RESUMO
A combined morphological and biochemical investigation of mitochondria from developing and rapidly growing tissues ( tumors, fetal, and very early neonatal rat liver) revealed mitochondria which were deficient in respiratory control, showed no valinomycin induced K(+) accumulation or spontaneous Ca(++) uptake, and were unable to undergo a swelling-contraction cycle. Electron microscopic examination of fetal and neonatal livers and a mammary tumor revealed mitochondria which differed from controls with respect to matrix density and ability to undergo reversible structural changes. The importance of isolation and assay media in interpretation of results is emphasized.
Assuntos
Transporte Biológico Ativo/efeitos dos fármacos , Fígado/citologia , Neoplasias Mamárias Experimentais/patologia , Mitocôndrias/metabolismo , Animais , Animais Recém-Nascidos , Antibacterianos/farmacologia , Cálcio/metabolismo , Feminino , Feto , Masculino , Microscopia Eletrônica , Consumo de Oxigênio , Potássio/metabolismo , Gravidez , RatosRESUMO
Viral endocarditis developed in 24 percent of 50 newborn mice 6 to 8 days after intraperitoneal inoculation with murine adenovirus. Typical adenovirus intranuclear inclusions were seen in heart-valve lesions, and high titers of virus were recovered from heart tissue. Furthermore, adenovirions were directly visualized by electron microscopy within endothelial cells and fibroblasts of the heart valves.