RESUMO
The acyclic nucleotide analogue (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(S)-HPMPA] is a potent and selective inhibitor of African swine fever virus (ASFV) replication. Using the DNA-DNA hybridization technique with plasmid pRPEL-2 as probe, we have shown that (S)-HPMPA exerts a specific, dose-dependent, inhibitory effect on viral DNA synthesis. Also, (S)-HPMPA inhibits the production of late viral proteins, especially IP-73, in ASFV-infected MS and Vero cells. When evaluated under the same experimental conditions, phosphonoacetic acid (PAA) also caused an inhibition of viral DNA and late viral protein synthesis but only so at a concentration which was 10- to 20-fold higher than that required for (S)-HPMPA.
Assuntos
Adenina/análogos & derivados , Vírus da Febre Suína Africana/genética , Antivirais/farmacologia , Replicação do DNA/efeitos dos fármacos , Iridoviridae/genética , Organofosfonatos , Compostos Organofosforados , Adenina/farmacologia , Vírus da Febre Suína Africana/efeitos dos fármacos , Animais , Linhagem Celular , DNA Viral/efeitos dos fármacos , Humanos , Ácido Fosfonoacéticos/farmacologia , Células VeroRESUMO
The late cytoplasmic phases of African swine fever virus (ASFV) morphogenesis in monkey kidney cells have been studied by transmission electron microscopy, focusing attention on the synthesis of viral envelopes. Morphogenesis was studied after reversible cycloheximide blockage of monkey kidney cells infected with ASFV. ASFV appears to synthesize its external and internal envelopes within the cellular cytoplasm, at the same time as the capsid is formed, with intracellular and extracellular virions showing similar structure and polypeptide composition.