1.
Nat Chem Biol
; 7(1): 5-6, 2011 Jan.
Artículo
en Inglés
| MEDLINE
| ID: mdl-21164511
Asunto(s)
Bioquímica , Proteína Sustrato Asociada a CrK/química , Proteína Oncogénica v-crk/química , Proteína Sustrato Asociada a CrK/genética , Proteína Sustrato Asociada a CrK/metabolismo , Isomerismo , Proteína Oncogénica v-crk/genética , Proteína Oncogénica v-crk/metabolismo , Unión Proteica , Transducción de Señal/genética , Transducción de Señal/fisiología , Dominios Homologos src/genética , Dominios Homologos src/fisiología
2.
Bioconjug Chem
; 19(3): 588-91, 2008 Mar.
Artículo
en Inglés
| MEDLINE
| ID: mdl-18275131
RESUMEN
Lanthanide-binding tags (LBTs) are small, genetically encoded, versatile protein fusion partners that selectively bind lanthanide ions with high affinity. The LBT motif features a strategically positioned tryptophan residue that sensitizes Tb3+ luminescence upon excitation at 280 nm. Herein, we describe the preparation of new LBT peptides that incorporate unnatural amino acids in place of tryptophan, and which sensitize both Tb3+ and Eu3+ luminescence at lower energies. We also report the semisynthesis of proteins tagged with these new LBTs using native chemical ligation. This expands the scope of LBTs and will enable their wider use in luminescence applications.