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Visual perception is formed over time through the formation process and visual pathway. Exercise improves visual perception, but it is unclear whether exercise modulates nonspecifically or specifically the formation process and pathway of visual perception. Healthy young men performed the visual detection task in a backward masking paradigm before and during cycling exercise at a mild intensity or rest (control). The task presented gratings of a circular patch (target) and annulus (mask) arranged concentrically as a visual stimulus and asked if the presence and striped pattern (feature) of the target were detected. The relationship between the orientations of the gratings of the target and the mask included iso-orientation and orthogonal orientation to investigate the orientation selectivity of the masking effect. The masking effect was evaluated by perceptual suppressive index (PSI). Exercise improved feature detection (∆PSI; Exercise: -20.6%, Control: 1.7%) but not presence detection (∆PSI; Exercise: 8.9%, Control: 29.6%) compared to the control condition, and the improving effect resulted from the attenuation of the non-orientation-selective (∆PSI; Exercise: -29.0%, Control: 16.8%) but not orientation-selective masking effect (∆PSI; Exercise: -3.1%, Control: 11.7%). These results suggest that exercise affects the formation process of the perceptual feature of the target stimulus by suppressively modulating the neural networks responsible for the non-orientation-selective surround interaction in the subcortical visual pathways, whose effects are inherited by the cortical visual pathways necessary for perceptual image formation. In conclusion, our findings suggest that acute exercise improves visual perception transiently through the modulation of a specific formation process of visual processing.
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Mascaramento Perceptivo , Córtex Visual , Masculino , Humanos , Percepção Visual , Exercício Físico , Estimulação Luminosa/métodosRESUMO
OBJECTIVES: Identify predictors of residual carcinoma or carcinoma-in-situ (CIS) at hysterectomy following cervical conizations with CIS and positive margins or endocervical curettage (ECC) or microinvasive cervical cancer. METHODS: Patients with cervical conization with CIS and positive margins, ECC or microinvasive carcinoma who underwent hysterectomy within 6 months of conization were identified. Conization and hysterectomy specimens were re-reviewed to assess volume of disease, ECC and margin status and residual carcinoma. Standard statistical tests were used. RESULTS: 83 patients were included. 34 (41%) had residual carcinoma in the hysterectomy specimen: 23 CIS, 9 microinvasive and 2 invasive disease. In patients with squamous histology predictors of residual disease included a positive ECC (p=0.04), combined endocervical margin and ECC (69% if both positive, 38% either positive, 11% if both negative, p=0.01) and volume of disease ≥ 50% (p=0.01). In patients with glandular histology no factor predicted residual disease. Type of conization, >2 involved quadrants, and the presence of microinvasion in the conization specimen did not predict residual disease. No patient with squamous histology had >Stage IA1 disease at hysterectomy, whereas 2 (2.4%) with adenocarcinoma had >Stage IA1 disease at hysterectomy. CONCLUSIONS: Residual carcinoma or CIS is present in nearly half of hysterectomies after conization with CIS and positive ECC, margins or microinvasion. Patients with squamous histology may not require repeat conization prior to definitive therapy. No factors predict residual disease with adenocarcinoma. In women with AIS with negative margins and ECC and no microinvasion, it appears reasonable to proceed with simple hysterectomy.
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Carcinoma in Situ/cirurgia , Colo do Útero/cirurgia , Conização , Histerectomia , Neoplasias do Colo do Útero/cirurgia , Adulto , Idoso , Carcinoma in Situ/patologia , Feminino , Humanos , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Neoplasias do Colo do Útero/patologiaRESUMO
Visuomotor performance acting on a moving target is fundamentally based on visual motion discriminability, and its neural basis is presumed to be human MT (hMT+), a motion vision center of the dorsal visual pathway. In this study, we investigated whether and how the accuracy and speed of motion discrimination are affected by applying transcranial static magnetic field stimulation (tSMS) to hMT+, which reduces cortical excitability. Sixteen participants performed a motion direction discrimination (MDD) task using a random dot kinematogram before (Pre-test) and during (During-test) application of the tSMS over left hMT+. The correct rate of the MDD task was significantly lower in the During-test compared to the Pre-test, an effect not seen with the sham condition. The inhibition effects were observed only for the right visual field corresponding to hMT+ in the stimulated hemisphere. On the other hand, no modulatory effect of tSMS was observed in the reaction time. We, therefore, demonstrated the inhibitory effect of tSMS on the left hMT+ impairs the accuracy but not the speed of motion information processing in the contralateral visual field.
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Percepção de Movimento , Estimulação Magnética Transcraniana , Humanos , Percepção de Movimento/fisiologia , Movimento (Física) , Tempo de Reação , Campos Magnéticos , Estimulação LuminosaRESUMO
Introduction: Table tennis players perform visually guided visuomotor responses countlessly. The exposure of the visual system to frequent and long-term motion stimulation has been known to improve perceptual motion detection and discrimination abilities as a learning effect specific to that stimulus, so may also improve visuo-oculomotor performance. We hypothesized and verified that table tennis players have good spatial accuracy of saccades to moving targets. Methods: University table tennis players (TT group) and control participants with no striking-sports experience (Control group) wore a virtual reality headset and performed two ball-tracking tasks to track moving and stationary targets in virtual reality. The ball moved from a predetermined position on the opponent's court toward the participant's court. A total of 54 conditions were examined for the moving targets in combinations of three ball trajectories (familiar parabolic, unfamiliar descent, and unfamiliar horizontal), three courses (left, right, and center), and six speeds. Results and discussion: All participants primarily used catch-up saccades to track the moving ball. The TT group had lower mean and inter-trial variability in saccade endpoint error compared to the Control group, showing higher spatial accuracy and precision, respectively. It suggests their improvement of the ability to analyze the direction and speed of the ball's movement and predict its trajectory and future destination. The superiority of the spatial accuracy in the TT group was seen in both the right and the left courses for all trajectories but that of precision was for familiar parabolic only. The trajectory dependence of improved saccade precision in the TT group implies the possibility that the motion vision system is trained by the visual stimuli frequently encountered in table tennis. There was no difference between the two groups in the onset time or spatial accuracy of saccades for stationary targets appearing at various positions on the ping-pong table. Conclusion: Table tennis players can obtain high performance (spatial accuracy and precision) of saccades to track moving targets as a result of motion vision ability improved through a vast amount of visual and visuo-ocular experience in their play.
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Background: Precise postural control helps prevent anterior cruciate ligament injury. However, it is unknown whether the anticipated postural stability can be improved during a physically uncertain and cognitively demanding task. Hypothesis: Anticipated postural stability will improve through unanticipated single-leg landing with a rapid foot placement target tracking. Study Design: Controlled laboratory study. Methods: A total of 22 healthy female university-level athletes performed a novel dual-task paradigm: an unanticipated single-leg landing with foot placement target tracking. In the normal condition (60 trials), the participants jumped from a 20 cm-high box onto the landing target with their dominant leg as softly as possible. In the subsequent perturbation condition (PC) (60 trials), the initially assigned landing target was abruptly switched randomly, requiring participants to modify their preplanned foot placement position to the newly assigned position. The center-of-pressure trajectory length within the first 100 ms after foot impact (CoP100) was calculated as a measure of anticipated postural stability for each trial. In addition, the peak vertical ground-reaction force (FzPeak) was quantified to assess landing load, and the degree of postural adaptation during PC was quantified by fitting an exponential function to trial-by-trial changes in CoP100. Participants were divided into 2 groups according to increase or decrease in CoP100, and results were compared between the groups. Results: The direction and magnitude of postural sway alterations of the 22 participants showed a spectrum-like variation during the repeated trials. Twelve participants (sway-decreased group) exhibited a gradual reduction in postural sway (CoP100) during the PC, while the remaining 10 participants (sway-increased group) showed a gradual increase in CoP100. The FzPeak during the PC was significantly less in the sway-decreased group compared with the sway-increased group (P < .05). Conclusion: Variation in the direction and magnitude of postural sway alteration among participants suggested that there was individual variation in an athlete's adaptive ability of the anticipated postural stability. Clinical Relevance: The novel dual-task paradigm described in this study may be useful for rating individual injury risk based on an athlete's postural adaptation ability and may aid in targeted prevention strategies.
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OBJECTIVE: To identify clinical and pathologic predictors of response to progestin treatment in premenopausal women with complex atypical hyperplasia (CAH) and Grade 1 endometrial adenocarcinoma (Grade 1 EA). METHODS: Forty premenopausal patients with Grade 1 EA or CAH who underwent progestin therapy for a minimum of 8 weeks were retrospectively identified. Patient characteristics and histopathologic features of pretreatment and first follow-up endometrial specimens were evaluated as predictors of resolution, defined as absence of hyperplasia or carcinoma. RESULTS: Kaplan-Meier analysis indicated 63% resolution at 18 months of follow-up. Multivariate classification analysis showed that resolution rates were higher in individuals with a low pre-treatment qualitative abnormal architecture score and a BMI <35 (Standardized Resolution Ratio (SRR)=1.48, p=0.03). The diagnosis of benign endometrium or simple hyperplasia on the first follow-up specimen was highly predictive of resolution (SRR=2.25, p=0.002). Resolution rates were lower among subjects with a high pre-treatment qualitative abnormal architecture score (SRR=0.37, p<0.03) and lowest in subjects whose first follow-up specimen showed persistent complexity, atypia, or carcinoma with adjacent stromal decidualization (SRR=0.24, p=0.002). CONCLUSIONS: Clinical and pathologic parameters can predict response to progestin therapy in premenopausal women with CAH and Grade 1 EA. A low likelihood of resolution is predicted by an unfavorable pre-treatment architectural score and lack of pathological response in the first specimen, despite adjacent stromal decidualization.
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Adenocarcinoma/tratamento farmacológico , Adenocarcinoma/patologia , Hiperplasia Endometrial/tratamento farmacológico , Hiperplasia Endometrial/patologia , Neoplasias do Endométrio/tratamento farmacológico , Neoplasias do Endométrio/patologia , Progestinas/uso terapêutico , Adulto , Estudos de Coortes , Feminino , Humanos , Análise Multivariada , Lesões Pré-Cancerosas/tratamento farmacológico , Lesões Pré-Cancerosas/patologia , Pré-Menopausa , Estudos RetrospectivosRESUMO
In ball sports such as table tennis, in which a ball moving at high speed is hit, an athlete's brain needs to process the motion information of the ball, predict the arrival point, and form a motor command to direct the racket there. Therefore, day-to-day fluctuations in visuomotor performance may be ascribed to fluctuations in visual motion discriminability, but it is not clear how the two are related. To examine this point, university table tennis players performed a motion direction discrimination (MDD) task and continuous visuomotor (CVM) task over 10 days as an estimation of visual motion discriminability and visuomotor performance, respectively. In the MDD task, using a joystick, participants distinguished the direction of a global coherent motion of target dots moving in the same direction on a PC monitor from innumerable dots moving in random directions. In the CVM task, participants hit sequential targets moving fast from right to left on the PC monitor by operating the cursor on the left side of the monitor up and down using the prehensile force of their thumb and index finger. The scores in the MDD and CVM tasks fluctuated day by day and showed a significant and moderate correlation between the MDD task score for the visual field in which the participants captured the target in the CVM task and the CVM task score. This correlation was confirmed even with the target moving from left to right. The fluctuations in the onset latency and the endpoint position of the cursor movement approaching the target were correlated with those of the visual motion discriminability, suggesting the contribution of motion vision to the speed and accuracy of the visuomotor performance. Moreover, these relationships were prominent in veteran players. For table tennis athletes, especially experienced players, fluctuations in the visual motion discrimination performance in a visual field specific for capturing a ball may be responsible for the fluctuations in continuous visuomotor (striking) performance.
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This study investigated the changes in physical inactivity of university students during the COVID-19 pandemic, with reference to their academic calendar. We used the daily step counts recorded by a smartphone application (iPhone Health App) from April 2020 to January 2021 (287 days) for 603 participants. The data for 287 days were divided into five periods based on their academic calendar. The median value of daily step counts across each period was calculated. A k-means clustering analysis was performed to classify the 603 participants into subgroups to demonstrate the variability in the physical inactivity responses. The median daily step counts, with a 7-day moving average, dramatically decreased from 5000 to 2000 steps/day in early April. It remained at a lower level (less than 2000 steps/day) during the first semester, then increased to more than 5000 steps/day at the start of summer vacation. The clustering analysis demonstrated the variability in physical inactivity responses. The inactive students did not recover daily step counts throughout the year. Consequently, promoting physical activity is recommended for inactive university students over the course of the whole semester.
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COVID-19 , Aplicativos Móveis , COVID-19/epidemiologia , Humanos , Pandemias , SARS-CoV-2 , Comportamento Sedentário , Smartphone , Estudantes , UniversidadesRESUMO
In a table tennis rally, players perform interceptive actions on a moving ball continuously in a short time, such that the acquisition process of visual information is an important determinant of the performance of the action. However, because it is technically hard to measure gaze movement in a real game, little is known about how gaze behavior is conducted during the continuous visuomotor actions and contributes to the performance. To examine these points, we constructed a novel psychophysical experiment model enabling a continuous visuomotor task without spatial movement of any body parts, including the arm and head, and recorded the movement of the gaze and effector simultaneously at high spatiotemporal resolution. In the task, Gabor patches (target) moved one after another at a constant speed from right to left at random vertical positions on an LC display. Participants hit the target with a cursor moving vertically on the left side of the display by controlling their prehensile force on a force sensor. Participants hit the target with the cursor using a rapid-approaching movement (rapid cursor approach, RCA). Their gaze also showed rapid saccadic approaching movement (saccadic eye approach, SEA), reaching the predicted arrival point of the target earlier than the cursor. The RCA reached in or near the Hit zone in the successful (Hit) trial, but ended up away from it in the unsuccessful (Miss) trial, suggesting the spatial accuracy of the RCA determines the task's success. The SEA in the Hit trial ended nearer the target than the Miss trial. The spatial accuracy of the RCA diminished when the target disappeared 100 ms just after the end of the SEA, suggesting that visual information acquired after the saccade acted as feedback information to correct the cursor movement online for the cursor to reach the target. There was a target speed condition that the target disappearance did not compromise RCA's spatial accuracy, implying the possible RCA correction based on the post-saccadic gaze location information. These experiments clarified that gaze behavior conducted during fast continuous visuomotor actions enables online correction of the ongoing interceptive movement of an effector, improving visuomotor performance.
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Acute aerobic exercise increases the brain cortical activity in alpha frequency. Eye closure also increases alpha activity. However, whether the two have an additive or a synergistic effect on alpha activity has never been explored. This study observed electroencephalography (EEG) from fifteen participants seated on the cycle ergometer before, during, and after a cycling exercise with the eyes open and with them closed. Exercise intensity was set to a target heart rate (120-130 bpm), corresponding to light-to-moderate intensity exercise. Each epoch was 6 min and the last 4 min (eyes closed in the first 2 min and eyes open in the second 2 min) were analyzed. The EEG power spectrum densities were calculated for alpha frequency band activity (8-13 Hz). At rest, alpha activity was significantly greater with the eyes closed than open. Exercise significantly increased alpha activity in both eye conditions. More importantly, in the occipital site, the alpha-increasing effect of their combination was significantly greater than the sum of the effect of each, showing a synergistic effect. We concluded that acute light-to-moderate intensity exercise with the eyes closed has a synergistic effect on alpha activity.
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Encéfalo/fisiologia , Exercício Físico/fisiologia , Fenômenos Fisiológicos Oculares , Eletroencefalografia , Ergometria , Feminino , Frequência Cardíaca , Humanos , Masculino , Adulto JovemRESUMO
PURPOSE: Smoking is an epidemiologic risk factor for cervical cancer. The fragile histidine triad (FHIT) gene is a tumor suppressor gene that is altered in 80% of tobacco-associated lung cancers. We hypothesized that reduced FHIT protein expression, homozygous deletions (HD) or hemizygous deletions (HemiD) and microsatellite alterations (MA) at the FHIT/FRA3B locus occur more commonly in cervical cancers of smokers than nonsmokers. EXPERIMENTAL DESIGN: Archival tissues of 58 patients with stage IA1 to IB2 squamous cell carcinoma of the cervix were identified. FHIT protein expression was studied with immunohistochemistry. Laser capture microdissection was used to isolate tumor and normal DNA. HD/HemiD of FHIT exons 4 and 5 were analyzed by monoplex real-time PCR. MA at FHIT/FRA3B were studied with multiplex nested PCR with three fluorescently labeled microsatellite markers (D3S1300, D3S1312, and D3S1480). RESULTS: Eighteen of 26 tumors from smokers (69%) and 13 of 32 nonsmokers (41%; P < 0.05) showed loss of FHIT protein expression. Thirty-seven stage IB tumors yielded sufficient DNA for analyses. HD or HemiD of both exons tested occurred in 8 of 17 smokers (47%) and 2 of 20 nonsmokers (10%; P < 0.05). MA at more than two sites were found in 11 of 17 tumors of smokers (65%) and 6 of 20 nonsmokers (30%; P < 0.05). Mean composite genomic FHIT alteration scores were significantly higher for tumors of smokers versus nonsmokers (0.67 versus 0.40; P < 0.02). CONCLUSION: Loss of FHIT expression, HD, HemiD, and MA at the FHIT/FRA3B locus occur significantly more commonly in cervical cancers of smokers. These findings suggest that the tumor suppressor gene FHIT may represent a molecular target in cigarette smoking-associated cervical carcinogenesis.
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Hidrolases Anidrido Ácido/genética , Carcinoma de Células Escamosas/genética , Proteínas de Neoplasias/genética , Fumar/efeitos adversos , Neoplasias do Colo do Útero/genética , Hidrolases Anidrido Ácido/análise , Adulto , Carcinoma de Células Escamosas/etiologia , Carcinoma de Células Escamosas/metabolismo , Feminino , Deleção de Genes , Homozigoto , Humanos , Imuno-Histoquímica , Repetições de Microssatélites , Pessoa de Meia-Idade , Proteínas de Neoplasias/análise , Estadiamento de Neoplasias , Reação em Cadeia da Polimerase/métodos , Neoplasias do Colo do Útero/etiologia , Neoplasias do Colo do Útero/metabolismoRESUMO
Progesterone plays an essential role in breast development and cancer formation. The local metabolism of progesterone may limit its interactions with the progesterone receptor (PR) and thereby act as a prereceptor regulator. Selective loss of AKR1C1, which encodes a 20alpha-hydroxysteroid dehydrogenase [20alpha-HSD (EC 1.1.1.149)], and AKR1C2, which encodes a 3alpha-hydroxysteroid dehydrogenase [3alpha-HSD (EC 1.1.1.52)], was found in 24 paired breast cancer samples as compared with paired normal tissues from the same individuals. In contrast, AKR1C3, which shares 84% sequence identity, and 5alpha-reductase type I (SRD5A1) were minimally affected. Breast cancer cell lines T-47D and MCF-7 also expressed reduced AKR1C1, whereas the breast epithelial cell line MCF-10A expressed AKR1C1 at levels comparable with those of normal breast tissues. Immunohistochemical staining confirmed loss of AKR1C1 expression in breast tumors. AKR1C3 and AKR1C1 were localized on the same myoepithelial and luminal epithelial cell layers. Suppression of ARK1C1 and AKR1C2 by selective small interfering RNAs inhibited production of 20alpha-dihydroprogesterone and was associated with increased progesterone in MCF-10A cells. Suppression of AKR1C1 alone or with AKR1C2 in T-47D cells led to decreased growth in the presence of progesterone. Overexpression of AKR1C1 and, to a lesser extent, AKR1C2 (but not AKR1C3) decreased progesterone-dependent PR activation of a mouse mammary tumor virus promoter in both prostate (PC-3) and breast (T-47D) cancer cell lines. We speculate that loss of AKR1C1 and AKR1C2 in breast cancer results in decreased progesterone catabolism, which, in combination with increased PR expression, may augment progesterone signaling by its nuclear receptors.
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20-Hidroxiesteroide Desidrogenases/deficiência , Neoplasias da Mama/enzimologia , Hidroxiesteroide Desidrogenases/deficiência , Progesterona/fisiologia , 20-Hidroxiesteroide Desidrogenases/antagonistas & inibidores , 20-Hidroxiesteroide Desidrogenases/biossíntese , 20-Hidroxiesteroide Desidrogenases/genética , Sequência de Aminoácidos , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Humanos , Hidroxiesteroide Desidrogenases/antagonistas & inibidores , Hidroxiesteroide Desidrogenases/biossíntese , Hidroxiesteroide Desidrogenases/genética , Imuno-Histoquímica , Vírus do Tumor Mamário do Camundongo/genética , Dados de Sequência Molecular , Progesterona/antagonistas & inibidores , Progesterona/metabolismo , Regiões Promotoras Genéticas , RNA Interferente Pequeno/genética , Receptores de Progesterona/genética , Receptores de Progesterona/metabolismo , Receptores de Progesterona/fisiologia , Transdução de Sinais/fisiologia , Ativação Transcricional , TransfecçãoRESUMO
The brain dynamically changes its input-output relationship depending on the behavioral state and context in order to optimize information processing. At the molecular level, cholinergic/monoaminergic transmitters have been extensively studied as key players for the state/context-dependent modulation of brain function. In this paper, we review how cortical visual information processing in the primary visual cortex (V1) of macaque monkey, which has a highly differentiated laminar structure, is optimized by serotonergic and cholinergic systems by examining anatomical and in vivo electrophysiological aspects to highlight their similarities and distinctions. We show that these two systems have a similar layer bias for axonal fiber innervation and receptor distribution. The common target sites are the geniculorecipient layers and geniculocortical fibers, where the appropriate gain control is established through a geniculocortical signal transformation. Both systems exert activity-dependent response gain control across layers, but in a manner consistent with the receptor subtype. The serotonergic receptors 5-HT1B and 5HT2A modulate the contrast-response curve in a manner consistent with bi-directional response gain control, where the sign (facilitation/suppression) is switched according to the firing rate and is complementary to the other. On the other hand, cholinergic nicotinic/muscarinic receptors exert mono-directional response gain control without a sign reversal. Nicotinic receptors increase the response magnitude in a multiplicative manner, while muscarinic receptors exert both suppressive and facilitative effects. We discuss the implications of the two neuromodulator systems in hierarchical visual signal processing in V1 on the basis of the developed laminar structure.
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Haplorrinos/fisiologia , Receptores Colinérgicos/metabolismo , Neurônios Serotoninérgicos/metabolismo , Percepção Visual/fisiologia , Animais , Receptores Muscarínicos/metabolismo , Receptores Nicotínicos/metabolismo , Córtex Visual/fisiologiaRESUMO
Atypical squamous lesion (ASL), a histologic diagnosis of unclear significance in the uterine cervix, can be divided into neoplastic and nonneoplastic groups. We aimed to determine the morphologic characteristics of these 2 groups. Histologic and immunohistochemical features were evaluated on the original biopsy specimen from 37 ASL cases, and the results were compared between neoplastic (19 cases) and nonneoplastic (18 cases) groups, which were determined based on the follow-up histopathologic findings. Mitosis, vertical nuclear growth pattern, no perinuclear halo, indistinct cytoplasmic border, primitive cells in the upper third of the squamous layer, p16+ cells in the upper two thirds of the squamous layer, and Ki-67+ cells in the upper two thirds of the squamous layer were significant indicators for neoplastic ASLs. Of the 19 neoplastic ASLs, 16 (84%) had 5 or more of these 7 indicators. The majority (16/18 [89%]) of the nonneoplastic ASLs had 2 or fewer indicators. Determination of the histologic and immunohistochemical characteristics is useful for distinguishing neoplastic and nonneoplastic ASLs.
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Carcinoma de Células Escamosas/diagnóstico , Displasia do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/diagnóstico , Adulto , Idoso , Biomarcadores Tumorais/análise , Carcinoma de Células Escamosas/química , Carcinoma de Células Escamosas/classificação , Núcleo Celular/patologia , Inibidor p16 de Quinase Dependente de Ciclina/análise , Citoplasma/patologia , Feminino , Seguimentos , Humanos , Antígeno Ki-67/análise , Pessoa de Meia-Idade , Mitose , Displasia do Colo do Útero/química , Displasia do Colo do Útero/classificação , Neoplasias do Colo do Útero/química , Neoplasias do Colo do Útero/classificaçãoRESUMO
BACKGROUND: Approximately 24% of American adults have tattoos. Studies of humans and mice demonstrate that tattoo pigment migrates to lymph nodes and can cause lymphadenopathy. CASE: A 32-year-old woman presented with a 6-cm vulvar mass and extensive bilateral inguinofemoral lymphadenopathy. Bilateral small tattoos were noted in the groins. Vulvar biopsy confirmed squamous cell carcinoma, and fine needle aspiration of the lymph nodes showed no evidence of malignancy. The patient underwent a radical hemivulvectomy and bilateral inguinofemoral lymphadenectomy. Both inguinal and femoral nodes were enlarged because of extracellular tattoo pigment and reactive follicular hyperplasia without any evidence of metastasis. CONCLUSION: This case emphasizes the need to consider tattoo pigment as a cause of lymphadenopathy in any patient with a regional tattoo.
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Carcinoma de Células Escamosas/patologia , Corantes/efeitos adversos , Doenças Linfáticas/induzido quimicamente , Tatuagem , Neoplasias Vulvares/patologia , Adulto , Biópsia por Agulha Fina , Carcinoma de Células Escamosas/induzido quimicamente , Diagnóstico Diferencial , Feminino , Virilha/patologia , Humanos , Linfonodos/patologia , Doenças Linfáticas/cirurgia , Neoplasias Vulvares/induzido quimicamenteAssuntos
Terapia Antirretroviral de Alta Atividade , Broncopatias/diagnóstico , Tosse/etiologia , Infecções por HIV/complicações , Infecções por HIV/tratamento farmacológico , Síndrome Inflamatória da Reconstituição Imune/diagnóstico , Broncopatias/etiologia , Tosse/patologia , Infecções por HIV/patologia , Humanos , Síndrome Inflamatória da Reconstituição Imune/etiologia , Masculino , Pessoa de Meia-IdadeRESUMO
Relative expression of AKR1C family members and 5alphaR1 were determined using gene specific quantitative real-time PCR method. Both ARK1C1 and AKR1C2 were found significantly reduced in ovarian tumor tissues compared to ovarian normal tissues. In contrast, AKR1C3 and 5alphaR1 were kept the same transcriptional levels between ovarian tumor tissues and ovarian normal tissues. Immunohistochemical staining indicates that AKR1C1 shares the same localization with AKR1C3. Loss of ARK1C1 and AKR1C2 in ovarian cancerous tissues may enhance progesterone signaling in ovary itself by decreasing the ability of progesterone metabolism.
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20-Hidroxiesteroide Desidrogenases/classificação , 20-Hidroxiesteroide Desidrogenases/metabolismo , Regulação Enzimológica da Expressão Gênica , Ovário/enzimologia , Feminino , Humanos , Isoenzimas/metabolismo , Ovário/citologiaRESUMO
Epidemiologic data have implicated reproductive follicle-stimulating hormone (FSH) as a probable risk factor for ovarian cancer (OC) development. Although pituitary and sex hormones have been reported to regulate OC cell growth, no information is available on the influence of FSH on gene expression profiles during ovarian surface epithelial (OSE) cell proliferation. This study evaluated the effect of FSH treatment on cell proliferation of various OSE cell lines and gene expression profiles with FSH treatment. Follicle-stimulating hormone receptor (FSHR) was found at higher expression at both transcriptional and protein levels in ovarian cancerous tissues compared to normal tissues, and FSH was shown to promote cell growth in 3 OSE cell lines. Furthermore, it was also found that overexpression of FSHR in Chinese hamster ovary (CHO) cells leads to cell proliferation. Using cDNA MicroArray analysis on MCV152 cells with FSH treatment, 91 genes were found upregulated and 68 genes downregulated for more than 2-fold after FSH treatment. Most of the genes were related to metabolism, cell proliferation and oncogenes. Downregulated genes included tumor suppressor genes (RB1, BRCA1, BS69) and the genes related to cell proliferation control. Pathway analysis found that FSH activates certain important enzymes in sterol biosynthesis pathways. FSH-induced gene expression profiles on MCV152 cells support the standing hypothesis that FSH is a probable risk factor for ovarian cancerous development.