RESUMO
BACKGROUND: Sudanese children with congenital heart defects (CHDs) were found to have poorer oral health than those without CHDs. The aims of this study were to: describe the patterns of oral-health-related background factors in children with and without CHD and explore any differences, and to evaluate the effects of background factors on caries and gingivitis prevalence and dental services utilisation. METHODS: In this analytical cross-sectional study, caregivers of children aged 3-12 years with (CHD cases n = 111) and without CHDs (Controls n = 182), underwent face-to-face interviews using a structured questionnaire. The questionnaire items covered several oral health background factors (independent variables) including: child's health status, oral hygiene practices, dental services utilization, mother's level of education, and caregiver's perception and awareness of their child's oral health. The relationship between these factors and occurrence of 'caries' and 'gingivitis' as well as 'child's dental services utilisation' (dependent variables) were explored using multiple adjusted and hierarchal logistic regression analyses. RESULTS: Compared with controls, CHD cases had lower frequencies of brushing and use of fluoridated toothpaste, and their caregivers were less knowledgeable about caries. Among CHD cases, the variables (brushing and fluoridated toothpaste use) had significant impacts on caries prevalence (odd ratio (OR) =5.6, 95% confidence interval (CI): 1.4-22.8 and OR = 0.3, 95% CI: 0.1-0.8 for infrequent compared to frequent ones, respectively) as well as the mother's level of education (OR = 2.6, 95% CI: 1.0-6.4). When differences in background factors were controlled for, the adjusted ORs for caries and gingivitis prevalence in CHD cases compared with controls were 1.8, (95% CI: 1.1-3.2) and 5.3 (95% CI: 2.9-9.4), respectively. Among CHD cases, the child's age (8-12 years: OR = 11.9, 95% CI: 1.9-71.6), and the mother's level of education (lower education: OR = 0.2, 95% CI: 0.03-0.9) were significantly associated with the child's dental services utilisation. CONCLUSIONS: Lower frequencies of brushing and use of fluoride tooth paste were reported among CHD cases, and brushing had the predominant significant impact on caries prevalence. The child's age and the mother's level of education were the main factors affecting the child's (CHD cases) dental services utilisation.
Assuntos
Assistência Odontológica , Cárie Dentária/complicações , Cardiopatias Congênitas/complicações , Saúde Bucal , Criança , Pré-Escolar , Estudos Transversais , Feminino , Humanos , Masculino , PrevalênciaRESUMO
BACKGROUND AND OBJECTIVE: Aggressive periodontitis (AgP) is prevalent and shows a rapid course in African individuals. Although a strong focus has been placed on Aggregatibacter actinomycetemcomitans, new methods support the existence of a complex subgingival microflora in AgP. The purpose of the present study was to map the subgingival microbiota as well as explore the presence of A. actinomycetemcomitans and the JP2 clone in a group of Sudanese individuals with AgP, using different analytical methods. MATERIAL AND METHODS: A study population consisting of 19 patients with AgP was recruited from patients seeking treatment at University of Science and Technology (UST) in Khartoum. Fifteen healthy subjects were included as controls. Plaque samples were analyzed for 272 taxa using human oral microbe identification microarrays and for 26 periodontal taxa using DNA-DNA hybridization checkerboard. Conventional polymerase chain reaction (PCR) was applied for the detection of A. actinomycetemcomitans and the JP2 clone in plaque. Saliva from patients with AgP was analyzed using quantitative PCR (qPCR) for the detection of A. actinomycetemcomitans. RESULTS: Eubacterium yurii was detected more frequently in patients with AgP than in controls, and E. nodatum was found in patients with AgP only. A. actinomycetemcomitans was found in plaque samples of two (12%) patients by human oral microbe identification microarrays and in five (29%) patients with AgP by conventional PCR, as well as in six (32%) of the AgP saliva samples by qPCR. The JP2 clone was identified in only one patient. CONCLUSION: The classical periodontal pathogens were not present in high amounts in AgP in the population studied here. Species of Eubacterium, which are not typically associated with AgP, were often detected in individuals with disease. Using laboratory methods with different sensitivities and detection levels allowed identification of variances in microbial communities. The findings reported in this study provide a basis for the further understanding of AgP.
Assuntos
Periodontite Agressiva , Aggregatibacter actinomycetemcomitans , Placa Dentária , Eubacterium , Humanos , Reação em Cadeia da Polimerase em Tempo RealRESUMO
BACKGROUND AND OBJECTIVE: Aggregatibacter actinomycetemcomitans is considered a possible etiological agent for aggressive periodontitis. The aim of this study was to determine the prevalence of the JP2 clone and non-JP2 genotypes of A. actinomycetemcomitans in the subgingival plaque of patients with aggressive periodontitis and controls among Sudanese high-school students. MATERIAL AND METHODS: In a previous study we examined a large representative sample of students attending high schools in Khartoum, Sudan. In this population, 17 patients with aggressive periodontitis and 17 controls (14-19 years of age) consented to participate in the present study. The subjects underwent a clinical periodontal examination, and subgingival dental plaque samples were collected using paper points. The presence of the A. actinomycetemcomitans JP2 clone and non-JP2 genotypes were assessed using loop-mediated isothermal amplification (LAMP) and the PCR. RESULTS: The JP2 clone of A. actinomycetemcomitans was not detected in the subgingival plaque of either the cases or the controls. Non-JP2 types of A. actinomycetemcomitans were detected in the subgingival plaque of 12 (70.6%) patients with aggressive periodontitis and from only one (5.9%) control subject, showing a significantly higher frequency of detection in cases than in controls (p = 0.0001). The odds ratio for the detection of A. actinomycetemcomitans in the subgingival plaque of the patients with aggressive periodontitis was 38.4 (95% confidence interval: 4.0-373.0; p = 0.002). The PCR and LAMP methods showed identical results pertaining to the identification of non-JP2 types of A. actinomycetemcomitans. CONCLUSIONS: The JP2 clone of A. actinomycetemcomitans was not detected in the subgingival plaque of high school subjects in Sudan. The detection of non-JP2 types of A. actinomycetemcomitans may be a useful marker of increased risk for development of aggressive periodontitis in young subjects.
Assuntos
Infecções por Actinobacillus/microbiologia , Aggregatibacter actinomycetemcomitans/genética , Periodontite Agressiva/microbiologia , Adolescente , Aggregatibacter actinomycetemcomitans/classificação , Toxinas Bacterianas/genética , Estudos de Casos e Controles , Células Clonais , DNA Bacteriano/análise , Placa Dentária/microbiologia , Exotoxinas/genética , Feminino , Genótipo , Humanos , Masculino , Técnicas de Amplificação de Ácido Nucleico , Reação em Cadeia da Polimerase , RNA Bacteriano/análise , RNA Ribossômico 16S/análise , Sudão , Adulto JovemRESUMO
The aim of this study was to estimate the prevalence of teeth with apical periodontitis (AP) and root-filled (RF) teeth in an adult Sudanese population. Panoramic and periapical radiographs were obtained for 200 patients over 18 years of age seeking routine dental care and attending the dental clinics (University of Khartoum) and the dental hospital (University of Science and Technology) for the first time. The periapical status of all teeth (except third molars) was categorized on the basis of the presence or absence of radiographic signs of AP. In addition, the frequency of RF teeth was recorded. Data were analyzed using the chi-square test and odds ratio (OR). The periapical status of 4,967 teeth was assessed. AP in one or several teeth was identified in 95 (47%) patients and in 3.3% of the teeth. The prevalence of AP was higher in molar teeth (7.3%) than in premolar (3.5%) and anterior teeth (0.9%, p ≤ .001). There were 80 (1.6%) RF teeth in 42 (21%) patients. The probability of root-filling in molar and premolar teeth was almost twice that of anterior teeth (OR with 95% confidence intervals: 1.06 < 1.91 < 3.44, p ≤ .05). The prevalence of RF teeth increased with age (OR of 48 ± year = 3.06, p ≤ .001). Statistical analysis showed that the probability of radiological detection of AP in RF teeth was 17-fold higher than in nonfilled ones (OR with 95% confidence intervals: 9.87 < 16.83 < 28.25, p ≤ .001). Therefore, the probability of AP, RF teeth with or without AP, and missing teeth was high in molar teeth than in anterior or premolar teeth. The frequency of RF teeth was low compared to that demonstrated in most other epidemiological studies. This highlights the need to focus on improving the quality of restorations and the procedure by which root canal is shaped and disinfected.
RESUMO
This study aimed to examine early posttreatment changes in the periodontal microflora. Paper point sampling and conventional bacterial cultivation were used to monitor the effects of surgical and non-surgical periodontal therapy on the detection frequency of Porphyromonas gingivalis, Prevotella intermedia, Actinobacillus actinomycetemcomitans, and Capnocytophaga species in deep periodontal pockets. Ten patients, 5 men and 5 women (mean age 44 years), with advanced periodontal disease were selected from the dental school patient population for the study. A total of 245 teeth in 10 defined areas of the dentition were treated by oral hygiene instruction followed by scaling and root planing alone (121 sites) or with surgical interventions (124 sites). Ninety sites, 47 surgical and 43 non-surgical, with initial pocket depth greater than or equal to 6 mm were sampled at baseline and 3 months after completion of therapy. Treatment by both procedures resulted in significant clinical improvements as assessed by all clinical parameters used. Baseline results may indicate that the level of P. gingivalis was reduced in the presence of P. intermedia, while A. actinomycetemcomitans seemed to be reduced in the presence of P. gingivalis and/or P. intermedia. Three months after therapy, the detection frequency of P. gingivalis was significantly reduced (P less than 0.05) in surgical and non-surgical sites while the reduction for P. intermedia was significant only for surgical sites (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Bactérias/isolamento & purificação , Periodontite/terapia , Adulto , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Capnocytophaga/isolamento & purificação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Índice Periodontal , Bolsa Periodontal/microbiologia , Bolsa Periodontal/terapia , Periodontite/microbiologia , Fatores de TempoRESUMO
Twenty-five sudanese and 18 norwegian adult periodontitis patients were selected to participate in this study. The purpose was to compare cultivation results of Porphyromonas gingivalis, Prevotella intermedia, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, and Capnocytophaga species as well as various enteric rods in both populations. In addition, DNA probe analysis was used to identify P. gingivalis, P. intermedia, A. actinomycetemcomitans, F. nucleatum, and Bacteroides forsythus in the Sudanese patients and results were compared with those obtained by cultivation. The paper point technique was used to sample 99 sites in the Sudanese group (4 paper points/site) and 119 sites in the Norwegian patients (3 paper points/site). In the Sudanese subjects, the fourth paper point was used for the DNA probe analysis. The chi-square test and the Wilcoxon signed rank test were used to test for statistically significant differences between Sudanese and Norwegian cultivation results as well as between cultivation and DNA results in the Sudanese group. Cultivation results indicated that the Sudanese subjects had significantly lower prevalence of P. gingivalis, P. intermedia, and F. nucleatum (P < 0.01), significantly higher prevalence of Capnocytophaga species (P < 0.05), and similar prevalence of A. actinomycetemcomitans. Almost all Sudanese subjects tested positive for various enteric rods, while none of the Norwegians did so. The extent to which unrestricted use of antibiotics and transport media influenced the levels of enteric species is not known, however.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Periodontite/microbiologia , Adulto , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Capnocytophaga/isolamento & purificação , Distribuição de Qui-Quadrado , Contagem de Colônia Microbiana , Sondas de DNA , DNA Bacteriano/análise , Enterobacteriaceae/isolamento & purificação , Feminino , Fusobacterium nucleatum/isolamento & purificação , Humanos , Masculino , Noruega/epidemiologia , Índice Periodontal , Bolsa Periodontal/microbiologia , Periodontite/etnologia , Porphyromonas gingivalis/isolamento & purificação , Prevalência , Prevotella intermedia/isolamento & purificação , Estatísticas não Paramétricas , Sudão/epidemiologiaRESUMO
The few previous cultivation studies on the in vivo associations between various periodontal microbial species have shown several positive and negative associations. The present investigation utilized DNA probe analysis to examine possible in vivo associations between the periodontal pathogens Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Bacteroides forsythus in subgingival plaque samples obtained from 25 Sudanese untreated adult periodontitis patients. The standard paper point technique was used to sample 99 sites with a mean probing depth of 6.8 mm (range 6.0 to 10.0). Microbial associations were determined by detecting the effect of the presence or absence of one species (effector) on the occurrence of the other 3 species (target). The Wilcoxon signed rank test was used to examine variations in occurrence of each target bacteria in the presence or absence of the effector. In addition, the Spearman's rank correlation test was used to assess the relationship between the level of each bacteria to that of the other 3. Results showed bacterial associations with the following effector-on-target effects: F. nucleatum (P < 0.01 Wilcoxon; P < 0.001 Spearman) >> P. gingivalis (P < 0.01 Wilcoxon; P < 0.001 Spearman), and B. forsythus (P < 0.05 Wilcoxon; P < 0.001 Spearman) > P. intermedia (P < 0.01 Spearman). The study demonstrated positive associations between the 4 species investigated, while no neutral or negative associations were revealed. The most striking finding was the effect exerted by F. nucleatum on the colonization of P. intermedia; P. intermedia was never detected in a site unless F. nucleatum was also present.
Assuntos
Bactérias Anaeróbias/fisiologia , Periodontite/microbiologia , Adulto , Bactérias Anaeróbias/isolamento & purificação , Bacteroides/isolamento & purificação , Bacteroides/fisiologia , Contagem de Colônia Microbiana , Sondas de DNA , DNA Bacteriano/análise , Placa Dentária/microbiologia , Ecossistema , Feminino , Fusobacterium nucleatum/isolamento & purificação , Fusobacterium nucleatum/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Bolsa Periodontal/microbiologia , Porphyromonas gingivalis/isolamento & purificação , Porphyromonas gingivalis/fisiologia , Prevotella intermedia/isolamento & purificação , Prevotella intermedia/fisiologia , Estatísticas não Paramétricas , SimbioseRESUMO
The aim of this study was to determine the survival in VMGA III of Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and enteric rods in laboratory cultures as well as in subgingival plaque samples. Laboratory strains of the 4 putative periodontal pathogens and Escherichia coli were used in the laboratory part of this study. Also, 31 subgingival plaque samples were obtained from 22 periodontal patients and stored in VMGA III. Each sample, from both the laboratory and the clinical parts, was divided into 3 portions. One portion was cultured within a few hours of collection (baseline), while the second was processed after 24 h (day 2) and the third 48 h later (day 3). The results of the clinical part indicate that the detection frequencies of all 4 periodontal pathogens and their levels in positive samples decreased, to different degrees, by day 2 and decreased further by day 3. Enteric rods were not detected in base line samples. However, they were present in 16.1% and 22.6% of day 2 and day 3 samples, respectively. Similarly, the laboratory results demonstrate a significant decrease in the levels of the 4 periodontal pathogens tested by day 2 and day 3, whereas the opposite occurred for E. coli. P. gingivalis, P. intermedia, and F. nucleatum survived better in the presence of E. coli than alone, whereas A. actinomycetemcomitans survived less well when co-inoculated with E. coli. VMGA III appears to maintain microbial population ratios for periods up to 24 h. After 24 h, the multiplication of enteric organisms may alter the original proportions of the sample.
Assuntos
Bactérias Anaeróbias/crescimento & desenvolvimento , Meios de Cultura , Placa Dentária/microbiologia , Preservação Biológica/métodos , Aggregatibacter actinomycetemcomitans/crescimento & desenvolvimento , Anaerobiose , Divisão Celular , Contagem de Colônia Microbiana , Escherichia coli/crescimento & desenvolvimento , Fusobacterium nucleatum/crescimento & desenvolvimento , Humanos , Compostos Orgânicos , Doenças Periodontais/microbiologia , Porphyromonas gingivalis/crescimento & desenvolvimento , Prevotella intermedia/crescimento & desenvolvimento , Estatísticas não Paramétricas , Fatores de TempoRESUMO
Porphyromonas gingivalis clonal types that participate in periodontal infections express serologically distinct surface antigens. This investigation sought to determine whether serum antibodies titers against the serotype-specific capsular carbohydrate K antigen and lipopolysaccharide antigens of P. gingivalis might reveal which serotypes are most likely to be responsible for subgingival infections in subjects with adult periodontitis. Immunoglobulin G (IgG) titers to purified K antigen and lipopolysaccharide from different P. gingivalis strains were measured by ELISA for 28 healthy controls and 51 patients with periodontal pockets known to be infected with genetically and serologically distinct P. gingivalis clonal types. Titers to purified K antigen from strains W50, HG184, A7A1-28, 49417, HG1690 and HG1691, representing serotypes K1-K6, respectively, and lipopolysaccharide from strains 381, HG1691 and W50, representing serotypes O1-O3, respectively, were measured for all subjects. Chi-square likelihood ratios, Mann-Whitney tests and receiver-operating characteristic sensitivity-specificity plots were used to compare the accuracy with which titer results for different target antigens classified subjects with or without disease. Results from assays targeting K2, K3, K4, K5, O1 and O2 generally gave poor diagnostic accuracy, whether evaluated separately or as summed titer pairs corresponding to the K/O combinations actually expressed by the target antigen parent strains. Exceptions were O3 (from W50) and K5+O2 (both from HG1690), which gave moderate accuracy in classifying subjects. In contrast, highly significant diagnostic accuracy was achieved using individual K1 (W50) and K6 (HG1691) titer data and K1+O3 (W50) and K6+O2 (HG1691) titer sum values. These observations suggest that P. gingivalis clonal types expressing K/O serotypes matching those of W50 (K1/O3) and HG1691 (K6/O2) are more likely than others to participate in periodontal infections in adult periodontitis patients and thus are more likely than others to express relevant virulence factors.
Assuntos
Antígenos de Bactérias/imunologia , Cápsulas Bacterianas/imunologia , Imunoglobulina G/imunologia , Lipopolissacarídeos/imunologia , Periodontite/imunologia , Porphyromonas gingivalis/imunologia , Adulto , Antígenos de Superfície/imunologia , Distribuição de Qui-Quadrado , Ensaio de Imunoadsorção Enzimática , Epitopos/imunologia , Humanos , Imunoglobulina G/análise , Funções Verossimilhança , Antígenos O/imunologia , Bolsa Periodontal/imunologia , Bolsa Periodontal/microbiologia , Periodontite/microbiologia , Polissacarídeos Bacterianos/imunologia , Porphyromonas gingivalis/classificação , Porphyromonas gingivalis/genética , Curva ROC , Sensibilidade e Especificidade , Sorotipagem , Estatísticas não Paramétricas , VirulênciaRESUMO
The aim of the present study was to determine by standard cultivation procedures the detection frequencies of Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Capnocytophaga species as well as various enteric rods in subgingival plaque samples form Romanian adult periodontitis patients. DNA probe analysis (Affirm DP Microbial Identification Test) was also used, parallel to cultivation, to identify P. gingivalis, A. actinomycetemcomitans, and B. forsythus, in deep (> or = 6 mm) and intermediate (4-5 mm) pockets in some of the subjects investigated. Paper points were used to sample 86 deep pockets in 36 patients and 27 intermediate pockets in 9 of the 36 patients. The chi 2 test was used to test for significance of differences between results obtained by cultivation and DNA analysis in both intermediate and deep pockets. P. gingivalis was recovered in a high percentage of the patients (75.8%) and sites (63.6%) examined, followed by P. intermedia, F. nucleatum, and A. actinomycetemcomitans, respectively. Capnocytophaga species were present in almost all subjects. Enteric rods were recovered in 61.1% of the patients and 55.8% of the sites. Except for this high prevalence of enteric rods, the present group of patients had the periodontal species monitored in %s similar to those commonly perceived in the West. The Affirm DP Test and cultivation showed poor correlation in detecting P. gingivalis, A. actinomycetemcomitans, and B. forsythus. The cultivation prevalence of P. gingivalis and P. intermedia in deep pockets was similar to their prevalence in intermediate ones. Overall, the prevalence of the periodontal pathogens investigated in the present Romanian periodontitis patients is similar to what has been revealed in matching Norwegian and other Western periodontitis patient populations. The high prevalence of enteric rods in the Romanian patients may have been an artifact resulting from prolonged transport of the samples in VMGA III.
Assuntos
Bactérias/isolamento & purificação , Placa Dentária/microbiologia , Periodontite/microbiologia , Adulto , Idoso , Aggregatibacter actinomycetemcomitans/genética , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Artefatos , Bactérias/classificação , Técnicas Bacteriológicas , Bacteroides/genética , Bacteroides/isolamento & purificação , Capnocytophaga/genética , Capnocytophaga/isolamento & purificação , Contagem de Colônia Microbiana , Meios de Cultura , Sondas de DNA , DNA Bacteriano/análise , Enterobacteriaceae/genética , Enterobacteriaceae/isolamento & purificação , Feminino , Fusobacterium nucleatum/genética , Fusobacterium nucleatum/isolamento & purificação , Gengiva/microbiologia , Humanos , Masculino , Pessoa de Meia-Idade , Bolsa Periodontal/microbiologia , Porphyromonas gingivalis/genética , Porphyromonas gingivalis/isolamento & purificação , Prevalência , Prevotella intermedia/genética , Prevotella intermedia/isolamento & purificação , Romênia , Manejo de EspécimesRESUMO
Porphyromonas gingivalis has been isolated from periodontitis lesions in subjects from many geographical locations. The purpose of this investigation was to determine whether similar ribotypes of P. gingivalis could be detected among strains isolated in different countries. A total of 198 isolates of P. gingivalis were obtained from 52 periodontitis patients in Boston (130 isolates), Bergen, Norway (17 isolates), Khartoum, Sudan (26 isolates), and Bucharest, Romania (25 isolates). DNA was isolated from each strain, cut separately by the restriction endonucleases KpnI and PstI. The resulting preparations were subjected to electrophoresis in a 0.8% agarose gel using a Tris-acetate EDTA buffer. Uncut lambda and a 1000-bp fragment of 16S rRNA were included as internal standards in each lane. In addition, a HindIII digest of lambda was present in a separate lane in each run. The DNA fragments were transferred to a nylon membrane by downward capillary transfer. 16S rRNA bands were detected using a 1000-kb digoxigenin-labelled probe generated by a polymerase chain reaction. At the same time, a digoxigenin-labelled probe to lambda was employed to detect the internal and molecular weight standards. The bands were detected using antibody to digoxigenin conjugated to alkaline phosphatase and chemiluminescence. The positions of the bands relative to the internal standards were determined and normalized to correct for run-to-run variations, and the molecular weight of each band was determined by comparison with standards within each gel. The resulting data for the 2 enzymes were combined and subjected to cluster analysis using an average unweighted linkage sort. In some instances, isolates that appeared to be of identical ribotype using one endonuclease gave different ribotypes using the other. Strains of P. gingivalis within a subject were usually identical, except for 3 patients who harbored 2 different ribotypes/individual. All subsequent analyses employed a single ribotype strain for each subject. A total of 32 ribotypes were observed for isolates from distant countries. A total of 11.5% of the patients had isolates exhibiting the same ribotype: ribotype 7a. Identical ribotypes of P. gingivalis can be recovered from subgingival plaque samples of periodontitis patients in different countries.
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Periodontite/microbiologia , Porphyromonas gingivalis/genética , Técnicas de Tipagem Bacteriana , Análise por Conglomerados , DNA Ribossômico/genética , Heterogeneidade Genética , Genótipo , Humanos , Epidemiologia Molecular , Noruega/epidemiologia , Periodontite/epidemiologia , Porphyromonas gingivalis/classificação , RNA Ribossômico 16S/análise , Romênia/epidemiologia , Sudão/epidemiologia , Estados Unidos/epidemiologiaRESUMO
Our study is the 1st report on subgingival microbiota in adult Cameronians. The aim was to investigate, using the checkerboard DNA-DNA hybridization technique, the prevalence of 18 oral species in subgingival plaque samples obtained from sex- and age-matched Cameronian adults with and without periodontal destruction. We also compared cultivation and the Affirm DP test with the checkerboard technique in their capability to detect some selected species among the 18. 21 adult periodontitis patients and 21 periodontally healthy subjects were examined and the results were compared statistically. Each periodontitis patient had at least 4 pockets of > or = 6 mm depth, while the healthy subjects had no sites more than 3 mm deep. Results of the checkerboard analysis showed that significantly (p < 0.05) more periodontitis patients tested positive for most of the 18 bacterial species. The Gram-positive species Actinomyces naeslundii, Streptococcus mitis and Streptococcus sanguis, known as microbiota of healthy sites, were detected significantly more frequently in the healthy group. Cultivation demonstrated P. gingivalis, B. forsythus, A. actinomycetemcomitans, P. intermedia and F. nucleatum in significantly lower %s of patients as compared to the checkerboard technique. Furthermore, the Affirm DP test detected P. gingivalis and B. forsythus in significantly fewer patients than did the checkerboard technique. A. actinomycetemcomitans was detected in 52.3% of the patients by the latter technique while the Affirm DP test failed to detect the bacterium in any of the samples. Overall, the results of the present study confirm the importance of the screening method and indicate that the prevalences of the investigated putative periodontal pathogens and beneficial species in the healthy and diseased adult Cameronians were similar to those reported for adults in the West and in some developing countries.
Assuntos
Bactérias/isolamento & purificação , Gengiva/microbiologia , Periodontite/microbiologia , Periodonto/microbiologia , Actinomyces/genética , Actinomyces/isolamento & purificação , Adulto , Aggregatibacter actinomycetemcomitans/genética , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Bactérias/classificação , Bactérias/genética , Técnicas Bacteriológicas , Bacteroides/genética , Bacteroides/isolamento & purificação , Camarões , Estudos de Casos e Controles , DNA Bacteriano/análise , Países Desenvolvidos , Países em Desenvolvimento , Feminino , Fusobacterium nucleatum/genética , Fusobacterium nucleatum/isolamento & purificação , Hemorragia Gengival/microbiologia , Humanos , Masculino , Pessoa de Meia-Idade , Hibridização de Ácido Nucleico , Bolsa Periodontal/microbiologia , Porphyromonas gingivalis/genética , Porphyromonas gingivalis/isolamento & purificação , Prevalência , Prevotella intermedia/genética , Prevotella intermedia/isolamento & purificação , Streptococcus/genética , Streptococcus/isolamento & purificação , Streptococcus sanguis/genética , Streptococcus sanguis/isolamento & purificaçãoRESUMO
We obtained clinical isolates of Porphyromonas gingivalis of known ribotype from patients diagnosed with adult periodontitis and used Western blot methodology to evaluate profiles of antigens recognized by IgG in heterologous and homologous patient sera. Our aims were to identify isolates belonging to different serogroups, to learn if serogroup membership is related to ribotype to assess variation in IgG responses of patients to antigens is homologous and heterologous ribotypes, and to determine the frequency of shared and variable antigens in different biochemical classes recognized across different serogroups and ribotypes. Blots of separation patterns of 28 isolates were developed in sera from patients and bound IgG was quantified by digital image densitometry. The membership of isolates in different serogroups was determined by correlation and hierarchical cluster analysis of isolate whole-cell IgG binding profiles. Two major isolate clusters, each with two subclusters, were found. Isolates within the same ribotype clustered together in some cases but not others. Homologous isolates ranked high in IgG binding levels relative to those from different patients irrespective of ribotype. Patient subgroups with IgG responses dominant for different ribotypes and serogroups were revealed by correlation analysis. The IgG binding profiles observed for individual protein and proteinase-resistant antigens across both homologous and heterologous isolates were very dissimilar. Furthermore, the frequency of antigens both shared across all ribotypes and recognized by IgG in patient sera was unexpectedly low. Only two protein antigens (Mr 44 kDa and 27 kDa) were strongly recognized across all ribotypes by different sera. We conclude that the IgG response of patients infected with a particular P. gingivalis serotype or ribotype is directed mainly against antigens that are not shared by other potentially infective clonal types.