Artificial intelligence models for validating and predicting the impact of chemical priming of hydrogen peroxide (H2O2) and light emitting diodes on in vitro grown industrial hemp (Cannabis sativa L.).

Industrial hemp (Cannabis sativa L.) is a highly recalcitrant plant under in vitro conditions that can be overcome by employing external stimuli. Hemp seeds were primed with 2.0-3.0% hydrogen peroxide (H2O2) followed by culture under different Light Emitting Diodes (LEDs) sources. Priming seeds with 2.0% yielded relatively high germination rate, growth, and other biochemical and enzymatic activities. The LED lights exerted a variable impact on Cannabis germination and enzymatic activities. Similarly, variable responses were observed for H2O2 × Blue-LEDs combination. The results were also analyzed by multiple regression analysis, followed by an investigation of the impact of both factors by Pareto chart and normal plots. The results were optimized by contour and surface plots for all parameters. Response surface optimizer optimized 2.0% H2O2 × 918 LUX LEDs for maximum scores of all output parameters. The results were predicted by employing Multilayer Perceptron (MLP), Random Forest (RF), and eXtreme Gradient Boosting (XGBoost) algorithms. Moreover, the validity of these models was assessed by using six different performance metrics. MLP performed better than RF and XGBoost models, considering all six-performance metrics. Despite the differences in scores, the performance indicators for all examined models were quite close to each other. It can easily be concluded that all three models are capable of predicting and validating data for cannabis seeds primed with H2O2 and grown under different LED lights.

Artificial neural network modeling for deciphering the in vitro induced salt stress tolerance in chickpea (Cicer arietinum L).

Salt stress is one of the most critical abiotic stresses having significant contribution in global agriculture production. Chickpea is sensitive to salt stress at various growth stages and a better knowledge of salt tolerance in chickpea would enable breeding of salt tolerant varieties. During present investigation, in vitro screening of desi chickpea by continuous exposure of seeds to NaCl-containing medium was performed. NaCl was applied in the MS medium at the rate of 6.25, 12.50, 25, 50, 75, 100, and 125 mM. Different germination indices and growth indices of roots and shoots were recorded. Mean germination (%) of roots and shoots ranged from 52.08 to 100%, and 41.67-100%, respectively. The mean germination time (MGT) of roots and shoots ranged from 2.40 to 4.78 d and 3.23-7.05 d. The coefficient of variation of the germination time (CVt) was recorded as 20.91-53.43% for roots, and 14.53-44.17% for shoots. The mean germination rate (MR) of roots was better than shoots. The uncertainty (U) values were tabulated as 0.43-1.59 (roots) and 0.92-2.33 (shoots). The synchronization index (Z) reflected the negative impact of elevated salinity levels on both root and shoot emergence. Application of NaCl exerted a negative impact on all growth indices compared to control and decreased gradually with elevated NaCl concentration. Results on salt tolerance index (STI) also revealed the reduced STI with elevated NaCl concentration and STI of roots was less than shoot. Elemental analysis revealed more Na and Cl accumulation with respective elevated NaCl concentrations. The In vitro growth parameters and STI values validated and predicted by multilayer perceptron (MLP) model revealed the relatively high R 2 values of all growth indices and STI. Findings of this study will be helpful to broaden the understanding about the salinity tolerance level of desi chickpea seeds under in vitro conditions using various germination indices and seedling growth indices. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-023-01282-z.

A unified framework of response surface methodology and coalescing of Firefly with random forest algorithm for enhancing nano-phytoremediation efficiency of chromium via in vitro regenerated aquatic macrophyte coontail (Ceratophyllum demersum L.).

Nano-phytoremediation is a novel green technique to remove toxic pollutants from the environment. In vitro regenerated Ceratophyllum demersum (L.) plants were exposed to different concentrations of chromium (Cr) and exposure times in the presence of titania nanoparticles (TiO2NPs). Response surface methodology was used for multiple statistical analyses like regression analysis and optimizing plots. The supplementation of NPs significantly impacted Cr in water and Cr removal (%), whereas NP × exposure time (T) statistically regulated all output parameters. The Firefly metaheuristic algorithm and the random forest (Firefly-RF) machine learning algorithms were coalesced to optimize hyperparameters, aiming to achieve the highest level of accuracy in predicted models. The R2 scores were recorded as 0.956 for Cr in water, 0.987 for Cr in the plant, 0.992 for bioconcentration factor (BCF), and 0.957 for Cr removal through the Firefly-RF model. The findings illustrated superior prediction performance from the random forest models when compared to the response surface methodology. The conclusion is drawn that metal-based nanoparticles (NPs) can effectively be utilized for nano-phytoremediation of heavy metals. This study has uncovered a promising outlook for the utilization of nanoparticles in nano-phytoremediation. This study is expected to pave the way for future research on the topic, facilitating further exploration of various nanoparticles and a thorough evaluation of their potential in aquatic ecosystems.

Artificial intelligence-based approaches to evaluate and optimize phytoremediation potential of in vitro regenerated aquatic macrophyte Ceratophyllum demersum L.

Water bodies or aquatic ecosystem are susceptible to heavy metal accumulation and can adversely affect the environment and human health especially in underdeveloped nations. Phytoremediation techniques of water bodies using aquatic plants or macrophytes are well established and are recognized as eco-friendly world over. Phytoremediation of heavy metals and other pollutants in aquatic environments can be achieved by using Ceratophyllum demersum L. - a well-known floating macrophyte. In vitro regenerated plants of C. demersum (7.5 g/L) were exposed to 24, 72, and 120 h to 0, 0.5, 1.0, 2.0, and 4.0 mg/L of cadmium (CdSO4·8H2O) in water. Results revealed significantly different relationship in terms of Cd in water, Cd uptake by plants, bioconcentration factor (BCF), and Cd removal (%) from water. The study showed that Cd uptake by plants and BCF values increased significantly with exposure time. The highest BCF value (3776.50) was recorded for plant samples exposed to 2 mg/L Cd for 72 h. Application of all Cd concentrations and various exposure duration yielded Cd removal (%) between the ranges of 93.8 and 98.7%. These results were predicted through artificial intelligence-based models, namely, random forest (RF), extreme gradient boosting (XGBoost), and multilayer perceptron (MLP). The tested models predicted the results accurately, and the attained results were further validated via three different performance metrics. The optimal regression coefficient (R2) for the models was recorded as 0.7970 (Cd water, mg/L), 0.9661 (Cd plants, mg/kg), 0.9797 bioconcentration factor (BCF), and 0.9996 (Cd removal, %), respectively. These achieved results suggest that in vitro regenerated C. demersum can be efficaciously used for phytoremediation of Cd-contaminated aquatic environments. Likewise, the proposed modeling of phytoremediation studies can further be employed more comprehensively in future studies aimed at data prediction and optimization.

Innovation in the Breeding of Common Bean Through a Combined Approach of in vitro Regeneration and Machine Learning Algorithms.

Common bean is considered a recalcitrant crop for in vitro regeneration and needs a repeatable and efficient in vitro regeneration protocol for its improvement through biotechnological approaches. In this study, the establishment of efficient and reproducible in vitro regeneration followed by predicting and optimizing through machine learning (ML) models, such as artificial neural network algorithms, was performed. Mature embryos of common bean were pretreated with 5, 10, and 20 mg/L benzylaminopurine (BAP) for 20 days followed by isolation of plumular apice for in vitro regeneration and cultured on a post-treatment medium containing 0.25, 0.50, 1.0, and 1.50 mg/L BAP for 8 weeks. Plumular apice explants pretreated with 20 mg/L BAP exerted a negative impact and resulted in minimum shoot regeneration frequency and shoot count, but produced longer shoots. All output variables (shoot regeneration frequency, shoot counts, and shoot length) increased significantly with the enhancement of BAP concentration in the post-treatment medium. Interaction of the pretreatment × post-treatment medium revealed the need for a specific combination for inducing a high shoot regeneration frequency. Higher shoot count and shoot length were achieved from the interaction of 5 mg/L BAP × 1.00 mg/L BAP followed by 10 mg/L BAP × 1.50 mg/L BAP and 20 mg/L BAP × 1.50 mg/L BAP. The evaluation of data through ML models revealed that R 2 values ranged from 0.32 to 0.58 (regeneration), 0.01 to 0.22 (shoot counts), and 0.18 to 0.48 (shoot length). On the other hand, the mean squared error values ranged from 0.0596 to 0.0965 for shoot regeneration, 0.0327 to 0.0412 for shoot count, and 0.0258 to 0.0404 for shoot length from all ML models. Among the utilized models, the multilayer perceptron model provided a better prediction and optimization for all output variables, compared to other models. The achieved results can be employed for the prediction and optimization of plant tissue culture protocols used for biotechnological approaches in a breeding program of common beans.