RESUMO
Although the widespread use of sewage sludge in developing countries is common, little is known about how sludge disposal can affect the microbial composition and diversity of tropical soils. We evaluated the effects of the sewage sludges of two types of anaerobic digestors differing, by the biological treatment they have undergone (uplow anaerobic sludge blanket and activated sludge digester), and two different disposal methods (surface and incorporated) on tropical soils. Samples were taken from topsoil (0-10 cm) and analyzed by amplifying the 16S rRNA genes to study the microbial community, and physicochemical analysis was performed concomitantly. The results indicated that, in general, sewage sludge amendment (SSA) significantly changed the tropical soil bacterial community by the sludge type and by application method. Moreover, the redundancy analysis diagram indicates that changes in soil chemical parameters over time due to SSA resulted in changes in the bacterial community's composition, increasing the population responsible for recycling nutrients in the soil.
Assuntos
Esgotos , Poluentes do Solo , Bactérias/genética , RNA Ribossômico 16S/genética , SoloRESUMO
The use of biofilters (working bed volume of 7.85 L) for the oxidation of CH4 at low concentrations (from 0.17%v/v to 3.63%v/v, typically in waste gas from anaerobic sewage treatment) was investigated and four empty bed residence times were tested (in min): 42.8, 29.5, 19.6, and 7.4. Mixtures of organic (composted leaves) and three non-organic materials (sponge-based material - SBM, blast furnace slag - BFS, and expanded vermiculite - ExpV) were used as packing media. Along 188 operational days after the steady state was reached (95 days for start-up), the CH4 mineralization decreased while the inlet loads gradually increased from 3.0 ± 0.8 gCH4 m(-3) h(-1) to 148.8 ± 4.4 gCH4 m(-3) h(-1). The biofilter packed with ExpV showed the best results, since the CH4 conversions decreased from 95.0 ± 5.0% to 12.7 ± 3.7% as a function of inlet concentration, compared to the other two biofilters (SBM and BFS) which showed CH4 conversions decreasing from 56.0 ± 5.4% to 3.5 ± 1.2% as a function of inlet concentration. The methanotrophic activity of biomass taken from ExpV biofilter was three times higher than the activity of biomass from the other two biofilters. Taken together, these results suggested that ExpV provides an attractive environment for microbial growth, besides the mechanical resistance provided to the whole packing media, showing the potential to its use in biofiltration of diffuse CH4 emissions.
Assuntos
Poluentes Atmosféricos/metabolismo , Filtração/métodos , Metano/metabolismo , Silicatos de Alumínio , Biodegradação Ambiental , Biomassa , Resíduos Industriais , Oxirredução , Folhas de Planta , Polietileno , Poliuretanos , SoloRESUMO
We investigated the microbial community in an up-flow anaerobic sludge blanket (UASB) reactor treating domestic wastewater (DW) during two different periods of organic loading rate (OLR) and food-to-microorganism (F/M) ratio. 16S rDNA clone libraries were generated, and quantitative real-time PCR (qPCR) analyses were performed. Fluctuations in the OLR and F/M ratio affected the abundance and the composition of the UASB prokaryotic community, mainly at the species level, as well as the performance of the UASB reactor. The qPCR analysis suggested that there was a decrease in the bacterial cell number during the rainy season, when the OLR and F/M ratio were lower. However, the bacterial diversity was higher during this time, suggesting that the community degraded more diversified substrates. The diversity and the abundance of the archaeal community were higher when the F/M ratio was lower. Shifts in the methanogenic community composition might have influenced the route of methane production, with methane produced by acetotrophic methanogens (dry season), and by hydrogenotrophic, methylotrophic and acetotrophic methanogens (rainy season). This study revealed higher levels of bacterial diversity, metabolic specialization and chemical oxygen demand removal efficiency of the DW UASB reactor during the rainy season.
Assuntos
Archaea/isolamento & purificação , Bactérias/isolamento & purificação , Reatores Biológicos/microbiologia , Biota , Compostos Orgânicos/análise , Esgotos/microbiologia , Águas Residuárias/microbiologia , Archaea/classificação , Archaea/genética , Bactérias/classificação , Bactérias/genética , Alimentos , Metano/metabolismo , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , Reação em Cadeia da Polimerase em Tempo Real , Estações do Ano , Análise de Sequência de DNA , Pesos e MedidasRESUMO
Brazil has become one of the epicentres of the COVID-19 pandemic, with cases heavily concentrated in large cities. Testing data is extremely limited and unreliable, which restricts health authorities' ability to deal with the pandemic. Given the stark demographic, social and economic heterogeneities within Brazilian cities, it is important to identify hotspots so that the limited resources available can have the greatest impact. This study shows that decentralised monitoring of SARS-CoV-2 RNA in sewage can be used to assess the distribution of COVID-19 prevalence in the city. The methodology developed in this study allowed the identification of hotspots by comprehensively monitoring sewers distributed through Belo Horizonte, Brazil's third largest city. Our results show that the most vulnerable neighbourhoods in the city were the hardest hit by the pandemic, indicating that, for many Brazilians, the situation is much worse than reported by official figures.
Assuntos
COVID-19 , Pandemias , SARS-CoV-2/isolamento & purificação , Esgotos/virologia , Brasil/epidemiologia , COVID-19/epidemiologia , Humanos , Prevalência , RNA ViralRESUMO
The encapsulation of microperoxidases (MPs) into molecular sieves with controlled pore size, such as the mesoporous silica MCM-41, represents a nanotechnology strategy to control the catalytic properties of MPs and mimic the enzymatic activity of hemoproteins. In this work, the ferric microperoxidase-11 (MP-11), obtained from trypsin-catalyzed hydrolysis of horse-heart cytochrome c, was entrapped in MCM-41, thus resulting in a catalyst (Fe(III)MP11MCM41) with catalase and monooxygenase properties. The entrapment of MP-11 inside MCM-41 was confirmed by elemental analysis and UV-visible spectrum, with a red shift in the Soret band indicating that the heme group was in a hydrophobic microenvironment. Similarly to catalase, the catalyst Fe(III)MP11MCM41 exhibited specificity for hydrogen peroxide to be converted to a high-valence oxidized intermediate, Compound II. Also mimicking catalase, the cleavage of hydrogen peroxide by MP11MCM41 resulted in O2 production detected by a Clark electrode. Phenol was able to act as reducing agent of MP11MCM41 Compound II leading to the completion of a peroxidase cycle, as confirmed by UV-visible spectrometry and EPR measurements. The analysis of the reaction products by high performance liquid chromatogram coupled to tandem mass spectrometry (HPLC/MS) revealed 2,4-dihydroxyphenol as the product of phenol oxidation by MP11MCM41. Therefore, in addition to catalase activity, the catalyst MP11MCM41 also displayed monooxygenase properties, which was possible because the MP-11 heme iron promotes homolytic cleavage of the hydrogen peroxide generating hydroxyl radicals. With such characteristics, MCM-41-entrapped MP-11 is a promising catalyst for nanobiotechnological devices.
Assuntos
Cristalização/métodos , Nanoestruturas/química , Peroxidases/química , Peroxidases/ultraestrutura , Fenol/química , Dióxido de Silício/química , Absorção , Catálise , Enzimas Imobilizadas/química , Teste de Materiais , Nanoestruturas/ultraestrutura , Nanotecnologia/métodos , Oxirredução , Tamanho da PartículaRESUMO
Understanding latitudinal variation in avian life-history traits has been a focus of many demographic studies around the world. However, we still know little about annual or intra-annual demographic variation within tropical regions or about how factors such as breeding season and precipitation influence demographic rates. In this study, we estimated intra-annual apparent survival of the White-lined Tanager (Tachyphonus rufus) using capture-mark-recapture data from northeastern Brazil. We tested whether survival varied seasonally (breeding vs. non-breeding), with rainfall, by age and residence status in our study area. Intra-annual apparent survival was correlated with the reproductive cycle, being lower during the breeding (0.65 ± 0.16 SE) vs. the non-breeding season (0.97 ± 0.05 SE). The annual apparent survival (~0.6) was relatively low for a tropical species. In both years, we observed highest abundance in spring (November, 3.1-3.7 birds/ha) and lowest abundance in autumn-winter periods (May-August, 1.1-1.4 bird/ha). The low survival during the breeding season probably reflects the trade-off between survival and reproduction and the cost of reproduction. Our findings represent an advance in the understanding of the demography of tropical birds because we did not find a predicted high annual apparent survival, and we elucidated some aspects of intra-annual variation in survival. Further exploration of latitudinal variation in demographic traits, especially in diverse, but poorly known habitats is needed to fully vet and develop life history theories.
Assuntos
Longevidade/fisiologia , Passeriformes/fisiologia , Reprodução/fisiologia , Distribuição Animal/fisiologia , Animais , Brasil , Feminino , Masculino , Chuva , Floresta Úmida , Estações do AnoRESUMO
We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.