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J Cell Sci ; 129(1): 39-50, 2016 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-26567220

RESUMO

MicroRNAs (miRNAs) play important roles in the development of skeletal muscle. In our previous study, expression of miR-195 and miR-497 were shown to be upregulated during muscle development in pigs. In this study, we investigated the roles of these two miRNAs in myogenesis and analyzed their transcriptional regulation. Our results showed that miR-195 and miR-497 were upregulated during muscle development and myoblast differentiation. Moreover, miR-195 and miR-497 inhibited proliferation but not differentiation in C2C12 cells. Further investigation revealed that Igf1r, Insr, Ccnd2 and Ccne1 were directly targeted by miR-195 and miR-497 in myoblasts. In addition, we confirmed that miR-195 and miR-497, which shared the similar expression profiling, were negatively regulated by nuclear factor κB (NF-κB) in both myoblasts and skeletal muscle tissue. Our data illustrate that the signaling pathway NF-κB-miR-195/497-Igf1r/Insr-Ccnd2/Ccne1 plays important roles in myogenesis. Our study provides novel evidence for the roles of miR-195 and miR-497 in muscle development.


Assuntos
Ciclina D2/genética , Ciclina E/genética , MicroRNAs/metabolismo , Mioblastos/citologia , NF-kappa B/metabolismo , Receptor IGF Tipo 1/genética , Receptor de Insulina/genética , Animais , Sequência de Bases , Sítios de Ligação , Diferenciação Celular , Linhagem Celular , Proliferação de Células , Ciclina D2/metabolismo , Ciclina E/metabolismo , Camundongos Endogâmicos BALB C , MicroRNAs/genética , Dados de Sequência Molecular , Desenvolvimento Muscular , Mioblastos/metabolismo , Regiões Promotoras Genéticas/genética , Ligação Proteica , Receptor IGF Tipo 1/metabolismo , Receptor de Insulina/metabolismo , Regulação para Cima
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