RESUMO
A suggested minimal scheme for substrate binding by and interconversion of three forms of the catalytic sites of the ATP synthase is presented. Each binding change, that drives simultaneous interchange of the three catalytic site forms, requires a 120 degrees rotation of the gamma with respect to the beta subunits. The binding of substrate(s) at two catalytic sites is regarded as sufficing for near maximal catalytic rates to be attained. Although three sites do not need to be filled for rapid catalysis, during rapid bisite catalysis some enzyme may be transiently present with three sites filled. Forms with preferential binding for ADP and P(i) or for ATP are considered to arise from the transition state and participate in other steps of the catalysis. Intermediate forms and steps that may be involved are evaluated. Experimental evidence for energy-dependent steps and for control of coupling to proton translocation and transition state forms are reviewed. Impact of relevant past data on present understanding of catalytic events is considered. In synthesis a key step is suggested in which proton translocation begins to deform an open site so as to increase the affinity for ADP and P(i), that then bind and pass through the transition state, and yield tightly bound ATP in one binding change. ADP binding appears to be a key parameter controlling rotation during synthesis. In hydrolysis ATP binding to a loose site likely precedes any proton translocation, with proton movement occurring as the tight site form develops. Aspects needing further study are noted. Characteristics of the related MgADP inhibition of the F(1) ATPases that have undermined many observations are summarized, and relations of three-site filling to catalysis are assessed.
Assuntos
ATPases Translocadoras de Prótons/metabolismo , Complexos de ATP Sintetase , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Sítios de Ligação , Hidrólise , Proteínas Motores Moleculares , Complexos Multienzimáticos/metabolismo , Fosfatos/metabolismo , Fosfotransferases (Aceptor do Grupo Fosfato)/metabolismo , Ligação Proteica , Conformação Proteica , PrótonsRESUMO
This paper gives an overview of a lecture scheduled for the opening of the 10th European Bioenergetics Congress. In this lecture I plan to first reflect on the accomplishments of some of the individuals who were involved in research on the ATP synthase during the past 50 years. Then I will give a brief view of the present information about rotational catalysis by the ATP synthase. This will be followed by a discussion of some results from my laboratory that call for additional experimentation. Finally I will direct attention to other questions about the ATP synthase that should be addressed in future studies.
Assuntos
ATPases Translocadoras de Prótons , Animais , Catálise , Humanos , ATPases Translocadoras de Prótons/metabolismoRESUMO
Previous studies have shown that the initial complex formed when ADP binds to nucleotide-depleted F1-ATPase is transformed with a half time of 2 to 3 min to form with a much lower rate of ADP release. The ADP binding results in a strong inhibition of ATPase activity. The present paper reports appraisal of where the inhibitory ADP binds by use of the photoreactive ADP analog, 2-N3-ADP. In presence of Mg2+ the 2-N3-ADP like ADP induces reversible inhibition of nucleotide-depleted F1 (ndF1) with a Kd of about 10 nM. Photoirradiation of the inactive 2-N3-[beta-32P]ADP-ndF1 complex results in labeling of only the beta-subunit. The major labeled peptide isolated from a trypic digest consists of residues from Ala-338 to Arg-356, with Tyr-345 as the site of labeling. This identifies the site of the inhibitory ADP binding as one of the catalytic sites of the enzyme.
Assuntos
Difosfato de Adenosina/metabolismo , Mitocôndrias Cardíacas/enzimologia , ATPases Translocadoras de Prótons/metabolismo , Difosfato de Adenosina/análogos & derivados , Difosfato de Adenosina/farmacologia , Marcadores de Afinidade , Sequência de Aminoácidos , Animais , Azidas/metabolismo , Sítios de Ligação , Bovinos , Cinética , Dados de Sequência Molecular , NADP/metabolismo , Fragmentos de Peptídeos/isolamento & purificação , ATPases Translocadoras de Prótons/antagonistas & inibidores , TripsinaRESUMO
Reaction of the multisubunit F1 ATPase from Escherichia coli (EF1) with a bifunctional cleavable crosslinker, 3,3'-dithiobis(succinimidylpropionate) (DSP), has been used to explore the possibility that during catalysis a rotational movement of catalytic subunits relative to noncatalytic subunits occurs. The premise is that such rotational catalysis is tenable if intersubunit crosslinking of a major subunit with one of the minor subunits inhibits the enzyme activity and if upon cleavage of the crosslinks, the enzyme regains activity. The results presented in this paper show that crosslinking of about 5-6 reactive groups on EF1 with DSP is accompanied by a loss of 2/3 of the enzyme activity. Both intra- and intersubunit crosslinks are formed. The most prominent intersubunit crosslinks are those of gamma and delta subunits with the alpha subunit. Nearly complete recovery of activity can be attained by cleaving the disulfide bond in the crosslinker with dithiothreitol. Because the chemical modification of enzyme groups remains after the crosslinker is cleaved, the loss in activity before cleavage can be ascribed to conformational restraints. The results show that catalysis by the EF1 ATPase is highly sensitive to the restrictions of crosslinking, and are consistent with the view that catalysis is accompanied by appreciable movements of the major subunits with respect to the minor subunits, as suggested for rotational catalysis.
Assuntos
Reagentes de Ligações Cruzadas/farmacologia , Escherichia coli/enzimologia , ATPases Translocadoras de Prótons/antagonistas & inibidores , Succinimidas/farmacologia , Ditiotreitol/farmacologia , Cinética , Substâncias Macromoleculares , ATPases Translocadoras de Prótons/isolamento & purificaçãoRESUMO
Sarcoplasmic reticulum vesicles treated with dimethylsuberimidate lose the capacity for ATP-promoted Ca2+ accumulation and show other properties indicative of leaky vesicles. As an aid to assessing whether this effect was caused by cross-linking or by hydrolysis products, characteristics of dimethylsuberimidate hydrolysis under incubation conditions used were measured. At pH 7.0, 25 DEGREES C, dimethylsuberimidate is hydrolyzed with an apparent first order rate constant of 0.016 min-1, to give dimethylsuberate as the principal product. The effect on ATP-promoted Ca2+ accululation was shown to be caused by partially and fully hydrolyzed products of the diimido ester, and not to cross-linking of membrane components.
Assuntos
Ácidos Carboxílicos/farmacologia , Imidas/farmacologia , Retículo Sarcoplasmático/metabolismo , Trifosfato de Adenosina/farmacologia , Animais , Transporte Biológico Ativo , Butiratos/farmacologia , Cálcio/metabolismo , Cinética , Membranas/efeitos dos fármacos , Membranas/metabolismo , Retículo Sarcoplasmático/efeitos dos fármacos , Fatores de TempoRESUMO
Acceptance of a membrane potential and/or a proton gradient as a possible means of transmitting energy from oxidations to ATP synthesis rests in part on a satisfactory hypothesis for how the potential or proton gradient could drive ATP synthesis. Recognition that energy input may drive ATP synthesis by change in binding of reactants at the catalytic site has led to the suggestions presented in this paper. These are that in oxidative phosphorylation and photophosphorylation, the requisite conformational changes may be coupled to exposure of charged groups to different sides of the membrane. The cycle of charged group exposure or movement may be driven by the membrane potential or, through protonation and deprotonation, may be coupled to proton translocation across the membrane. Effects of proton gradient and membrane potential may be additive. Similar conformational coupling suggestions may explain proton translocation coupled to ATP cleavage and active transport of metabolites coupled to membrane potential, proton gradients of ATP cleavage.
Assuntos
Trifosfato de Adenosina/biossíntese , Transporte Biológico Ativo , Potenciais da Membrana , Modelos Biológicos , Prótons , Metabolismo Energético , Ponto Isoelétrico , Cinética , Membranas/metabolismo , Membranas/ultraestrutura , Fosforilação OxidativaRESUMO
Energized submitochondrial particles were subjected to high or low [3H]ATP/[3H]ADP ratios, maintained during steady state by a pyruvate kinase or hexokinase regenerating system, respectively. Under both steady state conditions, about 1.4 mol [3H]nucleotide/mol ATPase was retained but considerably more [3H]ATP was retained with the high [3H]ATP/[3H]ADP ratio. The ATPase activity and the oxygen exchange of these differentially labeled SMP were the same, suggesting a lack of control function of non-catalytic tightly bound nucleotides.
Assuntos
Difosfato de Adenosina/metabolismo , Adenosina Trifosfatases/metabolismo , Trifosfato de Adenosina/metabolismo , Mitocôndrias/enzimologia , Animais , Sítios de Ligação , Catálise , Bovinos , Fenômenos Químicos , Química , Consumo de OxigênioRESUMO
Guanosine triphosphate and formycin triphosphate (FTP) in the presence of excess Mg2+ can bind to empty non-catalytic sites of spinach chloroplast ATPase (CF1). This results in a greatly reduced capacity for ATP hydrolysis compared to the enzyme with non-catalytic sites filled with ATP. With two GTP bound at non-catalytic sites the inhibition is about 90%; with two FTP bound about 80% inhibition is obtained. Binding and release of the nucleotides from the non-catalytic sites are relatively slow processes. Exposure of CF1 with one or two empty non-catalytic sites to 5-10 microM FTP or GTP for 15 min suffices for about 50% of the maximum inhibition. Reactivation of CF1 after exposure to higher FTP or GTP concentrations requires long exposure to 2 microM EDTA. The findings show that, contrary to previous assumptions, GTP can bind tightly to non-catalytic sites of CF1. They suggest that the presence of adenine nucleotides at non-catalytic sites might be essential for high catalytic capacity of the F1 ATPases.
Assuntos
Cloroplastos/enzimologia , Formicinas/metabolismo , Guanosina Trifosfato/metabolismo , ATPases Translocadoras de Prótons/antagonistas & inibidores , Ribonucleotídeos/metabolismo , Difosfato de Adenosina/fisiologia , Trifosfato de Adenosina/metabolismo , Bicarbonatos , Sítios de Ligação , Cálcio/fisiologia , Temperatura Alta , Cinética , Magnésio/fisiologia , Plantas/enzimologiaRESUMO
Under appropriate conditions tight, noncovalent binding of 2-azido-adenine nucleotides to either catalytic or noncatalytic binding sites on the E. coli F1-ATPase occurs. After removal of unbound ligands, UV-irradiation results primarily in the covalent incorporation of nucleotide moieties into the beta-subunit in both catalytic and noncatalytic site labeling experiments. Minor labeling of the alpha-subunit was also observed. After trypsin digestion and purification of the labeled peptides, microsequencing studies identified two adjacent beta-subunit tryptic peptides labeled by 2-azido-ADP or -ATP. These beta-subunit peptides were labeled on tyrosine-331 (catalytic sites) and tyrosine-354 (noncatalytic sites) in homology with the labeling patterns of the mitochondrial and chloroplast enzymes.
Assuntos
Trifosfato de Adenosina/análogos & derivados , Azidas/metabolismo , Escherichia coli/enzimologia , ATPases Translocadoras de Prótons/metabolismo , Trifosfato de Adenosina/metabolismo , Marcadores de Afinidade , Sequência de Aminoácidos , Sítios de Ligação , Fragmentos de Peptídeos/metabolismo , TripsinaRESUMO
Exposure of chloroplast F1 ATPase to 2-azido-ATP results in the noncovalent tight binding of 2-azido-ATP or 2-azido-ADP to noncatalytic or to catalytic sites. Subsequent photolysis results in covalent labeling of adjacent tryptic peptides of the beta-subunit. Binding at noncatalytic sites results in labeling of tyrosine 385 by an ATP or an ADP moiety. Binding at catalytic sites results in labeling of tyrosine 362 by only an ADP moiety. Similar labeling patterns are observed for the heat-activated or the membrane-bound enzymes.
Assuntos
Nucleotídeos de Adenina/metabolismo , Cloroplastos/enzimologia , ATPases Translocadoras de Prótons/metabolismo , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/metabolismo , Marcadores de Afinidade , Sítios de Ligação , Fragmentos de Peptídeos/análise , Mapeamento de Peptídeos , FotoquímicaRESUMO
The mechanism by which ATP is synthesized during oxidative and photophosphorylation has been elucidated by oxygen exchange and other studies: a novel form of catalysis termed rotary catalysis-is involved.