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1.
Molecules ; 23(10)2018 Oct 11.
Artículo en Inglés | MEDLINE | ID: mdl-30314364

RESUMEN

In this study, an improved UPLC-MS (Ultra-high performance liquid chromatography-tandem mass spectrometry) method for simultaneously quantifying twelve major components belonging to two chemical types was developed and validated, and was applied to quantitatively compare the quality of sulfur-fumigated Astragali Radix of different durations and of the fresh reference sample. The results showed that the contents of triterpenes astragaloside III and astragaloside IV decreased moderately, while the flavonoids calycosin, formononetin, and 7,2'-dihydroxy-3',4'-dimethoxyisoflavane decreased significantly. The corresponding flavonoid glycosides increased accordingly, which indicated the occurrence of chemical transformation of flavonoids and glycosides in the process of sulfur-fumigation. These transformations were further confirmed by the synthesis of flavonoid glycosides under simulated sulfur-fumigation circumstances. Furthermore, the sulfur-fumigated duration varied in proportion with the contents of compounds 7, 11, and 12. These results suggest that the established method was precise, accurate and sensitive enough for the global quality evaluation of sulfur-fumigated Astragali Radix. Further, sulfur-fumigation not only changes the proportions of bioactive components, but also causes chemical transformation in Astragali Radix.


Asunto(s)
Medicamentos Herbarios Chinos/análisis , Medicamentos Herbarios Chinos/química , Fumigación , Azufre/química , Astragalus propinquus , Cromatografía Líquida de Alta Presión , Cromatografía Liquida , Glicósidos/química , Estructura Molecular , Reproducibilidad de los Resultados , Solubilidad , Espectrometría de Masas en Tándem
2.
J Pharm Biomed Anal ; 98: 424-33, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25011060

RESUMEN

In this study, a improved high performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous quantification of twelve major components belonging to three chemical types was developed and validated, and was applied to quantitatively compare the quality of Radix Paeoniae Alba (RPA) sulfur-fumigated with different durations and purchased from commercial herbal markets. The contents of paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoic acid and paeonol decreased whereas that of paeoniflorin sulfonate increased in RPA with the extending of sulfur-fumigation duration. Different levels of paeoniflorin sulfonate were determined in ten of seventeen commercial RPA samples, indicating that these ten samples may be sulfur-fumigated with different durations. Moreover, the relative standard deviation of the contents of each component was higher in the commercial sulfur-fumigated RPA samples than that in commercial non-fumigated RPA samples, and the percentage of the total average content of monoterpene glycosides in the determined analytes was higher in the decoctions of commercial sulfur-fumigated RPA than that in commercial non-fumigated RPA samples. All these results suggested that the established method was precise, accurate and sensitive enough for the global quality evaluation of sulfur-fumigated RPA, and sulfur-fumigation can not only change the proportions of bioactive components, but also cause the reduction of the quality consistency of both raw materials and aqueous decoctions of RPA.


Asunto(s)
Paeonia/química , Azufre/química , Acetofenonas/química , Ácido Benzoico/química , Cromatografía Líquida de Alta Presión/métodos , Medicamentos Herbarios Chinos/química , Fumigación/métodos , Glucósidos/química , Glicósidos/química , Medicina de Hierbas/métodos , Monoterpenos/química , Raíces de Plantas/química
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