The microbiome stabilizes circadian rhythms in the gut.

The gut microbiome is well known to impact host physiology and health. Given widespread control of physiology by circadian clocks, we asked how the microbiome interacts with circadian rhythms in the Drosophila gut. The microbiome did not cycle in flies fed ad libitum, and timed feeding (TF) drove limited cycling only in clockless per01 flies. However, TF and loss of the microbiome influenced the composition of the gut cycling transcriptome, independently and together. Moreover, both interventions increased the amplitude of rhythmic gene expression, with effects of TF at least partly due to changes in histone acetylation. Contrary to expectations, timed feeding rendered animals more sensitive to stress. Analysis of microbiome function in circadian physiology revealed that germ-free flies reset more rapidly with shifts in the light:dark cycle. We propose that the microbiome stabilizes cycling in the host gut to prevent rapid fluctuations with changing environmental conditions.

Thermal refugia reinforce macroalgal resilience against climate change in the southeastern Bay of Biscay.

Rising global temperatures present unprecedented challenges to marine ecosystems, demanding a profound understanding of their ecological dynamics for effective conservation strategies. Over a comprehensive macroalgal assessment spanning three decades, we investigated the spatiotemporal evolution of shallow-water benthic communities in the southern Bay of Biscay, uncovering climate-resilient areas amidst the ongoing phase shift in the region. Our investigation identified seven locations serving as potential climate refugia, where cold-affinity, canopy-forming macroalgal species persisted and community structure was similar to that observed in 1991. We unveiled a clear association between the emergence of these refugia, sea surface temperature (SST), and the Community Temperature Index, positioning SST as a significant driver of the observed phase shift in the region. Warming processes, defined as tropicalization (increase of warm-affinity species) and deborealization (decrease of cold-affinity species), were prominent outside refugia. In contrast, cooling processes, defined as borealization (increase of cold-affinity species) and detropicalization (decrease of warm-affinity species), prevailed inside refugia. Refugia exhibited approximately 35% lower warming processes compared to non-refuge areas. This resulted in a dominance of warm-affinity species outside refugia, contrasting with the stability observed within refugia. The persistence of canopy-forming species in refuge areas significantly contributed to maintaining ecosystem diversity and stability. These findings underscored the pivotal role of climate refugia in mitigating climate-driven impacts. Prioritizing the protection and restoration of these refugia can foster resilience and ensure the preservation of biodiversity for future generations. Our study illustrates the importance of refining our understanding of how marine ecosystems respond to climate change, offering actionable insights essential for informed conservation strategies and sustainable environmental management.

Joint Beamforming and Phase Shifts Design for RIS-Aided Multi-User Full-Duplex Systems in Smart Cities.

Full-duplex (FD) and reconfigurable intelligent surface (RIS) are potential technologies for achieving wireless communication effectively. Therefore, in theory, the RIS-aided FD system is supposed to enhance spectral efficiency significantly for the ubiquitous Internet of Things devices in smart cities. However, this technology additionally induces the loop-interference (LI) of RIS on the residual self-interference (SI) of the FD base station, especially in complicated urban outdoor environments, which will somewhat counterbalance the performance benefit. Inspired by this, we first establish an objective and constraints considering the residual SI and LI in two typical urban outdoor scenarios. Then, we decompose the original problem into two subproblems according to the variable types and jointly design the beamforming matrices and phase shifts vector methods. Specifically, we propose a successive convex approximation algorithm and a soft actor-critic deep reinforcement learning-related scheme to solve the subproblems alternately. To prove the effectiveness of our proposal, we introduce benchmarks of RIS phase shifts design for comparison. The simulation results show that the performance of the low-complexity proposed algorithm is only slightly lower than the exhaustive search method and outperforms the fixed-point iteration scheme. Moreover, the proposal in scenario two is more outstanding, demonstrating the application predominance in urban outdoor environments.

Contrasting Microbiome Dynamics of Putative Denitrifying Bacteria in Two Octocoral Species Exposed to Dissolved Organic Carbon (DOC) and Warming.

Mutualistic nutrient cycling in the coral-algae symbiosis depends on limited nitrogen (N) availability for algal symbionts. Denitrifying prokaryotes capable of reducing nitrate or nitrite to dinitrogen could thus support coral holobiont functioning by limiting N availability. Octocorals show some of the highest denitrification rates among reef organisms; however, little is known about the community structures of associated denitrifiers and their response to environmental fluctuations. Combining 16S rRNA gene amplicon sequencing with nirS in-silico PCR and quantitative PCR, we found differences in bacterial community dynamics between two octocorals exposed to excess dissolved organic carbon (DOC) and concomitant warming. Although bacterial communities of the gorgonian Pinnigorgia flava remained largely unaffected by DOC and warming, the soft coral Xenia umbellata exhibited a pronounced shift toward Alphaproteobacteria dominance under excess DOC. Likewise, the relative abundance of denitrifiers was not altered in P. flava but decreased by 1 order of magnitude in X. umbellata under excess DOC, likely due to decreased proportions of Ruegeria spp. Given that holobiont C:N ratios remained stable in P. flava but showed a pronounced increase with excess DOC in X. umbellata, our results suggest that microbial community dynamics may reflect the nutritional status of the holobiont. Hence, denitrifier abundance may be directly linked to N availability. This suggests a passive regulation of N cycling microbes based on N availability, which could help stabilize nutrient limitation in the coral-algal symbiosis and thereby support holobiont functioning in a changing environment. IMPORTANCE Octocorals are important members of reef-associated benthic communities that can rapidly replace scleractinian corals as the dominant ecosystem engineers on degraded reefs. Considering the substantial change in the (a)biotic environment that is commonly driving reef degradation, maintaining a dynamic and metabolically diverse microbial community might contribute to octocoral acclimatization. Nitrogen (N) cycling microbes, in particular denitrifying prokaryotes, may support holobiont functioning by limiting internal N availability, but little is known about the identity and (a)biotic drivers of octocoral-associated denitrifiers. Here, we show contrasting dynamics of bacterial communities associated with two common octocoral species, the soft coral Xenia umbellata and the gorgonian Pinnigorgia flava after a 6-week exposure to excess dissolved organic carbon under concomitant warming conditions. The specific responses of denitrifier communities of the two octocoral species aligned with the nutritional status of holobiont members. This suggests a passive regulation based on N availability in the coral holobiont.

Spatial covariation in nutrient enrichment and fishing of herbivores in an oceanic coral reef ecosystem.

Both natural and anthropogenic stressors are increasing on coral reefs, resulting in large-scale loss of coral and potential shifts from coral- to macroalgae-dominated community states. Two factors implicated in shifts to macroalgae are nutrient enrichment and fishing of reef herbivores. Although either of these factors alone could facilitate establishment of macroalgae, reefs may be particularly vulnerable to coral-to-algae phase shifts in which strong bottom-up forcing from nutrient enrichment is accompanied by a weakening of herbivore control of macroalgae via intense fishing. We explored spatial heterogeneity and covariance in these drivers on reefs in the lagoons of Moorea, French Polynesia, where the local fishery heavily targets herbivorous fishes and there are spatially variable inputs of nutrients from agricultural fertilizers and wastewater systems. Spatial patterns of fishing and nutrient enrichment were not correlated at the two landscape scales we examined: among the 11 interconnected lagoons around the island or among major habitats (fringing reef, mid-lagoon, back reef) within a lagoon. This decoupling at the landscape scale resulted from patterns of covariation between enrichment and fishing that differed qualitatively between cross-shore and long-shore directions. At the cross-shore scale, nutrient enrichment declined but fishing increased from shore to the crest of the barrier reef. By contrast, nutrient enrichment and fishing were positively correlated in the long-shore direction, with both increasing with proximity to a pass in the barrier reef. Contrary to widespread assumptions in the scientific literature that human coastal population density correlates with impact on marine ecosystems and that fishing effort declines linearly with distance from the shore, these local stressors produced a complex spatial mosaic of reef vulnerabilities. Our findings support spatially explicit management involving the control of anthropogenic nutrients and strategic reductions in fishing pressure on herbivores by highlighting specific areas to target for management actions.

On the force field optimisation of [Formula: see text]-lactam cores using the force field Toolkit.

When employing molecular dynamics (MD) simulations for computer-aided drug design, the quality of the used force fields is highly important. Here we present reparametrisations of the force fields for the core molecules from 9 different [Formula: see text]-lactam classes, for which we utilized the force field Toolkit and Gaussian calculations. We focus on the parametrisation of the dihedral angles, with the goal of reproducing the optimised quantum geometry in MD simulations. Parameters taken from CGenFF turn out to be a good initial guess for the multiplicity of each dihedral angle, but the key to a successful parametrisation is found to lie in the phase shifts. Based on the optimised quantum geometry, we come up with a strategy for predicting the phase shifts prior to the dihedral potential fitting. This allows us to successfully parameterise 8 out of the 11 molecules studied here, while the remaining 3 molecules can also be parameterised with small adjustments. Our work highlights the importance of predicting the dihedral phase shifts in the ligand parametrisation protocol, and provides a simple yet valuable strategy for improving the process of parameterising force fields of drug-like molecules.

Timeless Plays an Important Role in Compound Eye-Dependent Photic Entrainment of the Circadian Rhythm in the Cricket Gryllus bimaculatus.

The light cycle is the most powerful Zeitgeber entraining the circadian clock in most organisms. Insects use CRYPTOCHROMEs (CRYs) and/or the compound eye for the light perception necessary for photic entrainment. The molecular mechanism underlying CRY-dependent entrainment is well understood, while that of the compound eye-dependent entrainment remains to be elucidated. Using molecular and behavioral experiments, we investigated the role of timeless (tim) in the photic entrainment mechanism in the cricket Gryllus bimaculatus. RNA interference of tim (timRNAi) disrupted the entrainment or prolonged the transients for resynchronization to phase-delayed light-dark cycles. The treatment reduced the magnitude of phase delay caused by delayed light-off, but augmented advance shifts caused by light exposure at late night. TIM protein levels showed daily cycling with an increase during the night and reduction by light exposure at both early and late night. These results suggest that tim plays a critical role in the entrainment to delayed light cycles.

Dosimetric impact of phase shifts on Radixact Synchrony tracking system with patient-specific breathing patterns.

PURPOSE: The Synchrony tracking system of Radixact is capable of real-time tumor tracking by building a correlation model between external light-emitting diodes on the patient's chest and an internal marker. A phase shift between the chest wall and a lung tumor has been reported. Hence, this study focused on evaluating the accuracy of the tracking system, especially under a patient-specific breathing pattern with respiratory phase shifts. METHODS: A phantom containing fiducial markers was placed on a moving platform. The intrinsic delivery accuracy was verified with a patient-specific breathing pattern. Three patient-specific breathing patterns were then implemented, for which phase shifts, φ, were introduced. Phase shifts with +0.3 s and +1 s were tested for dosimetric aspects, whereas ±0.3, ±0.6, and ±0.8 s shifts were used for tracking accuracy. The resultant dose distributions were analyzed by γ comparison. Dose profiles in the superior-inferior and lateral directions were compared. Logfiles of the tracking information were extracted from the system and compared with the input breathing pattern. The root mean square (RMS) difference was used to quantify the consistency. RESULTS: When the φ value was as large as 1 s, a severe inconsistency was observed. The target was significantly underdosed, down to 89% of the originally planned dose. γ analysis revealed that the failed portion was concentrated in the target region. The RMS of the tracking difference was close to 1 mm when φ was ±0.3 s and approximately 4 mm when φ was ±0.8 s. Tracking errors increased with an increase in the degree of phase shifts. CONCLUSION: Phase shifts between the patient chest wall and the internal target may hamper treatment delivery and jeopardize treatment using Synchrony Tracking. Hence, a larger planning target volume (PTV) may be necessary if a large phase shift is observed in a patient, especially when an external surrogate shows a lag in motion when compared with the tumor.

Circadian Disruption and Consequences on Innate Immunity and Inflammatory Response.

Circadian rhythms control almost all aspects of physiology and behavior, allowing temporal synchrony of these processes between each other, as well as with the external environment. In the immune system, daily rhythms of leukocyte functions can determine the strength of the immune response, thereby regulating the efficiency of defense mechanisms to cope with infections or tissue injury. The natural light/dark cycle is the prominent synchronizing agent perceived by the circadian clock, but this role of light is highly compromised by irregular working schedules and unintentional exposure to artificial light at night (ALAN). The primary concern is disrupted circadian control of important physiological processes, underlying potential links to adverse health effects. Here, we first discuss the immune consequences of genetic circadian disruption induced by mutation or deletion of specific clock genes. Next, we evaluate experimental research into the effects of disruptive light/dark regimes, particularly light-phase shifts, dim ALAN, and constant light on the innate immune mechanisms under steady state and acute inflammation, and in the pathogenesis of common lifestyle diseases. We suggest that a better understanding of the mechanisms by which circadian disruption influences immune status can be of importance in the search for strategies to minimize the negative consequences of chronodisruption on health.

Trophic cascade in a marine protected area with artificial reefs: spiny lobster predation mitigates urchin barrens.

An ultimate benefit of marine protected areas (MPAs) is to reverse trophic cascades caused by human-driven collapse of critical ecological interactions. Here we demonstrate that, despite a small scale (0.28 km2 ) and not being fully protected, an MPA with strict fishing management and habitat enhancement by artificial reefs (ARs) in southwest Japan can lead to well-established macroalgal communities on widespread sea urchin barrens through cascading effects of predator recovery. Areas with low urchin densities occurred in and around daytime lobster (Panulirus japonicus) shelters primarily formed by quarry-rock ARs inside the MPA. We confirmed in the laboratory that lobsters preyed on two dominant sea urchins (Echinometra sp. A and Heliocidaris crassispina), with size- and species-dependent predation. The area with few urchins extended farther (˜65 m) from an AR with numerous lobsters than from a natural shelter (patch reef) with far fewer lobsters. Causation of this pattern was confirmed by a tethering experiment showing that predation on urchins was similarly high at and near lobster shelters but decreased at ˜100 m from the AR to a similar level as at an unprotected site. Time-lapse photography revealed that predation on tethered urchins was due mostly to the largest size class of lobsters (>100 mm carapace length), which comprised only 7% of the population, highlighting the importance of large-sized lobsters in controlling urchin abundance in localized areas adjacent to urchin-dominated barrens. Despite an ongoing once-a-year fishing event permitted within the MPA, lobster populations were persistent, demonstrating that the cascading effect of the lobsters on urchins and ultimately macroalgae was robust to temporary reductions in predator population size. Erect macroalgal cover was not simply accounted for by snapshot urchin density or biomass, suggesting a hysteresis effect of the phase shifts between macroalgal dominance and urchin barren states.

Ocean currents and herbivory drive macroalgae-to-coral community shift under climate warming.

Coral and macroalgal communities are threatened by global stressors. However, recently reported community shifts from temperate macroalgae to tropical corals offer conservation potential for corals at the expense of macroalgae under climate warming. Although such community shifts are expanding geographically, our understanding of the driving processes is still limited. Here, we reconstruct long-term climate-driven range shifts in 45 species of macroalgae, corals, and herbivorous fishes from over 60 years of records (mainly 1950-2015), stretching across 3,000 km of the Japanese archipelago from tropical to subarctic zones. Based on a revised coastal version of climate velocity trajectories, we found that prediction models combining the effects of climate and ocean currents consistently explained observed community shifts significantly better than those relying on climate alone. Corals and herbivorous fishes performed better at exploiting opportunities offered by this interaction. The contrasting range dynamics for these taxa suggest that ocean warming is promoting macroalgal-to-coral shifts both directly by increased competition from the expansion of tropical corals into the contracting temperate macroalgae, and indirectly via deforestation by the expansion of tropical herbivorous fish. Beyond individual species' effects, our results provide evidence on the important role that the interaction between climate warming and external forces conditioning the dispersal of organisms, such as ocean currents, can have in shaping community-level responses, with concomitant changes to ecosystem structure and functioning. Furthermore, we found that community shifts from macroalgae to corals might accelerate with future climate warming, highlighting the complexity of managing these evolving communities under future climate change.

Will coral reef sponges be winners in the Anthropocene?

Recent observations have shown that increases in climate change-related coral mortality cause changes in shallow coral reef community structure through phase shifts to alternative taxa. As a result, sponges have emerged as a potential candidate taxon to become a "winner," and therefore a numerically and functionally dominant member of many coral reef communities. But, in order for this to occur, there must be sufficient trophic resources to support larger populations of these active filter-feeding organisms. Globally, climate change is causing an increase in sea surface temperatures (SSTs) and a decrease in salinity, which can lead to an intensification in the stratification of shallow nearshore waters (0-200 m), that affects both the mixed layer depth (MLD) and the strength and duration of internal waves. Specifically, climate change-driven increases in SSTs for tropical waters are predicted to cause increased stratification, and more stabilized surface waters. This causes a shallowing of the MLD which prevents nutrients from reaching the euphotic zone, and is predicted to decrease net primary production (NPP) up to 20% by the end of the century. Lower NPP would subsequently affect multiple trophic levels, including shallow benthic filter-feeding communities, as the coupling between water column productivity and the benthos weakens. We argue here that sponge populations may actually be constrained, rather than promoted, by climate change due to decreases in their primary trophic resources, caused by bottom-up forcing, secondary to physical changes in the water column (i.e., stratification and changes in the MLD resulting in lower nutrients and NPP). As a result, we predict sponge-dominated tropical reefs will be rare, or short-lived, if they occur at all into the future in the Anthropocene.

Phase shifts in high-beta- and low-gamma-band local field potentials predict the focus of visual spatial attention.

The local field potential (LFP) contains rich information about activity in local neuronal populations. However, it has been challenging to establish direct links between LFP modulations and task-relevant behavior or cognitive processes, such as attention. We sought to determine whether LFP amplitude or phase modulations are predictive of the allocation of visual spatial attention. LFPs were recorded simultaneously in multiple early visual brain structures of alert macaque monkeys performing attention-demanding detection and discrimination tasks. Attention directed toward the receptive field of recorded neurons generated systematically larger phase shifts in high-beta- and low-gamma-frequency LFPs compared with LFP phase shifts on trials in which attention was directed away from the receptive field. This attention-mediated temporal advance corresponded to ~10 ms. LFP phase shifts also correlated with reaction times when monkeys were engaged in the tasks. Importantly, attentional modulation of LFP phase was consistent across monkeys, tasks, visual brain structures, and cortical layers. In contrast, attentional modulation of LFP amplitude varied across frequency bands, visual structures/layers, and tasks. Because LFP phase shifts were robust, consistent, and predictive of spatial attention, they could serve as a reliable marker for attention signals in the brain. NEW & NOTEWORTHY Local field potentials (LFPs) reflect the activity of spatially localized populations of neurons. Whether alterations in LFP activity are indicative of cognitive processes, such as attention, is unclear. We found that shifts in the phase of LFPs measured in multiple visual brain areas reliably predicted the focus of spatial attention. LFP phase shifts could therefore serve as a marker for behaviorally relevant attention signals in the brain.

A triage framework for managing novel, hybrid, and designed marine ecosystems.

The novel ecosystem (NE) concept has been discussed in terrestrial restoration ecology over the last 15 years but has not yet found much traction in the marine context. Against a background of unprecedented environmental change, managers of natural marine resources have portfolios full of altered systems for which restoration to a previous historical baseline may be impractical for ecological, social, or financial reasons. In these cases, the NE concept is useful for weighing options and emphasizes the risk of doing nothing by forcing questions regarding the value of novelty and how it can best be managed in the marine realm. Here, we explore how the concept fits marine ecosystems. We propose a scheme regarding how the NE concept could be used as a triage framework for use in marine environments within the context of a decision framework that explicitly considers changed ecosystems and whether restoration is the best or only option. We propose a conceptual diagram to show where marine NEs fit in the continuum of unaltered to shifted marine ecosystems. Overall, we suggest that the NE concept is of interest to marine ecologists and resource managers because it introduces a new vocabulary for considering marine systems that have been changed through human actions but have not shifted to an alternate stable state. Although it remains to be seen whether the concept of marine NEs leads to better conservation and restoration decisions, we posit that the concept may help inform management decisions in an era of unprecedented global marine change.

Chemically cued suppression of coral reef resilience: Where is the tipping point?

Coral reefs worldwide are shifting from high-diversity, coral-dominated communities to low-diversity systems dominated by seaweeds. This shift can impact essential recovery processes such as larval recruitment and ecosystem resilience. Recent evidence suggests that chemical cues from certain corals attract, and from certain seaweeds suppress, recruitment of juvenile fishes, with loss of coral cover and increases in seaweed cover creating negative feedbacks that prevent reef recovery and sustain seaweed dominance. Unfortunately, the level of seaweed increase and coral decline that creates this chemically cued tipping point remains unknown, depriving managers of data-based targets to prevent damaging feedbacks. We conducted flume and field assays that suggest juvenile fishes sense and respond to cues produced by low levels of seaweed cover. However, the herbivore species we tested was more tolerant of degraded reef cues than non-herbivores, possibly providing some degree of resilience if these fishes recruit, consume macroalgae, and diminish negative cues.

Efficacy of Morning Shorter Wavelength Lighting in the Visible (Blue) Range and Broad-Spectrum or Blue-Enriched Bright White Light in Regulating Sleep, Mood, and Fatigue in Traumatic Brain Injury: A Systematic Review.

Traumatic brain injury (TBI) profoundly affects sleep, mood, and fatigue, impeding daily functioning and recovery. This systematic review evaluates the efficacy of morning shorter wavelength lighting in the visible (blue) range and broad-spectrum or blue-enriched bright white light exposure in mitigating these challenges among TBI patients. Through electronic database searches up to May 2023, studies assessing sleep, circadian rhythm, sleepiness, mood, and fatigue outcomes in TBI patients exposed to morning shorter wavelength lighting in the visible (blue) range and broad-spectrum or blue-enriched bright white light were identified. Seven studies involving 309 participants met the inclusion criteria. Results indicated consistent advancement in sleep timing among individuals with mild TBI, alongside improvements in total sleep time, mood, and reduced sleepiness with both types of light exposure, particularly in mild TBI cases. Notably, two studies demonstrated alleviation of fatigue exclusively in severe TBI cases following light exposure. Despite promising findings, evidence remains limited, emphasizing the need for future research with standardized protocols to confirm the potential and optimize the benefits of light therapy for TBI recovery.

Extending weeknight sleep duration in late-sleeping adolescents using morning bright light on weekends: a 3-week maintenance study.

Study Objectives: Our sleep extension intervention in adolescents showed that gradually shifting weekday bedtime earlier plus one weekend of morning bright light advanced circadian phase and increased weeknight sleep duration. Here, we examine at-home maintenance of these changes. Methods: Fourteen adolescents (15.3-17.9 years; 7 female) completed a 7-week study. After usual sleep at home (2-week baseline), intervention participants (n = 8) gradually advanced weekday bedtime (1 hour earlier than baseline during week 3; 2 hours earlier in week 4) and received bright light (~6000 lux; 2.5 hours) on both mornings of the intervening weekend. During three maintenance weeks, intervention participants were instructed to maintain their school-day wake-up time on all days, keep their early week four bedtimes, except on weekends when they could go to bed up to 1 hour later, and get a 2.5-hour light box exposure within 5 minutes of waking on one morning (Saturday or Sunday) of both weekends at home. Control participants (n = 6) slept as usual at home and did not receive weekend bright light. Dim light melatonin onset (DLMO) was measured after the 2-week baseline, 2-week intervention, and 3-week maintenance in all participants. Actigraphic sleep-wake was collected throughout. Results: After the 2-week intervention, DLMOs advanced more compared to control (37.0 ±â€…40.0 minutes vs. -14.7 ±â€…16.6 minutes), weekday sleep duration increased by 69.7 ±â€…27.8 minutes and sleep onset was 103.7 ±â€…14.2 minutes earlier compared to baseline. After three maintenance weeks, intervention participants showed negligible DLMO delays (-4.9 ±â€…22.9 minutes); weekday fall-asleep times and sleep durations also remained stable. Conclusions: Early circadian phase and extended sleep can be maintained with at-home weekend bright light.

High-quality Cymbidium mannii genome and multifaceted regulation of crassulacean acid metabolism in epiphytes.

Epiphytes with crassulacean acid metabolism (CAM) photosynthesis are widespread among vascular plants, and repeated evolution of CAM photosynthesis is a key innovation for micro-ecosystem adaptation. However, we lack a complete understanding of the molecular regulation of CAM photosynthesis in epiphytes. Here, we report a high-quality chromosome-level genome assembly of a CAM epiphyte, Cymbidium mannii (Orchidaceae). The 2.88-Gb orchid genome with a contig N50 of 22.7 Mb and 27 192 annotated genes was organized into 20 pseudochromosomes, 82.8% of which consisted of repetitive elements. Recent expansions of long terminal repeat retrotransposon families have made a major contribution to the evolution of genome size in Cymbidium orchids. We reveal a holistic scenario of molecular regulation of metabolic physiology using high-resolution transcriptomics, proteomics, and metabolomics data collected across a CAM diel cycle. Patterns of rhythmically oscillating metabolites, especially CAM-related products, reveal circadian rhythmicity in metabolite accumulation in epiphytes. Genome-wide analysis of transcript and protein level regulation revealed phase shifts during the multifaceted regulation of circadian metabolism. Notably, we observed diurnal expression of several core CAM genes (especially ßCA and PPC) that may be involved in temporal fixation of carbon sources. Our study provides a valuable resource for investigating post-transcription and translation scenarios in C. mannii, an Orchidaceae model for understanding the evolution of innovative traits in epiphytes.

Influences of dynamic load phase shifts on the energetics and biomechanics of humans.

Using load-suspended backpacks to reduce vertical peak dynamic load exerted on humans can reduce metabolic costs. However, is it possible to further reduce metabolic cost by modulating dynamic load phase shift? If so, is anti-phase better than the others? In this study, we investigated the biomechanics, energetics and trunk response under phase shifts. Nine subjects wearing an active backpack with 19.4 kg loads walked on a treadmill at 5 km h-1 with four phase shift trials (T1-T4) and a load-locked trial (LK). Our results show that anti-phase trial (T3) assists ankle more and reduces the moment and gastrocnemius medialis activity, while T4 assists knee more and reduces the moment and rectus femoris activity. Due to the load injecting more mechanical energy into human in T3 and T4, the positive centre-of-mass work is significantly reduced. However, the gross metabolic rate is lowest in T4 and 4.43% lower than in T2, which may be because the activations of erector spinae and gluteus maximus are reduced in T4. In addition, T3 increases trunk extensor effort, which may weaken the metabolic advantage. This study provides guidance for improving assistance strategies and human-load interfaces and deepens the understanding of the energetics and biomechanics of human loaded walking.

Recent and rapid reef recovery around Koh Phangan Island, Gulf of Thailand, driven by plate-like hard corals.

Mass bleaching events and local anthropogenic influences have changed the benthic communities of many coral reefs with pronounced spatial differences that are linked to resilience patterns. The Gulf of Thailand is an under-investigated region with only few existing datasets containing long-term developments of coral reef communities using the same method at fixed sites. We thus analyzed benthic community data from seven reefs surrounding the island of Koh Phangan collected between 2014 and 2022. Findings revealed that the average live hard coral cover around Koh Phangan increased from 37% to 55% over the observation period, while turf algae cover decreased from 52% to 29%, indicating some recovery of local reefs. This corresponds to a mean increased rate of coral cover by 2.2% per year. The increase in live hard coral cover was mainly driven by plate-like corals, which quadrupled in proportion over the last decade from 7% to 28% while branching corals decreased in proportion from 9% to 2%. Furthermore, the hard coral genus richness increased, indicating an increased hard coral diversity. While in other reefs, increasing live hard coral cover is often attributed to fast-growing, branching coral species, considered more susceptible to bleaching and other disturbances, the reefs around Koh Phangan recovered mainly via growth of plate-like corals, particularly of the genus Montipora. Although plate-like morphologies are not necessarily more bleaching tolerant, they are important for supporting reef fish abundance and structural complexity on reefs, aiding reef recovery and sturdiness. Hence, our findings indicate that the intensity of local stressors around Kho Phangan allows reef recovery driven by some hard coral species.