A sportomics soccer investigation unveils an exercise-induced shift in tyrosine metabolism leading to hawkinsinuria.

Tyrosine metabolism has an intense role in the synthesis of neurotransmitters. Our study used an untargeted, sportomics-based analysis of urine samples to investigate changes in metabolism during a soccer match in 30 male junior professional soccer players. Samples were collected before and after the match and analyzed using liquid chromatography and mass spectrometry. Results showed significant changes in tyrosine metabolism. Exercise caused a downregulation of the homogentisate metabolites 4-maleylacetoacetate and succinylacetone to 20% (p = 4.69E-5) and 16% (p = 4.25E-14), respectively. 4-Hydroxyphenylpyruvate, a homogentisate precursor, was found to be upregulated by 26% (p = 7.20E-3). The concentration of hawkinsin and its metabolite 4-hydroxycyclohexyl acetate increased ~six-fold (p = 1.49E-6 and p = 9.81E-6, respectively). Different DOPA metabolism pathways were also affected by exercise. DOPA and dopaquinone increased four-to six-fold (p = 5.62E-14 and p = 4.98E-13, respectively). 3-Methoxytyrosine, indole-5,6-quinone, and melanin were downregulated from 1 to 25%, as were dopamine and tyramine (decreasing to up to 5% or 80%; p= 5.62E-14 and p = 2.47E-2, respectively). Blood TCO2 decreased as well as urinary glutathione and glutamate (40% and 10% respectively) associated with a two-fold increase in pyroglutamate. Our study found unexpected similarities between exercise-induced changes in metabolism and the inherited disorder Hawkinsinuria, suggesting a possible transient condition called exercise-induced hawkinsinuria (EIh). Additionally, our research suggests changes in DOPA pathways may be involved. Our findings suggest that soccer exercise could be used as a model to search for potential countermeasures in Hawkinsinuria and other tyrosine metabolism disorders.

Comparison between traditional strength training and complex contrast training on soccer players.

BACKGROUND: The purpose of this study was to compare traditional strength training (TST) versus complex and contrast training (CCT) on sprint, change of direction speed (COD) and squat jump (SJ) in young male soccer players. METHODS: Twenty-two soccer players (age: 18.4±0.4 years, body mass: 70.2±9.1 kg, height: 179.9±7.5 cm), were randomly assigned to one of two groups: TST (N.=12) and CCT (N.=10). The study was conducted using a randomized experimental design over an eight-week period. The participants assigned to the CCT group performed high-power exercises paired with high-velocity exercises. The participants assigned to the TST group performed resistance exercises in a straight-set forma. During the study period, sprint tests for 5, 10, 20 and 30 m split times, COD and SJ were applied. A two-way ANOVA was applied, and the alpha level was P<0.05. RESULTS: The results demonstrated that the CCT regimen elicited significant within-group differences in 5 m sprint time (1.032 s to 0.997 s, pre- and postintervention, respectively, effect size (ES) = -0.5, medium; P=0.04), COD (5.963 s to 5.639 s, pre- and postintervention, respectively, ES -2.7, large; P<0.001) and SJ (30.9 cm to 34.4 cm, pre- and postintervention, respectively, ES =0.8, large; P<0.001). Conversely, the TST did not elicit significant within-group differences for any of the dependent variables. No differences were found between groups at the post-test time point. CONCLUSIONS: In conclusion, the CCT protocol could be used to improve sprint, COD and SJ in male soccer players.

Evaluation of physical activity habits in patients with posttraumatic stress disorder.

OBJECTIVE: In this study, we present data from a survey that aimed to assess the physical activity habits of adult Brazilian patients with Posttraumatic Stress Disorder. METHOD: Fifty male and female patients with Posttraumatic Stress Disorder participated in this study. The mean age at onset was 37+/-12 years, and the mean time between diagnosis and follow-up was 3.6+/-4.2 years. RESULTS: Substantial changes in physical activity habits were observed following the onset of PTSD. While more than half of the patients participated in physical activities prior to Posttraumatic Stress Disorder onset, there was a significant reduction in their participation afterwards. The justifications for stopping physical activities or sport participation were lack of time and lack of motivation. DISCUSSION: Several studies have shown that physical exercise decreases reverts symptoms of psychiatric disorders such as depression, anxiety and social isolation. We could therefore hypothesize that patients with Posttraumatic Stress Disorder who exercise should experience the same benefits. CONCLUSION: Our findings demonstrated that patients with Posttraumatic Stress Disorder have low levels of participation in sports or physical activities.

Comparison between traditional strength training and complex contrast training on repeated sprint ability and muscle architecture in elite soccer players.

BACKGROUND: The purpose of this study was to compare traditional strength training (TST) and complex contrast training (CCT) on the repeated-shuttle-sprint ability (RSSA), the countermovement squat jump (CMJ) height, the one repetition maximum (1RM) at squat on the Smith machine, and on muscle architecture in young, male elite soccer players. METHODS: Twenty-two soccer players (mean age 18.4±0.4 years; mean weight 70.2±9.1 kg; mean height 179.9±7.5 cm) who belonged to the under-20 age group were randomly assigned into two groups: CCT (N.=10) or TST (N.=12). During the study period, the soccer players trained with CCT through power exercises performed before high-velocity exercises and TST based on a set-repetition format through daily, undulatory periodization. RESULTS: After statistical analysis (P<0.05), the results demonstrated that the specific CCT regimen provided a significant improvement in the RSSA percent decrement (moderate effect size), CMJ (large effect size) and 1RM ability (large effect size). However, the TST promoted significant changes in 1RM (large effect size) and a significant increase in the muscle thickness of the vastus intermedius (moderate effect size). CONCLUSIONS: The CCT protocol could be used to improve the RSSA parameters, CMJ and 1RM, and the TST developed dynamic strength and muscle growth. Coaches can choose either CCT or TST protocols according to the needs of their soccer players.

Indicadores de qualidade em centro cirúrgico especializado em dermatologia / Indicadores de calidad en centro quirúrgico especializado en dermatología / Quality indicators at a surgical center specialized in dermatology

Objetivo: identificar e caracterizar os indicadores de qualidade mais frequentes utilizados em um Centro Cirúrgico público especializado em dermatologia. Método: estudo transversal seccional, pelo qual fez-se um levantamento, com registros diários, dos mais frequentes indicadores entre os 20 existentes na unidade, no período de agosto de 2015 a julho de 2016. Considerou-se: médias, medianas, valores mínimos/máximos, desvios padrões, frequências simples e percentuais. O valor de p<0,05 foi considerado como nível de significância. Resultados: das 595 cirurgias eletivas, destacaram-se as ambulatoriais (87,9%), as dermatológicas (81,3%), em pacientes do sexo masculino (52,6%), com média de 54,9 anos. Os cinco indicadores mais frequentes foram: cirurgias suspensas (17,6%); registros de enfermagem incompletos (14%); absenteísmo (6,5%); infecção em sítio cirúrgico em cirurgias limpas (2,7%) e acidentes de trabalho (2,2%). Conclusões: a identificação e a caracterização dos indicadores de qualidade proporcionaram visibilidade aos processos locais, viabilizando a tomada de decisões e o aperfeiçoamento dos serviços prestados(AU).

Objetivo: Identificar y caracterizar los indicadores de calidad más frecuentemente utilizados en un centro quirúrgico público especializado en dermatología. Método: Estudio transversal seccional, requiriendo relevamiento, con registros diarios de los indicadores más frecuentes entre los 20 existentes en la unidad, de agosto de 2015 a julio de 2016. Se consideraron: promedios, medianas, valores mínimos/máximos, desvíos estándar, frecuencias simples y porcentuales. Nivel de significancia establecido en p<0,05. Resultados: De 595 cirugías electivas, se destacaron las ambulatorias (87,9%), las dermatológicas (81,3%), en pacientes masculinos (52,6%) con promedio de 54,9 años. Los cinco indicadores más frecuentes fueron: cirugías suspendidas (17,6%); registros de enfermería incompletos (14%); ausentismo (6,5%), infección en sitio quirúrgico en cirugías limpias (2,7%) y accidentes laborales (2,2%). Conclusiones: La identificación y caracterización de indicadores de calidad otorgó visibilidad a los procesos locales, haciendo posible la toma de decisiones y el mejoramiento de los servicios brindados(AU).

Objective: to identify and characterize the most frequent quality indicators used at a public surgical center specialized in dermatology. Methods: cross-sectional study in which a survey consisting of daily records of the most frequent quality indicators among the 20 existing at the unit was carried out between August 2015 and July 2016. Mean, median minimum and maximum values, standard deviations, and absolute and percentage frequencies were obtained. A p-value lower than 0.05 (p<0.05) was considered as the level of significance. Results: in a total of 595 elective surgeries, outpatient (87.9%), dermatologic (81.3%), and those performed in men (52.6%) with an average age of 54.9 years stood out. The five most frequent indicators were: cancelled surgeries (17.6%), incomplete nursing records (14%), absenteeism (6.5%), infection in surgical sites in clean surgeries (2.7%), and occupational accidents (2.2%). Conclusions: identifying and characterizing quality indicators give visibility to local processes and enable decision-making and improvement of the offered services(AU).

Intravenous toxicity of fructose-1,6-bisphosphate in rats.

Fructose-1,6-bisphosphate (FBP) is a bisphosphorilated sugar with a protective action against events that lead to cellular damage. The toxicity of the drug was assessed when administered intravenously in Wistar rats in doses of between 250 and 4000 mg/kg. Ionic calcium, total calcium, inorganic serum phosphate and the electrocardiographic profile of these animals were assessed. The lethal dose (LD(50)) was established by means of PROBIT processing. There was no reduction in the levels of total calcium, with the administration of increased doses of FBP, although there was a significant reduction in the levels of ionic calcium in those groups that received 250 mg/kg and over. The serum phosphate showed a significant statistical increase in those groups that received 750 mg/kg and over. The LD(50) obtained in 24 h was 1068 mg/kg. Though it was not possible to elucidate the toxic mechanism of FBP, the electrocardiogram (ECG) showed that all the rats died of cardiac arrest.

Conhecimento dos Graduandos de Enfermagem sobre o uso de Tecnologias Informatizadas / Knowledge of Nursing Undergraduate about Technology use Computerized / Conocimiento de Licenciatura de Enfermería sobre uso de Tecnología Informatizado

Objetivo: Identificar o conhecimento dos graduandos de enfermagem sobre o uso de sistemas informatizados na prática do cuidado. Método: Trata-se de um estudo de caso, realizado em uma Universidade privada da região do Alto Tietê, sendo a amostra obtida por conveniência e constituída de 50 graduandos de Enfermagem. Resultados: Identificou-se que 94% dos participantes utilizam o computador com frequência no seu cotidiano, além disso, 100% deles tem acesso à internet. No entanto, 58% não conhecem nenhum tipo de tecnologia informatizada na área da saúde. Ressalta-se que 48% afirmaram não estar preparados para utilizar os recursos existentes e dentre as principais dificuldades citadas estão à falta de conhecimento (54%) e a falta de treinamento (13%). Conclusão: Ainda existem barreiras no conhecimento dos graduandos para a realidade do mercado tecnológico, mas estas podem ser quebradas por meio de treinamentos constantes.

Objective: To identify the knowledge of nursing students on the use of computerized systems in nursing practice. Method: This is a case study, carried out at a private university in the Alto Tietê region, the sample being obtained for convenience and made up of 50 Nursing undergraduates. Results: It was found that 94% of participants use the computer frequently, in addition, 100% of them have Internet access. However, 58% doesn't knew any type of computerized technology in healthcare. It is noteworthy that 48% said they were not prepared to use computerized resources, and among the main difficulties cited are lack of knowledge (54%) and lack of training (13%). Conclusion: The barriers still exist in the knowledge of the students to reality technology market, but these barriers can be broken with constant training.

Objetivo: Identificar el conocimiento de los estudiantes de enfermería en el uso de sistemas informáticos en la práctica de enfermería. Método: Se trata de un caso de estudio en una universidad privada de la región de Alto Tietê, y la muestra obtenida por conveniencia y se compone de 50 estudiantes de enfermería. Resultados: Se encontró que el 94% de los participantes utilizar el equipo con frecuencia, además, el 100% de ellos tiene acceso a Internet. Sin embargo, el 58% no sabe cualquier tipo de tecnología computarizada en la asistencia sanitaria. Es de destacar que el 48% dijo que no estaban dispuestos a utilizar los recursos computarizados, y entre las principales dificultades mencionadas son la falta de conocimiento (54%) y la falta de formación (13%). Conclusión: Por lo tanto, se constató que todavía existen barreras en el conocimiento de los estudiantes al mercado la tecnología de realidad, pero estas barreras se puede romper a través de constante formación.

Comparação entre diferentes modelos de periodização sobre a força e espessura muscular em uma sequência dos menores para os maiores grupamentos musculares / Comparison between different periodization models on muscular strength and thickness in a muscle group increasing sequence

INTRODUÇÃO: Estudos comparando modelos de periodização em sequências de treinamento resistido (TR) realizadas do menor para o maior grupo muscular sobre as alterações musculares em indivíduos destreinados em TR são escassos. OBJETIVO: Comparar o efeito da periodização ondulatória (PO) e da periodização linear (PL) sobre a força máxima e hipertrofia muscular em uma sequência de execução dos exercícios dos menores para os maiores grupamentos musculares. MÉTODOS: Vinte e nove homens não experientes em (TR) foram distribuídos aleatoriamente em três grupos: PO (n =10), PL (n = 13) e grupo controle (GC, n = 9). Os indivíduos realizaram o teste de uma repetição máxima (1RM) nos exercícios rosca bíceps (RB), rosca tríceps (RT), puxada aberta (PA) e supino reto (SR), contração isométrica voluntária máxima (CIVM) e espessura muscular (EM) para flexores de cotovelo (FC) e extensores de cotovelo (EC) antes e após o período de 12 semanas de treinamento. O grupo PO variou o volume e a intensidade do treinamento diariamente, e o grupo PL a cada quatro semanas. O GC não realizou TR. Foi realizada uma ANOVA de dois caminhos com medidas repetidas, e cálculo do tamanho do efeito (TE), nas cargas obtidas para analisar o efeito do tratamento sobre as variações pré- e pós-período de TR. RESULTADOS: Os principais achados do estudo foram: 1) o grupo PO apresentou maior TE para 1RM dos exercícios RT e RB e para EM dos FC e EC quando comparado ao grupo PL; 2) não houve diferença no TE para os exercícios SR e PA que finalizavam a sessão. CONCLUSÃO: Ambos os modelos de periodização adotados foram eficientes para promover aumentos de força e hipertrofia muscular. Contudo, segundo o cálculo do TE, a PO promoveu maior incremento da força máxima nos exercícios que iniciaram a sessão e hipertrofia muscular.

INTRODUCTION: Studies comparing periodization models in sequences that begin with small muscle group and progressed toward large muscle group in untrained subjects in resistance training are scarce. OBJECTIVE: The purpose of this study was to compare the effects of ondulatory periodization and linear periodization models on maximum strength and muscular hypertrophy in a muscle group increasing exercise sequence. METHODS: Twenty-nine men with no experience in RT were randomly assigned into three groups: ondulatory periodization (OP, n = 10), linear periodization (LP, n = 13), and control group (CG, n = 9). The individuals performed 1RM tests in four exercises: biceps curl (BC), triceps extension (TE), lat pull down (LPD) and bench press (BP) and evaluations of maximum voluntary isometric contraction (MVIC), muscle thickness of elbow flexors (EF) and elbow extensors (EE) before and after the 12 weeks of training were carried out. The OP group varied in volume and intensity on a daily basis, while LP group varied every four weeks. The CG did not perform ST. A two-way ANOVA with repeated measures and the effect size (ES) were used to analyze muscle thickness, 1RM load improvement in each of the four exercises and the MVIC between groups. RESULTS: The major findings of this study were: 1) OP showed major ES for 1RM of BC and TE and for muscle thickness of EF and EE when compared with LP. 3) The ES data did not show significant differences for BP and LPD which finished the training session. CONCLUSIONS: We conclude that both periodization models were efficient at improving strength gains and muscular growth. However, ES data show that OP promotes major gains in strength for exercises that are positioned at the beginning of the session and hypertrophy.

Software para mapeamento dos riscos de Úlcera por Pressão, queda e flebite / Software for mapping of the risks of Pressure Ulcer, fall and phlebitis / Software de mapas de riesgo de Úlcera por Presión, caídas y flebitis

Desenvolver um software de mapeamento e classificação dos riscos de úlcera por pressão, queda e flebite para o auxílio nas ações preventivas. Método: Foi selecionado como recurso para desenvolvimento de software a linguagem Java, por meio da utilização do NetBeans IDE 6.9.1. O banco de dados foi desenvolvido em MySQL. O sistema foi desenvolvido em duas etapas: a prototipação, abordada como fase de definição e o desenvolvimento do software propriamente dito. Resultados: Dessa forma, o software ficou caracterizado com as seguintes funções: Novo Paciente, Revalidar Riscos, Saída do Paciente, Risco por Paciente, Filtrar Fatores de Risco e Mapa de Risco. Conclusão: Com esse estudo foi possível desenvolver uma ferramenta capaz de realizar a avaliação de risco de uma forma mais ágil e direcionada, o que possibilitará ao enfermeiro uma melhor abordagem em relação à prevenção desses eventos...

To develop a software of mapping and classification of the risks of pressure ulcer, fall and phlebitis to help in preventive actions. Methods: Was selected as a resource for developing the software the Java language, by using the NetBeans IDE 6.9.1. The database was developed in MySQL. The system was developed in two stages: prototyping, addressed as the definition phase and the development of the software itself. Results: So, the software was characterized with the following functions: New Patient, Revalidate Risk, Out of Patients, Patient Risk, Filter Risk Factors and Risk Map. Conclusions: With this study it was possible to develop a tool able to performing the risk assessment in a more agile and focused, which will enable to the nurse a better approach towards prevention of these events...

Desarrollar un software de mapas y clasificación de riesgo de úlcera por presión, caídas y flebitis para ayudar en las acciones preventivas. Método: Fue seleccionado como un recurso para el desarrollo de software de Java a través del uso de NetBeans IDE 6.9.1. La base de datos fue desarrollada en MySQL. El sistema fue desarrollado en dos fases: creación de prototipos, se dirigió a la fase de definición y desarrollo del software en sí. Resultados: De este modo, el software se ofrece con las siguientes funciones: Nuevo Paciente, Revalidar Riesgos, Salida de los Pacientes, Riesgo por Paciente, Filtrar Factores de Riesgo y Mapa de Riesgos. Conclusiones: Con este estudio, fue posible desarrollar una herramienta capaz de realizar la evaluación de riesgos de una manera más ágil y enfocada, que permitirá a las enfermeras una mejor abordaje en relación a la prevención de eses eventos...

Evaluation of physical activity habits in patients with posttraumatic stress disorder

OBJECTIVE: In this study, we present data from a survey that aimed to assess the physical activity habits of adult Brazilian patients with Posttraumatic Stress Disorder. METHOD: Fifty male and female patients with Posttraumatic Stress Disorder participated in this study. The mean age at onset was 37±12 years, and the mean time between diagnosis and follow-up was 3.6±4.2 years. RESULTS: Substantial changes in physical activity habits were observed following the onset of PTSD. While more than half of the patients participated in physical activities prior to Posttraumatic Stress Disorder onset, there was a significant reduction in their participation afterwards. The justifications for stopping physical activities or sport participation were lack of time and lack of motivation. DISCUSSION: Several studies have shown that physical exercise decreases reverts symptoms of psychiatric disorders such as depression, anxiety and social isolation. We could therefore hypothesize that patients with Posttraumatic Stress Disorder who exercise should experience the same benefits. CONCLUSION: Our findings demonstrated that patients with Posttraumatic Stress Disorder have low levels of participation in sports or physical activities.

O antígeno prostático específico e sua importância na decisão clínica no câncer de próstata / The prostatic specific antigen and its importance in the clinical decision in the prostate cancer

O câncer de próstata (CaP) representa um problema de saúde pública de proporções cada vez mais importantes. É uma das neoplasias mais frequentes nos homens e representa uma das principais causas de morte na população. A busca de métodos diagnósticos para as neoplasias é um constante objetivo clínico laboratorial. Exame físico, métodos de imagem e dosagens laboratoriais compreendem o conjunto de auxílio diagnóstico no CaP. O antígeno prostático específico (PSA) é produzido pelo tecido prostático normal, bem como, em casos de Hiperplasia Prostática Benigna (HPB) e no CaP. Sua dosagem constitui importante método laboratorial na prática médica, uma vez que se apresenta com grande valor no rastreamento, diagnóstico e acompanhamento dos casos de CaP. O entendimento do seu papel pelo farmacêutico-bioquímico, bem como, a interação com a equipe clínica mostra-se promissor para influenciar as decisões diagnóstico-terapêuticas.

Prostate cancer represents a public health concern whose proportions are becoming increasingly important. It is one of the most common neoplasia among men and it represents one of the main causes of death in the population. The search for diagnostic methods for this neoplasia has been a constant laboratory clinic target. Physical checking, imaging methods and laboratory dosages compose the range of diagnostic support when dealing with prostate cancer...

Prevalência de enteroparasitos em moradores de vilas periféricas de Porto Alegre, RS / Survey of intestinal parasites among inhabitants of Porto Alegre Metropolitan area

Realizou-se um trabalho integrado de pesquisa e extensão comunitária com o objetivo de determinar a prevalência das enteroparasitoses na vila Fátima (Mato Sampaio), Campo da Tuca e Vila dos Papeleiros, vilas periféricas de Porto Alegre. este estudo foi realizado durante os anos de 1999 e 2002 em uma população de 594 pessoas, todas residentes nestas vilas. As amostras fecais foram examinadas pela técnica de Hoffman, Pons e Janer. O maior percentual geral de infecção obtido para nematóides foi de 39,5% (235) para Ascaris lumbricoides e, entre os protozoários, a Giardia lamblia com 18,0% (107). As altas taxas de enteroparasitoses entre crianças e adultos jovens moradores destas vilas mostrou o baixo nível sócio-econômico, a falta de escolaridade e a inexistência de saneamento básico.