Antioxidant properties of Ferulago angulata and its hepatoprotective effect against N-nitrosodimethylamine-induced oxidative stress in rats.

CONTEXT: Ferulago angulata (Schlecht.) Boiss. (Apiaceae) (FASB) is used to treat liver diseases and has been used both as food and therapeutics by many cultures for thousands of years because of the natural antioxidant compounds. OBJECTIVE: This study determines antioxidant properties of FASB flowers, the levels of minerals and vitamins, and also, evaluates the hepatoprotective effect of flowers against N-nitrosodimethylamine (NDMA) induced on liver tissue by assessing antioxidant enzymes and histopathological parameters in Wistar albino rats. MATERIALS AND METHODS: In the study, the rats were divided into six groups of ten. Control, untreated animals were given 0.9% NaCl. Rats were intraperitoneally given NDMA (10 mg/kg) for the first 7 days. FASB methanol extract (150 and 300 mg/kg) was administered orally for 21 days. RESULTS: α-Tocopherol, retinol, ascorbic acid, total antioxidant activity, phenolic and flavonoid contents of FASB were 0.70 ± 0.13, 0.29 ± 0.03 µg/g, 139.32 ± 7.06 µg/100 g, 171.61 ± 6.05 mM ascorbic acid/g, 90.47 ± 4.11 mg GA/g and 37.39 ± 2.85 mg QE/g. DPPH and hydroxyl radical scavenging activity was obtained IC50 67.34 ± 4.14 and 64.87 ± 4.68 µg/mL, respectively. DISCUSSION AND CONCLUSION: The results of the study indicated that FASB flowers contain high levels of vitamins, minerals, total antioxidant activity, phenolics and flavonoids. Due to the positive effect on significant changes in antioxidant enzymes of liver tissue and histopathological examination, it is thought that the plant could be used as a hepatoprotective.

Chemical Composition and Antioxidant Activity of Two Wild Edible Mushrooms from Turkey.

The purpose of this study was to determine the antioxidant and antiradical activities in methanol extracts of two edible mushrooms from Turkey, Agaricus urinascens (Jul. Schäff. & F.H. Møller) Singer and Volvopluteus gloiocephala (DC.) Vizzini, Contu & Justo, as well as to examine trace elements (Cu, Zn, Fe, Mn, Co, Cr, Cd, Pb), minerals (Mg, Ca, K, Na) and vitamin C content. The levels of trace elements and minerals were determined by inductively coupled plasma atomic emission spectroscopy. Vitamin C and antioxidant properties were measured with a spectrophotometer. According to the results, total antioxidant activity and phenolic and flavonoid contents of A. urinascens and V. gloiocephala were 67.53 ± 2.54 mM ascorbic acid/g and 31.68 ± 2.27 mM ascorbic acid/g, 6.88 ± 0.31 mg GA/g and 5.22 ± 0.53 mg GA/g, and 3.80 ± 0.04 mg QE/g and 2.60 ± 0.20 mg QE/g, respectively. The findings of this study indicated that methanol extract of A. urinascens has a significant antioxidant activity and contents of total phenolic and flavonoid compounds. However, V. gloiocephala contains high levels of Ca, Cr, Fe, K, Mg, Mn, and Na, whereas A. urinascens contains higher levels of Cd, Co, Cu, Pb, and Zn than that of V. gloiocephala. Some trace elements that are in the compound of macrofungi may contribute the antioxidant capacity of macrofungi. A. urinascens has the ability to accumulate some heavy metals that prevent environmental metal pollution and can be used as a biomonitor. A. urinascens is expected to be used as an antioxidant in future studies of experimental animal models, against free radicals generated in response to oxidative stress.