Gaussian curvature-driven direction of cell fate toward osteogenesis with triply periodic minimal surface scaffolds.

Leaf photosynthesis, coral mineralization, and trabecular bone growth depend on triply periodic minimal surfaces (TPMSs) with hyperboloidal structure on every surface point with varying Gaussian curvatures. However, translation of this structure into tissue-engineered bone grafts is challenging. This article reports the design and fabrication of high-resolution three-dimensional TPMS scaffolds embodying biomimicking hyperboloidal topography with different Gaussian curvatures, composed of body inherent ß-tricalcium phosphate, by stereolithography-based three-dimensional printing and sintering. The TPMS bone scaffolds show high porosity and interconnectivity. Notably, compared with conventional scaffolds, they can reduce stress concentration, leading to increased mechanical strength. They are also found to support the attachment, proliferation, osteogenic differentiation, and angiogenic paracrine function of human mesenchymal stem cells (hMSCs). Through transcriptomic analysis, we theorize that the hyperboloid structure induces cytoskeleton reorganization of hMSCs, expressing elongated morphology on the convex direction and strengthening the cytoskeletal contraction. The clinical therapeutic efficacy of the TPMS scaffolds assessed by rabbit femur defect and mouse subcutaneous implantation models demonstrate that the TPMS scaffolds augment new bone formation and neovascularization. In comparison with conventional scaffolds, our TPMS scaffolds successfully guide the cell fate toward osteogenesis through cell-level directional curvatures and demonstrate drastic yet quantifiable improvements in bone regeneration.

Investigating the Influence of Additive Manufacturing and Ultrasonic Coating Parameters on Biopolymeric Scaffold Performance Using Response Surface Methodology.

Triply periodic minimal surface (TPMS) scaffolds have gained attention in additive manufacturing due to their unique porous structures, which are useful in biomedical applications. Unlike metallic implants that can cause stress shielding, polymeric scaffolds offer a safer alternative. This study is focused on enhancing the compressive strength of additive-manufactured polylactic acid (PLA) scaffolds with a diamond structure. The response surface methodology (RSM)-based experimental design was developed to study the influence of printing parameters. The fused deposition modeling (FDM) process parameters were optimized, achieving a compressive strength of 56.2 MPa. Subsequently, the scaffolds were fabricated at optimized parameters and underwent ultrasonic-assisted polydopamine coating. With the utilization of the RSM approach, the study examined the effects of ultrasonic vibration power, coating solution concentration, and submersion time on compressive strength. The optimal coating conditions led to a maximum compressive strength of 92.77 MPa-a 65.1% improvement over the uncoated scaffold. This enhancement is attributed to the scaffold's porous structure, which enables uniform coating deposition. Energy-dispersive x-ray spectroscopy confirmed the successful polydopamine coating, with 10.64 wt% nitrogen content. These findings demonstrate the potential of ultrasonic-assisted coating in improving the mechanical properties of PLA scaffolds, making them suitable for biomedical applications.

Improvement in Active Cell Proliferation Area at Higher Permeability With Novel TPMS Lattice Structure.

The utilization of lattice-based scaffolds emerging as an advance technique over conventional bio-implants in Bone Tissue Engineering. In this study, totally six lattice structures are considered for permeability and wall shear stress (WSS) investigation. Namely triply periodic minimal surfaces (TPMS)-based Gyroid, Schwarz-P, Schwarz-D, and two beam-based structure-Cubic and Fluorite are compared with the proposed new lattice structure at porosity level of 80%, 75%, and 70%. The proposed new lattice has combine characteristic of Gyroid and Schwarz-D TPMS lattice. The permeability is determined through Darcy's law, where the pressure drop across the lattice structure is calculated using a computational fluid dynamics (CFD) tool at flowrate between 0.2 and 10 ml/min. The Cubic and Schwarz-P lattice structures exhibited the highest permeability but at the cost of a lower active surface area for WSS, measuring below 155 mm2, means least cell proliferation occurs while the permeability value in New Lattice structure is in the ideal range with the enhanced active surface area for WSS (514 mm2). The complex internal curvatures of New Lattice promote the cell proliferation while the through-pore holes allow the efficient cell seeding.

Aflibercept Off-Target Effects in Diabetic Macular Edema: An In Silico Modeling Approach.

Intravitreal aflibercept injection (IAI) is a treatment for diabetic macular edema (DME), but its mechanism of action (MoA) has not been completely elucidated. Here, we aimed to explore IAI's MoA and its multi-target nature in DME pathophysiology with an in silico (computer simulation) disease model. We used the Therapeutic Performance Mapping System (Anaxomics Biotech property) to generate mathematical models based on the available scientific knowledge at the time of the study, describing the relationship between the modulation of vascular endothelial growth factor receptors (VEGFRs) by IAI and DME pathophysiological processes. We also undertook an enrichment analysis to explore the processes modulated by IAI, visualized the effectors' predicted protein activity, and specifically evaluated the role of VEGFR1 pathway inhibition on DME treatment. The models simulated the potential pathophysiology of DME and the likely IAI's MoA by inhibiting VEGFR1 and VEGFR2 signaling. The action of IAI through both signaling pathways modulated the identified pathophysiological processes associated with DME, with the strongest effects in angiogenesis, blood-retinal barrier alteration and permeability, and inflammation. VEGFR1 inhibition was essential to modulate inflammatory protein effectors. Given the role of VEGFR1 signaling on the modulation of inflammatory-related pathways, IAI may offer therapeutic advantages for DME through sustained VEGFR1 pathway inhibition.

A Triply-Periodic-Minimal-Surface Structured Interphase based on Fluorinated Polymers Strengthening High-energy Lithium Metal Batteries.

The challenge of constructing a mechanically robust yet lightweight artificial solid-electrolyte interphase layer on lithium (Li) anodes highlights a trade-off between high battery safety and high energy density. Inspired by the intricate microstructure of the white sea urchin, we first develop a polyvinyl fluoride-hexafluoropropylene (PVDF-HFP) interfacial layer with a triple periodic minimal surface structure (TPMS) that could offer maximal modulus with minimal weight. This design endows high mechanical strength to an ordered porous structure, effectively reduces local current density, polarization, and internal resistance, and stabilizes the anode interface. At a low N/P ratio of ~3, using LiFePO4 as the cathode, Li anodes protected by TPMS-structured PVDF-HFP achieve an extremely low capacity-fading-rate of approximately 0.002 % per cycle over 200 cycles at 1 C, with an average discharge capacity of 142 mAh g-1. Meanwhile, the TPMS porous structure saves 50 wt % of the interfacial layer mass, thereby enhancing the energy density of the battery. The TPMS structure is conducive to large-scale additive manufacturing, which will provide a reference for the future development of lightweight, high-energy-density secondary batteries.

A Devonian crinoid with a diamond microlattice.

Owing to their remarkable physical properties, cellular structures, such as triply periodic minimal surfaces (TPMS), have multidisciplinary and multifunctional applications. Although these structures are observed in nature, examples of TPMS with large length scales in living organisms are exceedingly rare. Recently, microstructure reminiscent of the diamond-type TPMS was documented in the skeleton of the modern knobby starfish Protoreaster nodosus. Here we report a similar microlattice in a 385 Myr old crinoid Haplocrinites, which pushes back the origins of this highly ordered microstructure in echinoderms into the Devonian. Despite the low Mg2+/Ca2+ ratio of the 'calcite' Devonian sea, the skeleton of these crinoids has high-Mg content, which indicates strong biological control over biomineralogy. We suggest that such an optimization of trabecular arrangement additionally enriched in magnesium, which enhances the mechanical properties, might have evolved in these crinoids in response to increased predation pressure during the Middle Palaeozoic Marine Revolution. This discovery illustrates the remarkable ability of echinoderms, through the process of evolutionary optimization, to form a lightweight, stiff and damage-tolerant skeleton, which serves as an inspiration for biomimetic materials.

Three-dimensional membranes for artificial lungs: Comparison of flow-induced hemolysis.

BACKGROUND: Membranes based on triply periodic minimal surfaces (TPMS) have proven a superior gas transfer compared to the contemporary hollow fiber membrane (HFM) design in artificial lungs. The improved oxygen transfer is attributed to disrupting the laminar boundary layer adjacent to the membrane surface known as main limiting factor to mass transport. However, it requires experimental proof that this improvement is not at the expense of greater damage to the blood. Hence, the aim of this work is a valid statement regarding the structure-dependent hemolytic behavior of TPMS structures compared to the current HFM design. METHODS: Hemolysis tests were performed on structure samples of three different kind of TPMS-based designs (Schwarz-P, Schwarz-D and Schoen's Gyroid) in direct comparison to a hollow fiber structure as reference. RESULTS: The results of this study suggest that the difference in hemolysis between TPMS membranes compared to HFMs is small although slightly increased for the TPMS membranes. There is no significant difference between the TPMS structures and the hollow fiber design. Nevertheless, the ratio between the achieved additional oxygen transfer and the additional hemolysis favors the TPMS-based membrane shapes. CONCLUSION: TPMS-shaped membranes offer a safe way to improve gas transfer in artificial lungs.

Electrostatic MEMS Vibration Energy Harvesters inside of Tire Treads.

An electret electrostatic MEMS vibration energy harvester for tire sensors mounted inside of the tire tread is reported. The device was designed so as to linearly change an electrostatic capacitance between the corrugated electret and output electrode according to the displacement of the proof mass. The electromechanical linearity was effective at reducing the power loss. The output power reached 495 µW under sinusoidal vibration despite the footprint size being as small as 1 cm². Under impact vibration inside of the tire tread, the output power reached 60 µW at a traveling speed of 60 km/h. It was revealed that a higher mechanical resonance frequency of the harvester adjusted within the frequency band of a low-power spectral density of impact vibration acceleration was effective for high efficiency harvest impact vibration energy.

Novel 3D printed TPMS scaffolds: microstructure, characteristics and applications in bone regeneration.

Bone defect disease seriously endangers human health and affects beauty and function. In the past five years, the three dimension (3D) printed radially graded triply periodic minimal surface (TPMS) porous scaffold has become a new solution for repairing bone defects. This review discusses 3D printing technologies and applications for TPMS scaffolds. To this end, the microstructural effects of 3D printed TPMS scaffolds on bone regeneration were reviewed and the structural characteristics of TPMS, which can promote bone regeneration, were introduced. Finally, the challenges and prospects of using TPMS scaffolds to treat bone defects were presented. This review is expected to stimulate the interest of bone tissue engineers in radially graded TPMS scaffolds and provide a reliable solution for the clinical treatment of personalised bone defects.

Optimization of functionally graded solid-network TPMS meta-biomaterials.

The current study enhances the performance of solid-network triply periodic minimal surface (TPMS) cellular materials through using cell size grading along with the Taguchi method. Cell size grading is a novel technique used to control the size of pores and the surface area without changing the relative density. In this context, experimental compression testing was conducted on six distinct geometries of cell size graded TPMS structures (Diamond, Fischer-Koch S, Gyroid, IWP, Primitive, and Schoen-F-RD) manufactured with dental resin using a masked stereolithography (MSLA) printer. The findings indicated that mean total energy absorption was greater for smaller initial cell sizes (4 and 6 mm) compared to larger sizes (12 mm). Consistent patterns were also observed with respect to final cell sizes. Upon examination of the stress-strain relationships between D and I-WP, it is evident that D exhibits a higher initial peak stress point. However, subsequent to a significant decline, it exhibits a tremendous degree of volatility before recovering. Conversely, I-WP demonstrated greater stability throughout the experiments, with a notably greater maximum stress effect. A significant influence was observed from the initial cell size on stress, with larger sizes leading to a reduction in absorbed energy. The acquired results serve as an essential basis for the identification of optimized designs that may be implemented to enhance the structures' durability.

Exploring the optimal mechanical properties of triply periodic minimal surface structures for biomedical applications: A Numerical analysis.

Currently, cutting-edge Additive Manufacturing techniques, such as Selective Laser Melting (SLM) and Electron Beam Melting (EBM), offer manufacturers a valuable avenue, especially in biomedical devices. These techniques produce intricate porous structures that draw inspiration from nature, boast biocompatibility, and effectively counter the adverse issues tied to solid implants, including stress shielding, cortical hypertrophy, and micromotions. Within the domain of such porous structures, Triply Periodic Minimal Surface (TPMS) configurations, specifically the Gyroid, Diamond, and Primitive designs, exhibit exceptional performance due to their bioinspired forms and remarkable mechanical and fatigue properties, outshining other porous counterparts. Consequently, they emerge as strong contenders for biomedical implants. However, assessing the mechanical properties and manufacturability of TPMS structures within the appropriate ranges of pore size, unit cell size, and porosity tailored for biomedical applications remains paramount. This study aims to scrutinize the mechanical behavior of Gyroid, Diamond, and Primitive structures in solid and sheet network iterations within the morphological parameter ranges suitable for tasks like cell seeding, vascularization, and osseointegration. A comparison with the mechanical characteristics of host bones is also undertaken. The methodology revolves around Finite Element Method (FEM) analysis. The six structures are originally modeled with unit cell sizes of 1, 1.5, 2, and 2.5 mm, and porosity levels ranging from 50% to 85%. Subsequently, mechanical properties, such as elasticity modulus and yield strength, are quantified through numerical analysis. The results underscore that implementing TPMS designs enables unit cell sizes between 1 and 2.5 mm, facilitating pore sizes within the suitable range of approximately 300-1500 µm for biomedical implants. Elasticity modulus spans from 1.5 to 33.8 GPa, while yield strength ranges around 20-304.5 MPa across the 50%-85% porosity spectrum. Generally, altering the unit cell size exhibits minimal impact on mechanical properties within the range above; however, it's noteworthy that smaller porosities correspond to heightened defects in additively manufactured structures. Thus, for an acceptable pore size range of 500-1000 µm and a minimum wall thickness of 150 µm, a prudent choice would involve adopting a 2.5 mm unit cell size.

Biological Properties of 3D-Printed Zirconia Implants with p-Cell Structures.

Research on 3-dimensional (3D) printed porous zirconia-based dental implants is still in its infancy. This study aimed to evaluate the biological responses of novel zirconia implants with p-cell structures fabricated by 3D printing. The solid zirconia samples exhibited comparable density, 3-point flexural strength, and accelerated aging properties compared to specimens prepared previously by conventional methods. Cell-based experiments showed that the p-cell structure promoted cell proliferation, adhesion, and osteogenesis-related protein expression. Mechanical tests showed that both p-cell and control implants could withstand a torque of 35 Ncm without breaking. The mean maximum breaking loads of p-cell and control implants were 1,222.429 ± 115.591 N and 1,903.857 ± 250.673 N, respectively, which were much higher than the human physiological chewing force and human mean maximum occlusal force. An animal experiment showed that the bone trabeculae around the implants were significantly thicker, more numerous, and denser in the p-cell group than in the control group. This work could provide promising guidance for further exploring 3D printing techniques for porous zirconia bionic implants in dentistry.

Multidisciplinary design optimisation of lattice-based battery housing for electric vehicles.

Batteries with high energy densities become essential with the increased uptake of electric vehicles. Battery housing, a protective casing encapsulating the battery, must fulfil competing engineering requirements of high stiffness and effective thermal management whilst being lightweight. In this study, a graded lattice design framework is developed based on topology optimisation to effectively tackle the multidisciplinary objectives associated with battery housing. It leverages the triply periodic minimal surfaces lattices, aiming for high mechanical stiffness and efficient heat dissipation considering heat conduction and convection. The effectiveness of the proposed framework was demonstrated through the battery housing design, showcasing its ability to address multidisciplinary objectives as evidenced by the analysis of the Pareto front. This study identifies the potential of lattices in lightweight applications incorporating multiphysics and offers an efficient lattice design framework readily extended to other engineering challenges.

Designs and mechanical responses of 3D-printed Ti6Al4V porous structures based on triply periodic minimal surfaces with different iso-values.

With the increasing applications of additive manufacturing in orthopaedic implants and numerous designs of porous structures available, there is a strong need and opportunity to optimize the structure designs for improved bone integration. Here we created a unique group of sheet structures based on triply periodic minimal surface (TPMS) by varying the iso-value and systematically examined how iso-value influences the mechanical performance of sheet diamond TPMS structures compared to the Octet truss structure. Four iso-values (C) 0, 0.25, 0.5, and 0.75 were designed for sheet Diamond (OSD) TPMS with varying porosity, and Ti6Al4V powder bed fusion was used to produce the porous structures. Compressive tests revealed that iso-value C significantly affected mechanical performance, and interestingly, the impact was porosity-dependent. At high relative density (>0.25), OSD0 (C = 0) displayed the highest elastic modulus and yield strength, whereas at low relative density (<0.25), OSD0.5 showed the highest among all OSD structures. Regarding failure mechanisms, OSD0, OSD0.25, and OSD0.75 showed a mixed domination of stretching and bending, while OSD0.5 was predominantly stretching-dominated. Finite Element Analysis (FEA) found that local yielding initiated at cell nodes upon loading, followed by surface bending and the formation of single or multiple shear bands near the cell nodes. This work demonstrated the feasibility of improving the mechanical performance of porous TPMS structures by simple adjustments in their governing trigonometric functions, serving as a starting point to customize porous structures for specific applications.

Influence of structural parameters of 3D-printed triply periodic minimal surface gyroid porous scaffolds on compression performance, cell response, and bone regeneration.

In this study, multi-scale triply periodic minimal surface (TPMS) porous scaffolds with uniform and radial gradient distribution on pore size were printed based on the selective laser melting technology, and the influences of porosity, pore size and radial pore size distribution on compression mechanical properties, cell behavior, and bone regeneration behavior were analyzed. The results showed that the compression performance of the uniform porous scaffolds with high porosity was similar to that of cancellous bone of pig tibia, and the gradient porous scaffolds have higher elastic modulus and compressive toughness. After 4 days of cell culture, cells were distributed on the surface of scaffolds mostly, and the number of adherent cells was higher on the small pore size porous scaffolds; After 7 days, the area and density of cell proliferation on the scaffolds were improved; After 14 days, the cells on the small pore size scaffolds tended to migrate to adjacent pores. Animal implantation experiments showed that collagen fiber osteoid was intermittent on scaffolds with high porosity and large pore size, which was not conducive to bone formation. The appropriate pore size and porosity of bone regeneration were 792 um and 83%, respectively, and the regenerative ability of gradient pore size was better than that of uniform pore size. Our study explains the rules of TPMS gyroid structure parameters on compression performance, cell response and bone regeneration, and provides a reference value for the design of bone repair scaffolds for clinical orthopedics.

Biomolecule-grafted GO enhanced the mechanical and biological properties of 3D printed PLA scaffolds with TPMS porous structure.

Graphene oxide (GO) exhibits excellent mechanical strength and modulus. However, its effectiveness in mechanically reinforcing polymer materials is limited due to issues with interfacial bonding and dispersion arising from differences in the physicochemical properties between GO and polymers. Surface modification using coupling agents is an effective method to improve the bonding problem between polymer and GO, but there may be biocompatibility issues when used in the biomedical field. In this study, the biomolecule L-lysine, was applied to improve the interfacial bonding and dispersion of GO in polylactic acid (PLA) without compromising biocompatibility. The PLA/L-lysine-modified GO (PLA/L-GO) bone scaffold with triply periodic minimal surface (TPMS) structure was prepared using fused deposition modeling (FDM). The FTIR results revealed successful grafting of L-lysine onto GO through the reaction between their -COOH and -NH2 groups. The macroscopic and microscopic morphology characterization indicated that the PLA/L-GO scaffolds exhibited an characteristics of dynamic diameter changes, with good interlayer bonding. It was noteworthy that the L-lysine modification promoted the dispersion of GO and the interfacial bonding with the PLA matrix, as characterized by SEM. As a result, the PLA/0.1L-GO scaffold exhibited higher compressive strength (13.2 MPa) and elastic modulus (226.8 MPa) than PLA/0.1GO. Moreover, PLA/L-GO composite scaffold exhibited superior biomineralization capacity and cell response compared to PLA/GO. In summary, L-lysine not only improved the dispersion and interfacial bonding of GO with PLA, enhancing the mechanical properties, but also improved the biological properties. This study suggests that biomolecules like L-lysine may replace traditional modifiers as an innovative bio-modifier to improve the performance of polymer/inorganic composite biomaterials.

Multi-parameter design of triply periodic minimal surface scaffolds: from geometry optimization to biomechanical simulation.

This study introduces a multi-parameter design methodology to create triply periodic minimal surface (TPMS) scaffolds with predefined geometric characteristics. The level-set constant and unit cell lengths are systematically correlated with targeted porosity and minimum pore sizes. Network and sheet scaffolds featuring diamond, gyroid, and primitive level-set structures are generated. Three radially graded schemes are applied to each of the six scaffold type, accommodating radial variations in porosity and pore sizes. Computer simulations are conducted to assess the biomechanical performance of 18 scaffold models. Results disclose that diamond and gyroid scaffolds exhibit more expansive design ranges than primitive counterparts. While primitive scaffolds display the highest Young's modulus and permeability, their lower yield strength and mesenchymal stem cell (MSC) adhesion render them unsuitable for bone scaffolds. Gyroid scaffolds demonstrate superior mechanical and permeability performances, albeit with slightly lower MSC adhesion than diamond scaffolds. Sheet scaffolds, characterized by more uniform material distribution, exhibit superior mechanical performance in various directions, despite slightly lower permeability. The higher specific surface area of sheet scaffolds contributes to elevated MSC adhesion. The stimulus factor analysis also revealed the superior differentiation potential of sheet scaffolds over network ones. The diamond sheet type demonstrated the optimal differentiation. Introducing radial gradations enhances axial mechanical performance at the expense of radial mechanical performance. Radially decreasing porosity displays the highest permeability, MSC adhesion, and differentiation capability, aligning with the structural characteristics of human bones. This study underscores the crucial need to balance diverse biomechanical properties of TPMS scaffolds for bone tissue engineering.

Comparing ceramic Fischer-Koch-S and gyroid TPMS scaffolds for potential in bone tissue engineering.

Triply Periodic Minimal Surfaces (TPMS), such as Gyroid, are widely accepted for bone tissue engineering due to their interconnected porous structures with tunable properties that enable high surface area to volume ratios, energy absorption, and relative strength. Among these topologies, the Fischer-Koch-S (FKS) has also been suggested for compact bone scaffolds, but few studies have investigated these structures beyond computer simulations. FKS scaffolds have been fabricated in metal and polymer, but to date none have been fabricated in a ceramic used in bone tissue engineering (BTE) scaffolds. This study is the first to fabricate ceramic FKS scaffolds and compare them with the more common Gyroid topology. Results showed that FKS scaffolds were 32% stronger, absorbed 49% more energy, and had only 11% lower permeability than Gyroid scaffolds when manufactured at high porosity (70%). Both FKS and Gyroid scaffolds displayed strength and permeability in the low range of trabecular long bones with high reliability (Weibull failure probability) in the normal direction. Fracture modes were further investigated to explicate the quasi-brittle failure exhibited by both scaffold topologies, exploring stress-strain relationships along with scanning electron microscopy for failure analysis. Considering the physical aspects of successful bone tissue engineering scaffolds, FKS scaffolds appear to be more promising for further study as bone regeneration scaffolds than Gyroid due to their higher compressive strength and reliability, at only a small penalty to permeability. In the context of BTE, FKS scaffolds may be better suited than Gyroids to applications where denser bone and strength is prioritized over permeability, as suggested by earlier simulation studies.

Purification of his-tagged proteins using printed monolith adsorption columns.

Bio-separation is a crucial process in biotechnology and biochemical engineering for separating biological macromolecules, and the field has long relied on bead-based and expanded bed chromatography. Printed monolith adsorption (PMA) is a new alternative to which uses a 3D-printed monolithic structure containing self-supporting, ordered flow channels. PMA allows for direct purification of biological molecules from crude cell lysates and cell cultures, and like the other technologies, can functionalized to specifically target a molecule and enable affinity chromatography. Here we have combined PMA technology with an immobilized metal affinity ligand (iminodiacetic acid) to provide selectivity of binding to polyhistidine-tagged proteins during PMA chromatography. Two different PMA structures were created and tested for both static and dynamic protein-binding capacity. At comparative linear flow rates, the dynamic binding capacity of both columns was ≈3 mg/mL, while static capacity was shown to differentiate based on column voidage. We show that a polyhistidine-tagged protein can be directly purified from crude lysate with comparable results to the available commercial providers of IMAC, and with a substantially reduced purification time.

TPMS Microarchitectures for Vertical Bone Augmentation and Osteoconduction: An In Vivo Study.

Triply periodic minimal surface microarchitectures (TPMS) were developed by mathematicians and evolved in all kingdoms of living organisms. Renowned for their lightweight yet robust attributes, TPMS structures find application in diverse fields, such as the construction of satellites, aircrafts, and electric vehicles. Moreover, these microarchitectures, despite their intricate geometric patterns, demonstrate potential for application as bone substitutes, despite the inherent gothic style of natural bone microarchitecture. Here, we produced three TPMS microarchitectures, D-diamond, G-gyroid, and P-primitive, by 3D printing from hydroxyapatite. We explored their mechanical characterization and, further, implanted them to study their bone augmentation and osteoconduction potential. In terms of strength, the D-diamond and G-gyroid performed significantly better than the P-primitive. In a calvarial defect model and a calvarial bone augmentation model, where osteoconduction is determined as the extent of bony bridging of the defect and bone augmentation as the maximal vertical bone ingrowth, the G-gyroid performed significantly better than the P-primitive. No significant difference in performance was observed between the G-gyroid and D-diamond. Since, in real life, the treatment of bone deficiencies in patients comprises elements of defect bridging and bone augmentation, ceramic scaffolds with D-diamond and G-gyroid microarchitectures appear as the best choice for a TPMS-based scaffold in bone tissue engineering.