SCARECROW-LIKE28 modulates organ growth in Arabidopsis by controlling mitotic cell cycle exit, endoreplication, and cell expansion dynamics.

The processes that contribute to plant organ morphogenesis are spatial-temporally organized. Within the meristem, mitosis produces new cells that subsequently engage in cell expansion and differentiation programs. The latter is frequently accompanied by endoreplication, being an alternative cell cycle that replicates the DNA without nuclear division, causing a stepwise increase in somatic ploidy. Here, we show that the Arabidopsis SCL28 transcription factor promotes organ growth by modulating cell expansion dynamics in both root and leaf cells. Gene expression studies indicated that SCL28 regulates members of the SIAMESE/SIAMESE-RELATED (SIM/SMR) family, encoding cyclin-dependent kinase inhibitors with a role in promoting mitotic cell cycle (MCC) exit and endoreplication, both in response to developmental and environmental cues. Consistent with this role, mutants in SCL28 displayed reduced endoreplication, both in roots and leaves. We also found evidence indicating that SCL28 co-expresses with and regulates genes related to the biogenesis, assembly, and remodeling of the cytoskeleton and cell wall. Our results suggest that SCL28 controls, not only cell proliferation as reported previously but also cell expansion and differentiation by promoting MCC exit and endoreplication and by modulating aspects of the biogenesis, assembly, and remodeling of the cytoskeleton and cell wall.

Experience of care through patients, family members and health professionals in an intensive care unit: a qualitative descriptive study.

BACKGROUND: A patient's stay in an intensive care unit (ICU) can lead to psychological and social alterations in patients and their nearest family circle. Healthcare staff and patients' family members strongly influence patients' stays in ICU. AIMS: To explore the perceptions about the experiences of patients in the ICU, their family members and nurses who attend them. METHODS: A descriptive phenomenological design was used to guide the research. A purposive sample of 27 male and female participated. Nine hospitalised patients, nine nurses and nine family members were interviewed in 2016 using a semi-structured, face to face interview. Steps of Giorgi phenomenological method were used to analyse the qualitative data. FINDINGS: Among the three categorised groups, there is a common point that is the constant of humanization in care and it is very clear on the part of the patient, the patient's family and the nurses whom they attend. In addition, both in the group of patients and in the group of families allude to the fear and anguish that responds to patients, this problem does not appear in the speeches of nurses, who are torn between excessive technology and the performance of their work correctly for better patient welfare. CONCLUSIONS: A stay in an ICU implies emotional expense for both patients and their families, which leads to a series of changes in their affective and social sphere, manifested by a set of requirements that must be responded by the multidisciplinary team that attends to patients. It would be fundamental to inform the health authorities about these requirements in order to modify the usual conduct habits displayed in ICU, which would help improve all levels for patients, their families and nurses.

Experiencia en el despliegue del piloto de galen clínicas en los laboratorios del instituto de hematología e inmunología / Experience in deploying of pilot galen clínicas laboratories in the institute of hematology and immunology

SOFTEL, es la empresa encargada por el Ministerio de las Comunicaciones de informatizar las entidades de Salud Pública. Para ello se han desarrollado varias aplicaciones, para ser desplegadas en hospitales, clínicas, policlínicos y bancos de sangre, fundamentalmente. Específicamente los laboratorios constituyen un área muy sensible debido a que en ellos se realizan todos los medios diagnósticos, que resultan de gran utilidad el almacenamiento de esta información para futuros estudios de los pacientes. Este trabajo expone la experiencia obtenida en el despliegue de dicho módulo, como una primera experiencia, conocida como despliegue de piloto o prueba beta. Esta experiencia sirve de base para las futuras instalaciones en otros clientes, reportó una serie de inconformidades que se resolvieron antes de concluir el mismo y finalmente quedo concluido el despliegue. Este tuvo complicaciones relacionadas con la existencia de laboratorios fuera del área de cobertura del Instituto y la preparación de las interfaces con los diferentes auto analizadores(AU)

SOFTEL is the enterprise commissioned by the Ministry of Communications to computerize public health institutions. For that has been developed several applications to be deployed in hospitals, clinics and blood banks, basically. Specifically, laboratories are a very sensitive area because in them all the diagnostic tools are used, so it is useful storage of this information for future studies of patients. This paper describes the experience gained in the deployment of the module, as a first experience, known as pilot or deployment of beta testing. This experience, which forms the basis for future installations on other customers, reported a series of disagrees which were resolved before the end of the same and finally the deployment was completed. This had complications related to the existence of laboratories outside the coverage area of the Institute and the preparation of the interfaces with the different analyzers(AU)

Experiencia en el despliegue del piloto de galen clínicas en los laboratorios del instituto de hematología e inmunología / Experience in deploying of pilot galen clínicas laboratories in the institute of hematology and immunology

SOFTEL, es la empresa encargada por el Ministerio de las Comunicaciones de informatizar las entidades de Salud Pública. Para ello se han desarrollado varias aplicaciones, para ser desplegadas en hospitales, clínicas, policlínicos y bancos de sangre, fundamentalmente. Específicamente los laboratorios constituyen un área muy sensible debido a que en ellos se realizan todos los medios diagnósticos, que resultan de gran utilidad el almacenamiento de esta información para futuros estudios de los pacientes. Este trabajo expone la experiencia obtenida en el despliegue de dicho módulo, como una primera experiencia, conocida como despliegue de piloto o prueba beta. Esta experiencia sirve de base para las futuras instalaciones en otros clientes, reportó una serie de inconformidades que se resolvieron antes de concluir el mismo y finalmente quedo concluido el despliegue. Este tuvo complicaciones relacionadas con la existencia de laboratorios fuera del área de cobertura del Instituto y la preparación de las interfaces con los diferentes auto analizadores(AU)

SOFTEL is the enterprise commissioned by the Ministry of Communications to computerize public health institutions. For that has been developed several applications to be deployed in hospitals, clinics and blood banks, basically. Specifically, laboratories are a very sensitive area because in them all the diagnostic tools are used, so it is useful storage of this information for future studies of patients. This paper describes the experience gained in the deployment of the module, as a first experience, known as pilot or deployment of beta testing. This experience, which forms the basis for future installations on other customers, reported a series of disagrees which were resolved before the end of the same and finally the deployment was completed. This had complications related to the existence of laboratories outside the coverage area of the Institute and the preparation of the interfaces with the different analyzers(AU)