RESUMO
The infiltrating mononuclear cell (MNC) type, and the localization of major histocompatability class I and class II antigens within 1,2-dimethylhydrazine (CAS: 540-73-8)-induced colonic tumors and normal colonic mucosa in WF/Hsd BR rats were investigated by immunoperoxidase staining of frozen sections with the use of monoclonal antibodies. Infiltrating T-cells stained with monoclonal antibodies W3/13, W3/25, and MRC OX 8; W3/13+ cells were predominant. The most numerous and consistently observed infiltrating cell type was an la antigen-bearing (MRC OX 6+, MRC OX 17+) macrophage. A smaller subpopulation of macrophages, staining with W3/25, showed a similar distribution within tumors. In adenomas and in some well-differentiated adenocarcinomas, the infiltrating MNC were concentrated at the tumor periphery and were in close proximity to neoplastic epithelia, but without evidence of consequent tumor cell necrosis. In all other tumors, infiltrating host cells were confined to the connective tissue stroma dividing clusters of neoplastic glands. The extent of cellular infiltration and the phenotypes of infiltrating cells did not correlate with the degree of tumor differentiation or with tumor size. Expression of MHC class I antigens (MRC OX 18) by tumor cells did not differ from that of normal epithelia, and neoplastic epithelia were uniformly negative for class II antigens (MRC OX 6, MRC OX 17). The data do not support a role for cytotoxic macrophages or T-cells in the local response to colon tumors.
Assuntos
Neoplasias do Colo/patologia , Macrófagos/patologia , Linfócitos T/patologia , 1,2-Dimetilidrazina , Animais , Colo/citologia , Neoplasias do Colo/induzido quimicamente , Neoplasias do Colo/imunologia , Dimetilidrazinas , Antígenos de Histocompatibilidade Classe II/análise , Complexo Principal de Histocompatibilidade , Masculino , Ratos , Ratos EndogâmicosRESUMO
Total RNA was isolated from in vitro activated lamina propria lymphocytes and used to direct the synthesis of cDNA. Interleukin 4 transcripts were then specifically amplified by PCR. Comparison of the nucleotide sequence with its human homologue demonstrates deletion within the coding region of pig interleukin 4 centred around amino acid residue 70 in the mature human protein.
Assuntos
DNA/biossíntese , Interleucina-4/genética , Linfócitos/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Dados de Sequência Molecular , RNA Mensageiro/isolamento & purificação , Alinhamento de Sequência , Especificidade da Espécie , SuínosRESUMO
A method is described for the quantitative assay of cellular MHC class II proteins. The assay has been developed for special use with epithelial cells isolated from the intestine, but has been successfully used with other cell types. It comprises a competitive immunoassay of anti-class II activity in cell lysates, using IgG-coated Sephacryl S300 as the solid phase and is sensitive over the range 1 ng-1 microgram.
Assuntos
Antígenos de Histocompatibilidade Classe II/análise , Radioimunoensaio/métodos , Resinas Acrílicas , Animais , Anticorpos Monoclonais/imunologia , Ligação Competitiva , Membrana Celular/imunologia , Epitélio/imunologia , Feminino , Imunoglobulina G/imunologia , Indicadores e Reagentes , Intestinos/imunologia , Masculino , Camundongos , Ratos , Reprodutibilidade dos TestesRESUMO
A monoclonal antibody is described which recognises an epitope associated with a receptor for interleukin-2 (IL-2) on pig lymphocytes. The monoclonal antibody inhibits high affinity binding of radiolabelled recombinant human IL-2 (rhIL-2) by pig lymphoblasts and also non-competitively inhibits both pig-TCGF and rhIL-2 maintained proliferation. By flow cytometry the antigen is apparently not present on freshly isolated blood lymphocytes but is detectable on small cells between 6 and 12 h after activation and on large cells by 24-h. These findings are comparable with those obtained using monoclonal antibodies recognising the 55 kDa alpha chain of the human and mouse IL-2 receptor (p55, TAC) expressed on activated cells in vivo and in vitro. However, the molecular weight of the porcine antigen is between 65 and 70 kDa.
Assuntos
Anticorpos Monoclonais/imunologia , Epitopos/análise , Receptores de Interleucina-2/imunologia , Animais , Ligação Competitiva , Interleucina-2/farmacologia , Ativação Linfocitária , Peso Molecular , Receptores de Interleucina-2/análise , Proteínas Recombinantes/farmacologia , SuínosRESUMO
A panel of four monoclonal antibodies produced in our laboratory, MIL1, MIL2, MIL3, MIL4, and the type-specific monocyte/granulocyte marker 74-22-15 were used to isolate and to discriminate between monocytes, macrophages and granulocytes derived from porcine peripheral blood, lung and gut lamina propria. Two-colour flow cytometry and cell sorting showed that while no monoclonal antibody was specific for just a single cell population, each cell type had a unique and characteristic combination of surface antigens. These differences could be used to identify and purify monocytes, macrophages, neutrophils, eosinophils and basophils from the three different sites. The study also demonstrated similarities and differences within cell types from the same site and from different sites: polymorphonuclear neutrophils (PMN) from peripheral blood were subdivided into two subpopulations by the presence or absence of the surface antigen recognized by MIL4, while PMN from alveolar lavage did not express this antigen. Peripheral blood eosinophils were also divided into subpopulations by the presence or absence of the same surface antigen. Lamina propria eosinophils strongly expressed the MIL4 marker and differed morphologically from blood eosinophils. Peripheral blood basophils and lamina propria mast cells were morphologically similar and expressed similar antigens. Monocytes and alveolar macrophages also expressed the same surface antigens.
Assuntos
Anticorpos Monoclonais/imunologia , Granulócitos/imunologia , Macrófagos/imunologia , Monócitos/imunologia , Animais , Especificidade de Anticorpos , Antígenos de Superfície/imunologia , Líquido da Lavagem Broncoalveolar/citologia , Separação Celular , Citometria de Fluxo , Hibridomas , Imuno-Histoquímica , Mucosa Intestinal/citologia , Jejuno/citologia , Mastócitos/imunologia , Mucosa/citologia , SuínosRESUMO
Using a CD4+ T-cell-transplanted SCID mouse model of colitis, we have analyzed TGF-beta transcription and translation in advanced disease. By in situ hybridization, the epithelium of both control and inflamed tissues transcribed TGF-beta1 and TGF-beta3 mRNAs, but both were expressed significantly farther along the crypt axis in disease. Control lamina propria cells transcribed little TGF-beta1 or TGF-beta3 mRNA, but in inflamed tissues many cells expressed mRNA for both isoforms. No TGF-beta2 message was detected in either control or inflamed tissues. Immunohistochemistry for latent and active TGF-beta1 showed that all cells produced perinuclear latent TGF-beta1. The epithelial cell basal latent protein resulted in only low levels of subepithelial active protein, which co-localized with collagen IV and laminin in diseased and control tissue. Infiltrating cells expressed very low levels of active TGF-beta. By ELISA, very low levels (0-69 pg/mg) of soluble total or active TGF-beta were detected in hypotonic tissue lysates. TGF-beta1 and TGF-beta3 are produced by SCID mouse colon and transcription is increased in the colitis caused by transplantation of CD4+ T-cells, but this does not result in high levels of soluble active protein. Low levels of active TGF-beta may be a factor contributing to unresolved inflammation.
Assuntos
Colite/imunologia , Doenças Inflamatórias Intestinais/imunologia , Fator de Crescimento Transformador beta/biossíntese , Animais , Linfócitos T CD4-Positivos/transplante , Colite/etiologia , Colite/genética , Colite/patologia , Colo/patologia , Tecido Conjuntivo/patologia , Ensaio de Imunoadsorção Enzimática , Técnica Indireta de Fluorescência para Anticorpo , Imuno-Histoquímica , Hibridização In Situ , Doenças Inflamatórias Intestinais/etiologia , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/patologia , Camundongos , Camundongos SCID , RNA Mensageiro/isolamento & purificação , Distribuição Tecidual , Fator de Crescimento Transformador beta/genéticaRESUMO
Major histocompatibility complex (MHC) class II antigens were identified on cells within mammary gland connective tissue of lactating mice using a paraformaldehyde-lysine-periodate-gluteraldehyde fixative and an immunoperoxidase staining method. The distribution of class II expressing cells within interalveolar and interlobular connective tissue was similar both throughout lactation and in successive lactations. Epithelial cells within secretory alveoli and mammary ducts did not express class II antigens.
Assuntos
Antígenos de Histocompatibilidade Classe II/metabolismo , Glândulas Mamárias Animais/imunologia , Animais , Feminino , Técnicas Imunoenzimáticas , Intestino Delgado/imunologia , Lactação/imunologia , Glândulas Mamárias Animais/anatomia & histologia , Camundongos , Camundongos Endogâmicos BALB C , Baço/imunologia , Distribuição TecidualRESUMO
The ability of cells isolated from bovine milk and peripheral blood to present soluble protein and particulate bacterial antigens to peripheral blood T lymphocytes was compared using a culture system which consistently supports antigen-specific, primary, proliferative responses. The present study shows that cells from blood and from milk can present antigen to unprimed T cells. Major histocompatibility complex class II restriction of the responses was demonstrated by abrogation of proliferation by the addition of anti-bovine class II monoclonal antibody to cultures. Although cells derived from blood or milk were shown to be capable of presenting antigen to T cells, differences in optimal culture conditions and kinetics of the resulting response were observed.
Assuntos
Apresentação de Antígeno , Células Apresentadoras de Antígenos/imunologia , Antígenos/imunologia , Bovinos/imunologia , Leite/citologia , Linfócitos T/imunologia , Animais , Anticorpos Monoclonais , Antígenos de Bactérias/imunologia , Células Cultivadas , Feminino , Citometria de Fluxo/veterinária , Antígenos de Histocompatibilidade Classe II/imunologia , Leucócitos Mononucleares/imunologia , Ativação Linfocitária , Solubilidade , Streptococcus/imunologiaRESUMO
Immunohistochemical techniques were used to assess major histocompatibility complex (MHC) class II expression by enterocytes and lamina propria cells in the canine intestinal tract. Duodenal enterocyte class II expression was faint and limited to the lower crypt region whereas jejunal and ileal enterocyte expression was stronger, being present in both crypt and villus areas. Enterocyte staining was of greatest intensity in crypts adjacent to Peyer's patches and intense membrane staining of most Peyer's patch lymphocytes was also seen. Enterocyte MHC class II expression in the colon was largely limited to the lower crypt region. Within the lamina propria, of all intestinal sites examined, a heterogeneous population of cells were MHC class II positive and these had morphological features of macrophages and dendritic cells. Lymphocytes, plasma cells, fibroblasts and vascular endothelium were not stained. Definition of constitutive expression of MHC class II within the canine intestine may be important in identifying upregulation of this molecule in inflammatory bowel diseases.
Assuntos
Cães/imunologia , Antígenos de Histocompatibilidade Classe II/metabolismo , Intestinos/imunologia , Animais , Colo/citologia , Colo/imunologia , Cães/anatomia & histologia , Duodeno/citologia , Duodeno/imunologia , Epitélio/imunologia , Íleo/citologia , Íleo/imunologia , Imuno-Histoquímica , Jejuno/citologia , Jejuno/imunologia , Nódulos Linfáticos Agregados/citologia , Nódulos Linfáticos Agregados/imunologia , Distribuição TecidualRESUMO
We have isolated dendritic cells (DC) from Peyer's patches (PP) of pig small intestine by mechanical tissue disruption followed by fractionation of isolated cells on metrizamide gradients. Characterisation was carried out using the following criteria: morphology; lysosomal enzyme synthesis; expression of membrane antigens; and capacity for antigen presentation. Dendritic cells did not express acid phosphatase or beta-galactosidase, but were weakly positive for non-specific esterase and ATPase. Dendritic cells did not express CD3, CD2, sIg, or an antigen specific for pig mononuclear phagocytes and granulocytes. They did, however, express MHC class II at very high levels. They were shown to be potent stimulators in an allogeneic mixed lymphocyte reaction.
Assuntos
Separação Celular/métodos , Células Dendríticas/citologia , Nódulos Linfáticos Agregados/citologia , Fosfatase Ácida/metabolismo , Adenosina Trifosfatases/metabolismo , Animais , Anticorpos Monoclonais , Apresentação de Antígeno/imunologia , Antígenos CD/metabolismo , Centrifugação com Gradiente de Concentração , Células Dendríticas/fisiologia , Esterases/metabolismo , Feminino , Antígenos de Histocompatibilidade Classe II/metabolismo , Intestino Delgado/citologia , Masculino , Suínos , Porco Miniatura , beta-Galactosidase/metabolismoRESUMO
The distribution of major histocompatibility complex (MHC) class II positive cells within the connective tissue and the epithelium of the involuted bovine mammary gland has been determined. The effect of intramammary administration of the antigens ovalbumin and formalin killed Streptococcus uberis on the distribution pattern has also been investigated. Infusion of formalin killed S. uberis increased cellular expression of class II antigens when compared with quarters either infused with ovalbumin, not infused at all, or from which minor pathogens had been isolated. The increased expression occurred particularly in the area of the gland cistern-secretory tissue junction.
Assuntos
Bovinos/imunologia , Antígenos de Histocompatibilidade Classe II/análise , Glândulas Mamárias Animais/imunologia , Mastite Bovina/imunologia , Animais , Tecido Conjuntivo/metabolismo , Epitélio/metabolismo , Feminino , Imunidade Celular , Técnicas Imunoenzimáticas , Ovalbumina/imunologia , Streptococcus/imunologiaRESUMO
Ultrastructural, histochemical and immunohistochemical features of porcine intestinal lamina propria macrophages (LPMs), peripheral blood fibronectin-adherent cells (FACs) and splenic-adherent cells (SPACs) were compared. Freshly isolated FACs and SPACs were small and showed small cytoplasmic processes, little evidence of endocytic vacuoles, few lysosomes and sparse rough endoplasmic reticulum (RER). Fresh FACs were negative for acid phosphatase, non-specific esterase (NSE) and beta-galactosidase activity. Of the SPACs, 20-40% were positive for acid phosphatase, < 5% for NSE and 5-10% for beta-galactosidase. Pre-cultured FACs and SPACs were large and showed an abundance of endocytic vacuoles; they possessed dilated and prominent RER and > 95% were positive for the three enzyme activities. LPMs exhibited abundant endocytic vacuoles or vesicles and lysosomes but sparse RER, and > 85% were positive for the three enzymes. LPMs (24%), FACs (49%) and SPACs (40%) expressed MHC (major histocompatibility complex) class II glycoproteins. Macrophage-granulocyte antigens were detected in LPMs (14%), FACs (50%) and SPACs (33%). The results thus suggest that freshly isolated FACs differ from LPMs morphologically and in enzymic features, and the differences may represent part of the cell maturation process.
Assuntos
Mucosa Intestinal/imunologia , Macrófagos/citologia , Monócitos/citologia , Fosfatase Ácida/análise , Animais , Antígenos de Diferenciação Mielomonocítica/análise , Membrana Basal/citologia , Membrana Basal/imunologia , Carboxilesterase , Hidrolases de Éster Carboxílico/análise , Adesão Celular , Fibronectinas/fisiologia , Antígenos de Histocompatibilidade Classe II/análise , Histocitoquímica , Mucosa Intestinal/citologia , Macrófagos/enzimologia , Macrófagos/imunologia , Macrófagos/ultraestrutura , Masculino , Monócitos/enzimologia , Monócitos/imunologia , Monócitos/ultraestrutura , Baço/citologia , Suínos , beta-Galactosidase/análiseRESUMO
Leukocyte populations present in the discrete Peyer's patches (PP) of the pig were characterized from birth (Day 0) to day 35 after birth by immunohistochemistry and image analysis. Immediately after birth, cell membrane expression of CD2 and CD3, major histocompatibilty complex (MHC) class 11 (both SLA (swine leukocyte antigen) -DQ+ and SLA-DR+), CD21, 74-22-15 and surface immunoglobulin (sIg) were all demonstrable. Computer assisted morphometric techniques were used to confirm the significant expansion of these cell populations from birth onwards. The distribution of the cell types was not random but suggested a preferential retention of cells at specific sites. This implies a degree of organization of immunological cells within the discrete PP, enhancing the potential to mount immune responses in the most efficient manner.
Assuntos
Antígenos de Superfície/biossíntese , Nódulos Linfáticos Agregados/anatomia & histologia , Suínos/anatomia & histologia , Animais , Animais Recém-Nascidos , Feminino , Processamento de Imagem Assistida por Computador , Imunidade nas Mucosas , Imuno-Histoquímica/veterinária , Masculino , Nódulos Linfáticos Agregados/citologia , Nódulos Linfáticos Agregados/crescimento & desenvolvimento , Nódulos Linfáticos Agregados/imunologia , Receptores de Complemento 3d/biossíntese , Suínos/crescimento & desenvolvimento , Suínos/imunologiaAssuntos
Células Apresentadoras de Antígenos/imunologia , Interleucina-1/fisiologia , Mucosa Intestinal/imunologia , Linfócitos T/imunologia , Animais , Células Cultivadas , Concanavalina A/farmacologia , Epitélio/imunologia , Interleucina-2/farmacologia , Mucosa Intestinal/citologia , Ativação Linfocitária , Macrófagos/imunologia , Masculino , Ovalbumina/imunologia , Ratos , Baço/citologia , Linfócitos T Citotóxicos/imunologiaAssuntos
Adjuvantes Imunológicos/administração & dosagem , Antígenos/administração & dosagem , Imunoglobulina A/biossíntese , Administração Oral , Animais , Toxina da Cólera/administração & dosagem , Toxina da Cólera/imunologia , Colforsina/administração & dosagem , Colforsina/imunologia , AMP Cíclico/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Hemocianinas/administração & dosagem , Hemocianinas/imunologia , Imunidade nas Mucosas , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/imunologia , Mucosa Intestinal/metabolismo , Leucotrieno B4/administração & dosagem , Leucotrieno B4/imunologia , Camundongos , Baço/efeitos dos fármacos , Baço/imunologia , Baço/metabolismo , Fatores de Virulência de Bordetella/administração & dosagem , Fatores de Virulência de Bordetella/imunologiaRESUMO
Gut mesenchymal fibroblasts form complex phenotypical and functional populations. They participate actively in homeostatic maintenance of the extracellular matrix, epithelial barrier function, repair mechanisms and leucocyte migration. In inflammation, they become activated, change matrix expression and synthesize proinflammatory mediators. Subpopulations of mucosal fibroblasts express CD40 and the aim of this study was to define its role in their proinflammatory function. Stable primary fibroblast lines derived from normal mouse colon and inflamed colon from CD4(+) CD45RB(high)-transplanted SCID mice were used as models to explore the role of mucosal fibroblast CD40 in the inflammatory process. Phenotype correlated with in situ fibroblast phenotype in the tissues of origin. Lines from both sources co-expressed CD40 and Thy1.2 independently of alpha-smooth muscle actin. A subpopulation of CD40(+) fibroblasts from normal colon expressed CD40 at high levels and expression was enhanced by interferon (IFN)-gamma treatment, whereas all CD40(+) fibroblasts from colitis expressed at low levels and expression was unaffected by IFN-gamma treatment. Despite lower-level expression of CD40 by cells from colitis, they secreted constitutively interleukin (IL)-6 and C-C chemokine (CCL)2. Ligation of CD40 enhanced secretion of these mediators and induced secretion of CCL3. CD40 in cells from colitis was more responsive to ligation than CD40 on cells from normal tissue and this sensitivity was amplified selectively by the action of IFN-gamma. We conclude that the inflammatory milieu in colitis induces long-lasting changes in phenotype and proinflammatory function in colonic fibroblasts. In particular, proinflammatory signalling from fibroblast CD40 is amplified synergistically by the Th1 effector T cell cytokine, IFN-gamma.
Assuntos
Antígenos CD40/imunologia , Colite/imunologia , Citocinas/biossíntese , Fibroblastos/imunologia , Interferon gama/imunologia , Animais , Antígenos CD40/metabolismo , Ligante de CD40/imunologia , Linhagem Celular , Mediadores da Inflamação/imunologia , Mucosa Intestinal/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos SCID , Transdução de Sinais/imunologia , Regulação para Cima/imunologiaRESUMO
Male and female congenitally athymic nude (nu/nu) mice infected with a single cysticercoid of Hymenolepis diminuta at 6 weeks of age retain the infection for at least 33 days. In the males of their phenotypically normal litter-mates, however, a single cysticercoid infection establishes and grows but is expelled between days 11 and 17. The unresponsiveness of the nude mouse to single H. diminuta infection is evidence that the immune rejection from normal mice is thymus-dependent.
Assuntos
Cestoides/imunologia , Camundongos Nus/imunologia , Animais , Feminino , Himenolepíase/imunologia , Masculino , Camundongos , Timo/imunologia , Fatores de TempoRESUMO
Methods are described for the purification on isokinetic gradients of isolated large bowel lamina propria lymphocytes (LPL) and large bowel adenocarcinoma-infiltrating lymphocytes (TIL). The in vitro cytotoxic and proliferative responses of these lymphocytes and of peripheral blood lymphocytes from tumor patients were assayed. Neither K cell nor NK cell cytotoxic activity was detected in LPL and TIL, although both types of lytic response were present in PBL. Lectin-induced cytotoxicity was mediated by LPL and TIL populations, but their responses in this assay were reduced comparable to that of PBL. Although TIL comprised equivalent T cell proportions to PBL, the proliferative response of TIL T cells was comparatively lower. LPL and TIL populations, but their responses in this assay were reduced comparable to that of PBL. Although TIL comprised equivalent T cell proportions to PBL, the proliferative response of TIL T cells was comparatively lower. Co-culture experiments and attempts to induce suppressor cells with concanavalin A suggested that the reduced proliferative response of T cells infiltrating these tumors was not due to the action of suppressor lymphocytes.