Greenhouse gas emissions and soil properties following amendment with manure-derived biochars: Influence of pyrolysis temperature and feedstock type.

Manure-derived biochars can offer a potential option for the stabilization of manure, while mitigating climate change through carbon sequestration and the attenuation of nitrous oxide emission. A laboratory incubation study was conducted to assess the effects of four different manure-derived biochars produced from different feedstocks (poultry litter and swine manure) at different temperatures (400 or 600 °C). A commonly available standard wood chip biochar, produced at a greater temperature (1000 °C), and non-amended treatments were used as references. Two different soils (sandy and silt-loam) were amended with 2% (w/w) biochar on a dry soil weight basis (corresponding to 20 Mg ha(-1)), with the soil moisture being adjusted to 75% saturation level. After a pre-incubation period (21 days), 170 kg N ha(-1) of NH4NO3 fertilizer was added. Measurements of CO2, N2O, CH4 emissions and soil N mineralisation were carried out on different days during the 85 days of incubation. The net C mineralization and N2O emissions from both soils amended with poultry litter biochar at 400 °C were significantly greater than the other biochar treatments. Nitrate availability was greater in both soils in which the manure-derived biochar was used instead of the standard biochar. All of the biochars increased the pH of the silt-loam, sub-acid soil, but failed to improve the cation exchange capacities (CEC) in either soil. Total C and N, P, K and Mg (except Ca) were significantly increased in the manure-derived biochar amended soils, compared to the Control, and were positively correlated to the biochar nutrient contents. This study indicates that the soil application of biochar engenders effects that can vary considerably according to the biochar properties, as determined on the basis of the feedstock types and process conditions. Low-temperature biochar production from manure represents a possible way of producing a soil amendment that can stabilize C while supplying a significant quantity of nutrients.

Conversion from mineral fertilisation to MSW compost use: Nitrogen fertiliser value in continuous maize and test on crop rotation.

The recycling of agricultural wastes, co-products, and by-products is necessary for creating circular economic (closed loop) agro-food chains and more sustainable agro-ecosystems. The substitution of N mineral fertilisers with recycled organic fertiliser promotes a circular economy, makes the agricultural system more environmentally sustainable, and guarantees food security. Results from a continuous maize experiment and four-year rotation cropping systems (maize, winter wheat, maize, and soybean) were used in a three-year study that replaced part or all mineral fertilisers with Municipal Solid Waste Compost (MSWC). In the first experiment, two different fertilisation strategies, MSWC only (M-Com) and mineral fertilisers (M-Min), were compared with zero nutrients (M-Test 0), whereas in the rotation cropping systems, mineral fertilisation (R-Min) was compared with a combination of MSWC and mineral fertilisers (R-Com + Min). Depressed yields resulted in the initial year of compost application, but by the middle term (three years), MSWC fertilisation showed a good N fertiliser value, mainly for yield summer crops and integrated with N mineral fertilisers. Different soil indicators and the N content in crop tissues and soil suggested that the scarce N availability recorded mainly during the first year is responsible for yield reduction. Due to limited supplies of MSWC, soil total N and the stable organic fraction bound tightly to minerals (MOM), did not vary significantly in the three-year experiment. Conversely, the more labile organic fraction (fPOM) increased only in the top soil layers (0-15 cm). Also in the top layer, M-Com increased the amount of organic fraction occluded into soil aggregates (oPOM). Furthermore, replacement of N mineral fertiliser with compost effectively mitigated N2O emissions in wheat and maize. Overall, the fertiliser value of MSWC was maximised when it was used repeatedly and in combination with mineral fertiliser, especially in spring and summer crops.

Assessment of Methane and Nitrous Oxide Fluxes from Paddy Field by Means of Static Closed Chambers Maintaining Plants Within Headspace.

This protocol describes the measurement of greenhouse gas (GHG) emissions from paddy soils using the static closed chamber technique. This method is based on the diffusion theory. A known volume of air overlaying a defined soil area is enclosed within a parallelepiped cover (named "chamber"), for a defined period of time. During this enclosure period, gases (methane (CH4) and nitrous oxide (N2O)) move from soil pore air near their microbial source (i.e., methanogens, nitrifiers, denitrifiers) to the chamber headspace, following a natural concentration gradient. Fluxes are then estimated from chamber headspace concentration variations sampled at regular intervals throughout the enclosure and then analyzed with gas chromatography. Among the techniques available for GHG measurement, the static closed chamber method is suitable for plot experiments, as it does not require large homogenously treated soil areas. Furthermore, it is manageable with limited resources and can identify relationships among ecosystem properties, processes, and fluxes, especially when combined with GHG driving force measurements. Nevertheless, with respect to the micrometeorological method, it causes a minimal but still unavoidable soil disturbance, and allows a minor temporal resolution. Several phases are key to the method implementation: i) chamber design and deployment, ii) sample handling and analyses, and iii) flux estimation. Technique implementation success in paddy fields demands adjustments for field flooding during much of the cropping cycle, and for rice plant maintenance within the chamber headspace during measurements. Therefore, the additional elements to be considered with respect to the usual application of non-flooded agricultural soils consist of devices for: i) avoiding any unintended water disturbance that could overestimate fluxes, and ii) including rice plants within the chamber headspace to fully consider gases emitted through aerenchyma transportation.

Towards the co-ordination of terrestrial ecosystem protocols across European research infrastructures.

The study of ecosystem processes over multiple scales of space and time is often best achieved using comparable data from multiple sites. Yet, long-term ecological observatories have often developed their own data collection protocols. Here, we address this problem by proposing a set of ecological protocols suitable for widespread adoption by the ecological community. Scientists from the European ecological research community prioritized terrestrial ecosystem parameters that could benefit from a more consistent approach to data collection within the resources available at most long-term ecological observatories. Parameters for which standard methods are in widespread use, or for which methods are evolving rapidly, were not selected. Protocols were developed by domain experts, building on existing methods where possible, and refined through a process of field testing and training. They address above-ground plant biomass; decomposition; land use and management; leaf area index; soil mesofaunal diversity; soil C and N stocks, and greenhouse gas emissions from soils. These complement existing methods to provide a complete assessment of ecological integrity. These protocols offer integrated approaches to ecological data collection that are low cost and are starting to be used across the European Long Term Ecological Research community.