RESUMEN
Oxidative and nitrosative stresses can damage cellular membranes, disrupt mitochondrial function, alter gene expression, promote the apoptosis and necrosis of hepatocytes, and increase fibrosis in diverse acute and chronic liver diseases, including autoimmune hepatitis. The objectives of this review are to describe the mechanisms of oxidative and nitrosative stresses in inflammatory liver disease, indicate the pathogenic implications of these stresses in autoimmune hepatitis, and suggest investigational opportunities to develop interventions that counter them. The principal antioxidant defenses, including glutathione production, the activities of antioxidant enzymes, and the release of the nuclear factor erythroid 2-related factor 2, may be inadequate or suppressed by transforming growth factor beta. The generation of reactive oxygen species can intensify nitrosative stress, and this stress may not be adequately modulated by the thioredoxin-thioredoxin reductase system and induce post-translational modifications of proteins that further disrupt hepatocyte function. The unfolded protein response and autophagy may be unable to restore redox stability, meet metabolic demands, and maintain hepatocyte survival. Emerging interventions with highly selective site- and organelle-specific actions may improve outcomes, and they include inhibitors of nicotinamide adenine dinucleotide phosphate oxidase, nitric oxide synthase, and transforming growth factor beta. Pharmacological manipulation of nuclear transcription factors may favor expression of antioxidant genes, and stimulation of chaperone proteins within the endoplasmic reticulum and modulation of autophagy may prevent hepatic fibrosis and enhance cell survival. These interventions constitute investigational opportunities to improve the management of autoimmune hepatitis.
Asunto(s)
Hepatitis Autoinmune/inmunología , Hígado/inmunología , Factor de Transcripción NF-E2/inmunología , Estrés Oxidativo/inmunología , Factor de Crecimiento Transformador beta/inmunología , Antioxidantes/metabolismo , Autofagia/inmunología , Glutatión/inmunología , Glutatión/metabolismo , Hepatitis Autoinmune/metabolismo , Hepatocitos/inmunología , Hepatocitos/metabolismo , Humanos , Hígado/metabolismo , Mitocondrias Hepáticas/metabolismo , NADPH Oxidasas/antagonistas & inhibidores , Factor de Transcripción NF-E2/metabolismo , Óxido Nítrico Sintasa/antagonistas & inhibidores , Nitrosación , Procesamiento Proteico-Postraduccional/inmunología , Especies Reactivas de Oxígeno/inmunología , Especies Reactivas de Oxígeno/metabolismo , Reductasa de Tiorredoxina-Disulfuro/inmunología , Reductasa de Tiorredoxina-Disulfuro/metabolismo , Tiorredoxinas/inmunología , Tiorredoxinas/metabolismo , Factor de Crecimiento Transformador beta/antagonistas & inhibidores , Factor de Crecimiento Transformador beta/metabolismo , Respuesta de Proteína Desplegada/inmunologíaRESUMEN
The thioredoxin system plays a critical role in maintaining the cytoplasm redox state, participating in functions that are important to the cellular viability of fungi. Although functional and structural information on targets in human pathogenic fungi has been scarcely described in the literature, such studies are essential for in silico drug design and biotechnological applications. Therefore, the aims of the present study were to produce recombinant proteins of the thioredoxin system from Candida albicans and evaluate their possible use as prophylactic or alternative therapies against the most important pathogenic fungus associated with nosocomial infections. We focused on biochemical and structural analyses of recombinant thioredoxin reductase from C. albicans with His-tag (CaTrxR-His) for further biotechnology applications. Heterologous CaTrxR-His was efficiently expressed in the soluble fraction of the Escherichia coli lysate. CaTrxR-His was obtained with a high level of purity and presented specific enzymatic activity. Conformational changes of the protein were observed at different pHs and temperatures, with higher thermal stability at pH 8.0. The CaTrxR-His vaccine was shown to effectively induce high levels of CaTrxR-specific immunoglobulin G antibodies in Balb/c mice and reduce the renal fungal burden of experimental disseminated candidiasis in mice. These data may greatly impact future development strategies for vaccine and drug designs against C. albicans infection.
Asunto(s)
Candida albicans/enzimología , Reductasa de Tiorredoxina-Disulfuro/inmunología , Reductasa de Tiorredoxina-Disulfuro/metabolismo , Animales , Anticuerpos Antifúngicos/sangre , Antígenos Fúngicos/genética , Antígenos Fúngicos/metabolismo , Candida albicans/genética , Estabilidad de Enzimas , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Concentración de Iones de Hidrógeno , Inmunoglobulina G/sangre , Ratones Endogámicos BALB C , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Proteínas Recombinantes/metabolismo , Temperatura , Reductasa de Tiorredoxina-Disulfuro/genéticaRESUMEN
Vulvovaginal candidiasis (VVC) is characterized by an infection of the vulva and vagina, mainly caused by Candida albicans, a commensal microorganism that inhabits the vaginal, digestive, and respiratory mucosae. Vulvovaginal candidiasis affects approximately 75% of women, and 5% develop the recurrent form (RVVC). The aim of the present study was to evaluate whether neutrophils microbicidal response is triggered when activated with RVVC isolates caused by C. albicans. Our results showed that RVVC isolates induced neutrophil migration but significantly decrease the microbicidal activity of neutrophils, compared with VVC and ASS isolates. The microbicidal activity of neutrophils is highly dependent on the production of reactive oxygen species/reactive nitrogen species (ROS/RNS). However, this isolate induced detoxification of ROS/RNS produced by neutrophils, reflected by the high level of thiol groups and by the oxygen consumption. Therefore, RVVC isolates induced biochemical changes in the inflammatory response triggered by neutrophils, and these effects were mainly related to the detoxification of ROS/RNS through the thioredoxin reductase (TR), a key antioxidant enzyme in fungi. This might be one of the resistance mechanisms triggered by RVVC caused by C. albicans.
Asunto(s)
Candida albicans/inmunología , Proteínas Fúngicas/inmunología , Neutrófilos/inmunología , Reductasa de Tiorredoxina-Disulfuro/inmunología , Vagina/inmunología , Candida albicans/patogenicidad , Candidiasis Vulvovaginal/microbiología , Movimiento Celular , Citotoxicidad Inmunológica , Femenino , Proteínas Fúngicas/metabolismo , Humanos , Ácido Hipocloroso/metabolismo , Neutrófilos/microbiología , Cultivo Primario de Células , Recurrencia , Compuestos de Sulfhidrilo/inmunología , Compuestos de Sulfhidrilo/metabolismo , Reductasa de Tiorredoxina-Disulfuro/metabolismo , Vagina/microbiologíaRESUMEN
Antioxidant systems are fundamental components of host-parasite interactions, and often play a key role in parasite survival. Here, we report the cloning, heterologous expression, and characterization of a thioredoxin glutathione reductase (TGR) from Fasciola hepatica. The deduced polypeptide sequence of the cloned open reading frame (ORF) confirmed the experimental N-terminus previously determined for a native F. hepatica TGR showing thioredoxin reductase (TR) activity. The sequence revealed the presence of a fusion between a glutaredoxin (Grx) and a TR domain, similar to that previously reported in Schistosoma mansoni and Echinococcus granulosus. The F. hepatica TGR sequence included an additional redox active center (ACUG; U being selenocysteine) located at the C-terminus. The addition of a recombinant selenocysteine insertion sequence (SECIS) element in the Escherichia coli expression vector, or the substitution of the native selenocysteine by a cysteine, indicated the relevance of this unusual amino acid residue for the activity of F. hepatica TGR. Rabbit vaccination with recombinant F. hepatica TGR reduced the worm burden by 96.7% following experimental infection, further supporting the relevance of TGR as a promising target for anti Fasciola treatments.
Asunto(s)
Fasciola hepatica/enzimología , Fascioliasis/inmunología , Glutatión Reductasa/inmunología , Reductasa de Tiorredoxina-Disulfuro/inmunología , Secuencia de Aminoácidos , Animales , Clonación Molecular , ADN Complementario/química , ADN de Helmintos/química , Fasciola hepatica/genética , Fasciola hepatica/inmunología , Fascioliasis/prevención & control , Regulación Enzimológica de la Expresión Génica/inmunología , Glutatión Reductasa/química , Glutatión Reductasa/genética , Interacciones Huésped-Parásitos/inmunología , Datos de Secuencia Molecular , Estructura Secundaria de Proteína , Conejos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Alineación de Secuencia , Análisis de Secuencia de ADN , Reductasa de Tiorredoxina-Disulfuro/química , Reductasa de Tiorredoxina-Disulfuro/genética , Vacunación , Vacunas SintéticasRESUMEN
Adult rat liver contained variant forms of thioredoxin reductase with isoelectric points at pH 4.9 and at approximately pH 4.7 compared to pH 5.1 for the enzyme from Novikoff ascites hepatoma. Fetal and regenerating liver contained only the form with the isoelectric point at pH 4.9. All three enzymes precipitated with and were inhibited by a rabbit antibody to purified enzyme from Novikoff tumor.
Asunto(s)
Carcinoma Hepatocelular/enzimología , Isoenzimas/metabolismo , Neoplasias Hepáticas/enzimología , Hígado/enzimología , NADH NADPH Oxidorreductasas/metabolismo , Reductasa de Tiorredoxina-Disulfuro/metabolismo , Animales , Inmunoelectroforesis , Peso Molecular , Neoplasias Experimentales/enzimología , Ratas , Reductasa de Tiorredoxina-Disulfuro/inmunologíaRESUMEN
Thioredoxin reductase (TR) is a flavoenzyme, containing one selenocysteine (Sec) residue at the penultimate carboxyl-terminus, that catalyzes the NADPH-dependent reduction of oxidized thioredoxin. Sec is encoded by the UGA stop codon in the open reading frame of the mRNA, and the conserved stem-loop structure in 3'-untranslated regions functions as the determinant of Sec incorporation instead of termination of translation. The efficiency of Sec incorporation in Sec-containing enzymes in physiological or selenium (Se)-deficient condition remains unclear. To clarify this, we have developed monoclonal antibodies to human TR, and established a sandwich enzyme-linked immunosorbent assay to determine TR protein content. We observed that the specific activity of cytosolic TR in NCI-H441 cells increased with increasing concentrations of Se in a serum-free medium. The specific activity of TR purified from each cytosol was essentially equal to the calculated specific activity of each cytosolic TR. The Se content of TR increased with increasing concentration of Se in the medium, from 0.32 mol/mol of TR subunit (no SE) to 0.98 mol/mol of TR subunit (500 nM Se), and was directly correlated with the specific activity of TR. When calculated from the cytosolic TR specific activity of human peripheral mononuclear cell, the theoretical efficiency of Sec incorporation in physiological conditions is assumed to be 87%.
Asunto(s)
Selenocisteína/metabolismo , Reductasa de Tiorredoxina-Disulfuro/metabolismo , Animales , Anticuerpos Monoclonales , Línea Celular , Codón/genética , Citosol/metabolismo , Humanos , Técnicas In Vitro , Leucocitos Mononucleares/metabolismo , Oxidación-Reducción , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas , Selenio/metabolismo , Selenocisteína/genética , Reductasa de Tiorredoxina-Disulfuro/genética , Reductasa de Tiorredoxina-Disulfuro/inmunologíaRESUMEN
Specific polyclonal antibodies towards the oxidized form of bovine thioredoxin reductase (TR) have been obtained in rabbits, and purified. The antigenicity was lost upon reduction of TR by NADPH indicating a large conformational change upon reduction of the redox-active disulfide in the enzyme. The antibodies did not cross-react with other bovine NADPH-dependent dehydrogenases. No reactivity was observed with TR from bacteria, yeast or rat and only a slight reaction was obtained with TR from horse. Immunoaffinity purified anti-thioredoxin and anti-glutaredoxin antibodies were used to develop competitive indirect ELISA assays that were validated giving very good linearity, reproducibility, sensitivity and parallelism. The glutaredoxin (Grx) immunoassay is the first quantitative method described to measure the protein. When applied to a battery of calf tissues the contents of Grx varied from 7 to 120 micrograms per gram of fresh tissue. Skeletal and heart muscles gave the lowest values and spleen and salivary glands the highest. However, skeletal muscle showed the highest gluthathione-hydroxyethyl disulfide oxidoreductase specific activity.
Asunto(s)
Inmunohistoquímica , Oxidorreductasas , Proteínas/análisis , Reductasa de Tiorredoxina-Disulfuro/análisis , Tiorredoxinas/análisis , Animales , Anticuerpos/inmunología , Especificidad de Anticuerpos , Bovinos , Ensayo de Inmunoadsorción Enzimática/estadística & datos numéricos , Glutarredoxinas , Músculo Esquelético/química , Miocardio/química , NADP/farmacología , Reproducibilidad de los Resultados , Glándulas Salivales/química , Sensibilidad y Especificidad , Bazo/química , Reductasa de Tiorredoxina-Disulfuro/inmunologíaRESUMEN
Thioredoxin reductase (TrxR), an enzyme belonging to the flavoprotein family of pyridine nucleotide-disulfide oxidoreductases, was isolated from the deoxycholate-soluble extract of the common liver fluke, Fasciola hepatica. Purification to homogeneity of the 60-kDa enzyme from the adult worm was achieved by a combination of ammonium sulfate fractionation, anion exchange, and affinity chromatography on 2',5'-adenosine diphosphate-Sepharose. Using the 5,5'-dithiobis(2-nitrobenzoic acid) assay, the purified TrxR showed a specific activity of 7,117 U min(-1) mg(-1). The enzyme activity was completely inhibited by the presence of the gold compound aurothioglucose (IC50 = 120 nm), indicating that F. hepatica TrxR is a selenoenzyme. Also, the enzyme was capable of reducing disulfide bonds in insulin and was activated by the presence of the reduced form of flavin adenine dinucleotide, properties shared with mammalian TrxRs. Furthermore, the isolated enzyme showed very low glutaredoxin (Grx) activity (0.47 U mg(-1)), but no glutathione reductase activity was detected. Affinity-purified IgGs (20 microg ml(-1)) from the antisera produced against the purified TrxR inhibited its activity about 80% with respect to the control. The enzyme was immunolocalized in cells located within the parenchyma and in the testes, but it was not found in the tegument of the adult fluke.
Asunto(s)
Fasciola hepatica/enzimología , Reductasa de Tiorredoxina-Disulfuro/aislamiento & purificación , Animales , Aurotioglucosa/farmacología , Bovinos , Fraccionamiento Químico , Cromatografía de Afinidad , Cromatografía por Intercambio Iónico , Electroforesis en Gel de Poliacrilamida , Inhibidores Enzimáticos/farmacología , Técnica del Anticuerpo Fluorescente , Immunoblotting , Inmunoglobulina G/inmunología , Reductasa de Tiorredoxina-Disulfuro/antagonistas & inhibidores , Reductasa de Tiorredoxina-Disulfuro/inmunología , Reductasa de Tiorredoxina-Disulfuro/metabolismoRESUMEN
Production of reactive oxygen species (ROS) is the first biological response during a disease outbreak and after injury. ROS are highly reactive molecules that can either endanger cell homeostasis or mediate cell signaling in several physiological pathways, including the immune response. Thioredoxin (Trx) and thioredoxin reductase (TrxR) are the essential components of the thioredoxin system, one of the main intracellular redox systems and are therefore important regulators of ROS accumulation. Through the regulation of the intracellular redox milieu, the thioredoxin system plays a key role within the immune system, linking immunology and free radical science. In this study we have firstly identified TrxRs in fish and used this new sequence information to reevaluate the evolution of the thioredoxin system within the vertebrate lineage. We next measured the expression of rainbow trout (Oncorhynchus mykiss) Trx and TrxR transcripts during infection in vivo and in vitro after stimulation of a macrophage cell line and primary macrophage cultures with pathogen associated molecular patterns (PAMPs). Our results showed that both Trx and TrxR were induced during infection at the transcriptional level, confirming their likely involvement in the innate immune response of fish. Since TrxRs are selenium-containing proteins (selenoproteins), we also measured the modulation of their expression upon organic and inorganic selenium exposure in vitro. TrxR was found to be responsive to selenium exposure in vitro, suggesting that it may represent a key mediator in the selenium modulation of innate immunity. In conclusion, our study highlights the need to investigate the involvement of the cell antioxidant pathways, especially the thioredoxin system, within the immune system of vertebrate species.
Asunto(s)
Oncorhynchus mykiss/inmunología , Isoformas de Proteínas/inmunología , Reductasa de Tiorredoxina-Disulfuro/inmunología , Tiorredoxinas/inmunología , Yersiniosis/inmunología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Línea Celular , Clonación Molecular , Perfilación de la Expresión Génica , Inmunidad Innata , Macrófagos/inmunología , Datos de Secuencia Molecular , Estrés Oxidativo/inmunología , Isoformas de Proteínas/genética , Especies Reactivas de Oxígeno , Selenio/farmacología , Alineación de Secuencia , Análisis de Secuencia de ADN , Reductasa de Tiorredoxina-Disulfuro/biosíntesis , Reductasa de Tiorredoxina-Disulfuro/genética , Tiorredoxinas/biosíntesis , Tiorredoxinas/genética , Transcripción Genética , Yersinia ruckeri/inmunologíaRESUMEN
The thioredoxin system consisting of thioredoxin (Trx), thioredoxin reductase (TrxR), and NADPH is an electron donor for ribonucleotide reductase but has also been implicated in other cellular events, including secretion, growth promotion, regulation of transcription factors, protection against oxidative stress, and apoptosis. Mammalian TrxR is a dimeric flavoprotein with 58 kDa subunits each with a catalytically active selenocysteine residue. To study the function and expression of TrxR, we have produced and characterized, for the first time, monoclonal antibodies against human TrxR. Native placenta TrxR was used for immunization of BALB/c mice, followed by hybridization, cloning, and establishment of hybridomas producing specific antibodies against human TrxR. Three clones of IgG1, kappa subclass, termed anti-TrxR1, anti-TrxR2, and anti-TrxR3, were studied in detail. The isoelectric points (pIs) of the mAbs were 6.5, 6.0, and 6.5, respectively. The affinities (Ka) of the mAbs were 2 x 10(8) M-1. Inhibition ELISA using biotin-labeled versus nonconjugated mAb IgG revealed that all three mAbs recognized one immunodominant epitope. Western blot analysis showed that the antibodies specifically bound to a 58 kDa protein, representing the subunit of TrxR. A Trx-dependent insulin reduction assay was used for analysis of enzymatic activity and the antibodies neutralized the reductase activity.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Anticuerpos Monoclonales/aislamiento & purificación , Reductasa de Tiorredoxina-Disulfuro/inmunología , Animales , Afinidad de Anticuerpos , Especificidad de Anticuerpos , Femenino , Humanos , Ratones , Ratones Endogámicos BALB CRESUMEN
Egg granuloma formation during schistosome infections is mediated by CD4(+) T helper (Th) cells sensitized to egg antigens; however, most of the relevant sensitizing egg antigens are still unknown. Here we show that schistosome thioredoxin peroxidase (TPx)-1 is a novel T- and B-cell egg antigen in schistosome-infected mice. CD4(+) Th cell responses to fractionated egg components identified a significant response against a 26-kDa antigen; a partial amino acid sequence of this antigen was found to be identical to that of Schistosoma mansoni TPx-1. The native TPx-1 elicited significant proliferative responses as well as gamma interferon (IFN-gamma), interleukin-2 (IL-2), IL-4, and IL-5 secretion in CD4(+) cells from 8.5-week-infected CBA and C57BL/6 mice. By comparison, recombinant TPx-1 elicited a smaller, more type 1-polarized response, with significant production of IFN-gamma and IL-2, less IL-5, and essentially no IL-4. In C57BL/6 mice the responses to TPx-1 were relatively more prominent than that directed against the major egg antigen, Sm-p40, whereas in CBA mice the reverse was true. B-cell responses were also monitored in infected C57BL/6, C3H, CBA, and BALB/c mice. All strains had significant antibody levels against the TPx-1 protein, but the most significant antibody production ensued following parasite oviposition. TPx-1 was localized in eggs and shown to be secreted by eggs. The identification of egg antigens is important to understand the specific basis of granuloma formation in schistosome infections and may prove to be useful in strategies to ameliorate pathological responses.
Asunto(s)
Antígenos Helmínticos/inmunología , Linfocitos B/inmunología , Linfocitos T CD4-Positivos/inmunología , Proteínas del Huevo/inmunología , Activación de Linfocitos , Esquistosomiasis mansoni/inmunología , Reductasa de Tiorredoxina-Disulfuro/inmunología , Secuencia de Aminoácidos , Animales , Femenino , Inmunohistoquímica , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , Peso MolecularRESUMEN
Thioredoxin reductase (E.C.1.6.4.5.) has been purified to about 95% homogeneity from the Novikoff ascites rat tumor. The enzyme contained two subunits of approximately 58,000 daltons, with one FAD per subunit. The amino acid analysis is reported. An immunoadsorbent was prepared and used for affinity chromatography in order to improve the yield of the enzyme.
Asunto(s)
Carcinoma Hepatocelular/enzimología , Neoplasias Hepáticas/enzimología , NADH NADPH Oxidorreductasas/aislamiento & purificación , Reductasa de Tiorredoxina-Disulfuro/aislamiento & purificación , Aminoácidos/análisis , Animales , Femenino , Técnicas de Inmunoadsorción , Peso Molecular , Neoplasias Experimentales/enzimología , Ratas , Reductasa de Tiorredoxina-Disulfuro/análisis , Reductasa de Tiorredoxina-Disulfuro/inmunologíaRESUMEN
In a patient with ovarian cancer, anti-nuclear antibodies were found by indirect immunofluorescence analysis in cultured cells. The patient's serum was used for immunoscreening of a cDNA library. Three overlapping positive clones were partial cDNA clones of thioredoxin reductase (TR), which is a member of the pyridine nucleotide-disulfide oxidoreductase family of flavoenzymes. Human autoantibodies affinity-purified with bacterial recombinant TR reacted to approximately 60-kDa polypeptide in HeLa extract in immunoblotting analysis and strongly stained around chromosome of metaphase of HeLa cells in immunofluorescence studies. Autoantibodies against recombinant TR were not found in our 100 patients with systemic autoimmune diseases. Immunoblotting analysis with bovine purified TR confirmed that the human serum had reactivities to TR. This is the first report of autoantibodies against a redox member in a cancer patient. TR has diverse functions in cell growth, death, and transformation through redox modulation with thioredoxin. Since dysregulation of TR in cancer cells is considered to be involved in some redox states of tumorigenesis, the observed antibody response in this patient could have arisen due to immunoreaction to the abnormally regulated protein.
Asunto(s)
Autoanticuerpos/inmunología , Neoplasias Ováricas/inmunología , Reductasa de Tiorredoxina-Disulfuro/inmunología , Autoanticuerpos/sangre , Autoanticuerpos/aislamiento & purificación , Núcleo Celular/inmunología , Clonación Molecular , Femenino , Técnica del Anticuerpo Fluorescente , Células HeLa , Humanos , Immunoblotting , Japón , Poli Adenosina Difosfato Ribosa/inmunología , Proteínas Recombinantes/inmunología , Reductasa de Tiorredoxina-Disulfuro/genéticaRESUMEN
Thioredoxins h are ubiquitous proteins reduced by NADPH- thioredoxin reductase (NTR). They are able to reduce disulphides in target proteins. In monocots, thioredoxins h accumulate at high level in seeds and show a predominant localization in the nucleus of seed cells. These results suggest that the NTR-thioredoxin h system probably plays an important role in seed physiology. To date, the study of this system in monocots is limited by the lack of information about NTR. In the present study, we describe the cloning of a full-length cDNA encoding NTR from wheat ( Triticum aestivum ). The polypeptide deduced from this cDNA shows close similarity to NTRs from Arabidopsis, contains FAD- and NADPH-binding domains and a disulphide probably interacting with the disulphide at the active site of thioredoxin h. Wheat NTR was expressed in Escherichia coli as a His-tagged protein. The absorption spectrum of the purified recombinant protein is typical of flavoenzymes. Furthermore, it showed NADPH-dependent thioredoxin h reduction activity, thus confirming that the cDNA clone reported in the present study encodes wheat NTR. Using the His-tagged NTR and TRXhA (wheat thioredoxin h ), we successfully reconstituted the wheat NTR-thioredoxin h system in vitro, as shown by the insulin reduction assay. A polyclonal antibody was raised against wheat NTR after immunization of rabbits with the purified His-tagged protein. This antibody efficiently detected a single polypeptide of the corresponding molecular mass in seed extracts and it allowed the analysis of the pattern of accumulation of NTR in different wheat organs and developmental stages. NTR shows a wide distribution in wheat, but, surprisingly, its accumulation in seeds is low, in contrast with the level of thioredoxins h.