Non-specific immunity promotion in response to garlic extract supplemented diets in female Guppy (Poecilia reticulata).

In the present study, the effect of aqueous garlic (Allium sativum) extract on skin mucus immune parameters of Poecilia reticulata was evaluated. A total of 240 P. reticulate juveniles, weighing 0.013 ± 0.001 g, were randomly stocked in 12 experimental glass tanks at a density of 30 fish per tank. The fish were fed with diets supplemented with garlic extract at the concentrations of 0, 0.10, 0.15, and 0.20 ml kg-1 of diet three times a day at a ratio of 2.5% of their body weight for 80 days. Results of the study showed that, lysozyme activity increased significantly in the skin mucus of garlic-treated Guppy (P < 0.05). Values of ACH50 significantly increased (P < 0.05) in fish by increasing concentration of garlic extract from 0 to 0.15 ml kg-1 in diet. ACH50 also showed a significant declining trend (P < 0.05) by increasing concentration of garlic extract from 0.15 to 0.20 ml kg-1. Total Ig content was enhanced significantly in 0.15 and 0.20 ml kg-1 treatments as well. ALP activity significantly increased in garlic-fed treatments compared to the control. Inclusion of garlic extract in diet was found to have no significant effect on final body weight and weight gain. Results revealed that, administration of 0.15 mL of garlic extract per kg feed is suggested to obtain optimal skin mucus immunity in P. reticulata.

Microleakage of Er:YAG laser and dental bur prepared cavities in primary teeth restored with different adhesive restorative materials.

The purpose of this study was to evaluate and compare the effect of erbium:yttrium-aluminum-garnet (Er:YAG) laser irradiation and conventional dental bur cavity preparation on in vitro microleakage of class V cavities restored with different adhesive restorative materials and two types of self-etching adhesives in primary teeth. Standard class V cavities were prepared on 80 extracted primary, and the teeth were randomly divided into eight subgroups prepared either by dental bur or Er:YAG laser irradiation and then restored with self-cured glass ionomer (GI), resin-modified glass ionomer (RMGI), resin composite and Clearfil SE Bond (two-step self-etching adhesive), and resin composite and Clearfil S3 Bond (one-step self-etching adhesive). Restorations were finished and stored in distilled water at 37 °C for 24 h and then subjected to thermocycling. All the teeth were sealed with nail varnish, placed in a silver nitrate solution, and then vertically cut in a buccolingually direction. Subsequently, the specimens were evaluated for gingival and occlusal microleakage using a stereomicroscope. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. Wilcoxon test was used for comparing occlusal microleakage with gingival microleakage at p < 0.05. A higher degree of occlusal and gingival microleakage values for the teeth restored with GI or RMGI was obtained by both preparation methods compared with that of resin composites and the two self-etching primers. Er:YAG laser irradiation resulted in a significantly higher degree of microleakage only at the gingival margins for teeth restored with GI or RMGI, or composite and Clearfil S3 Bond compared with the bur preparation. The Er:YAG laser-prepared teeth restored with composite and Clearfil SE Bond demonstrated a better marginal seal on occlusal and gingival margins compared with that of bur-prepared cavities. The degree of microleakage in class V cavities was affected by the type of adhesive restorative materials, type of self-etching adhesive, cavity margin location, and tooth preparation method either by Er:YAG laser or dental bur.

The effect of erbium family laser on tensile bond strength of composite to dentin in comparison with conventional method.

The purpose of this study was to evaluate the effect of Er:YAG and Er,Cr:YSGG laser on tensile bond strength of composite resin to dentine in comparison with bur-prepared cavities. Fifteen extracted caries-free human third molars were selected. The teeth were cut at a level below the occlusal pit and fissure plan and randomly divided into three groups. Five cavities were prepared by diamond bur, five cavities prepared by Er:YAG laser, and the other group prepared by Er,Cr:YSGG laser. Then, all the cavities were restored by composite resin. The teeth were sectioned longitudinally with Isomet and the specimens prepared in dumbbelled shape (n = 36). The samples were attached to special jigs, and the tensile bond strength of the three groups was measured by universal testing machine at a speed of 0.5 mm/min. The results of the three groups were analyzed with one-way ANOVA and Tamhane test. The means and standard deviations of tensile bond strength of bur-cut, Er:YAG laser-ablated, and Er,Cr:YSGG laser-ablated dentine were 5.04 ± 0.93, 13.37 ± 3.87, and 4.85 ± 0.93 MPa, respectively. There is little difference in tensile bond strength of composite resin in Er,Cr:YSGG lased-prepared cavities in comparison with bur-prepared cavities, but the Er:YAG laser group showed higher bond strength than the other groups.

Clinical Evaluation for Space Maintainer after Unilateral Loss of Primary First Molar in the Early Mixed Dentition Stage.

Background and Objectives: Controversy exists regarding the need for a space maintainer after early unilateral loss of a primary first molar. This study aimed to assess the need for a space maintainer after unilateral loss of a primary first molar in the early mixed dentition period. Materials and Methods. In this cross-sectional study, fifty children between 6 and 8 years who had lost a primary first molar unilaterally later than 6 months ago were randomly selected. Midline deviation, molar and canine relationships at both sides, facial growth pattern, and the amount of space loss were all assessed. Data were analyzed using SPSS version 25 via the one-sample t-test, paired t-test, and linear regression (alpha = 0.05). Results. The mean amount of space loss was 1.36 ± 0.78 mm (1.32 mm in the maxilla and 1.40 mm in the mandible). Time since tooth extraction and facial pattern had significant correlations with space loss (P < 0.05). Conclusion. In this particular age group, it is imperative to precisely assess the related factors such as the facial pattern and time since tooth extraction to decide about the placement of a space maintainer for a prematurely lost primary first molar.

The Prevalence and Correlates of Fissured Tongue Among Outpatients in Andkhoy City, Afghanistan: A Cross-Sectional Study.

BACKGROUND: Fissured tongue is a common manifestation of the tongue, marked by the presence of multiple prominent grooves or fissures on the dorsal surface of the tongue. However, there is a lack of studies focusing on the prevalence and factors associated with fissured tongue among patients attending an outpatient clinic in Afghanistan. AIM: The purpose of the current study was to determine the prevalence and factors associated with fissured tongue and its effects on the general health of adult outpatients in Afghanistan. PATIENTS AND METHODS: The cross-sectional survey was conducted among outpatient populations in Andkhoy City, Afghanistan, between September 2019 and December 2019. A convenience technique was applied by including 1182 patient aged 18-80 years. Socio-economic status, smoking, nass use (smokeless tobacco use) and medical data were collected using face-to-face interviews. We used logistic regression analysis to identify factors associated with fissured tongue. A convenience technique was applied by including patient aged 18-80 years. RESULTS: The studied population consisted of 1182 participants, of whom 573 (48.5%) were males and 609 (51.5%) females. The prevalence of fissured tongue was 27.2% (95% CI: 24.7-29.9%) with males having significantly higher prevalence than females (47.5%, 95% CI: 43.3-51.6% versus 8.2%, 95% CI: 6.2-10.7%, p<0.001). Male participants (OR=7.1, 95% CI: 4.8-10.3), diabetes mellitus (OR=1.6, 95% CI: 1.1-2.3) and smokeless tobacco use (OR=12.0, 95% CI: 8.1-17.6) were the only variables independently associated with fissured tongue. CONCLUSION: This study suggested that there was a high rate of fissured tongue among an outpatient clinic in Andkhoy, Afghanistan. Male gender, diabetes mellitus, and nass consumption were associated with fissured tongue. Therefore, these factors might usefully be targeted in local health promotion, prevention and early intervention programs.

Comparison of different diagnostic techniques in detecting smooth surface caries in primary molars using the histological gold standard: An in vitro study.

OBJECTIVE: This in vitro study evaluated the caries status of the smooth surfaces of primary molars using the DIAGNOdent pen, Vistacam iX, and Bitewing radiography as compared to histologic assessment. METHODS: 68 primary molars were selected, and their mesial or distal caries status were determined using DIAGNOdent pen, Bitewing radiography, and Vistacam iX. To achieve reference standards, the teeth were sectioned and directly assessed by a stereomicroscope. The Mc Nemar test was used to compare the sensitivity, specificity, and accuracy of the methods. The spearman rank correlation coefficient (r) was also determined to compare the extent of caries with histology. The statistical significance level was set at α = 0.05. RESULTS: Regarding D1 as a cut-off point for histological assessments, sensitivity, specificity, and accuracy of DIAGNOdent pen were 82 %, 75 %, and 80 % while the values were 63 %, 100 % and 62 % for bitewing radiography and 56 %, 100 % and 59 % for Vistacam iX. Regarding D3 as a cut-off point for histological analysis; Sensitivity, specificity, and accuracy rates of DIAGNOdent pen were 71 %, 86 % and 80 % while these values were 71 %, 73 % and 72 % for bitewing radiography and 50 %, 78 % and 67 % for Vistacam iX. CONCLUSION: In conclusion, the DIAGNOdent pen shows the highest performance and accuracy to detect smooth surface caries both at D1 and D3 among the three methods. Although VistaCam iX and bitewing radiography showed better performance at D3 compared to D1, their performances were lower than the DIAGNOdent pen.