Comparing the variation and influencing factors of CO2 emission from subsidence waterbodies under different restoration modes in coal mining area.

Subsidence waterbodies play an important role in carbon cycle in coal mining area. However, little effort has been made to explore the carbon dioxide (CO2) release characteristics and influencing factors in subsidence waterbodies, especially under different restoration modes. Here, we measured CO2 release fluxes (F(CO2)) across Anguo wetland (AW), louts pond (LP), fishpond (FP), fishery-floating photovoltaic wetland (FFPV), floating photovoltaic wetland (FPV) in coal mining subsidence area, with unrestored subsidence waterbodies (SW) and unaffected normal Dasha river (DR) as the control area. We sampled each waterbody and tested which physical, chemical, and biological characteristics of water and sediment related to variability in CO2. The results indicated that F(CO2) exhibited the following patterns: FFPV > FPV > FP > SW > DR > LP > AW. Trophic lake index (TLI) and microbial biomass carbon content (MBC) in sediment had a positive impact on F(CO2). The dominant archaea Euryarchaeota and Thaumarchaeota, and dominant bacteria Proteobacteria promoted F(CO2). This study can help more accurately quantify CO2 emissions and guide CO2 future emission reduction and subsidence waterbodies estoration.

Combining hydrochemistry and 13C analysis to reveal the sources and contributions of dissolved inorganic carbon in the groundwater of coal mining areas, in East China.

East China is a highly aggregated coal-grain composite area where coal mining and agricultural production activities are both flourishing. At present, the geochemical characteristics of dissolved inorganic carbon (DIC) in groundwater in coal mining areas are still unclear. This study combined hydrochemical and carbon isotope methods to explore the sources and factors influencing DIC in the groundwater of different active areas in coal mining areas. Moreover, the 13C isotope method was used to calculate the contribution rates of various sources to DIC in groundwater. The results showed that the hydrochemical types of groundwater were HCO3-Ca·Na and HCO3-Na. The main water‒rock interactions were silicate and carbonate rock weathering. Agricultural areas were mainly affected by the participation of HNO3 produced by chemical fertilizer in the weathering of carbonate rocks. Soil CO2 and carbonate rock weathering were the major sources of DIC in the groundwater. Groundwater in residential areas was primarily affected by CO2 from the degradation of organic matter from anthropogenic inputs. Sulfate produced by gypsum dissolution, coal gangue accumulation leaching and mine drainage participated in carbonate weathering under acidic conditions, which was an important factor controlling the DIC and isotopic composition of groundwater in coal production areas. The contribution rates of groundwater carbonate weathering to groundwater DIC in agricultural areas and coal production areas ranged from 57.46 to 66.18% and from 54.29 to 62.16%, respectively. In residential areas, the contribution rates of soil CO2 to groundwater DIC ranged from 51.48 to 61.84%. The results will help clarify the sources and circulation of DIC in groundwater under the influence of anthropogenic activities and provide a theoretical reference for water resource management.

Study of geochemical features of soils on the territory of an abandoned coal mining area using geoinformation technologies.

The article describes the results of a study of the geochemical features of soils on the territory of an abandoned mining area. The Kizel coal basin (Russia) is of particular interest for studying the consequences of technogenic and post-technogenic transformation of the natural environment. The study of the soil as a deposit medium made it possible to identify geochemical indicators of the negative impact. Such a detailed study of the distribution of chemical elements in this area was conducted for the first time. Geoinformation system and maps with interpolation were created to study the spatial distribution of metals and metalloids in soils. Umbric Retisols Abruptic and Haplic Retisols Abruptic soils are common in the territory. Sampling for geochemical testing was carried out from two horizons: humus and podzolic. Sampling from two depths made it possible to identify elements that continue to be contaminated at the time of the study. A total of 103 sample plots were established in the study area. The results obtained were compared with the background of the natural region of the Western Urals to identify the contribution of technogenesis. As a result, the coefficients of concentration and dispersion of chemical elements were calculated. Due to this, elements were identified, the accumulation of which occurs on the territory of the Kizelovsky coal basin. To identify the current and accumulated pollution, the ratio between the humus and podzolic horizons was calculated. As a result, it was found that at the moment in the humus horizon in some areas there is a high accumulation of Co, Mn, Ni and Sr. The geochemical series of the territory for the humus and podzolic horizons was obtained: Fe > Ti > Mn > Sr > Cr > V > Zn > Ni > Co > Pb > As. Data on the geochemical specificity of the territory of the Kizel coal basin have been obtained. The created geoinformation database reflects the physical and chemical properties of soils, metals and metalloids content, dispersion and accumulation coefficients, coefficients of the ratio of the humus and podzolic horizon. Based on it, it is possible to obtain data on the geochemical features of the territory, geoecological characteristics, spatial distribution of metals and metalloids and identification of pollution sources. Co (24 ± 2.8 mg/kg), Mn (1100 ± 155 mg/kg), Ni (69 ± 9.3 mg/kg), As (10 ± 3.5 mg/kg), Cr (178 ± 20 mg/kg), Zn (80 ± 7.8 mg/kg) and Sr (221 ± 26 mg/kg) accumulate in the humus horizon. Co (24 ± 1.8 mg/kg), Mn (1000 ± 103 mg/kg), Ni (60 ± 6.4 mg/kg) and Cr (153 ± 15.2 mg/kg) accumulate in the podzolic horizon.

Environmental geochemical characteristics of rare-earth elements in surface waters in the Huainan coal mining area, Anhui Province, China.

This study investigated the environmental geochemical characteristics of rare-earth elements (REEs) in surface waters in the Huainan mining area, Anhui Province, China. The REEs concentrations were determined by ICP-MS, and the inorganic species of dissolved REEs in the river and coal mining subsidence area water samples were calculated by using the Visual MINTEQ (version 3.1) code. On this basis, the distribution and geochemical characteristics of REEs in the surface waters were systematically analyzed, and the main inorganic species of REEs were investigated. The results showed the following: (1) The REEs concentrations in the surface waters were relatively low, ranging from 0.1361 to 0.3536 µg/L, and the average ∑REEs concentration was 0.2062 µg/L. Compared with light rare-earth elements (LREEs), heavy rare-earth elements (HREEs) were significantly enriched, with an average enrichment factor of 1.4642. Due to the interaction of high pH values and high cation concentrations, the ∑REEs content in the subsidence area water was significantly lower than that in the river water. (2) The distribution pattern of REEs in the surface waters normalized against the North American Shale Composite (NASC) showed that the REEs in the study area had different degrees of cerium (Ce) and europium (Eu) anomalies. The negative Ce anomalies were probably closely related to the pH conditions, whereas the positive Eu anomalies were mainly attributed to preferential chemical weathering and the dissolution of feldspar minerals. (3) The simulation results obtained by using Visual MINTEQ code showed that the dominant and typically inorganic complex form of REEs in the surface waters was carbonate complexes, and this form was one of the reasons for the enrichment of HREEs in the surface water.

Pollution characteristics and probabilistic health risk of potentially hazardous elements in soils near a typical coal mine in Panzhihua City, Southwest China.

This study first assessed the pollution characteristics and probabilistic health risks of potentially hazardous elements (PHEs) in soils from the Dabaoding coal mining area in southwest China using Monte Carlo simulation. Experimental results showed that Cd was moderately enriched in soils, while Ni, Cr, and V were slightly enriched. However, the geoaccumulation index (Igeo) illustrated that the coal mining area had a low level of Cd pollution. PHEs produced a very high ecological risk to soils in the coal mining area, whereas Cd showed the highest contribution (82.56%). The mean hazard index of all soil PHEs was 7.45E - 02 and 4.18E - 01 for local adults and children, respectively, all of which were obviously lower than the maximum acceptable level of 1.0. However, Monte Carlo simulation analysis indicated that 1.08% of noncarcinogenic risk values for local children still exceeded the maximum acceptable level. Additionally, 10.84% and 18.40% of the total carcinogenic risk values for local adults and children, respectively, exceeded the threshold of 1E - 04. Indeed, Cr and Ni had the highest contributions to noncarcinogenic and carcinogenic risks, respectively. These findings suggest that Cd, Cr, and Ni should be identified as priority pollutants in coal mining areas. This study also provides valuable implications for policy-makers and environmental engineers, proposing efficient policies for better soil pollution control and remediation strategies in coal mining areas.

Soil moisture assessment through the SSMMI and GSSIM algorithm based on SPOT, WorldView-2, and Sentinel-2 images in the Daliuta Coal Mining Area, China.

A set of indicators that focus only on numerical values is constructed based on remotely sensed images to assess soil moisture conditions. The quantitative evaluation of soil moisture variation in two periods is rarely referred to in the current literature. In this study, a scaled soil moisture monitoring index (SSMMI) was established to monitor the soil moisture status during 2010-2018 in the Daliuta Coal Mining Area (DCMA), China, based on SPOT-5, SPOT-6, and Sentinel-2 images. We also employed a gradient-based structural similarity (GSSIM) algorithm to quantitatively analyze the characteristics of the spatial distribution of the soil moisture in the DCMA. The optimal scale for exploring the spatial heterogeneity of the soil moisture was determined by local variance and semivariance methods. The results showed that the soil moisture decreased at a rate of 0.0213/a from 2010 to 2018. The areas with the extremely dry and dry levels, which were mainly located in the northwest, some regions of the central area, and the southeast of the DCMA, decreased from 14.48% in 2010 to 13.66% in 2018. The proportion of the no dry level was improved by 14.62%, while the area of the extremely wet and wet levels decreased by 13.79%. The mean value of the soil moisture in the unmined area was greater than that in the DCMA, which was larger than that in the mined area. The result of the GSSIM analysis indicated that the area of dramatic change, where the soil moisture changed substantially, was chiefly distributed in the north, west, some central regions, and some parts of the south and east of the DCMA. The region where the substantial change occurred was surrounded by a moderate-change area, which was encompassed by a low-change area. The area with dramatic and moderate decreases in the soil moisture accounted for 64.52% of the region, which was greater than that with incremental soil moisture changes, which accounted for 5.85% of the region. The area also showed decreased soil moisture from 2010 to 2018. Soil moisture changes are closely related to variations in land cover. For instance, vegetative cover over an open-pit mining area can cause a dramatic reduction in soil moisture. Ninety-three meters was the optimal scale used for monitoring the soil moisture in the DCMA, which indicates that we can adopt the SPOT-5, SPOT-6, and Sentinel-2 images to evaluate the soil moisture conditions in the DCMA.

Health risk assessment of potentially harmful elements in subsidence water bodies using a Monte Carlo approach: An example from the Huainan coal mining area, China.

Enrichment of potentially harmful elements in surface water results in ecological risk to the surrounding environment. Assessing the environmental risk of these elements is of great importance. In this study, surface water samples from 6 different subsidence water bodies in the Huainan coal mining area were collected. The concentrations of Cu, Ni, Pb, Cd, Co, Cr, V, Fe, Mn and Zn were measured by atomic absorption spectrophotometry, and those of As and Hg were analyzed by atomic fluorescence spectrometry. Then, human health risks through the ingestion and dermal contact pathways were assessed and analyzed on the basis of a Monte Carlo simulation. The mean and 95th percentile risks were reported. The results showed that the total carcinogenic risk values in every subsidence water body summed for Cr, Ni and As via two exposure pathways were greater than the maximum acceptable level (1 × 10-4), and Xinji'er water body had the highest carcinogenic risk. Among three elements, Ni was the highest contributor to carcinogenic risk. All non-carcinogenic health risk (hazard quotients) values except for one water area of Co (Xinji'er) were less than 1; however, the total non-carcinogenic health risks of two water bodies (Xinji'er, Xinjiyi) summed for all the elements based on mean concentrations were higher than 1. Xinji'er had the highest hazard index. The extent of the impacts of the total hazard quotients followed the order of Co > As > Cd > Hg > Pb > V >Fe > Ni > Mn > Zn > Cr. Furthermore, the total hazard quotients of Co and As via ingestion pathway summed for the six subsidence water areas were greater than 1, which should be a concern.

Heavy metals in the soils and plants from a typical restored coal-mining area of Huainan coalfield, China.

This study was conducted to pursue the heavy metals in the soil and plants of a typical restored coal-mining area, China. The average concentrations of Cu, Zn, Cr, Ni, and Pb in soil were 26.4, 76.1, 188.6, 34.3, and 50.2 mg kg-1, respectively, implying a significant accumulation of Cr, Ni, and Pb compared with the background values. Contamination factor indicates that the soil underwent none to medium pollution by Cu and Zn, medium to strong by Cr, none to strong by Pb, and medium pollution by Ni while the pollution load index means that the soil was subjected to intermediate contamination. Based on the critical threshold values to protect the plants, the investigated metals were unable to affect the plants. One-way ANOVA analysis shows that Cu, Zn, and Pb in plants varied with plant tissues. Cu-Cr, Cu-Ni, Zn-Ni, Zn-Pb, Cr-Ni, and Ni-Pb pairs had significant positive correlation both in soil and in plants due to the similar soil characteristics and plant physiologies. Correspondence analysis indicates that Pb was more likely to be accumulative in stems and leaves. In addition, the levels of Cu and Cr in plant followed an order of roots > stems > leaves; Zn and Ni leaves ≥ stems > roots; and Pb followed stems ≥ leaves > roots. Generally, this study suggests that the plants like Ligustrum lucidum Aiton and Weigela hortensis, which are capable of accumulating Cr, Ni, and Pb, should be the predominant species in the studied area.

Potential impacts of coal mining activities on nitrate sources and transport in a karst river basin in southwest China.

Typical sources of nitrate pollution in the fragile ecological environment of karst areas, such as agricultural production activities and domestic sewage, have long attracted serious concern. However, coal development can play an equally significant role in releasing the nitrogen fixed in coal into surface watersheds in the form of nitrate, nitrite, or ammonia, consequently threatening the water quality of surface water systems in mining areas. In this study, a typical karst surface watershed system affected by coal mining activities was selected for an in-depth investigation with the aim of realistically assessing the potential contribution of coal mining to nitrogen pollution. The results reveal increasingly concerning nitrate pollution from August 2020 to November 2021 in the Huatan River watershed under the influence of anthropogenic activities, especially mining development and agricultural production. Given that the nitrogen and oxygen isotope compositions of nitrate do not support the presence of denitrification, the variation in the NO3-/Cl- ratio and the relatively stable Cl- concentration may be a reflection of nitrification. Although the leaching of atmospheric precipitation on the strata in the basin promoted the release of nitrogen associated with coal mining, the higher rate of nitrogen cycling in the oligotrophic mine water environment limited the contribution of coal mining to nitrogen pollution in the surface watershed. Specifically, the contribution of coal mining activities to nitrogen pollution in surface karst river is mainly NH4+-N, which contributes 10% or less to the nitrate input to the waters of the Huatan River. The findings thus highlight the necessity of further uncovering the geochemical cycling process of nitrogen during the transport of mine water in the coal mining environment.

Arbuscular mycorrhizal fungal inoculum and N2-fixing plants in ecological reclamation of arid mining areas: nutrient limitation of the moss biocrust microbiome.

Ecoenzymatic stoichiometry can reflect the ability of soil microorganisms to acquire energy and nutrients and to determine their response to environmental stresses. However, the drivers of metabolic limitation of the moss biocrust microbiome during the ecological restoration of coal mining areas are poorly understood. Therefore, in this study, enzymatic stoichiometry modeling and high-throughput sequencing were used to simultaneously determine moss biocrust microbial metabolic limitation and its relationship with moss biocrust nutrients and arbuscular mycorrhizal fungal (AMF) diversity in five arid and semi-arid revegetation types (Hippophae rhamnoides, Amorpha fruticosa, Cerasus humilis, Cerasus szechuanica, and Xanthoceras sorbifolium) and two microbial treatments (AMF-inoculated and uninoculated). The activities of moss biocrust carbon (C)-, nitrogen (N)-, and phosphorus (P)-acquiring enzymes and organic carbon fractions in the AMF-inoculated treatment were significantly higher than those in the uninoculated control. Moss biocrust microbial community C and P limitations were observed in the five revegetation types, with lower limitation in general in the AMF-inoculated treatment. Dinitrogen-fixing plants (Amorpha fruticosa and Hippophae rhamnoides) significantly mitigated moss biocrust microbiome C and P limitation, especially in the AMF-inoculated treatment. Furthermore, partial least squares path modeling (PLS-PM) shows that moss biocrust organic carbon fractions (- 0.73 and - 0.81 of the total effects, respectively) and AMF diversity (- 0.73 and - 0.81 of the total effects) had negative effect on microbial C and P limitation, suggesting that more efficient active nutrients and AMF diversity are important factors alleviating limitation of moss biocrust microbial metabolism. This indicates that moss biocrust microbial communities under N2-fixing species with AMF inoculation were more stable under environmental stress; thus, AMF inoculation and/or N2-fixing plants may be recommended as preferred options for the ecological restoration of arid mining areas.

Pseudomonas fluorescens with Nitrogen-Fixing Function Facilitates Nitrogen Recovery in Reclaimed Coal Mining Soils.

Coal mining has caused significant soil nitrogen loss in mining areas, limiting reclamation and reuse in agriculture. This article studies the effects of organic fertilizer, inorganic fertilizer, and the combined application of Pseudomonas fluorescens with the ability of nitrogen fixation on soil nitrogen accumulation and composition in the reclamation area of the Tunlan Coal Mine from 2016 to 2022 under the conditions of equal nitrogen application, providing a scientific basis for microbial fertilization and the rapid increase in nitrogen content in the reclaimed soil of mining areas. The results showed that as the reclamation time increased, the nitrogen content and the composition and structure of the soil treated with fertilization rapidly evolved toward normal farmland soil. The soil nitrogen content increased most rapidly in the presence of added P. fluorescens + organic fertilizer (MB). Compared to other treatments (inorganic fertilizer (CF), organic fertilizer (M), and P. fluorescens + inorganic fertilizer (CFB)), MB increased total nitrogen (TN) to normal farmland soil levels 1-3 years earlier. The comprehensive scores of MB and CFB on the two principal components increased by 1.58 and 0.79 compared to those of M and CF treatments, respectively. This indicates that the combination of P. fluorescens and organic fertilizer improves soil nitrogen accumulation more effectively than the combination of P. fluorescens and inorganic fertilizer. In addition, the application of P. fluorescens increases the content of unknown nitrogen (UN) in acid-hydrolysable nitrogen (AHN) and decreases the content of amino acid nitrogen (AAN) and ammonia nitrogen (AN). However, there was no significant effect on the content of ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3--N) in soil-mineralized nitrogen (SMN). When combined with inorganic fertilizer, the contribution of SMN to TN increased by 14.78%, while when combined with organic fertilizer, the contribution of AHN to TN increased by 44.77%. In summary, the use of P. fluorescens is beneficial for nitrogen recovery in the reclaimed soil of coal-mining areas. The optimal fertilization method under the experimental conditions is the combination of P. fluorescens and organic fertilizer.

Radiological Analysis of Cassava Samples From a Coal Mining Area in Enugu State Nigeria.

Cassava holds a vital position as a staple food in Nigeria, forming a significant portion of the daily diet for the population. Unfortunately, food intake can serve as a pathway for radiological contamination in humans and animals. In this study conducted in an old coal mining area in Enugu State, Nigeria, cassava samples from the area were analyzed using gamma ray spectroscopy. The results revealed significant mean activity concentrations of the radionuclides 40K, 226Ra, and 232Th in camp 1, camp 2, and Pottery areas. The activity concentration ranged from 193.68 to 300.92 Bq/kg for 40 K, 23.03 to 37.24 Bq/kg for 226Ra, and 135.33 to 158.43 Bq/kg for 232Th, respectively. Of concern is the total mean annual effective dose resulting from exposure to these 3 observed radionuclides that was calculated to be 2.03 mSv/yr. This value exceeds the recommended limit of 1 mSv/yr, indicating potential health risks associated with the radiological contamination from cassava consumption in this region. In summary, the study shows that cassava samples from the investigated area exhibited elevated levels of radiotoxicity, raising concerns about the safety of consuming cassava from this region as a food source.

Water chemistry and stable isotope characteristics of subsidence lakes in coal mining areas, Eastern China.

Many subsidence lakes have formed in eastern China as a result of underground coal mining. These coal mining-related subsidence lakes vary in their formation time and connectivity with rivers. These factors may influence the water chemistry and hydrogen and oxygen stable isotope characteristics of the lake water. This study collected and tested subsidence lake water, atmospheric precipitation, river water, and shallow groundwater in the study area. The results showed that the water chemical types of the subsidence lake water and river water are Cl-Na and HCO3·Cl-Na and that the water chemical types of the shallow groundwater are mainly HCO3·Cl-Na and HCO3·Cl-Ca. There are no significant differences in the water chemical characteristics of subsidence lakes with different subsidence ages and types. The major ions in each water body mainly come from evaporite dissolution and silicate weathering, and ion exchange occurs. Reverse ion exchange occurs in some shallow groundwater samples. The stable isotopes of hydrogen and oxygen in the subsidence lake water, river water, and shallow groundwater are distributed along a straight line with a slope less than that of the LMWL, indicating that these water bodies have a common source, namely, precipitation. With increases in the formation time of the subsidence lakes, the heavy isotopes in the lake water gradually become depleted, and the d value gradually increases, mainly driven by precipitation dilution, weakening evaporation, river recharge, and groundwater recharge. The isotopic values of different types of lakes with the same subsidence time differ little. The research results may provide scientific guidance for the rational development and utilization of water resources in coal mining subsidence areas, enrich the study of the hydrological cycle in the area, and are of great significance for the protection of the local water balance and water environment.

Health risk assessment from exposure to ambient VOCs and particulate matter in different functional zones in Dhanbad, India.

This study evaluates the non-cancer risks (NCR) and cancer risks (CR) in outdoor working personnel in Dhanbad city, exposed to volatile organic compounds (VOCs) and particulate matter (PM) present in the ambient air. Dhanbad is known for its coal mines and is one of the most polluted cities in India and the world. Sampling was conducted in different functional zones; namely, traffic intersections, industrial, and institutional areas, to estimate the concentration of different PM-bound heavy metals and VOCs in the ambient air using Inductively coupled plasma-optical emission spectrometry (ICP-OES) and gas chromatography (GC) respectively. Our results show that the concentration levels (of VOCs and PM) and health risks were maximum at the traffic intersection area, followed by the industrial and institutional areas. The major contribution to CR came from chloroform, naphthalene, and PM-bound chromium, while the contribution to NCR mainly came from naphthalene, trichloroethylene, xylenes, and PM-bound chromium, nickel, and cadmium. It was observed that CR and NCR from VOCs are quite comparable (average CRvoc 8.92E-05 and NCRvoc 6.82) to that from the PM-bound heavy metals (average CRPM 9.93E-05 and NCRPM 3.52). According to the sensitivity analysis performed using Monte-Carlo Simulation, it was also found that the output risk is most significantly influenced by the pollutant concentration followed by exposure duration and exposure time. The study reveals that Dhanbad city is not only a critically polluted area but also a highly hazardous and cancer-prone area due to the pollution arising from incessant coal mining activities and heavy vehicular movement. Given the paucity of data on exposure to VOCs in ambient air and their risk assessment pertaining to coal mining cities of India, our study provides useful information and insight for the regulatory and enforcing authorities to devise appropriate strategies for air pollution and health risk management in such cities.

[System Construction and the Function Improvement of Ecological Carbon Sink in Coal Mining Areas Under the Carbon Neutral Strategy].

The loss of ecological carbon sinks often occurs in the process of coal resource development. Under the carbon neutral strategy, it is of great significance to explore technologies and models for improving ecological carbon sinks in coal mining areas. This study firstly addressed the system construction framework of the ecological carbon sink in coal mining areas, which included two levels of management mode and technical methods; three main categories of soil carbon sink, vegetation carbon sink, and wetland carbon sink; and several technical contents such as ecological carbon sink planning, carbon sink monitoring and investigation, carbon sink function improvement, and carbon sink loss prevention. The study analyzed the main types of ecological carbon sink (mainly involving soil carbon sinks and vegetation carbon sinks, whereas wetland carbon sinks were mainly related to coal mining subsidence areas with high groundwater level) and circumstances of carbon sink losses (including coal mining activities, the process of ecological vegetation construction, and ecological stability risk under long-term conditions) and proposed methods to improve ecological carbon sinks and prevent carbon sink losses for soil carbon sinks and vegetation carbon sinks in coal mining areas. The results can provide technical reference for the scientific research and engineering construction of ecological carbon sinks in coal mining areas.

[Effects of biochar and biochar compound fertilizer on the communities of nitrifier and denitrifier in a reclaimed soil from coal-mining area]. / ç”Ÿç‰©ç‚­ä¸Žç‚­åŸºè‚¥å¯¹é‡‡ç ¤å¡Œé™·å¤åž¦åŒºåœŸå£¤ç¡åŒ–å’Œåç¡åŒ–å¾®ç”Ÿç‰©ç¾¤è½çš„å½±å“.

As a new soil amendment strategy, the effects of biochar application on soil microbial community have been reported in literature, but little information is available on the response of nitrogen-related microbial communities to biochar application in the reclaimed soil from coal-mining area. Through an outdoor pot experiment with reclaimed soil from coal-mining area of Huaibei, the effects of biochar and biochar compound fertilizer on nitrifier and denitrifier communities were investigated by real time PCR (qPCR) and terminal-restricted fragment length polymorphism (T-RFLP). There were five treatments: control (CK), NPK fertilizer (CF), biochar compound fertili-zer (BF), 2% biochar and NPK fertilizer (LB), 4% biochar and NPK fertilizer (HB). Results showed that compared with the CK, the treatments CF, BF, LB and HB significantly increased the abundance of ammonia oxidizing archaea (AOA), ammonia oxidizing bacteria (AOB), nirK and nirS genes of denitrifier. Compared with the CF treatment, BF, LB and HB significantly increased the abundance of AOB and nirK genes by 42.9%-82.1% and 33.5%-62.7%, respectively. Results of redundancy analysis showed that soil organic carbon, pH, NH4+-N and available potassium significantly affected AOB community structure, while soil organic carbon concentration, pH and NO3--N concentration significantly altered nirK-denitrifier community structure. Therefore, the application of biochar and biochar compound fertilizer could improve soil quality of the reclaimed soil from coal-mining area, and increase the abundances of nitrifier and denitrifier and alter community structure of AOB and nirK-denitrifier.

Effect of coal mining activities and related industry on composition, cytotoxicity and genotoxicity of surrounding soils.

Coal mining and related industries each leave their characteristic "metal fingerprint" in the surrounding soils. Although geochemical investigations of such soils most often indicate heavy contamination with certain metals and bioassays point to their cytotoxic and genotoxic effects, the majority of studies are based on only one of the mentioned approaches. Here, the presented study investigated the effect of coal mining activities and related industry on surrounding soils by means of both geochemical and biological tools. The multielement composition of soils and associated eluates were used for the assessment of soil contamination level and the element bioavailable fractions, respectively. For cytotoxicity and genotoxicity evaluation, shallot (Allium ascalonicum L.) roots were exposed to selected soil eluates. Root growth, frequency of mitosis, mitotic and chromosomal abnormalities in root meristem cells, level of lipid peroxidation, and DNA damage evaluated by a comet assay were scored as toxicity endpoints. The results point to significant differences in the composition of collected soils and a variety of factors that contribute not only to their total metal load but also to the observed cytotoxic and genotoxic effects; all of which emphasize the necessity of a multidisciplinary approach in assessing the impact of anthropogenic activities on the environment, especially in historical mining areas.

Tracking the sources of allochthonous organic matter along a subtropical fluvial-estuarine gradient using molecular proxies in view of land uses.

Sedimentary sterols and linear alkylbenzenes associated with allochthonous organic matter (AOM) inputs were studied in surface sediments along the Tubarão riverbed, South Brazil. These markers were analysed in terms of concentrations, diagnostic ratios and by using multivariate analyses to identify the main organic matter sources. It was necessary to integrate all these factors to distinguish the sources and determine sewage contamination. Phytosterols predominated over faecal sterols, but the contributions of livestock waste along the river (determined in 50% of the sites) were confirmed by the fingerprint analysis. Raw sewage contamination was verified at one site, according to the increased levels of sewage molecular markers and confirmed by the multivariate analyses and diagnostic ratios calibrated to this region. A possible synergistic effect between inorganic nanoparticles from coal mine waste and organic contaminants related to AOM input was suggested and should not be ignored since both activities severely contribute to the environmental changes in much of this fluvial-estuarine gradient from the South Atlantic.

[Effects of biochar application on the abundance and structure of ammonia-oxidizer communities in coal-mining area.] / æ–½ç”¨ç”Ÿç‰©è´¨ç‚­å¯¹é‡‡ç ¤å¡Œé™·åŒºåœŸå£¤æ°¨æ°§åŒ–å¾®ç”Ÿç‰©ä¸°åº¦å’Œç¾¤è½ç»“æž„çš„å½±å“.

As a new type of soil amendment, biochar can effectively improve soil fertility, structure and soil nitrogen transformation. We studied the effects of biochar application on soil properties, abundance and community structure of ammonia oxidizer in coal-mining area. The results showed that the biochar application significantly increased contents of soil NH4+-N, total nitrogen, available phosphorus and potassium. Compared with the control, no change in the abundance of ammonia-oxidizing archaea (AOA) was found under biochar treatment, but there was a significant increase in the abundance of ammonia-oxidizing bacteria (AOB). The analysis of T-RFLP profiles showed that biochar significantly increased the diversity indexes of AOA and AOB, and altered the community structure of both AOA and AOB. Improved soil nutrients as well as increased abundance and diversity of ammonia-oxidizing community to some extent indicated the potential of biochar application in reclamation of coal-mining area soil.