Mechanisms Limiting Renal Tissue Protection and Repair in Glomerulonephritis.

Glomerulonephritis are renal disorders resulting from different pathogenic mechanisms (i.e., autoimmunity, complement, inflammatory activation, etc.). Clarifying details of the pathogenic cascade is basic to limit the progression from starting inflammation to degenerative stages. The balance between tissue injury, activation of protective systems and renal tissue repair determines the final outcome. Induction of an oxidative stress is part of glomerular inflammation and activation of protective antioxidant systems has a crucial role in reducing tissue effects. The generation of highly reactive oxygen species can be evaluated in vivo by tracing the inner-layer content of phosphatidyl ethanolamine and phosphatidyl serine in cell membranes. Albumin is the major antioxidant in serum and the level of oxidized albumin is another indirect sign of oxidative stress. Studies performed in Gn, specifically in FSGS, showed a high degree of oxidation in most contexts. High levels of circulating anti-SOD2 antibodies, limiting the detoxyfing activity of SOD2, have been detected in autoimmune Gn(lupus nephritis and membranous nephropathy) in association with persistence of proteinuria and worsening of renal function. In renal transplant, high levels of circulating anti-Glutathione S-transferase antibodies have been correlated with chronic antibody rejection and progressive loss of renal function. Annexins, mainly ANXA1 and ANXA2, play a general anti-inflammatory effect by inhibiting neutrophil functions. Cytosolic ANXA1 is decreased in apoptotic neutrophils of patients with glomerular polyangitis in association with delayed apoptosis that is considered the mechanism for polyangitis. High circulating levels of anti-ANXA1 and anti-ANXA2 antibodies characterize lupus nephritis implying a reduced anti-inflammatory effect. High circulating levels of antibodies targeting Macrophages (anti-FMNL1) have been detected in Gn in association with proteinuria. They potentially modify the intra-glomerular presence of protective macrophages (M2a, M2c) thus acting on the composition of renal infiltrate and on tissue repair.

Novel biomarkers and pathophysiology of membranous nephropathy: PLA2R and beyond.

Research on membranous nephropathy truly exploded in the last 15 years. This happened because of the application of new techniques (laser capture microdissection, mass spectrometry, protein G immunoprecipitation, arrays) to the study of its pathogenesis. After the discovery of PLA2R as the major target antigen, many other antigens were identified and others are probably ongoing. Clinical and pathophysiology rebounds of new discoveries are relevant in terms of diagnosis and prognosis and it is time to make a first assessment of the innovative issues. In terms of classification, target antigens can be divided into: 'membrane antigens' and 'second wave' antigens. The first group consists of antigens constitutionally expressed on the podocyte membrane (as PLA2R) that may become a target of an autoimmune process because of perturbation of immune-tolerance. 'Second wave' antigens are antigens neo-expressed by the podocyte or by infiltrating cells after a stressing event: this allows the immune system to produce antibodies against them that intensify and maintain glomerular damage. With this abundance of target antigens it is not possible, at the moment, to test all antibodies at the bedside. In the absence of this possibility, the role of histological evaluation is still irreplaceable.

Comparative studies of transformation behaviors and mechanisms of halophenols in multiple chemical oxidative systems.

Due to wide applications, halophenols (HPs), especially bromophenols, chlorophenols, and fluorophenols, are commonly detected but resistant to biological removal in wastewater treatment plants (WWTPs). This study investigated the overall transformation behaviors of three representative HPs (2,4-dichlorophenol: 24-DCP, 2,4-dibromophenol: 24-DBP, 2,4-difluorophenol: 24-DFP) in six chemical oxidative systems (KMnO4, K2FeO4, NaClO, O3, UV, and persulfate (PS)). The results revealed fast removal of selected HPs by O3, PS and K2FeO4, while a large discrepancy in their removal efficiencies occurred under UV irradiation, KMnO4 oxidation and particularly chlorination. Based on the analysis of the identified intermediates and products, coupling among the five routes was the general route, and dimers were the main intermediates for HP oxidation. The effect of the halogen atom on the transformation pathways of HPs was highly reaction type dependent. Among the six chemical treatments, PS could induce HPs to yield relatively low-molecular-weight polymers and obtain the highest coupling degree. Transition state (TS) calculations showed that the H atom linked to the phenoxy group of HPs was the most easily abstracted by hydroxyl radicals to form the coupling precursor, i.e., phenoxy radicals. This high coupling behavior further resulted in the increased toxicity to green algae. Characterization revealed that HP reaction solutions treated with PS had a severely negative effect on algae growth, photosynthetic pigment synthesis, and the antioxidant enzyme system. These findings can shed light on the reaction mechanisms of advanced oxidation technologies and some risk management and control of PS technique may be considered when treating phenolic pollutants.

Occurrence, statutory guideline values and removal of contaminants of emerging concern by Electrochemical Advanced Oxidation Processes: A review.

A wide variety of chemical compounds are used in human activities; however, part of these compounds reach surface water, groundwater and even water considered for potable uses. Due to the limited efficiency of water treatment by the Water and Wastewater Treatment Plants, the presence of these compounds in natural and human consumption waters can be very harmful due to their high persistence and adverse effects; these characteristics define the contaminants of emerging concern (CECs). Water treatment by Electrochemical Advanced Oxidation Processes (EAOPs) has been evaluated as a promising process for the removal of persistent and recalcitrant organic contaminants. With this background, the present review aims to gather studies and information published between 2015 and 2020 regarding the occurrence of CECs in surface, potable and groundwater, its treatment by EAOPs, the main operating conditions and by-product generation of EAOPs, contaminant toxicity assessments and international statutory guideline values concerning CEC standards and allowable concentrations in the environment and treated drinking water. Therefore, in this review it was found that the compounds bisphenol A (BPA), diethyltoluamide (DEET), 17α-ethinyl estradiol (EE2), perfluorobutanoic acid (PFBA), carbamazepine, caffeine and atrazine were the most frequently detected in water sources, with concentrations ranging from 35.54-4800, 1.21-98, 0.005-38.5, 5-742.904, 0.0071-586, 0.89-1040, and 100-323 (ng L-1), respectively. Among the operational conditions of EAOPs, current density, pH and oxidant concentration are the main operational parameters that have an influence on these treatment technologies, besides the by-products generated, which might be removed by the integration of EAOPs with biological digestion treatments. Regarding the values of water quality standards, many CECs do not have established standard allowable concentration values, which represents a concern toward the possible toxic effects of these compounds on non-target organisms.

Study of Hydrogen Peroxide as a Senescence-Inducing Signal.

In many plant species, leaf senescence correlates with an increase in intracellular levels of reactive oxygen species (ROS) as well as differential regulation of anti-oxidative systems. Due to their reactive nature, reactive oxygen species (ROS) were considered to have only detrimental effects for long time. However, ROS turned out to be more than just toxic by-products of aerobic metabolism but rather major components in different signaling pathways. Considering its relatively long half-life, comparably low reactivity, and its ability to cross membranes, especially hydrogen peroxide, has gained attention as a signaling molecule. In this article, a set of tools to study hydrogen peroxide contents and the activity of its scavenging enzymes in correlation with leaf senescence parameters is presented.