Influence of maize genotypes and harvest stages on in-silo fermentation quality and nutritional value of corn silage during hot summer condition of the tropics.

The aim of the experiment was to evaluate the potential of promising summer maize genotypes and optimal stage of harvesting these genotypes for ensiling in terms of dry matter (DM), starch, and crude protein (CP) yields, silage fermentation quality, nutrients profile, total digestible nutrients, metabolizable energy (ME) content, Cornell Net Carbohydrate and Protein System (CNCPS) carbohydrate (CHO) subfractions composition, in vitro DM digestibility (DMD) and in situ starch degradation characteristics. Six maize genotypes were chosen for the study: DK9108 from Monsanto, P30Y87, P3939 from Pioneer, QPM-300 (quality protein maize) and W94 from the International Maize and Wheat Improvement Center (CIMMYT), and a local cultivar, Afgoii, from the Cereal Research Institute (Persabaq, KP). A total of 72 plots (8 m × 10 m) were blocked in three replicate fields, and within each field, each genotype was sown in four replicate plots according to a randomized complete block design. For the data analysis, the Proc-Mixed procedure of Statistical Analysis System with repeated measure analysis of variance was used. The DM yield was strongly influenced (P < 0.001) by maize genotypes, varying from 12.6 to 17.0 tons/ha. Except for total CHO and ammonia nitrogen (NH3-N), the contents of all measured chemical components varied (P < 0.001) among the genotypes. Further comparison revealed that, genotype P3939 had a higher (P < 0.05) content of CP (7.27 vs. 6.92%), starch (36.7 vs. 27.9%), DMD (65.4 vs. 60.0%), ME (2.51 vs. 2.30 Mcal/kg) and lactic acid (5.32 vs. 4.83%) and lowest content of NDF (37.3 vs. 43.1%), pH (3.7 vs. 4.10) compared to the local cultivar (Afgoii). Advancement of post-flowering maturity from 25 to 35% DM (23 to 41 days after flowering (DAF)) increased (P < 0.05) the DM yield (10.4 to 17.8 tons/ha), starch content (29.1 to 35.0%), DMD (65.3 to 67.3%) and ME (2.34 to 2.47 Mcal/kg), and decreased (P < 0.001) the contents of CP (7.42-6.73%), NDF (48.8-38.5%), pH (4.10 to 3.60), NH3-N (8.93-7.80%N) and effective degradability of starch (95.4 to 89.4). Results showed that for higher yields and silage nutritional and fermentation quality, maize crops should be harvested at whole crop DM content of 30-35% (34 to 41 DAF). It was further concluded that genotype P3939 is the most suitable summer maize genotype for silage production in terms of yields and silage nutritional and fermentation quality under the hot environmental conditions of the tropics.

Immunomodulatory Activity in vitro and in vivo of Polysaccharides from Kabuli Chickpea (Cicer arietinum L.) Hull.

RESEARCH BACKGROUND: Polysaccharides isolated from plants, fungi and bacteria are associated with immunomodulatory effects. Chickpea hull, which is regarded as food industrial waste, contains considerable amount of antioxidants and bioactive compounds. EXPERIMENTAL APPROACH: In the present study, we investigated the immunomodulatory activity of polysaccharides from kabuli chickpea (Cicer arietinum L.) hull (CHPS). In vitro study was conducted with RAW264.7 cell line while in vivo study was carried out using specific pathogen-free BALB/c mouse animal model. RESULTS AND DISCUSSION: In in vitro test with RAW264.7 murine macrophage cells, the three purified fractions of chickpea hull polysaccharides showed potent immunomodulatory activity. Sample CHPS-3 showed stronger effect on cell viability, promoted the phagocytosis index to a greater extent and had the best effect on acid phosphatase activity. Moreover, it was found that CHPS-3 significantly (p<0.05) enhanced the secretion of nitrogen monoxide and cytokine (interleukins IL-6, IL-1ß and tumor necrosis factor-alpha (TNF-α)) levels. In in vivo study, CHPS-3 improved thymus and spleen indices in cyclophosphamide-induced immunodeficient mice. Increased activities of lysozyme, catalase, superoxide dismutase and glutathione peroxidase, serum haemolysin content and total antioxidant capacity were observed, while the amount of malondialdehyde in the liver decreased. NOVELTY AND SCIENTIFIC CONTRIBUTION: The results suggest that chickpea hull polysaccharides enhanced the immune activity and could be developed as the ingredient of functional foods.

Carboxymethyl cellulose based films enriched with polysaccharides from mulberry leaves (Morus alba L.) as new biodegradable packaging material.

The objective of this study was to determine the properties of a new active packaging film developed by the addition of mulberry leaves polysaccharides (MLP) into carboxymethyl cellulose (CMC). Biodegradable CMC-MLP films were fabricated by casting method with various concentrations of MLP (1, 5 and 10 % w/w). The addition of MLP into the CMC matrix resulted increased thickness (0.126 to 0.163 mm) and roughness of the films. Also, the decline in moisture content from 27.91 to 14.12 %, water vapor permeability from 8.95 to 5.21 × 10-10 g-1 s-1 Pa-1, and a swelling degree from 59.11 to 37.45 % were observed. With the increasing concentration of MLP, the mechanical properties of the films were improved and higher dispersion of UV light were noted. Fourier transform - infrared spectroscopy (FT-IR) and X-ray diffraction revealed good inter-molecular interaction between CMC matrix and MLP. The prepared films showed excellent thermal stability, antioxidant and antibacterial properties as well as susceptibility to biodegradation in the soil environment. Moreover, it was proved that the films have ability to retard oil oxidation. Overall, it was concluded that CMC-MLP films constitute a promising biomaterial that may be applied as active food packaging.

Investigating the structural properties and in vitro digestion of rice flours.

The physicochemical properties, swelling power, solubility, and digestibility of flour from four rice varieties (black, brown, white, and waxy rice flour) were analyzed. The results showed that the black and brown rice had high-amylose percentage (21.8% and 20.5%), a relatively low percentage of starch content (68.1% and 79.1%), and lower swelling power (6.6% and 7.6%) and solubility (13.5% and 15.7%), respectively. Waxy rice flour attributed to lower gelatinization temperatures and higher enthalpy values. Meanwhile, the brown, black, and white rice showed higher gelatinization temperature and lower enthalpy value. The black and brown rice flour exhibited lower pasting and viscosity values as compared to waxy rice flour. The results showed that all rice flour had an A-type X-ray diffraction pattern, and after cooking all rice flour showed V-type polymorphs except waxy rice flour. Brown and black rice flour after cooking have lower digestion rate than white rice and waxy rice flour, probably due to its lower expansion and solubility rates, and higher gelatinization temperature.

Antioxidant and anti-inflammatory activities of target anthocyanins di-glucosides isolated from Syzygium cumini pulp by high speed counter-current chromatography.

High-speed counter-current chromatography (HSCCC) was utilized as an effective procedure for isolation of targeted three anthocyanins di-glucosides from Syzygium cumini (SC) pulp by using an optimized biphasic successful combination of different solvents. The resulted fractions described by HPLC/ESI-MS to be delphinidin 3,5-diglucoside (DDG), petunidin 3,5-diglucoside (PDG), and malvidin 3,5-diglucoside (MDG). A weight of 150 mg of sample yielded 7.53, 22.68, and 39.09 mg for DDG, PDG, and MDG, respectively. It was stated that the target three anthocyanins possessed strong antioxidant activities. Furthermore, MDG exhibited definite advantages for inhibition of nitric oxide release and pro-inflammatory mediators like mouse interleukin 6 (IL-6), mouse interleukin (IL-1ß) and mouse tumor necrosis factor (TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. The results propose that HSCCC can be utilized to separate highly antioxidative and anti-inflammatory natural components from SC pulp. PRACTICAL APPLICATIONS: Anthocyanins are water-soluble pigments and considered one of the groups of bioactive compounds, which generally concentrate in the skin, and often also the flesh of some fruits and vegetables as glycosides like acylglycosides and aglycones of anthocyanidins. The fully ripe fruits of SC contain anthocyanins, like as delphinidin, cyanidin, and petunidin, which supply them a distinctive color and good antioxidant characteristics. HSCCC considers a system of liquid-liquid extraction with separating large quantities of materials, using a minimum of solvent. The findings of the study would pave a separation strategy for potential large-scale preparation of anthocyanins di-glucosides standards for compounds detection and reduce the inflammation symptoms through declining the induction of pro-inflammatory cytokines such as IL-1ß, TNF-α and IL-6, which will also enhance the future notification on the structure-activity correlations of anthocyanins di-glucosides.

Production and characterization of CMC-based antioxidant and antimicrobial films enriched with chickpea hull polysaccharides.

In the present study, polysaccharides from chickpea hull (CHPS) were incorporated into carboxymethyl cellulose (CMC) for the development of CMC-based films. The physical and mechanical properties, color, transmittance, antioxidant and antimicrobial activities were investigated, and differential scanning calorimetry (DSC), Fourier transform-infrared (FT-IR) spectroscopy and thermogravimetric analysis (TGA) were applied to study the potential interaction, structure and thermal stabilization of the prepared films. The results revealed that some physical and mechanical properties of films, like moisture content, elongation at break and water vapor permeability, were decreased. While thickness, swelling ratio, water solubility and tensile strength were significantly increased (p < 0.05) compared with control film. Furthermore, the films exhibited potential antioxidant effects on DPPH and ABTS free radicals. The results of scanning electron microscopy showed rough and heterogeneous morphology for CMC-CHPS films while control film exhibited smooth, homogenous and compact structure. FT-IR results reflected good interaction of chemical groups and bonds between CMC and CHPS. DSC results showed that glass transition temperature increased significantly (p < 0.05) from 82.68 to 90.39 °C compared with control of 78.21 °C. Thermal stability of all films was improved, indicating that the films could be used as biocomposite materials for packaging of food products.

Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.

In the present study, apple peel polyphenols (APP) were incorporated into chitosan (CS) to develop a novel functional film. Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films. Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured. The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased. Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film. Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased. CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.