of four honeys of different types from Algeria against pathogenic yeast i.e. Candida albicans (C. albicans) and Rhodotorula sp. METHODS: Four Algeria honeys of different botanical origin were analyzed to test antifungal effect against C. albicans, and Rhodotorula sp. Different concentrations (undiluted, 10%, 30%, 50% and 70% w/v) of honey were studied in vitro for their antifugal activity using C. albicans and Rhodotorula sp. as fungal strains. RESULTS: The range of the diameter of zone of inhibition of various concentrations of tested honeys was (7-23 mm) for Rhodotorula sp., while C. albicans showed clearly resistance towards all concentrations used. The MICs of tested honey concentrations against C. albicans and Rhodotorula sp. were (70.09-93.48)% and (4.90-99.70)% v/v, respectively. CONCLUSIONS: This study demonstrates that, in vitro, these natural products have clearly an antifungal activity against Rhodotorula sp. and C. albicans.
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Antifúngicos/farmacología , Candida albicans/efectos de los fármacos , Miel , Rhodotorula/efectos de los fármacos , Argelia , Pruebas de Sensibilidad MicrobianaRESUMEN
Objective: To evaluate the antifungal activity of four honeys of different types from Algeria against pathogenic yeast i.e. Candida albicans (C. albicans) and Rhodotorula sp. Methods:Four Algeria honeys of different botanical origin were analyzed to test antifungal effect against C. albicans, and Rhodotorula sp. Different concentrations (undiluted, 10%, 30%, 50%and 70%w/v) of honey were studied in vitro for their antifugal activity using C. albicans and Rhodotorula sp. as fungal strains. Results:The range of the diameter of zone of inhibition of various concentrations of tested honeys was (7-23 mm) for Rhodotorula sp., while C. albicans showed clearly resistance towards all concentrations used. The MICs of tested honey concentrations against C. albicans and Rhodotorula sp. were (70.09-93.48)%and (4.90-99.70)%v/v, respectively. Conclusions:This study demonstrates that, in vitro, these natural products have clearly an antifungal activity against Rhodotorula sp. and C. albicans.
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Los reportes de infecciones micóticas diseminadasdebido a los miembros del orden criptococalesse han limitado al Cryptococcus y Candida. La Rhodotorula es una levadura encontrada en la piel, esputo y excretas de los seres humanos. Morfológica y bioquímicamente se caracteriza por producir un llamativo pigmento rojizo, no asimilar el inositol y tener ocasionalmente rudimentarios pseudomicelios (1-4). Las infecciones causadas por este agente soninfrecuentes en la práctica clínica. Algunos centrosespecializados en cáncer reportan series decasos en las décadas anteriores a 1980 (2).
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Dermatomicosis , Micosis , Rhodotorula/aislamiento & purificación , Rhodotorula/patogenicidad , Anfotericina BRESUMEN
A yeast strain Rhodotorula sp.NJ-298,which was isolated from antarctic sea-ice,could produce carotenoids pigment,and the total production was 1986?g?g~(-1).UV-visible spectrum and LC/MS analysis indicated astaxanthin was the major component of the carotenoids,and HPLC analysis showed its content accounted for 81.9 % of total carotenoids productoin.Maximum astaxanthin production 6506 ?g?L~(-1) was obtained when the yeast was grown at steady-state phase.Optimum temperature for the strain grow was at 8℃,and sodium acetate and peptone were the best carbon and nitrogen sources for the astaxanthin production.Moreover,the pigment had strong activity of scavenging free radicals,and the IC_(50) was 0.40 ?g?mL~(-1) by DPPH assay.