Relative biochemical and physiological docking of cucumber varieties for supporting innate immunity against Podosphaera xanthii.

Powdery mildew in cucumber is caused by the Podosphaera xanthii. No strategy for improving disease resistance can be successful in the absence of thorough insights into the physiological and biochemical responses of cucumber plants to powdery mildew. Therefore, a field experiment was executed to evaluate five commercial cucumber varieties (V1: Dynasty, V2: Long green, V3:Desi Kheera, V4:Thamin II, V5:Cucumber 363) for their inherent immunity to powdery mildew. Upon inoculating cucumber plants with Podosphaera xanthii, we noted differential responses among the varieties. Compared to other varieties, V1 and V2 showed higher values (P ≤ 0.05) for chlorophyll-a under control and pathogen-attacked plants respectively. The minimum value of anthocyanin content (-53.73%) was recorded in V3 as compared to other varieties post pathogen infection. All pathogen-infected cucumber varieties showed a considerable (P ≤ 0.05) loss in flavonoid content except V2. The maximum destruction for Phenolics under powdery mildew (179%) were recorded in V4, whereas V1 exhibited maximum phenolic content under control conditions. In pathogen-infected plants, the minimum AsA was recorded in V5 as compared to all other varieties. Pathogen invasion impacted significantly (P ≤ 0.05) the activity of superoxide dismutase (SOD). Besides, cucumber plants after pathogen inoculation resulted in a considerable (P ≤ 0.05) increase of peroxidase (POD) activity in V1 (5.02%), V2 (7.5%), and V3 (11%) in contrast to V4. Our results confirmed that cucumber varieties perform differently, which was brought on by distinct metabolic and physiological modifications that have an impact on growth and development. The changes in different attributes were correlated with cucumber resistance against powdery mildew. The results would help us fully harness the potential of these varieties to trigger disease management initiatives and defense responses.

Climate change reshaping plant-fungal interaction.

Plant diseases pose a severe threat to modern agriculture, necessitating effective and lasting control solutions. Environmental factors significantly shape plant ecology. Human-induced greenhouse gas emissions have led to global temperature rise, impacting various aspects, including carbon dioxide (CO2) concentration, temperature, ozone (O3), and ultraviolet-B, all of which influence plant diseases. Altered pathogen ranges can accelerate disease transmission. This review explores environmental effects on plant diseases, with climate change affecting fungal biogeography, disease incidence, and severity, as well as agricultural production. Moreover, we have discussed how climate change influences pathogen development, host-fungal interactions, the emergence of new races of fungi, and the dissemination of emerging fungal diseases across the globe. The discussion about environment-mediated impact on pattern-triggered immunity (PTI), effector-triggered immunity (ETI), and RNA interference (RNAi) is also part of this review. In conclusion, the review underscores the critical importance of understanding how climate change is reshaping plant-fungal interactions. It highlights the need for continuous research efforts to elucidate the mechanisms driving these changes and their ecological consequences. As the global climate continues to evolve, it is imperative to develop innovative strategies for mitigating the adverse effects of fungal pathogens on plant health and food security.

Silicon a key player to mitigate chromium toxicity in plants: Mechanisms and future prospective.

Chromium is a serious heavy metal (HM) and its concentration in plant-soil interface is soaring due to anthropogenic activities, unregulated disposals, and lack of efficient treatments. High concentration of Cr is toxic to ecosystems and human health. Cr stress also diminishes the plant performance by changing the plant's vegetative and reproductive development that ultimately affects sustainable crop production. Silicon (Si) is the second-most prevalent element in the crust of the planet, and has demonstrated a remarkable potential to minimize the HM toxicity. Amending soils with Si mitigates adverse effects of Cr by improving plant physiological, biochemical, and molecular functioning and ensuring better Cr immobilization, compartmentation, and co-precipitation. However, there is no comprehensive review on the role of Si to mitigate Cr toxicity in plants. Thus, in this present review; the discussion has been carried on; 1) the source of Cr, 2) underlying mechanisms of Cr uptake by plants, 3) how Si affects the plant functioning to reduce Cr toxicity, 4) how Si can cause immobilization, compartmentation, and co-precipitation 5) strategies to improve Si accumulation in plants to counter Cr toxicity. We also discussed the knowledge gaps and future research needs. The present review reports up-to-date knowledge about the role of Si to mitigate Cr toxicity and it will help to get better crop productivity in Cr-contaminated soils. The findings of the current review will educate the readers on Si functions in reducing Cr toxicity and will offer new ideas to develop Cr tolerance in plants through the use of Si.

Plant Growth-Promoting Rhizobacteria (PGPR) Assisted Bioremediation of Heavy Metal Toxicity.

Due to a variety of natural and anthropogenic processes, heavy metal toxicity of soil constitutes a substantial hazard to all living beings in the environment. The heavy metals alter the soil properties, which directly or indirectly influence the agriculture systems. Thus, plant growth-promoting rhizobacteria (PGPR)-assisted bioremediation is a promising, eco-friendly, and sustainable method for eradicating heavy metals. PGPR cleans up the heavy metal-contaminated environment using various approaches including efflux systems, siderophores and chelation, biotransformation, biosorption, bioaccumulation, precipitation, ACC deaminase activity, biodegradation, and biomineralization methods. These PGPRs have been found effective to bioremediate the heavy metal-contaminated soil through increased plant tolerance to metal stress, improved nutrient availability in soil, alteration of heavy metal pathways, and by producing some chemical compounds like siderophores and chelating ions. Many heavy metals are non-degradable; hence, another remediation approach with a broader scope of contamination removal is needed. This article also briefly emphasized the role of genetically modified PGPR strains which improve the soil's degradation rate of heavy metals. In this regard, genetic engineering, a molecular approach, could improve bioremediation efficiency and be helpful. Thus, the ability of PGPRs can aid in heavy metal bioremediation and promote a sustainable agricultural soil system.

Jasmonic acid boosts the salt tolerance of kidney beans (Phaseolus vulgaris L.) by upregulating its osmolytes and antioxidant mechanism.

As a lipid-derived compound, jasmonic acid (JA) regulates growth and defense against environmental stresses. An exogenous foliar JA application was investigated in our study (HA; 0.5 mM) on kidney bean plants (Phaseolus vulgaris L.) grown under different salinity stress concentrations (0, 75, and 150 mM NaCl). According to the results, salt concentrations were related to an increase in malondialdehyde (MDA) levels, whereas they declined the chlorophyll content index. In contrast, JA application decreased the level of MDA but increased the chlorophyll content index. Moreover, increasing salinity levels increased proline, phenolic compounds, flavonoids, free amino acid concentrations, and shikimic acid concentrations, as well as the activities of polyphenol oxidase (PPO), ascorbate peroxidase (APX), catalase (CAT), and peroxidase (POD). In addition, JA applications further increased their concentrations with increasing salinity stress levels. JA application increases salt-induced osmolytes and non-enzymatic antioxidants while increasing enzymatic antioxidant activity, suggesting kidney beans have a strong antioxidant mechanism, which can adapt to salinity stress. Our results showed that exogenous JA foliar applications could enhance the salt tolerance ability of kidney bean plants by upregulating their antioxidant mechanism and osmolytes.

Silicon Induces Heat and Salinity Tolerance in Wheat by Increasing Antioxidant Activities, Photosynthetic Activity, Nutrient Homeostasis, and Osmo-Protectant Synthesis.

Modern agriculture is facing the challenges of salinity and heat stresses, which pose a serious threat to crop productivity and global food security. Thus, it is necessary to develop the appropriate measures to minimize the impacts of these serious stresses on field crops. Silicon (Si) is the second most abundant element on earth and has been recognized as an important substance to mitigate the adverse effects of abiotic stresses. Thus, the present study determined the role of Si in mitigating adverse impacts of salinity stress (SS) and heat stress (HS) on wheat crop. This study examined response of different wheat genotypes, namely Akbar-2019, Subhani-2021, and Faisalabad-2008, under different treatments: control, SS (8 dSm-1), HS, SS + HS, control + Si, SS + Si, HS+ Si, and SS + HS+ Si. This study's findings reveal that HS and SS caused a significant decrease in the growth and yield of wheat by increasing electrolyte leakage (EL), malondialdehyde (MDA), and hydrogen peroxide (H2O2) production; sodium (Na+) and chloride (Cl-) accumulation; and decreasing relative water content (RWC), chlorophyll and carotenoid content, total soluble proteins (TSP), and free amino acids (FAA), as well as nutrient uptake (potassium, K; calcium, Ca; and magnesium, Mg). However, Si application offsets the negative effects of both salinity and HS and improved the growth and yield of wheat by increasing chlorophyll and carotenoid contents, RWC, antioxidant activity, TSP, FAA accumulation, and nutrient uptake (Ca, K, and Mg); decreasing EL, electrolyte leakage, MDA, and H2O2; and restricting the uptake of Na+ and Cl-. Thus, the application of Si could be an important approach to improve wheat growth and yield under normal and combined saline and HS conditions by improving plant physiological functioning, antioxidant activities, nutrient homeostasis, and osmolyte accumulation.

Organic Amendments Improved the Productivity and Bio-Fortification of Fine Rice by Improving Physiological Responses and Nutrient Homeostasis under Salinity Stress.

Salinity stress (SS) is major abiotic stress that is seriously limiting crop production across the globe. The application of organic amendments (OA) mitigate the effects of salinity and improves soil health and crop production on a sustainable basis. However, limited studies are conducted to determine the impact of farmyard manure (FYM) and press mud (PM) on the performance of rice crop. Therefore, we performed this study to determine the impacts of FYM and PM on the growth, physiological and biochemical attributes, yield, and grain bio-fortification of rice crop under SS. The experiment was comprised of SS levels; control, 6 and 12 dS m-1 SS and OA; control, FYM: 5%, press mud 5% and combination of FYM (5%) + PM (5%). Soil salinity imposed deleterious impacts on the growth, yield, and grain quality of rice, however, OA appreciably offset the deleterious impacts of SS and improved the growth, yield, and grain bio-fortification of rice crop. The combined application of FYM + PM improved the growth and yield of rice through an increase in chlorophyll contents, leaf water contents, anti-oxidant activities (ascorbate peroxidise: APX; catalase: CAT, peroxidise: POD and ascorbic acid: AsA), K+ accumulation and decrease in Na+/K+ ratio, electrolyte leakage, malondialdehyde (MDA), hydrogen peroxide (H2O2), Na+ accumulation. Moreover, the combined application of FYM + PM significantly improved the grain protein (5.84% and 12.90%), grain iron (40.95% and 42.37%), and grain zinc contents (36.81% and 50.93%) at 6 and 12 dS m-1 SS. Therefore, this study suggested that the application of FYM and PM augmented the growth, yield, physiology, biochemistry, and grain bio-fortification of rice and proved to be a good practice for better rice production in salt-affected soils.

Combining Genome-Wide Gene Expression Analysis (RNA-seq) and a Gene Editing Platform (CRISPR-Cas9) to Uncover the Selectively Pro-oxidant Activity of Aurone Compounds Against Candida albicans.

Candida albicans is the major fungal cause of healthcare-associated bloodstream infections worldwide with a 40% mortality rate. The scarcity of antifungal treatments due to the eukaryotic origin of fungal cells has challenged the development of selectively antifungal drugs. In an attempt to identify novel antifungal agents, aurones SH1009 and SH9051, as synthetically bioactive compounds, have been recently documented as anti-Candida agents. Since the molecular mechanisms behind the inhibitory activities of these aurones in C. albicans are unclear, this study aimed to determine the comprehensive cellular processes affected by these aurones and their molecular targets. Genome-wide transcriptional analysis of SH1009- and SH9051-treated C. albicans revealed uniquely repressed expression in different metabolic pathways, particularly trehalose and sulfur amino acid metabolic processes for SH1009 and SH9051, respectively. In contrast, the most commonly enriched process for both aurones was the up-regulation of RNA processing and ribosomal cleavages as an indicator of high oxidative stress, suggesting that a common aspect in the chemical structure of both aurones led to pro-oxidative properties. Additionally, uniquely induced responses (iron ion homeostasis for SH1009 and arginine biosynthesis for SH9051) garnered attention on key roles for the aurone functional groups. Deletion of the transcription factor for the trehalose biosynthesis pathway, Tye7p, resulted in an SH1009-resistant mutant, which also exhibited low trehalose content, validating the primary molecular target of SH1009. Aurone SH9051 uniquely simulated an exogenous supply of methionine or cysteine, leading to sulfur amino acid catabolism as evidenced by quantifying an overproduction of sulfite. Phenyl aurone, the common structure of aurones, contributed proportionally in the pro-oxidative activity through ferric ion reduction effects leading to high ROS levels. Our results determined selective and novel molecular mechanisms for aurone SH1009 and also elucidated the diverse cellular effects of different aurones based on functional groups.

Chemogenomic profiling to understand the antifungal action of a bioactive aurone compound.

Every year, more than 250,000 invasive candidiasis infections are reported with 50,000 deaths worldwide. The limited number of antifungal agents necessitates the need for alternative antifungals with potential novel targets. The 2-benzylidenebenzofuran-3-(2H)-ones have become an attractive scaffold for antifungal drug design. This study aimed to determine the antifungal activity of a synthetic aurone compound and characterize its mode of action. Using the broth microdilution method, aurone SH1009 exhibited inhibition against C. albicans, including resistant isolates, as well as C. glabrata, and C. tropicalis with IC50 values of 4-29 µM. Cytotoxicity assays using human THP-1, HepG2, and A549 human cell lines showed selective toxicity toward fungal cells. The mode of action for SH1009 was characterized using chemical-genetic interaction via haploinsufficiency (HIP) and homozygous (HOP) profiling of a uniquely barcoded Saccharomyces cerevisiae mutant collection. Approximately 5300 mutants were competitively treated with SH1009 followed by DNA extraction, amplification of unique barcodes, and quantification of each mutant using multiplexed next-generation sequencing. Barcode post-sequencing analysis revealed 238 sensitive and resistant mutants that significantly (FDR P values ≤ 0.05) responded to aurone SH1009. The enrichment analysis of KEGG pathways and gene ontology demonstrated the cell cycle pathway as the most significantly enriched pathway along with DNA replication, cell division, actin cytoskeleton organization, and endocytosis. Phenotypic studies of these significantly enriched responses were validated in C. albicans. Flow cytometric analysis of SH1009-treated C. albicans revealed a significant accumulation of cells in G1 phase, indicating cell cycle arrest. Fluorescence microscopy detected abnormally interrupted actin dynamics, resulting in enlarged, unbudded cells. RT-qPCR confirmed the effects of SH1009 in differentially expressed cell cycle, actin polymerization, and signal transduction genes. These findings indicate the target of SH1009 as a cell cycle-dependent organization of the actin cytoskeleton, suggesting a novel mode of action of the aurone compound as an antifungal inhibitor.