RESUMO
For extraction of soluble antigen from cells infected with Epstein-Barr virus, Herpesvirus salmirl, and H. ateles, 0.1 M glycine (pH 9.5) was used. This method yielded increased amounts of the antigen containing much less cell debris. Lymphoblastoid cells infected with Epstein-Barr virus could maintain up to 50% viability after the extraction procedure. These cells could be used again after an appropriate interval in culture. The usefulness of this technique is discussed.
Assuntos
Antígenos Virais/análise , Glicina , Herpesviridae/imunologia , Herpesvirus Saimiriíneo 2/imunologia , Herpesvirus Humano 4/imunologia , Técnicas Imunológicas , Técnicas MicrobiológicasRESUMO
The in vitro sensitivity of oncogenic herpesviruses, Epstein-Barr virus (EBV), and Herpesvirus saimiri (HVS) to human interferon produced by normal human leukocytes (Le), lymphoblastoid cell lines (LYI), and diploid fibroblasts (Fi) was studied. Four virus strains were used: HVS S295C, the highly oncogenic HVS S396-O, the transforming B95-8 strain of EBV, and the nontransforming P3HR1 strain of EBV. All interferons were active when applied to the cells after absorption of HVS and P3HR1-EBV, although different amounts were required to achieve 50% inhibition of HVS-induced cytopathic effect or EBV-induced early antigen (EA) expression. Transformation of human umbilical cord blood lymphocytes (HCBL) by the B95-8 strain of EBV was prevented only by Le and LYI. In these experiments, the most effective inhibitor of the oncogenic herpesviruses was Le, and the least effective was Fi. The effect of polynucleotides poly(I).poly(C) and the complex of poly(I).poly(C) with poly-L-lysine and carboxymethylcellulose on HVS and EBV was also studied. Their inhibitory action was proportionate to the ability of herpesvirus-infected cells to produce interferon. Thus owl monkey kidney cells, which produce relatively high levels of interferon, required nanogram quantities of polynucleotides to become resistant to HVS. Transformation of HCBL by B95-8-EBV was also prevented by poly(I).poly(C). In Raji cells superinfected with P3HR1-EBV, polynucleotides failed to stimulate interferon, and higher EBV-induced EA expression was observed. The percentage of P3HR1 and Raji cells spontaneously expressing EBV-associated antigens remained unchanged after exposure to either interferon or polynucleotides.
Assuntos
Herpesvirus Saimiriíneo 2/efeitos dos fármacos , Herpesvirus Humano 4/efeitos dos fármacos , Indutores de Interferon/farmacologia , Interferons/farmacologia , Animais , Aotus trivirgatus , Linhagem Celular , Transformação Celular Viral , Genes Virais/efeitos dos fármacos , Herpesvirus Humano 4/genética , Rim , Linfócitos/efeitos dos fármacos , Polinucleotídeos/farmacologiaRESUMO
A micro-quantitative complement fixation (CF) procedure based on 51Cr release is described. The method employs 50% hemolysis as end point and the alternation equation to calculate the amount of complement involved in the hemolytic reaction. Compared to the conventional CF tests, the radiometric procedure described here is very precise and consistently reproducible. Also, since only 3 4-fold dilutions of sera are used for the titration of antibodies over a wide range of concentrations, the test is very concise and is economical to perform. Its format is amenable to automation and computerization. This radiometric CF procedure is thus most useful for large-scale immunological research and epidemiological surveillance studies.
Assuntos
Anticorpos , Testes de Fixação de Complemento/métodos , Animais , Autoanálise , Radioisótopos de Cromo , Proteínas do Sistema Complemento/imunologia , Computadores , Cobaias , Proteínas Hemolisinas , Humanos , Matemática , Radiometria , OvinosRESUMO
Four young strain III/J rabbits of both sexes were inoculated with a single dose of prototype partially purified Herpesvirus saimiri (HVS) via IV and IM routes. All inoculated animals had enlarged lymph nodes, and significant levels of antibodies to HVS early, late, and membrane antigens were detectable during the infection. The animals died or were killed and HVS was isolated from the peripheral blood mononuclear cells and the various lymph nodes but not from the kidney. Microscopic examination showed that these animals had poorly differentiated lymphomas. The response of mononuclear cells to PHA from peripheral blood of infected animals showed depressed cell mediated immune responses. Humoral and cellular immunity responses during tumorigenesis were comparable to those reported in nonhuman primates with HVS-induced tumors. Thus, the inbred strain III/J appears to be an inexpensive suitable model for studies of oncogenic herpesvirus-induced cancers.
Assuntos
Infecções por Herpesviridae/imunologia , Linfoma/imunologia , Infecções Tumorais por Vírus/imunologia , Animais , Feminino , Infecções por Herpesviridae/patologia , Herpesvirus Saimiriíneo 2/imunologia , Imunidade Celular , Linfoma/etiologia , Linfoma/patologia , Masculino , Neoplasias Experimentais/imunologia , Neoplasias Experimentais/patologia , Coelhos , Fatores de Tempo , Infecções Tumorais por Vírus/patologiaRESUMO
Humoral and cell-mediated immunity (CMI) to herpesvirus saimiri (HVS), an oncogenic lymphotropic herpesvirus, was studied in squirrel and rhesus monkeys. Natural antibody to HVS was found in five of six squirrel monkeys but there was no evidence of specific CMI directed against HVS. Rhesus monkeys did not show natural antibody or CMI against HVS antigens. Immunization with HVS, however, produced both antibody and specific CMI in the rhesus monkeys, but no CMI developed in the squirrel monkeys. These findings are important in the development of animal models for the treatment of tumors associated with lymphotropic herpesviruses.
Assuntos
Cebidae/imunologia , Herpesvirus Saimiriíneo 2/imunologia , Macaca mulatta/imunologia , Macaca/imunologia , Saimiri/imunologia , Animais , Formação de Anticorpos , Feminino , Infecções por Herpesviridae/imunologia , Imunidade Celular , Imunização , Masculino , Especificidade da Espécie , Infecções Tumorais por Vírus/imunologiaAssuntos
Carboximetilcelulose Sódica/farmacologia , Células-Tronco Hematopoéticas/efeitos dos fármacos , Indutores de Interferon/farmacologia , Metilcelulose/análogos & derivados , Peptídeos/farmacologia , Poli I-C/farmacologia , Polilisina/farmacologia , Irradiação Corporal Total , Animais , Relação Dose-Resposta à Radiação , Células-Tronco Hematopoéticas/efeitos da radiação , Macaca mulatta , Fatores de TempoAssuntos
Linfoma de Burkitt/epidemiologia , Testes de Fixação de Complemento , Herpesvirus Humano 4/imunologia , Neoplasias Nasofaríngeas/epidemiologia , Radiometria , Anticorpos Antivirais/biossíntese , Dinamarca , Feminino , Gana , Doença de Hodgkin/epidemiologia , Humanos , Mononucleose Infecciosa/epidemiologia , Masculino , Estudos Prospectivos , Singapura , Estados UnidosRESUMO
The origin of defective DNA (dDNA) of the Patton strain of herpes simplex virus type 1 (HSV-1) was physically mapped with BamHI in the parental DNA. The dDNA obtained from virus passaged at high multiplicities of infection was resistant to cleavage with HindIII, whereas digestion with EcoRI yielded a cluster of fragments 5.4 to 5.7 megadaltons (Mdal) in size. Cleavage with BamHI gave a cluster of fragments 2.6 to 3.2 Mdal in size, plus two homogeneous, comigrating 1-Mdal fragments. One of the latter fragments contained the single EcoRI site approximately 65 base pairs from one end. Hybridization of in vitro labeled dDNA probe to EcoRI, HindIII, BamHI, and Hpa I digests of nondefective HSV-1 DNA demonstrated that, in addition to the S-region terminal repeat, only one end of the S region was involved in the generation of this class of dDNA. Thus, the dDNA probe did not hybridize to either the S region 3.0-Mdal HindIIIN fragment or a 3.0-Mdal BamHI fragment of the adjacent 8.7-Mdal HindIIIG fragment, but did hybridize to four BamHI fragments of HindIII G (approximately 5.7 Mdal). The cluster of 2.6- to 3.2-Mdal fragments obtained with BamHI digestion of dDNA appears to represent a novel junction between the termination of dDNA adjacent to the 3.0-Mdal BamHI fragment in HindIII G and the 2.0- to 2.3-Mdal BamHI fragment terminal in HSV-1 DNA.
Assuntos
DNA Viral/análise , Simplexvirus/análise , Linhagem Celular , Enzimas de Restrição do DNA/metabolismo , Conformação de Ácido NucleicoRESUMO
12-0 tetradecanoyl phorbol 13-acetate (TPA), known to promote tumors in mice and also to enhance viral transformation as well as induction of viral antigens, was demonstrated to be mitogenic to peripheral blood mononuclear cells from rhesus monkeys and three species of marmosets. Even though mitogenic responses varied between species and within species, the mitogenic dose response due to TPA was comparable to the response of phytohemagglutinin (PHA-P). A significant synergistic effect of PHA-P and TPA on mononuclear cells from marmosets was evident when they were used together at optimal doses. TPA also increased the efficiency of in vitro transformation of marmoset lymphocytes by Epstein-Barr virus.
Assuntos
Transformação Celular Viral/efeitos dos fármacos , Herpesvirus Humano 4/fisiologia , Ativação Linfocitária/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Forbóis/farmacologia , Acetato de Tetradecanoilforbol/farmacologia , Animais , Callitrichinae , Células Cultivadas , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Haplorrinos , Linfócitos/microbiologia , Macaca mulatta , Fito-Hemaglutininas/farmacologiaRESUMO
Since patients with nasopharyngeal carcinoma were first reported to have elevated levels of IgA antibody to Epstein-Barr virus (EBV) in their sera, workers in a number of countries have studied the possibility that this assay could be used in the diagnosis and monitoring of patients with this disease. In the United States, a collaborative project involving seven centers has been established to investigate the potential value of IgA antibody to EBV viral capsid antigen (VCA) as a clinical tool. In this report, we will summarize the results obtained from three studies: a comparison of EBV serology in three laboratories; a retrospective study of 37 nasopharyngeal carcinoma (NPC) patients and controls, and a prospective study of 126 NPC patients and 683 controls, including 149 patients with other malignancies involving the head and neck. The study of testing comparability in three laboratories demonstrated the feasibility of using this assay in a number of laboratories. The retrospective study confirmed the difference in IgA antibody titers between NPC patients and matched controls. The prospective study showed a relationship between IgA antibody titers and histopathology but not disease stage. IgA antibody titers were elevated more frequently in patients with nonkeratinizing or poorly differentiated types of NPC than for the well-differentiated squamous cell carcinomas. While IgA antibodies to EBV VCA appear to be of value in the early detection and diagnosis of NPC, it is possible that additional serologic tests for immunity to EBV, such as IgG antibody to VCA or early antigen (EA), will improve even further the clinical value of EBV serology in the management of NPC.