Field-based molecular detection of Batrachochytrium dendrobatidis in critically endangered Atelopus toads and aquatic habitats in Ecuador.

Batrachochytrium dendrobatidis (Bd) is a lethal fungal species that parasitizes vertebrates and is associated with the worldwide decline of amphibian populations. The development of sensitive, rapid detection methods, particularly DNA-based techniques, is critical for effective management strategies. This study evaluates the efficacy of DNA extraction and a portable PCR device in a mountable field laboratory setup for detecting Bd near the habitats of three critically endangered Atelopus toad species in Ecuador. We collected skin swabs from Atelopus balios, A. nanay, and A. bomolochos, and environmental DNA (eDNA) samples from streams in Andean and coastal regions of Ecuador. For eDNA, a comparison was made with duplicates of the samples that were processed in the field and in a standard university laboratory. Our findings revealed Bd detection in eDNA and swabs from 6 of 12 water samples and 10 of 12 amphibian swab samples. The eDNA results obtained in the field laboratory were concordant with those obtained under campus laboratory conditions. These findings highlight the potential of field DNA-based monitoring techniques for detecting Bd in amphibian populations and their aquatic habitats, particularly in remote areas. Furthermore, this research aligns with the National Action Plan for the Conservation of Ecuadorian Amphibians and contributes to the global effort to control this invasive and deadly fungus.

Impact of urbanization trends on production of key staple crops.

Urbanization has appropriated millions of hectares of cropland, and this trend will persist as cities continue to expand. We estimate the impact of this conversion as the amount of land needed elsewhere to give the same yield potential as determined by differences in climate and soil properties. Robust spatial upscaling techniques, well-validated crop simulation models, and soil, climate, and cropping system databases are employed with a focus on populous countries with high rates of land conversion. We find that converted cropland is 30-40% more productive than new cropland, which means that projection of food production potential must account for expected cropland loss to urbanization. Policies that protect existing farmland from urbanization would help relieve pressure on expansion of agriculture into natural ecosystems.

Spatial frameworks for robust estimation of yield gaps.

Food security interventions and policies need reliable estimates of crop production and the scope to enhance production on existing cropland. Here we assess the performance of two widely used 'top-down' gridded frameworks (Global Agro-ecological Zones and Agricultural Model Intercomparison and Improvement Project) versus an alternative 'bottom-up' approach (Global Yield Gap Atlas). The Global Yield Gap Atlas estimates extra production potential locally for a number of sites representing major breadbaskets and then upscales the results to larger spatial scales. We find that estimates from top-down frameworks are alarmingly unlikely, with estimated potential production being lower than current farm production at some locations. The consequences of using these coarse estimates to predict food security are illustrated by an example for sub-Saharan Africa, where using different approaches would lead to different prognoses about future cereal self-sufficiency. Our study shows that foresight about food security and associated agriculture research priority setting based on yield potential and yield gaps derived from top-down approaches are subject to a high degree of uncertainty and would benefit from incorporating estimates from bottom-up approaches.

Electrochemical study of hydroxychloroquine and its determination in plaquenil by differential pulse voltammetry.

Hydroxychloroquine (HCQ) is a halogenated aminoquinoline that presents wide biological activity, often being used as an antimalarial drug. The electrochemical reduction of HCQ was investigated by cyclic voltammetry and chronoamperometry using glassy carbon electrodes. By cyclic voltammetry, in acid medium, only the cathodic peak was observed. The electrochemical behavior of this peak is dependent on pH and the electrodic process occurs through an E(r)C(i) mechanism. The electron number (1e) consumed in the reduction of HCQ was obtained by chronoamperometry. A method for the electrochemical determination of HCQ in pharmaceutical tablets was developed using differential pulse voltammetry. The detection limit reached was 11.2 microg ml(-1) of HCQ with a relative standard deviation of 0.46%. A spectrophotometric study of HCQ has been also carried out utilizing a band at 343 nm. The obtained detection limit and the relative standard deviation were 0.1 microg ml(-1) and 0.36%, respectively. The electrochemical methods are sufficiently accurate and precise to be applied for HCQ determination, in laboratorial routine, which can be used to determine the drug at low level.