RESUMEN
Pancreatic ß cells are responsible for insulin secretion and are important for glucose regulation in a healthy body and diabetic disease patient without prelabeling of islets. While the conventional biomarkers for diabetes have been glucose and insulin concentrations in the blood, the direct determination of the pancreatic ß cell mass would provide critical information for the disease status and progression. By combining fluorination and diversity-oriented fluorescence library strategy, we have developed a multimodal pancreatic ß cell probe PiF for both fluorescence and for PET (positron emission tomography). By simple tail vein injection, PiF stains pancreatic ß cells specifically and allows intraoperative fluorescent imaging of pancreatic islets. PiF-injected pancreatic tissue even facilitated an antibody-free islet analysis within 2 h, dramatically accelerating the day-long histological procedure without any fixing and dehydration step. Not only islets in the pancreas but also the low background of PiF in the liver allowed us to monitor the intraportal transplanted islets, which is the first in vivo visualization of transplanted human islets without a prelabeling of the islets. Finally, we could replace the built-in fluorine atom in PiF with radioactive 18F and successfully demonstrate in situ PET imaging for pancreatic islets.
Asunto(s)
Colorantes Fluorescentes/química , Células Secretoras de Insulina/citología , Xantenos/química , Animales , Diabetes Mellitus Experimental/patología , Fluorescencia , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/farmacocinética , Colorantes Fluorescentes/toxicidad , Humanos , Células Secretoras de Insulina/trasplante , Trasplante de Islotes Pancreáticos , Hígado/citología , Ratones Endogámicos C57BL , Ratones Endogámicos ICR , Tomografía de Emisión de Positrones , Ratas , Xantenos/síntesis química , Xantenos/farmacocinética , Xantenos/toxicidadRESUMEN
Melanoma is characterized by high malignancy and early onset of metastasis. Epithelial-to-mesenchymal transition (EMT) is an early event during tumor metastasis. Tumor cells that develop EMT can escape apoptosis, but they are vulnerable to ferroptosis inducers. Gambogenic acid (GNA), a xanthone found in Gamboge, has cytotoxic effects in highly invasive melanoma cells. This study investigated the anti-melanoma effect and mechanism of action of GNA in TGF-ß1-induced EMT melanoma cells. We found that GNA significantly inhibited the invasion, migration and EMT in melanoma cells, and these cells exhibited small mitochondrial wrinkling (an important feature of ferroptosis). An iron chelator, but not an apoptosis inhibitor or a necrosis inhibitor, abolished the inhibitory effects of GNA on proliferation, invasion and migration of TGF-ß1-stimulated melanoma cells. GNA upregulated the expression of p53, solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) in the model cells, contributing to the mechanisms underlying GNA-induced ferroptosis. Collectively, our findings suggest that GNA induces ferroptosis in TGF-ß1-stimulated melanoma cells via the p53/SLC7A11/GPX4 signaling pathway.
Asunto(s)
Medicamentos Herbarios Chinos/toxicidad , Transición Epitelial-Mesenquimal/efectos de los fármacos , Ferroptosis/efectos de los fármacos , Melanoma/metabolismo , Neoplasias Cutáneas/metabolismo , Xantenos/toxicidad , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/fisiología , Medicamentos Herbarios Chinos/uso terapéutico , Transición Epitelial-Mesenquimal/fisiología , Ferroptosis/fisiología , Humanos , Melanoma/tratamiento farmacológico , Melanoma/patología , Neoplasias Cutáneas/tratamiento farmacológico , Neoplasias Cutáneas/patología , Xantenos/uso terapéuticoRESUMEN
H2S is an important endogenous gasotransmitter, and its detection in living systems is of great significance. Especially, selective and sensitive near-infrared (NIR) fluorescent H2S probes with rapid response and large Stokes shift are highly desirable because of their superiority for in vivo detection. Probes with nitrobenzoxadiazole (NBD) ether as reaction sites have been well-explored recently to detect biothiols or H2S/biothiols simultaneously, rather than to detect H2S selectively. In this work, a new NBD ether-based NIR fluorescent probe was developed, which was unexpectedly found to show high selectivity for H2S over various other analytes including biothiols, making it practical for specific detection of H2S both in vitro and in vivo. Upon response to H2S, this probe showed rapid and significant turn-on NIR emission changes centered at 744 nm within 3 min, together with a remarkable large Stokes shift (166 nm) and high sensitivity (LOD: 26 nM). Moreover, imaging exogenous and endogenous H2S in living cells and rapid imaging of H2S in living mice with this probe was successfully applied with excellent performance.
Asunto(s)
4-Cloro-7-nitrobenzofurazano/análogos & derivados , Colorantes Fluorescentes/química , Sulfuro de Hidrógeno/análisis , Xantenos/química , 4-Cloro-7-nitrobenzofurazano/síntesis química , 4-Cloro-7-nitrobenzofurazano/toxicidad , Animales , Línea Celular Tumoral , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/toxicidad , Humanos , Límite de Detección , Ratones , Microscopía Confocal/métodos , Microscopía Fluorescente/métodos , Imagen Óptica , Xantenos/síntesis química , Xantenos/toxicidadRESUMEN
Four new xanthene derivatives, penicixanthenes A-D (1-4), and one known compound 5 were isolated from a marine mangrove endophytic fungus Penicillium sp. JY246 that was obtained from the stem of Ceriops tagal. Their structures were determined by detailed NMR, MS spectroscopic data, modified Mosher's method, and calculated electronic circular dichroism data. All of the isolated compounds were examined for insecticidal activity. Compounds 2 and 3 showed growth inhibition activity against newly hatched larvae of Helicoverpa armigera Hubner with the IC50 values 100 and 200 µg/mL, respectively, and compounds 1, 3, and 4 showed insecticidal activity against newly hatched larvae of Culex quinquefasciatus with LC50 values of 38.5 (±1.16), 11.6 (±0.58), and 20.5 (±1) µg/mL, respectively. The four xanthene derivatives have the potential to be developed as new biopesticides.
Asunto(s)
Agentes de Control Biológico/toxicidad , Endófitos/metabolismo , Penicillium/metabolismo , Xantenos/toxicidad , Animales , Agentes de Control Biológico/aislamiento & purificación , Agentes de Control Biológico/metabolismo , Culex/efectos de los fármacos , Concentración 50 Inhibidora , Larva , Mariposas Nocturnas/efectos de los fármacos , Rhizophoraceae/microbiología , Humedales , Xantenos/aislamiento & purificación , Xantenos/metabolismoRESUMEN
Peroxynitrite (ONOO-) plays important roles in the regulation of many physiological and pathological processes, and an increase in its levels is related to numerous diseases. Thus, accurate detection of ONOO- in physiological conditions is imperative for elucidating its functions. However, studies on high signal-to-noise-ratio (SNR) fluorescence imaging of ONOO-in vivo for its detection are currently lacking. Thus, a novel NIR xanthene fluorescence probe (NOF2) for the endogenous detection of ONOO- is designed and synthesized. The fluorescence of the NOF2 probe is pre-quenched by the hydroxyl protection group of diphenyl phosphinate. Additionally, the NOF2 probe exhibits good selectivity and sensitivity for ONOO- with a low detection limit of 0.40 µM. Importantly, the NOF2 probe displays good performances for the detection of endogenous ONOO- not only in living cells but also in a mouse inflammation model. This demonstrates its great potential for applications involving the detection of ONOO- both in vitro and in vivo to explore the roles of ONOO- in different physiological systems.
Asunto(s)
Colorantes Fluorescentes/química , Inflamación/metabolismo , Ácido Peroxinitroso/análisis , Ácidos Fosfínicos/química , Xantenos/química , Animales , Fluorescencia , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/toxicidad , Humanos , Límite de Detección , Células MCF-7 , Ratones , Ácidos Fosfínicos/síntesis química , Ácidos Fosfínicos/toxicidad , Células RAW 264.7 , Espectrometría de Fluorescencia/métodos , Xantenos/síntesis química , Xantenos/toxicidadRESUMEN
A diverse library of chromene-xanthene hybrids were synthesized through intramolecular Friedel-Crafts reaction of the arenoxy carbinols. Examples include first incorporation of amino acid tyrosine into xanthene skeletons with polar functionalities. A careful structural evaluation revealed that tyrosine crafted chromene-xanthene hybrids exhibited good activities against breast cancer cell lines MCF-7, MDA-MB-231. The lead compound 16 displays significant cell cycle arrest at G1 phase and induces apoptosis in MDA-MB-231 cells.
Asunto(s)
Antineoplásicos/farmacología , Benzopiranos/farmacología , Neoplasias de la Mama/tratamiento farmacológico , Xantenos/farmacología , Antineoplásicos/síntesis química , Antineoplásicos/toxicidad , Apoptosis/efectos de los fármacos , Benzopiranos/síntesis química , Benzopiranos/toxicidad , Línea Celular Tumoral , Puntos de Control de la Fase G1 del Ciclo Celular/efectos de los fármacos , Células HEK293 , Humanos , Estructura Molecular , Tamoxifeno/farmacología , Xantenos/síntesis química , Xantenos/toxicidadRESUMEN
Previously, evaluation of sodium metavanadate (NaVO3) cytotoxicity after 24 h exposure of Chinese hamster ovary K1 (CHO-K1) cells revealed different sensitivity of the in vitro assays used starting from the neutral red (NR, 3-amino-7-dimethylamino-2-methylphenazine hydrochloride) test (detecting lysosomal and possibly the Golgi apparatus damage) as the most sensitive followed by the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) and resazurin (7-hydroxy-3H-phenoxazin-3-one-10-oxide) tests (mitochondrial disruption). The trypan blue (TB) staining (plasma membrane permeability) showed cytotoxicity of NaVO3 at a much higher NaVO3 concentration than the above-mentioned assays. In the current study, using the same experimental approach, we have assessed the toxicity of vanadyl sulphate (VOSO4) and compared the obtained results with NaVO3 action. Unlike metavanadate, VOSO4 treatment at 24 h resulted in similar sensitivity of the NR and resazurin tests. Nevertheless, following the 48-h incubation with VOSO4, the NR test showed markedly higher sensitivity than the resazurin test when comparing the half maximal inhibitory concentration values (61 and 110 µM for the NR and resazurin test, respectively, p < 0.05). The TB staining method was the least susceptible for detecting vanadyl cytotoxicity at each exposure time point. In summary, both the NR and resazurin tests can be advocated as similarly sensitive in detection of VOSO4-induced cytotoxicity in the CHO-K1 cell line at 24 h. However, the longer incubation time with VOSO4 showed that the NR test is more sensitive than the resazurin assay. The differences in the results between the cytotoxicity tests employed probably arise from dissimilar susceptibility of the endpoints (targets) measured with these tests to the damage by vanadium. Considering this, the current and the previous studies highlight the role of lysosomes (and possibly the Golgi apparatus) apart from mitochondria in the toxicity mechanism induced by inorganic vanadium in mammalian cells.
Asunto(s)
Supervivencia Celular/efectos de los fármacos , Pruebas de Toxicidad/métodos , Compuestos de Vanadio/toxicidad , Animales , Bioensayo , Células CHO , Cricetinae , Cricetulus , Aparato de Golgi/efectos de los fármacos , Concentración 50 Inhibidora , Lisosomas/efectos de los fármacos , Mitocondrias/efectos de los fármacos , Rojo Neutro/química , Oxazinas/toxicidad , Sensibilidad y Especificidad , Sales de Tetrazolio/toxicidad , Vanadatos/toxicidad , Xantenos/toxicidadRESUMEN
We baked low-calorie bread by mixing charred cellulose granules with wheat flour, using the charred cellulose granules to eliminate toxic xanthene food dyes contained in processed foods from the alimentary canal. The size of the charred cellulose granules played an important role in determining good breadmaking properties in respect of the bread height (mm) and specific volume (SV, cm3/g). Charred cellulose granules with a diameter above 270 µm were blended with wheat flour at 10% to obtain bread with a lower caloric content (1020 kcal/gram of bread) than the control bread (1126 kcal) made solely from wheat flour. The charred cellulose granules taken out from the bread adsorbed toxic xanthene food dyes at around pH 6.5, such that toxic food dyes taken into the alimentary canal were excreted in the feces with the non-digestible cellulose granules.
Asunto(s)
Pan/análisis , Celulosa/química , Culinaria/métodos , Colorantes de Alimentos/metabolismo , Tracto Gastrointestinal/metabolismo , Triticum , Xantenos/toxicidad , Adsorción , Heces/química , Harina , Colorantes de Alimentos/química , Colorantes de Alimentos/aislamiento & purificación , Colorantes de Alimentos/toxicidad , Dureza , Calor , Humanos , Concentración de Iones de Hidrógeno , Odorantes/análisis , Xantenos/química , Xantenos/aislamiento & purificación , Xantenos/metabolismoRESUMEN
A novel water-soluble near-infrared fluorescent probe named QX-P with simple synthesis is developed. QX-P has high sensitivity and selectivity to ALP. Moreover, the probe can not only visualize ALP activity in four cell lines, but also real-time image ALP activity during the diagnosis and treatment of diabetes in mice.
Asunto(s)
Fosfatasa Alcalina/metabolismo , Diabetes Mellitus Experimental/enzimología , Colorantes Fluorescentes/química , Animales , Línea Celular Tumoral , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/toxicidad , Fluorometría , Humanos , Límite de Detección , Ratones , Compuestos de Quinolinio/síntesis química , Compuestos de Quinolinio/química , Compuestos de Quinolinio/toxicidad , Espectrometría de Fluorescencia , Xantenos/síntesis química , Xantenos/química , Xantenos/toxicidadRESUMEN
Photosensitizers produce cytotoxic reactive oxygen species (ROS) upon light illumination, but it is difficult to ablate cells of a specific type (e.g., tumor cells) in the presence of other cell populations, because of the limited precision with which light illumination can be directed to small areas. Here, we report a strategy to achieve cell type-specific ablation by using an enzyme-activated off/on switch for oxidative stress induction. In the unactivated photosensitizer, induction of oxidative stress is quenched by intramolecular electron transfer. However, the target cells express an enzyme that hydrolyzes a substrate moiety of the photosensitizer and the activated photosensitizer induces oxidative stress. As proof of concept, we designed and synthesized a xanthene-based photosensitizer, TGI-betaGal, whose oxidative stress induction ability is switched on following hydrolysis reaction with beta-galactosidase, a widely used gene marker. TGI-betaGal could selectively ablate lacZ-positive cells, whereas it showed no toxicity to lacZ-negative cells, upon light illumination.
Asunto(s)
Fármacos Fotosensibilizantes/química , beta-Galactosidasa/metabolismo , Línea Celular , Transporte de Electrón , Activación Enzimática , Colorantes Fluorescentes/química , Humanos , Luz , Estrés Oxidativo , Fármacos Fotosensibilizantes/toxicidad , Xantenos/química , Xantenos/toxicidadRESUMEN
Mu-opioid analgesics are a mainstay in the treatment of acute and chronic pain of multiple origins, but their side effects, such as constipation, respiratory depression, and abuse liability, adversely affect patients. The recent demonstration of the up-regulation and membrane targeting of the delta-opioid receptor (DOR) following inflammation and the consequent enhanced therapeutic effect of delta-opioid agonists have enlivened the search for delta-opioid analgesic agents. JNJ-20788560 [9-(8-azabicyclo-[3.2.1]oct-3-ylidene)-9H-xanthene-3-carboxylic acid diethylamide] had an affinity of 2.0 nM for DOR (rat brain cortex binding assay) and a naltrindole sensitive DOR potency of 5.6 nM (5'-O-(3-[(35)S]thio)triphosphate assay). The compound had a potency of 7.6 mg/kg p.o. in a rat zymosan radiant heat test and of 13.5 mg/kg p.o. in a rat Complete Freund's adjuvant RH test but was virtually inactive in an uninflamed radiant heat test. In limited studies, tolerance was not observed to the antihyperalgesic or antinociceptive effects of the compound. Unlike ibuprofen, JNJ-20788560 did not produce gastrointestinal (GI) erosion. Although morphine reduced GI motility at all doses tested and reached nearly full effect at the highest dose, JNJ-20788560 did not retard transit at the lowest dose and reached only 11% reduction at the highest dose administered. Unlike morphine, JNJ-20788560 did not exhibit respiratory depression (blood gas analysis), and no withdrawal signs were precipitated by the administration of opioid (mu or delta) antagonists. Coupled with the previously published lack of self-administration behavior of the compound by alfentanil-trained primates, these findings strongly recommend delta-opioid agonists such as JNJ-20788560 for the relief of inflammatory hyperalgesia.
Asunto(s)
Analgésicos Opioides , Compuestos de Azabiciclo/farmacología , Hiperalgesia/tratamiento farmacológico , Receptores Opioides delta/agonistas , Insuficiencia Respiratoria/inducido químicamente , Trastornos Relacionados con Sustancias/fisiopatología , Xantenos/farmacología , Alfentanilo/farmacología , Animales , Compuestos de Azabiciclo/efectos adversos , Compuestos de Azabiciclo/toxicidad , Cricetinae , Tolerancia a Medicamentos , Motilidad Gastrointestinal/efectos de los fármacos , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Calor , Irritantes/toxicidad , Masculino , Ratones , Dimensión del Dolor/efectos de los fármacos , Ratas , Ratas Wistar , Receptores Opioides delta/metabolismo , Insuficiencia Respiratoria/fisiopatología , Convulsiones/inducido químicamente , Autoadministración , Úlcera Gástrica/inducido químicamente , Úlcera Gástrica/patología , Síndrome de Abstinencia a Sustancias/psicología , Xantenos/efectos adversos , Xantenos/toxicidad , ZimosanRESUMEN
A simple and expedient method for the synthesis of a series of 14-aryl-14H-dibenzo[a.j]xanthenes is described through a one-pot condensation of beta-naphthol with aryl aldehydes catalysed by TaCl5 under solvent-free conventional heating. The major advantages of the present method are: high yields, less reaction time, solvent-free condition and easy purification of the products. The synthesized 14-aryl-14H-dibenzo[a.j]xanthenes were evaluated against a panel of six human cancer lines of different tissues. Synthesized compound 3o showed IC50 of 37.9 and 41.3 microM against Colo-205 and 502713, respectively, whereas 3g showed IC50 of 41.9 microM against Colo-205.
Asunto(s)
Citotoxinas/síntesis química , Xantenos/síntesis química , Aldehídos/química , Catálisis , Línea Celular Tumoral , Cloruros/química , Citotoxinas/química , Citotoxinas/toxicidad , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Naftoles/química , Tantalio/química , Xantenos/química , Xantenos/toxicidadRESUMEN
Studies of amyloid proteins have gradually become a hot topic. Nevertheless, very few effective drugs and treatments is available to cope with amyloid diseases. New molecules that can inhibit the protein fibrillation are highly anticipated. Insulin is one of the popular amyloid protein research models. On the other hand, resazurin and resorufin are widely known as a redox pair. We describes here an unexpected finding that resazurin plays a role in modulating insulin fibrillation, whereas resorufin doesn't. We hypothesize that the positively charged insulin at low pH can combine with the negatively charged resazurin due to electrostatic interaction, through which resazurin inhibited the process of insulin fibrillation. This effect was characterized and verified by using various biochemical, spectroscopic and imaging tools. This inhibition of this biocompatible dye can be achieved at various stages of fibrillation, suggesting the toxicity of the protein fibrils can be eliminated by using resazurin.
Asunto(s)
Insulina/química , Insulina/metabolismo , Agregado de Proteínas , Escherichia coli/efectos de los fármacos , Modelos Moleculares , Oxazinas/química , Oxazinas/metabolismo , Oxazinas/toxicidad , Oxidación-Reducción , Conformación Proteica , Xantenos/química , Xantenos/metabolismo , Xantenos/toxicidadRESUMEN
Ergot, fungal genus Claviceps, are worldwide distributed grass pathogens known for their production of toxic ergot alkaloids (EAs) and the great agricultural impact they have on both cereal crop and farm animal production. EAs are traditionally considered as the only factor responsible for ergot toxicity. Using broad sampling covering 13 ergot species infecting wild or agricultural grasses (including cereals) across Europe, USA, New Zealand, and South Africa we showed that the content of ergochrome pigments were comparable to the content of EAs in sclerotia. While secalonic acids A-C (SAs), the main ergot ergochromes (ECs), are well known toxins, our study is the first to address the question about their contribution to overall ergot toxicity. Based on our and published data, the importance of SAs in acute intoxication seems to be negligible, but the effect of chronic exposure needs to be evaluated. Nevertheless, they have biological activities at doses corresponding to quantities found in natural conditions. Our study highlights the need for a re-evaluation of ergot toxicity mechanisms and further studies of SAs' impact on livestock production and food safety.
Asunto(s)
Claviceps/química , Alcaloides de Claviceps/toxicidad , Micotoxinas/toxicidad , Xantenos/toxicidad , Apoptosis/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Alcaloides de Claviceps/análisis , Células HeLa , Humanos , Células Jurkat , Mitocondrias/efectos de los fármacos , Micotoxinas/análisis , Micotoxinas/farmacología , Xantenos/análisisRESUMEN
We developed a photoacoustic and fluorescent dual-mode imaging probe, CCYS, for the detection of cysteine with high selectivity and sensitivity in a living system for the first time. By using CCYS, we found that the limited synthesis of glutathione in pulmonary fibrosis was not caused by cysteine deficiency.
Asunto(s)
Acrilatos/química , Cisteína/análisis , Colorantes Fluorescentes/química , Glutatión/biosíntesis , Fibrosis Pulmonar/fisiopatología , Acrilatos/síntesis química , Acrilatos/toxicidad , Animales , Cisteína/química , Femenino , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/toxicidad , Indoles/síntesis química , Indoles/química , Indoles/toxicidad , Límite de Detección , Lisosomas/metabolismo , Ratones , Microscopía Confocal/métodos , Microscopía Fluorescente/métodos , Técnicas Fotoacústicas/métodos , Fibrosis Pulmonar/diagnóstico , Xantenos/síntesis química , Xantenos/química , Xantenos/toxicidadRESUMEN
Three compounds that share specific antimitochondrial properties are gossypol, rhodamine-123, and lonidamine. We compare the antiproliferative activities of these drugs against six human cell lines derived from breast (T47-D), pancreas (MiaPaCa, RWP-2), prostate (DU-145), colon (HCT-8), and cervix (HeLa) carcinomas. Tumor cells enriched in cathodal LDH isozymes (LDH4 and LDH5) are significantly more sensitive to gossypol and rhodamine-123. When compared for ability to inhibit growth of human marrow in soft agar, 10 microM gossypol shows little effect on colony formation whereas 10 microM rhodamine-123 completely prevents stem cell growth, suggesting that gossypol may have the most favorable therapeutic index. Within 24 h of drug administration, there is a relative increase in intracellular inorganic phosphate pools and a marked decline in soluble high-energy phosphates in sensitive tumor cells, as measured by 31P magnetic resonance spectroscopy. These studies suggest that specific antimitochondrial agents might be selectively administered on the basis of tumor LDH isozyme content and noninvasively monitored for antiproliferative activity by 31P spectroscopy.
Asunto(s)
Gosipol/toxicidad , L-Lactato Deshidrogenasa/metabolismo , Rodaminas/toxicidad , Xantenos/toxicidad , División Celular/efectos de los fármacos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Ensayo de Unidades Formadoras de Colonias , Femenino , Células Madre Hematopoyéticas/citología , Células Madre Hematopoyéticas/efectos de los fármacos , Humanos , Isoenzimas , Espectroscopía de Resonancia Magnética/métodos , Masculino , Neoplasias/patología , Fósforo , Rodamina 123 , Ensayo de Tumor de Célula MadreRESUMEN
Versicolorin A (VA) and sterigmatocystin (ST) are biosynthetic precursors of aflatoxin B1 (AFB1). The carcinogenicity of these compounds relative to AFB1 was determined with the use of rainbow trout (Salmo gairdneri) embryo exposure. Exposure of 14-day rainbow trout embryos to a 0.5-ppm aqueous suspension of ST for 1 hour produced a 13% incidence of hepatocellular carcinomas among survivors 1 year later. Similar exposure of trout eggs to a 0.5-ppm solution of AFB1 produced a 53% incidence among survivors. Subsequent exposure of 21-day rainbow trout embryos to 5- and 25-ppm solutions of VA resulted in hepatocellular carcinoma incidences among survivors of 42 and 68%, respectively, at 12 months. A 0.5-ppm AFB1 positive control group had a 68% incidence among survivors of hepatocellular carcinomas at 1 year. These results established the carcinogenicity of VA for the first time and confirmed previous reports of ST carcinogenicity. Both compounds were of sufficient potencies to warrant caution as possible human health hazards.
Asunto(s)
Aflatoxinas/toxicidad , Antraquinonas/toxicidad , Neoplasias Hepáticas Experimentales/inducido químicamente , Salmonidae/fisiología , Esterigmatocistina/toxicidad , Trucha/fisiología , Xantenos/toxicidad , Animales , Neoplasias Hepáticas Experimentales/patología , Trucha/embriologíaRESUMEN
Because both Rhodamine 123 (R123) and hyperthermia have been shown to be cytotoxic, we examined their effect, independently and in combination, on five different human malignant cell lines in vitro and on cultured melanoma cells grown intradermally in nude mice. The cell lines examined include two human melanomas, UCLA-SO-M14 and UCLA-SO-M21, the colon cancer cell line HT29, the human lung cancer cell line P3, and the human breast cancer cell line B231. R123 and hyperthermia, when used in combination, were found to be cytotoxic for these five different human malignant cell lines in vitro. The two agents together appear to enhance the cytotoxic effect of each alone, as documented by synergistic ratios ranging from 2.31 to 45 for the different cell lines. In the "nude" mouse model, animals were treated with a combination of R123 and hyperthermia (43 degrees C for 90 min). A statistically significant (P = 0.04) decrease in tumor growth rate was observed when compared with the rate of tumor growth in untreated animals. The results suggest a potential role for R123 in combination with hyperthermia in the treatment of malignant cells.
Asunto(s)
Calor , Rodaminas/toxicidad , Xantenos/toxicidad , Animales , Humanos , Técnicas In Vitro , Ratones , Ratones Desnudos , Trasplante de Neoplasias , Neoplasias Experimentales/terapia , Rodamina 123 , Rodaminas/uso terapéutico , Células Tumorales CultivadasRESUMEN
Cross-resistance to rhodamine 123 (Rho-123) has been found in Adriamycin (ADM)-resistant and daunorubicin (DNR)-resistant Friend leukemia cell variants. Cytotoxicity in sensitive cells correlates with the intracellular amount of Rho-123, as determined by high-pressure liquid chromatography. Differential resistance coincides with Rho-123 accumulation which in sensitive cells was 20-fold higher than in resistant cells after 180 min of treatment. Sodium azide, which has been shown to inhibit ADM efflux and consequently increase drug accumulation in ADM-resistant cells, did not inhibit Rho-123 efflux. The difference in Rho-123 accumulation between sensitive and resistant cells correlates with cytotoxicity, which is in contrast to what has been found in these cells when treated with either ADM or DNR. Moreover, in contrast to the known effects of ADM and DNR on macromolecular synthesis, Rho-123 in sensitive cells was found to inhibit protein synthesis but had no effect on DNA or RNA synthesis. At Rho-123 doses which inhibited protein synthesis, drug localization changed from mitochondrial specific to generalized cytoplasmic. This effect was never achieved in resistant cells, even with prolonged drug exposure. The relevance of these findings is that different mechanisms of resistance to different drug types can be identified in the same cells even though similar resistance occurs. The similarity in resistance need not share a common mechanism. Although the drugs are effluxed more efficiently in resistant cells, the mechanisms for resistance in each case seem to differ. In the case of ADM and DNR, it appears to be multifactorial, whereas with Rho-123, total intracellular accumulation seems to be most important.
Asunto(s)
Daunorrubicina/toxicidad , Doxorrubicina/toxicidad , Leucemia Experimental/patología , Rodaminas/toxicidad , Xantenos/toxicidad , Animales , Transporte Biológico , Línea Celular , Resistencia a Medicamentos , Cinética , Ratones , Rodamina 123 , Rodaminas/metabolismoRESUMEN
Sterigmatocystin, a mycotoxin produced by Aspergillus versicolor, Aspergillus sydowi, Aspergillus nidulans, and a species of Bipolaris, was given to newborn BALB/c X DBA/2F1 (hereafter referred to as CD2F1) mice by a single s.c. administration in 1% gelatin suspension. In an acute toxicity study, the maximum tolerated dose of sterigmatocystin was 5 mug/g body weight. In a chronic study, a single s.c. injection of 5, 1, or 0.5 mug/g body weight gave rise to high incidences of lung and liver adenomas when the animals were killed at the end of 1 year. The incidence of both tumors in mice at the dose of 5 mug/g body weight was statistically significant, and the incidences of lung tumor in female mice and of liver tumor in male mice at the dose of 1 mug/g body weight were also statistically significant, compared with tumors in control mice. Other tumors also were induced in treated mice (two malignant lymphomas and one adenoma of the submaxillary gland), in contrast to a zero incidence in vehicle control mice. These results confirm that a small quantity of sterigmatocystin induces tumors of lung and liver and that the dose of sterigmatocystin is related to the incidence of tumors in mice.