RESUMEN
It is still unclear whether toll-like receptor (TLR) signaling and serum levels of inflammatory markers in metabolically unhealthy abdominally obese (MUAO) are due to their obesity and/or their metabolic state. We hypothesized that abdominal obesity is an important mediator of the association of metabolic state with TLR signaling and serum inflammatory markers. Therefore, in this case-control study, we compared the expression levels of TLR4 and Toll/interleukin-1 receptor domain containing adaptor protein-inducing interferon ß (TRIF) and serum concentrations of interferon ß and lipoprotein-binding protein (LBP) in metabolically healthy abdominally obese (MHAO) and MUAO individuals. Basal blood samples from 65 abdominally obese subjects with waist circumference (WC) of at least 95 cm were collected to determine serum metabolic parameters, IFNß, and LBP. Those with 3 or more metabolic alterations were defined as MUAO (nâ¯=â¯34), and those having 2 or less were classified as MHAO (nâ¯=â¯31). Furthermore, messenger RNA (mRNA) was isolated from peripheral blood mononuclear cells. TLR4 and TRIF gene expression assay was performed using quantitative real-time polymerase chain reaction. There were significant differences in serum fasting blood sugar (Pâ¯=â¯.017), triglyceride (Pâ¯<â¯.001), cholesterol (Pâ¯=â¯.002), and low-density lipoprotein cholesterol (Pâ¯=â¯.034) between the MUAO and MHAO groups, whereas no significant difference was observed in the expression ratio of TLR4 and TRIF mRNA and serum levels of IFNß and LBP. However, a significant correlation was noticed between mRNA expression levels of TLR4 and TRIF (râ¯=â¯0.50, Pâ¯<â¯.001) and serum IFNß and LBP (râ¯=â¯0.70, Pâ¯<â¯.001). It is concluded that the expression levels of TLR4 and TRIF as well as serum IFNß and LBP are more related to abdominal obesity than to metabolic health.