Assuntos
Aeromonas/isolamento & purificação , Doenças dos Peixes/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Animais , Aquicultura , Argentina/epidemiologia , Proteínas de Bactérias/genética , Peixes-Gato , Surtos de Doenças/veterinária , Doenças dos Peixes/patologia , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/patologia , Proteínas Hemolisinas/genéticaRESUMO
The frequencies of several human platelet antigens (HPAs) vary between different populations and are a major determinant for the prevalence of HPA alloimmunization and its clinical associated entities. The aim of this study was to characterize the allele frequencies of seven HPA systems in two different ethnic groups from the Argentinean city of Rosario, the major population and a minority Amerindian group recently arrived from the north of the country, the Tobas. A total of 192 healthy unrelated individuals from blood donors and hospital staff from the Hospital Italiano Garibaldi and 27 unrelated Toba Amerindians were genotyped for HPA-1, -2, -3, -4, -5, -6 and -15 systems by polymerase chain reaction with sequence specific primers (PCR-SSP). The present data showed that the distribution of the HPA alleles among Argentineans from Rosario is quite similar to that reported among Europeans. The frequencies seen in Tobas, although limited by the small number of aboriginal samples studied, are similar to those reported for other Amerindians populations. Statistically significant differences were found for the genotype distribution of HPA-1, -3, -5 and -15 between both groups, indicating important differences in the potential risk of HPA alloimmunization associated to transfusion and pregnancy. The study of these polymorphisms represents the first step in the elucidation of pathological conditions that are underdiagnosed in our population. It allowed us to establish a panel of characterized blood donors necessary for the serological work out and as a source for compatible platelets transfusion.
Assuntos
Plaquetas/imunologia , Isoantígenos/sangue , Isoantígenos/genética , Argentina , Povo Asiático/genética , Doadores de Sangue , DNA/sangue , DNA/genética , Etnicidade/genética , Frequência do Gene , Genótipo , Humanos , População Branca/genéticaRESUMO
534 autoclaves from Rosario dentist offices were controlled by a method developed in our laboratory, that consists in: a) a procedures instruction; b) a survey ; c) a colorimetric control; d) a biological control. By this method it is possible to detect the mistake in the autoclave function by only one step. The results showed that 86.90% of the autoclaves lacked thermometers, 76.60% lacked manual thermostats, 83.33% were automatic and 58.80% did not sterilize. It can be concluded the necessity of a periodic control by this method, the importance of a commercial quality control of the furnaces and the urgency of continuous education over biosafety concepts.
Assuntos
Equipamentos Odontológicos/normas , Controle de Infecções/normas , Esterilização/métodos , Argentina , Bacillus subtilis/fisiologia , Colorimetria , Coleta de Dados , Equipamentos Odontológicos/estatística & dados numéricos , Desenho de Equipamento , Falha de Equipamento/estatística & dados numéricos , Controle de Qualidade , Esporos Bacterianos , Esterilização/instrumentação , Esterilização/normas , Termômetros/normasRESUMO
The present study evaluates the efficiency of the following decontaminating agents for the multiresistant, locally circulating bacterium Pseudomonas aeruginosa: glutaraldehyde 2%--makes A and B-, glutaraldehyde-formaldehyde; povidone-iodine-makes A, B and C-; sodium hypochloride; chloroxylenol--makes A and B-; and lapire chloride. The 9027 ATCC strain was used as a standard. A modification of the method of Kelsey and Sykes (1) was used to evaluate decontaminating efficiency. Highly satisfactory results were obtained with glutaraldehide 2% A and B, glutaraldehyde-formaldehyde and sodium hypochlorite. The results for povidone-iodine A, B and C were satisfactory but were unsatisfactory for chloroxylenol and lapirium chloride.
Assuntos
Desinfetantes de Equipamento Odontológico/farmacologia , Desinfetantes de Equipamento Odontológico/normas , Farmacorresistência Bacteriana , Formaldeído/farmacologia , Glutaral/farmacologia , Humanos , Povidona-Iodo/farmacologia , Pseudomonas aeruginosa/efeitos dos fármacos , Controle de Qualidade , Compostos de Amônio Quaternário/farmacologia , Hipoclorito de Sódio/farmacologia , Xilenos/farmacologiaRESUMO
534 autoclaves from Rosario dentist offices were controlled by a method developed in our laboratory, that consists in: a) a procedures instruction; b) a survey ; c) a colorimetric control; d) a biological control. By this method it is possible to detect the mistake in the autoclave function by only one step. The results showed that 86.90
of the autoclaves lacked thermometers, 76.60
lacked manual thermostats, 83.33
were automatic and 58.80
did not sterilize. It can be concluded the necessity of a periodic control by this method, the importance of a commercial quality control of the furnaces and the urgency of continuous education over biosafety concepts.
RESUMO
534 autoclaves from Rosario dentist offices were controlled by a method developed in our laboratory, that consists in: a) a procedures instruction; b) a survey ; c) a colorimetric control; d) a biological control. By this method it is possible to detect the mistake in the autoclave function by only one step. The results showed that 86.90
of the autoclaves lacked thermometers, 76.60
were automatic and 58.80
did not sterilize. It can be concluded the necessity of a periodic control by this method, the importance of a commercial quality control of the furnaces and the urgency of continuous education over biosafety concepts.
RESUMO
The present study evaluates the efficiency of the following decontaminating agents for the multiresistant, locally circulating bacterium Pseudomonas aeruginosa: glutaraldehyde 2
--makes A and B-, glutaraldehyde-formaldehyde; povidone-iodine-makes A, B and C-; sodium hypochloride; chloroxylenol--makes A and B-; and lapire chloride. The 9027 ATCC strain was used as a standard. A modification of the method of Kelsey and Sykes (1) was used to evaluate decontaminating efficiency. Highly satisfactory results were obtained with glutaraldehide 2
A and B, glutaraldehyde-formaldehyde and sodium hypochlorite. The results for povidone-iodine A, B and C were satisfactory but were unsatisfactory for chloroxylenol and lapirium chloride.
RESUMO
The present study evaluates the efficiency of the following decontaminating agents for the multiresistant, locally circulating bacterium Pseudomonas aeruginosa: glutaraldehyde 2
--makes A and B-, glutaraldehyde-formaldehyde; povidone-iodine-makes A, B and C-; sodium hypochloride; chloroxylenol--makes A and B-; and lapire chloride. The 9027 ATCC strain was used as a standard. A modification of the method of Kelsey and Sykes (1) was used to evaluate decontaminating efficiency. Highly satisfactory results were obtained with glutaraldehide 2
A and B, glutaraldehyde-formaldehyde and sodium hypochlorite. The results for povidone-iodine A, B and C were satisfactory but were unsatisfactory for chloroxylenol and lapirium chloride.