Sero-prevalence and risk factor analysis of Theileria equi infection in equids from different agro-climatic zones of Punjab (India) by Indirect Immunofluorescence Antibody test.

In the Indian sub-continent, equine piroplasmosis (EP) is an imperative tick-borne disease of equids predominately caused by obligatory intra-erythrocytic protozoa Theileria equi. Present exploration is the first epidemiological survey report based on Indirect Immunofluorescence Antibody test (IFAT). A total of 120 equines (98 horses and 22 donkeys/mules) from five districts of two main agro-climatic zones of Punjab were screened. The overall prevalence was 58.33%, with western plane zone at higher infection risk for T. equi (74.50%, OR = 7.45, 95% CI = 62.24-85.76); followed by western zone (46.38%, OR = 4.63, 95% CI = 34-57.7). District wise highest and lowest prevalence was recorded in district Fazilka (80.76%) and Bathinda (35.29%) respectively. IFAT revealed elevated risk of infection of T. equi in the farms with inappropriate management structure. The risk factor connected with the type of host species had an odds ratio of 1.67 for donkeys/mules versus horses for T. equi infection. Followed by presence/absence of tick on animal. This study reveals the endemicity of EP in Punjab with prominence of various odds of infection, associated with latent infection of T. equi detectable by serological techniques as it was noticed that western plane zone and western zone are more prone to EP infection.

Prevalence of gastrointestinal parasitism in small ruminants in western zone of Punjab, India.

AIM: The aim of this study was to explore the prevalence of gastrointestinal parasitism in small ruminants in relation to various risk factors in the western zone of Punjab. MATERIALS AND METHODS: During the study, 603 fecal samples (391 of sheep and 212 of goats) were examined qualitatively by floatation and sedimentation techniques, and quantitatively by McMaster technique. RESULTS: Out of the 603 fecal (391 sheep and 212 goats) samples examined, 501 were found positive for endoparasitic infection with an overall prevalence of 83.08%, consisting of 85.16% and 79.24% in sheep and goats, respectively. Egg per gram in sheep was apparently more 1441.88±77.72 than goats 1168.57±78.31. The associated risk factors with the prevalence of gastrointestinal tract (GIT) parasites showed that females (85.97%) were significantly more susceptible than males (69.23%). Age wise the adults (>6 months) were significantly more prone to parasitic infection as compared to young ones (<6 months). Seasonal variation was recorded throughout the year and was significantly highest during monsoon (90.10%), followed by winter (83.84%) and summer (78.35%). CONCLUSION: The study revealed an overall prevalence of 83.08% of GIT parasitic infections in small ruminants constituting 85.16% in sheep and 79.24% in goats in the western zone of Punjab. The most relevant risk factors for the prevalence of gastrointestinal parasitism in ruminants were sex, age, and season.