RESUMO
Feather analysis has been widely used as a biomonitoring tool to assess metal contamination in birds, as their sampling is a non-destructive and ethically preferable technique. However, for feathers to be useful as a biomonitor of heavy metals, exogenous contamination has to be efficiently removed. Although much effort has been put into this, no washing procedure has yet proven able to ensure the total removal of the surface-associated metals. The purpose of this study was to propose an efficient washing procedure of feather samples prior to metal analysis, on the basis of comparison of various washing schemes designed according to previous analytical trials, and of the verification of the efficacy of the optimal scheme in cleaning intentionally contaminated feathers. Our investigation showed that dilute nitric acid alone or in combination with a detergent (Extran) or acetone under mild agitation of the samples performed better that any other cleaning scheme applied. Thus, a multi-step procedure including the sequential use of all three reagents was tested against feather samples contaminated by adsorbed or particulate metal species. The procedure was able to completely eliminate the external metal loads in all cases except for the partial removal of severe contamination with adsorbed Cd.