Gender hierarchy in the space: the role of gender status in shaping the spatial agency bias.

According to the Spatial Agency Bias (SAB), more agentic groups (men) are envisioned to the left of less agentic groups (women). This research investigated the role of social status in shaping the spatial representation of gender couples. Participants were presented pairs consisting of one male and one female target who confirmed gender stereotypes. The status of the targets in each pair was systematically varied (high-status vs. low-status job). Participants chose the target order (female/male vs. male/female) they preferred. In line with gender-status expectations (male: high-status, female: low-status), a male in a high-status job led to a spatial arrangement that favored the male/female order, regardless of the status of the female target. The female/male order was favored only when the female had a high-status job and the male a low-status job. No SAB occurred for pairs in which both targets displayed low-status jobs. The implications of status for the SAB are discussed.

Does Digital Intergroup Contact Reduce Prejudice? A Meta-Analysis.

With the ubiquity of technology in the modern life, contact between different social groups via different digital means is an everyday reality. Can such a digital intergroup contact reduce prejudice, or does it exacerbate it? In this study, we summarize the present evidence on the links between intergroup digital contact and prejudice in a variety of intergroup contexts. We meta-analyzed experimental and correlational evidence from 88 independent samples (n = 9,385). Digital intergroup contact showed a small but significant effect consistent with prejudice reduction (g = 0.25). Direct digital contact showed larger effect than indirect forms of contact, and contact via computer-mediated communication showed larger effects than contact achieved via other activities such as interacting with nonplayable characters or embodiment. The effects were similar regardless of the type of out-group targeted by prejudice. Overall, meta-analytical results suggest that digital intergroup contact may reduce prejudice.

An Intersectional Perspective on Age Stereotypes: Bisexual and Gay Men are Stereotyped as Younger than Heterosexual Men Even When Aging.

We analyzed the age stereotypes of heterosexual, gay, and bisexual men and the implications of such stereotypes for the conceptualization of older gay and bisexual men, specifically. In Study 1a (N = 158) and 1b (N = 155), we found that compared to heterosexual men, participants stereotyped gay men more on young- than elderly-stereotypical traits. Participants represented bisexual men not as "somewhere in between" the stereotypes about heterosexual and gay men, but were characterized more by young- than elderly-stereotypical traits. In Study 2 (N = 106), we reasoned that because of their sexual orientation, both older gay and bisexual men would be viewed as atypical subtypes of older men, considered to be heterosexual by default. As atypical subtypes, both older gay and bisexual men may be stereotyped less on traits associated with elderly men and more on traits associated with their sexual orientation membership, namely young-stereotypical traits. Consistently, compared with older heterosexual men, both older gay and bisexual men were perceived as less typical of older men, and their perceived atypicality accounted for them being stereotyped less as older and more as younger men. The results have been examined for intersectional stereotyping research and practical implications are discussed.

Facing Europe: visualizing spontaneous in-group projection.

Individuals perceive their own group to be more typical of a shared superordinate identity than other groups are. This in-group projection process has been demonstrated with both self-report and indirect measures. The two studies reported here extend this research to the visual level, specifically, within the domain of faces. Using an innovative reverse-correlation approach, we found that German and Portuguese participants' visual representations of European faces resembled the appearance typical for their own national identity. This effect was found even among participants who explicitly denied that one nation was more typical of Europe than the other (Study 1). Moreover, Study 2 provides experimental evidence that in-group projection is restricted to inclusive superordinate groups, as the effect was not observed for visual representations of a category ("Australian") that did not include participants' in-group. Implications for the in-group projection model, as well as for the applicability of reverse-correlation paradigms, are discussed.

Trust predicts COVID-19 prescribed and discretionary behavioral intentions in 23 countries.

The worldwide spread of a new coronavirus (SARS-CoV-2) since December 2019 has posed a severe threat to individuals' well-being. While the world at large is waiting that the released vaccines immunize most citizens, public health experts suggest that, in the meantime, it is only through behavior change that the spread of COVID-19 can be controlled. Importantly, the required behaviors are aimed not only at safeguarding one's own health. Instead, individuals are asked to adapt their behaviors to protect the community at large. This raises the question of which social concerns and moral principles make people willing to do so. We considered in 23 countries (N = 6948) individuals' willingness to engage in prescribed and discretionary behaviors, as well as country-level and individual-level factors that might drive such behavioral intentions. Results from multilevel multiple regressions, with country as the nesting variable, showed that publicized number of infections were not significantly related to individual intentions to comply with the prescribed measures and intentions to engage in discretionary prosocial behaviors. Instead, psychological differences in terms of trust in government, citizens, and in particular toward science predicted individuals' behavioral intentions across countries. The more people endorsed moral principles of fairness and care (vs. loyalty and authority), the more they were inclined to report trust in science, which, in turn, statistically predicted prescribed and discretionary behavioral intentions. Results have implications for the type of intervention and public communication strategies that should be most effective to induce the behavioral changes that are needed to control the COVID-19 outbreak.

Non-invasive assessment of coronary flow reserve and ADMA levels: a case-control study of early rheumatoid arthritis patients.

OBJECTIVE: Plasma concentration of asymmetric dimethylarginine (ADMA), a major endogenous inhibitor of nitric oxide synthase, is considered a novel risk factor for endothelial dysfunction associated with enhanced atherosclerosis. Coronary microcirculation abnormalities have been demonstrated in patients with early rheumatoid arthritis (ERA) without any signs or symptoms of coronary artery disease (CAD). The aim of the study was to compare the ERA and control groups with ADMA, intima-media thickness (IMT) and coronary flow reserve (CFR) levels. It assessed whether ERA patients have more cardiovascular risk (endothelial dysfunction and coronary microvascular abnormalities), and evaluated whether any difference in IMT/CFR between ERA and controls can be explained by any difference in ADMA levels between the groups. METHODS: The study involved 25 ERA patients (female/male 21/4; mean age 52.04 +/- 14.05 years; disease duration

Asymmetric dimethylarginine (ADMA): an endogenous inhibitor of nitric oxide synthase and a novel cardiovascular risk molecule.

Asymmetric dimethylarginine (ADMA), a methyl derivate of the amino acid arginine, is produced by the physiological degradation of methylated proteins. ADMA is the major endogenous inhibitor of nitric oxide synthase (NOS), the enzyme which synthesizes nitric oxide (NO), a molecule endowed with important anti-atherosclerotic properties. Increased plasma ADMA concentrations cause impaired NO synthesis leading to endothelial dysfunction and atherosclerotic vascular disease. Increased plasma ADMA levels mainly occur following inhibition of the enzyme responsible for ADMA catabolism, dimethylarginine dimethylaminohydrolase (DDAH), by oxidative stress triggered by several cardiovascular risk factors. This paper reviews the effects on cardiovascular function produced by ADMA administration to experimental animals and humans. In addition, a number of clinical conditions associated with increased plasma ADMA concentrations are considered. Then the growing body of literature indicating that plasma ADMA levels have a predictive value for major cardiovascular events in prospective studies is discussed. Finally, an analysis is provided of the published data concerning the possibility to modulate plasma ADMA levels using drugs belonging to different pharmacological classes.

Nomina sunt omina: on the inductive potential of nouns and adjectives in person perception.

Six studies (N = 491) investigated the inductive potential of nouns versus adjectives in person perception. In the first 5 studies, targets were either described by an adjective (e.g., Mark is homosexual) or by the corresponding noun (e.g., Mark is a homosexual) or by both (Study 3). The authors predicted and found that nouns, more so than adjectives, (a) facilitate descriptor-congruent inferences but inhibit incongruent inferences (Studies 1-3), (b) inhibit alternative classifications (Study 4), and (c) imply essentialism of congruent but not of incongruent preferences (Study 5). This was supported for different group memberships and inclinations (athletics, arts, religion, sexual preference, drinking behavior, etc.), languages (Italian and German), and response formats, suggesting that despite the surface similarity of nouns and adjectives, nouns have a more powerful impact on person perception. Study 6 investigated the inverse relationship, showing that more essentialist beliefs (in terms of a genetic predisposition rather than training) lead speakers to use more nouns and fewer adjectives. Possible extensions of G. R. Semin and K. Fiedler's (1988) linguistic category model and potential applications for language use in intergroup contexts are discussed.

Imagined Intergroup Physical Contact Improves Attitudes Toward Immigrants.

In this set of research, we investigated the effects of intergroup physical contact on intergroup attitudes by relying on indirect contact strategies, namely the imagined contact paradigm. We implemented the imagined contact paradigm by leading participants to shape the mental imagery upon pictorial information. Specifically, in Study 1 participants saw a picture of a white hand touching a black hand [i.e., intergroup physical contact condition (InterPC)] or a picture of an outdoor scene (i.e., control condition), and were asked to imagine being either the toucher or in the outdoor scene, respectively. Results demonstrated that InterPC compared to control condition reduced intergroup bias. In Study 2 we compared the InterPC condition to a condition in which participants saw a white hand touching another white hand [i.e., intragroup physical contact (IntraPC)], and imagined to be the toucher. Again, we found that participants in the InterPC condition showed reduced intergroup bias compared to the IntraPC. Study 3 replicated results of Studies 1 and 2 by using an implicit measure of prejudice. Also, Study 3 further showed that asking participants to merely look at the picture of a white hand touching a black hand, without imagining being the toucher was not effective in reducing implicit prejudice. Results were discussed with respect to the literature on physical contact and prejudice reduction processes.

Asymmetric dimethylarginine (ADMA) induces vascular endothelium impairment and aggravates post-ischemic ventricular dysfunction in rats.

Asymmetric dimethylarginine (ADMA) is an endogenous nitric oxide (NO) inhibitor recognized as an independent risk factor for endothelial dysfunction and coronary heart diseases. This study investigated whether ADMA (10 mg/kg day for 14 days) affected endothelial function and aggravated post-ischemic ventricular dysfunction in the perfused rat heart. Systolic blood pressure and heart rate, plasma levels of ADMA and nitrite/nitrate were measured in vehicle- and ADMA-treated rats. Perfused hearts were submitted to global ischemia-reperfusion and vascular endothelial dysfunction was examined with angiotensin II in coronary vessels and aortic rings. Endothelial NO synthase (eNOS) and angiotensin-converting enzyme (ACE) mRNA expression in aortic and cardiac tissues were measured. ADMA-treated rats had higher systolic blood pressure (1.3-fold, P<0.01) and slower heart rate (16%, P<0.05) than controls. Plasma ADMA rose (1.9-fold, P<0.01) and nitrite/nitrate concentration decreased 59% (P<0.001). Ventricular contraction (stiffness) increased significantly, with worsening of post-ischemic ventricular dysfunction. In preparations from ADMA-treated rats the coronary vasculature's response to angiotensin II was almost doubled (P<0.01) and the maximal vasorelaxant effect of acetylcholine in aortic rings was significantly lower than in preparations from vehicle-treated rats. In cardiac and aortic tissues eNOS mRNA and ACE mRNA levels were similar in controls and ADMA-treated rats. The increased plasma levels of ADMA presumably cause endothelial dysfunction because of a deficiency in NO production, which also appears involved in the aggravation of myocardial ischemia-reperfusion injury.

Increased tumor necrosis factor-alpha and prostaglandin E2 concentrations in the cerebrospinal fluid of rats with inflammatory hyperalgesia: the effects of analgesic drugs.

BACKGROUND: We examined the changes in cerebrospinal fluid (CSF) concentrations of prostaglandin E2 (PGE2) and tumor necrosis factor-alpha (TNF-alpha) after intraplantar administration of complete Freund's adjuvant (CFA) in rats. In addition, we investigated whether different analgesic drugs orally administered at antihyperalgesic doses were able to prevent the changes in PGE2 and TNF-alpha spinal levels associated with hindpaw inflammation. METHODS: The Randall-Selitto paw-withdrawal test was used to measure inflammatory hyperalgesia. Tramadol (7.5 mg/kg), paracetamol (65 mg/kg), tramadol plus paracetamol and nimesulide (5 mg/kg) were administered orally twice a day, starting from the first day after the CFA injection. PGE2 in the CSF was measured by enzyme immunoassay, and TNF-alpha by ELISA. Behavioral and biochemical parameters were measured on Day 7 after intraplantar injection of CFA or saline. RESULTS: Withdrawal thresholds to mechanical stimuli decreased markedly in the CFA-treated paw. In these animals the quantification of proinflammatory mediators in the CSF revealed a significant increase in both PGE2 and TNF-alpha concentrations. All the pharmacological treatments prevented the development of the hyperalgesia as well as the PGE2 increase in the CSF. Conversely, a prevention of the increase in TNF-alpha levels was observed only in rats treated with nimesulide or tramadol and paracetamol in combination. CONCLUSIONS: Our results demonstrate that peripheral inflammatory hyperalgesia is associated with significant changes of proinflammatory mediators in the CSF. It is important to note, however, that spinal PGE2 and TNF-alpha proved to be differently affected by pharmacological treatments able to fully abolish the hyperalgesia.

Automatic female dehumanization across the menstrual cycle.

In this study, we investigate whether hormonal shifts during the menstrual cycle contribute to the dehumanization of other women and men. Female participants with different levels of likelihood of conception (LoC) completed a semantic priming paradigm in a lexical decision task. When the word 'woman' was the prime, animal words were more accessible in high versus low LoC whereas human words were more inhibited in the high versus low LoC. When the word 'man' was used as the prime, no difference was found in terms of accessibility between high and low LoC for either animal or human words. These results show that the female dehumanization is automatically elicited by menstrual cycle-related processes and likely associated with an enhanced activation of mate-attraction goals.

Adolescents' Appraisal of Homophobic Epithets: The Role of Individual and Situational Factors.

Homophobic epithets have become commonly used insults among adolescents. However, evidence suggests that there are differences in how these homophobic epithets are evaluated based on beliefs held by the observer and the context in which they are used. To examine this, Italian high school students were asked to rate the offensiveness of homophobic epithets, as well as to consider how they or others would react to homophobic epithets across various situations. Homophobic beliefs and beliefs about the social acceptability of homophobic epithets were also examined. It was found that greater perceived social acceptability of homophobic epithets was related to dismissive reactions to their use, whereas homophobic beliefs were predictive of negative emotional reactions but in varying ways depending on the specific context. The results indicate that homophobic epithets may not always be perceived as homophobic by adolescents, and that attempts to alter the social acceptability of these insults may be an effective manner of reducing their use.

Angiotensin-converting enzyme inhibition and angiotensin AT1-receptor antagonism equally improve endothelial vasodilator function in L-NAME-induced hypertensive rats.

Male Sprague-Dawley rats given N(omega)-nitro-L-arginine methyl ester (L-NAME) in drinking water for 8 weeks showed: (1) a clear-cut increase in systolic blood pressure; (2) a consistent decrease of endothelial-cell nitric oxide synthase (eNOS) gene expression in aortic tissue; (3) a marked reduction of plasma nitrite/nitrate concentrations; (4) a reduction of the relaxant activity of acetylcholine (ACh, from 10(-10) to 10(-4) M) on norepinephrine-precontracted aortic rings (reduction by 48+/-5%); (5) a marked decrease (-58%) of the basal release of 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) from aortic rings. In L-NAME-treated rats, administration in the last 4 weeks of either the angiotensin-converting enzyme (ACE) inhibitor enalapril (10 mg/kg/day in tap water) or the angiotensin AT(1)-receptor antagonist losartan (10 mg/kg/day in tap water) decreased systolic blood pressure levels, completely restored eNOS mRNA levels in aortic tissue and plasma nitrite/nitrate levels, and allowed a consistent recovery of both the relaxant activity of acetylcholine and the generation of 6-keto-PGF1alpha. Coadministration of icatibant, a bradykinin B(2)-receptor antagonist (200 microg/kg/day), with enalapril blunted the stimulatory effect of the ACE inhibitor on eNOS mRNA expression, circulating levels of nitrite/nitrate, the relaxant activity of ACh and the release of 6-keto-PGF1alpha in L-NAME-treated rats. The generation of 6-keto-PGF1alpha from aortic rings was also decreased in rats coadministered icatibant with losartan. These findings indicate that (1) the ACE inhibitor enalapril and the angiotensin AT(1)-receptor blocker losartan are equally effective to reverse NAME-induced endothelial dysfunction; (2) the beneficial effect of enalapril on the endothelial vasodilator function in L-NAME-treated rats is mediated by bradykinin B(2)-receptor activation; and (3) the enhanced endothelial generation of prostacyclin induced by losartan in L-NAME rats is also mediated by bradykinin B(2)-receptor activation.

Diclofenac does not modify morphine bioavailability in cancer patients.

We determined morphine plasma concentrations in 6 cancer patients before and with administration of diclofenac for 5 days. The non-steroidal anti-inflammatory drug does not modify morphine bioavailability. This observation suggests that diclofenac can be used in association with morphine during cancer pain treatment, without increasing the risk of overdosage or side effects of the opiate.

The analgesic drug tramadol prevents the effect of surgery on natural killer cell activity and metastatic colonization in rats.

Surgery stress has been shown to be associated in rat with decreased natural killer (NK) cell activity and enhancement of tumor metastasis. We have previously shown that the analgesic drug tramadol stimulates NK activity both in the rodent and in the human. In the present study, we analyze, in the rat, tramadol ability to prevent the effect of experimental surgery on NK activity and on the enhancement of metastatic diffusion to the lung of the NK sensitive tumor model MADB106. The administration of tramadol (20 and 40 mg/kg) before and after laparatomy significantly blocked the enhancement of lung metastasis induced by surgery. In contrast, the administration of 10 mg/kg of morphine was not able to modify this enhancement. The modulation of NK activity seemed to play a central role in the effect of tramadol on MADB106 cells. In fact, both doses of tramadol were able to prevent surgery-induced NK activity suppression, while the drug significantly increased NK activity in normal non-operated animals. Morphine, that in normal rats significantly decreased NK cytotoxicity, did not prevent surgery-induced immunosuppression. The good analgesic efficacy of tramadol combined with its intrinsic immunostimulatory properties suggests that this analgesic drug can be particularly indicated in the control of peri-operative pain in cancer patients.

Cognitive function in young and adult IL (interleukin)-6 deficient mice.

Interleukin-6 (IL-6) is a cytokine shown to affect brain function and to be involved in pathological neurodegenerative disorders such as Alzheimer's disease (AD). In the present study we investigated the cognitive function in transgenic mice not expressing IL-6 (IL-6 KO) and in wild type (WT) genotype at 4 and 12 months of age, using a passive avoidance and an eight-arm radial maze tasks. Motor function was quantified using an Animex apparatus. Hippocampal choline acetyltransferase (ChAT) activity was evaluated in both genotypes. No difference was observed in both genotypes for spontaneous motor activity. The mean latency (s) to re-enter the shock box, was similar in both young mutant and WT mice. However, a decreased sensitivity (50%) to scopolamine (1 mg/kg) in mutant compared to WT mice, was obtained. IL-6 KO mice exhibited a facilitation of radial maze learning over 30 days, in terms of a lower number of working memory errors and a higher percentage of animals reaching the criterion as compared with WT genotype tested at both ages. Furthermore, mutant mice, at the age of 12 months, showed a faster acquisition (22 days versus 30 days to reach the criterion). The pattern of arm entry exhibited by IL-6 KO mice showed a robust tendency to enter an adjacent arm at both ages, while WT only at the age of 4 months. ChAT activity was inversely correlated with memory performance. These findings suggest a possible involvement of IL-6 on memory processes, even if the mechanism remains still unclear.

Increased substance P and tumor necrosis factor-alpha level in the paws following formalin injection in rat tail.

We previously described a rat model where the injection of formalin in the tail induced a facilitation of the hindpaw withdrawal reflexes (hyperalgesia). In the present work, after injecting formalin in the tail, we measured the levels of pro-nociceptive mediators tumor necrosis factor-alpha (TNF) and substance P (SP) in the rat paws. A significant increase of SP levels was evident in the hindpaw, whereas no changes in SP were observed in the forepaw. Both in the hindpaw and in the forepaw the TNF levels were higher than normal at each stage of measurement. Our results indicate that a prolonged neuronal activation induced by formalin injection is associated with a change in nociceptive and inflammatory mediators in distal sites of the body. The fact that SP levels are changed in the hindpaw but not in the forepaw might point to the activation of a mechanism of retrograde signaling from central synapses to paw afferent nerves.

The use of methadone for cancer pain.

Methadone is not a new analgesic drug [69]. Several studies have demonstrated that methadone is a valid alternative to morphine, hydromorphone, and fentanyl for the treatment of cancer-related pain, and extensive reviews on the subject have been published in recent years [10,23,25,64,70,71]. Most people involved in pain therapy, however, are not well informed about the properties of methadone. The authors believe that the low cost of methadone paradoxically contributes to the limited knowledge of its characteristics and to the restricted therapeutic use of this drug. The low cost of methadone means there is little financial incentive for pharmaceutical companies to invest in research or to disseminate scientific information. Unfortunately, the lack of scientific information from pharmaceutical companies frequently results in a lack of knowledge on the part of physicians. Unless the existing approach changes, both culturally and politically, ignorance about methadone will persist among medical experts. The low cost of methadone, rather than being an advantage, will result in the limited exploitation of an effective drug.

Acute and carryover effects in mice of MDMA ("ecstasy") administration during periadolescence.

In spite of the increasing evidence concerning its neurotoxicity, young human individuals are often involved in the recreational use of amphetamine-type stimulants such as 3,4-methylenedioxymethamphetamine (MDMA or "ecstasy"). A study aimed to investigate short- and long-term consequences of a repeated and intermittent MDMA administration (0, 5 or 10 mg/kg i.p., 3 days treatment history) was conducted in mice. Mice were injected at different phases in development, namely at early (28 days old), middle (38 days old) or late (52 days old) adolescence. When assessed for nociceptive response, a dose-dependent analgesia was found in middle and late adolescent mice. Carryover consequences of previous MDMA treatment were then investigated at adulthood (80 days old). In a social interaction test, levels of environment exploration and social behaviour resulted markedly increased in drug-free state as a function of drug exposure during development, whereas others behaviours were reduced. MDMA challenge (5-mg/kg dose) produced the expected hyperactivity, as well as a marked increment of hypothalamic serotonin (5-hydroxyhyptamine, 5-HT) levels. Mice treated chronically with MDMA during middle and late adolescence were associated with important reductions of the indoleamine. As a whole, these results indicate a differential long-term vulnerability to behavioural and neurotoxicant effects of MDMA as a function of the developmental stage of exposure.