RESUMO
The microbiological diagnosis of mycoplasma and ureaplasma infections has always been limited due to the fastidious growth of these microorganisms, as well as the lack of commercially prepared growth media, absence of rapid diagnostic procedures, and the clinical perception that these organisms are less significant in the infectious diseases setting. During the last few years, this situation has substantially improved due to the commercial availability of culture media, the development of rapid serological techniques, and, in particular, to the introduction of nucleic acid amplification assays, commercially available or "in-house" preparations. Despite the lack of proper standardisation and validation of the molecular and serological techniques, methodological advances have led to an increased detection of these microorganisms and, consequently, a greater appreciation of their clinical relevance.
Assuntos
Técnicas Bacteriológicas , Infecções por Mycoplasma/diagnóstico , Infecções por Ureaplasma/diagnóstico , Anticorpos Antibacterianos/sangue , Meios de Cultura , DNA Bacteriano/sangue , Feminino , Doenças Urogenitais Femininas/diagnóstico , Doenças Urogenitais Femininas/microbiologia , Humanos , Masculino , Doenças Urogenitais Masculinas/diagnóstico , Doenças Urogenitais Masculinas/microbiologia , Espectrometria de Massas , Mycoplasma/efeitos dos fármacos , Mycoplasma/crescimento & desenvolvimento , Mycoplasma/isolamento & purificação , Mycoplasma/patogenicidade , Infecções por Mycoplasma/microbiologia , Pneumonia por Mycoplasma/diagnóstico , Pneumonia por Mycoplasma/microbiologia , Gravidez , Complicações Infecciosas na Gravidez/diagnóstico , Complicações Infecciosas na Gravidez/microbiologia , Reação em Cadeia da Polimerase em Tempo Real , Testes Sorológicos/métodos , Especificidade da Espécie , Manejo de Espécimes , Análise Espectral Raman , Ureaplasma/efeitos dos fármacos , Ureaplasma/crescimento & desenvolvimento , Ureaplasma/isolamento & purificação , Ureaplasma/patogenicidade , Infecções por Ureaplasma/microbiologia , VirulênciaRESUMO
Streptococcus agalactiae (GBS) remains the leading cause of meningitis and neonatal sepsis in the world, and causes disease in pregnant and puerperal women. This is a retrospective study of GBS infections on women of childbearing age living in Comunitat Valenciana, Spain (years 2009-2014) and GBS colonization rate on pregnant women attending Hospital La Fe (years 2013-2015) according to their origin. An aggregated total of 6,641,960 women exposed during the study period had an average GBS isolation rate of 5.19 (5.14-5.25), geographical group rates being: Western Europe (2.2), North America (2.1), Australia (3.7), Spain (4.6), Latin America II (4.5), Eastern Europe (5.3), Asia (6.7), Latin America I (7.7), Middle East (7.9), Indian Subcontinent (17.2), North Africa (17.8), Sub-Saharan Africa (22.7). The 4532 pregnant women studied had an average GBS colonization rate of 12.47% (11.51-13.43) and geographical group rates varied similar to geographical isolation rates. Low GDP and high temperatures of the birth country were associated with higher colonization rates. Thus, differences in GBS colonization depend on the country of origin; Africa and the Indian subcontinent presented the highest, while Western Europe and North America had the lowest. This variability portrays a geographical pattern influenced by temperature and GDP.
Assuntos
Infecções Estreptocócicas/patologia , Streptococcus agalactiae/isolamento & purificação , Adulto , Emigrantes e Imigrantes , Etnicidade , Feminino , Humanos , Gravidez , Estudos Retrospectivos , Espanha/epidemiologia , Infecções Estreptocócicas/epidemiologia , Infecções Estreptocócicas/etnologia , Infecções Estreptocócicas/microbiologia , TemperaturaRESUMO
No disponible
Assuntos
Humanos , Feminino , Adulto , Doença Inflamatória Pélvica/diagnóstico , Doença Inflamatória Pélvica/microbiologia , Infecções Pneumocócicas/diagnóstico , Streptococcus pneumoniae , EndossonografiaRESUMO
No disponible
Assuntos
Humanos , Gonorreia/tratamento farmacológico , Farmacorresistência Bacteriana Múltipla , Neisseria gonorrhoeae/efeitos dos fármacos , Gonorreia/microbiologia , Neisseria gonorrhoeae/isolamento & purificação , Testes de Sensibilidade Microbiana , Hospitais Universitários , Antibacterianos/classificação , Antibacterianos/uso terapêutico , EspanhaRESUMO
El diagnóstico microbiológico de las infecciones causadas por micoplasmas y ureaplasmas se ha visto siempre limitado por el crecimiento muy dificultoso de estos microorganismos, la falta de medios de cultivo comercializados, la ausencia de procedimientos diagnósticos rápidos y la percepción clínica extendida de que estos microorganismos tienen una importancia menor en el contexto de las enfermedades infecciosas. Esta situación ha cambiado notablemente en los últimos años gracias a la comercialización de los medios de cultivo, al desarrollo de técnicas rápidas de diagnóstico serológico y, especialmente, por la aplicación de métodos de amplificación de ácidos nucleicos, comercializados o desarrollados en el propio laboratorio. Aunque se acusa la falta de estandarización y validación de las técnicas moleculares y serológicas, el avance en la metodología ha propiciado un aumento en su detección y, en consecuencia, en la apreciación de la importancia clínica de estos microorganismos (AU)
The microbiological diagnosis of mycoplasma and urea plasma infections has always been limited due to the fastidious growth of these microorganisms, as well as the lack of commercially prepared growth media, absence of rapid diagnostic procedures, and the clinical perception that these organisms are less significant in the infectious diseases setting. During the last few years, this situation has substantially improved due to the commercial availability of culture media, the development of rapid serological techniques, and, in particular, to the introduction of nucleic acid amplification assays, commercially available or "in-house" preparations. Despite the lack of proper standardisation and validation of the molecular and serological techniques, methodological advances have led to an increased detection of these microorganisms and, consequently, a greater appreciation of their clinical relevance (AU)