RESUMO
The concentration and the length distribution of asbestos bodies in exposed human lungs have been determined in autopsy material by scanning electron microscopy after two different methods for preparing the tissue samples. The results demonstrate that the number of bodies vary between the right and left lung, as well as between the different lobes of a lung. The length distribution between the different lungs was, on the average, more or less the same; however small differences occurred between the different lobes. It was further shown that drying the tissue pieces before the analyses affected both the number and the length of the bodies. Examples of different types of asbestos bodies are presented, and their possible biological activity is discussed.
Assuntos
Amianto/análise , Neoplasias Pulmonares/patologia , Pulmão/patologia , Mesotelioma/patologia , Amianto/efeitos adversos , Amiantos Anfibólicos/análise , Asbestos Serpentinas/análise , Humanos , Neoplasias Pulmonares/etiologia , Masculino , Mesotelioma/etiologia , Microscopia Eletrônica de Varredura , Fibras Minerais/efeitos adversos , Fibras Minerais/análise , Manejo de Espécimes/métodosRESUMO
Analyzing fibers with electron microscopic techniques involves several preparation steps, especially during the analysis of fibers in human tissue. The influence of these steps on the analytical result was studied in detail. Fiber number was unaffected by the mild sonication of fiber suspensions analyzed with scanning electron microscopy. Significant fiber losses appeared during the filtration of fiber suspensions through 0.8-micrometer pore-size Nuclepore membranes. Shrinkage of the tissue during dry ashing broke lung fibers and consequently increased the fiber concentration and affected the fiber length distribution. Dry ashing, however, removed more of the organic debris than wet ashing; thus specimens prepared with dry ashing were more suitable for scanning electron microscopic analysis. A fairy good correlation was demonstrated for the analysis of fibers with scanning and transmission electron microscopy.
Assuntos
Amianto , Asbesto Crocidolita , Filtração , Temperatura Alta , Humanos , Microscopia Eletrônica de Varredura , UltrassomRESUMO
Standard UICC crocidolite fibres and fibres extracted from occupationally exposed human lung tissue have been analysed by scanninng electron microscopy after different preparation procedures. A significant fibre loss has been shown due to the adhesion of fibres to the breakers. Fibres are also discarded during the washing and extraction of the lung tissue samples. The recovery was found to be 30 to 40% both for UICC standard specimens and for lung tissue samples.