RESUMO
Cartilage fibrils contain collagen II as the major constituent, but the presence of additional components, minor collagens, and noncollagenous glycoproteins is thought to be crucial for modulating several fibril properties. We have examined the distribution of two fibril constituents-decorin and collagen IX-in samples of fibril fragments obtained after bovine cartilage homogenization. Decorin was preferentially associated with a population of thicker fibril fragments from adult articular cartilage, but was not present on the thinnest fibrils. The binding was specific for the gap regions of the fibrils, and depended on the decorin core protein. Collagen IX, by contrast, predominated in the population with the thinnest fibrils, and was scarce on wider fibrils. Double-labeling experiments demonstrated the coexistence of decorin and collagen IX in some fibrils of intermediate diameter, although most fibril fragments from adult cartilage were strongly positive for one component and lacked the other. Fibril fragments from fetal epiphyseal cartilage showed a different pattern, with decorin and collagen IX frequently colocalized on fragments of intermediate and large diameters. Hence, the presence of collagen IX was not exclusive for fibrils of small diameter. These results establish that articular cartilage fibrils are biochemically heterogeneous. Different populations of fibrils share collagen II, but have distinct compositions with respect to macromolecules defining their surface properties.
Assuntos
Cartilagem Articular/química , Colágeno/análise , Proteoglicanas/análise , Animais , Cartilagem Articular/ultraestrutura , Bovinos , Decorina , Proteínas da Matriz Extracelular , Técnicas ImunoenzimáticasRESUMO
The distribution of collagen XI in fibril fragments from 17-d chick embryo sternal cartilage was determined by immunoelectron microscopy using specific polyclonal antibodies. The protein was distributed throughout the fibril fragments but was antigenically masked due to the tight packing of collagen molecules and could be identified only at sites where the fibril structure was partially disrupted. Collagens II and IX were also distributed uniformly along fibrils but, in contrast to collagen XI, were accessible to the antibodies in intact fibrils. Therefore, cartilage fibrils are heterotypically assembled from collagens II, IX, and XI. This implies that collagen XI is an integral component of the cartilage fibrillar network and homogeneously distributed throughout the tissue. This was confirmed by immunofluorescence.
Assuntos
Cartilagem/análise , Colágeno/análise , Acetatos , Ácido Acético , Animais , Cartilagem/ultraestrutura , Embrião de Galinha , Colágeno/imunologia , Epitopos/análise , Imuno-Histoquímica , Microscopia Eletrônica , Pepsina A , TripsinaRESUMO
Chondrocytes in dense suspension culture in agarose survive in serum-free DME because they secrete low molecular mass compounds supporting their own viability. This activity can be replaced by pyruvate, or sulfhydryl compounds, e.g., cysteine or dithioerythritol. Catalase, an enzyme decomposing H2O2, also protects the cells, whereas superoxide dismutase has no effect. Therefore, chondrocytes in culture are sensitive to toxic compounds derived from molecular oxygen, i.e., hydroxyl radicals or hydrogen peroxide spontaneously generated in DME containing ascorbate and ferrous ions. Poly-ADP-ribosylation is an important step in the cascade of events triggered by these compounds. To survive, chondrocytes do not require stimulation by growth factors. They remain resting cells in fully defined, serum-free culture also at low density. Proliferation and hypertrophy can be induced by serum, but not by low cell density alone.
Assuntos
Cartilagem/citologia , Animais , Benzamidas/farmacologia , Cartilagem/efeitos dos fármacos , Catalase/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Embrião de Galinha , Colágeno/biossíntese , Colágeno/isolamento & purificação , Meios de Cultura , Cisteína/farmacologia , Cinética , Piruvatos/farmacologia , Superóxido Dismutase/farmacologiaRESUMO
The tissue distribution of type II and type IX collagen in 17-d-old chicken embryo was studied by immunofluorescence using polyclonal antibodies against type II collagen and a peptic fragment of type IX collagen (HMW), respectively. Both proteins were found only in cartilage where they were co-distributed. They occurred uniformly throughout the extracellular matrix, i.e., without distinction between pericellular, territorial, and interterritorial matrices. Tissues that undergo endochondral bone formation contained type IX collagen, whereas periosteal and membranous bones were negative. The thin collagenous fibrils in cartilage consisted of type II collagen as determined by immunoelectron microscopy. Type IX collagen was associated with the fibrils but essentially was restricted to intersections of the fibrils. These observations suggested that type IX collagen contributes to the stabilization of the network of thin fibers of the extracellular matrix of cartilage by interactions of its triple helical domains with several fibrils at or close to their intersections.
Assuntos
Cartilagem/ultraestrutura , Colágeno/fisiologia , Matriz Extracelular/ultraestrutura , Animais , Embrião de Galinha , Imunofluorescência , Ouro , Microscopia Eletrônica/métodos , Distribuição TecidualRESUMO
In bone forming cartilage in vivo, cells undergo terminal differentiation, whereas most of the cells in normal articular cartilage do not. Chondrocyte hypertrophy can be induced also in vitro by diffusible signals. We have identified growth factors or hormones acting individually on 17-d chick embryo sternal chondrocytes cultured in agarose gels under strictly serum-free conditions. Insulin-like growth factor I or insulin triggered the first steps of chondrocyte maturation, i.e., cell proliferation and increased matrix deposition while the chondrocytic phenotype was maintained. However, cells did not progress to the hypertrophic stage. Proliferation and stimulated collagen production was preceded by a lag period, indicating that synthesis of other components was required before cells became responsive to insulin-like growth factor I or insulin. Very small amounts of FBS exerted effects similar to those of insulin-like growth factor I or insulin. However, FBS could act directly and elicited hypertrophy when constituting greater than 1% of the culture media. Basic FGF has been claimed to be the most potent chondrocyte mitogen, but had negligible effects under serum-free conditions. The same is true for PDGF, a major serum-mitogen. Under the direction of thyroxine, cells did not proliferate but became typical hypertrophic chondrocytes, extensively synthesizing collagen X and alkaline phosphatase.
Assuntos
Cartilagem/citologia , Colágeno/biossíntese , Fator de Crescimento Insulin-Like I/farmacologia , Insulina/farmacologia , Tiroxina/farmacologia , Animais , Cartilagem/efeitos dos fármacos , Cartilagem/metabolismo , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Embrião de Galinha , Meios de Cultura Livres de Soro , Sangue Fetal , Fator 2 de Crescimento de Fibroblastos/farmacologia , Fator de Crescimento Derivado de Plaquetas/farmacologia , Proteoglicanas/biossínteseRESUMO
It has recently become apparent that collagen fibrils may be composed of more than one kind of macromolecule. To explore this possibility, we developed a procedure to purify fibril fragments from 17-d embryonic chicken sternal cartilage. The fibril population obtained shows, after negative staining, a uniformity in the banding pattern and diameter similar to the fibrils in situ. Pepsin digestion of this fibril preparation releases collagen types II, IX, and XI in the proportion of 8:1:1. Rotary shadowing of the fibrils reveals a d-periodic distribution of 35-40-nm long projections, each capped with a globular domain, which resemble in form and dimensions the aminoterminal globular and collagenous domains, NC4 and COL3, of type IX collagen. The monoclonal antibody (4D6) specific for an epitope close to the amino terminal of the COL3 domain of type IX collagen bound to these projections, thus confirming their identity. Type IX collagen is therefore distributed in a regular d-periodic arrangement along cartilage fibrils, with the chondroitin sulfate chain of type IX collagen in intimate contact with the fibril.
Assuntos
Cartilagem/análise , Colágeno/análise , Animais , Cartilagem/ultraestrutura , Centrifugação , Embrião de Galinha , Eletroforese em Gel de Poliacrilamida , Microscopia EletrônicaRESUMO
Primary chondrocytes from whole chick embryo sterna can be maintained in suspension culture stabilized with agarose for extended periods of time. In the absence of FBS, the cells remain viable only when seeded at high densities. They do not proliferate at a high rate but they deposit extracellular matrix with fibrils resembling those of authentic embryonic cartilage in their appearance and collagen composition. The cells exhibit many morphological and biochemical characteristics of resting chondrocytes and they do not produce collagen X, a marker for hypertrophic cartilage undergoing endochondral ossification. At low density, cells survive in culture without FBS when the media are conditioned by chondrocytes grown at high density. Thus, resting cartilage cells in agarose cultures can produce factors required for their own viability. Addition of FBS to the culture media leads to profound changes in the phenotype of chondrocytes seeded at low density. Cells form colonies at a high rate and assume properties of hypertrophic cells, including the synthesis of collagen X. They extensively deposit extracellular matrix resembling more closely that of adult rather than embryonic cartilage.
Assuntos
Cartilagem/citologia , Animais , Cartilagem/metabolismo , Cartilagem/ultraestrutura , Divisão Celular , Células Cultivadas , Embrião de Galinha , Colágeno/biossíntese , Meios de Cultura , DNA/análise , Replicação do DNA , Cinética , Microscopia Eletrônica , Proteoglicanas/biossíntese , SefaroseRESUMO
The effect of parathyroid hormone (PTH) in vivo after secretion by the parathyroid gland is mediated by bioactive fragments of the molecule. To elucidate their possible role in the regulation of cartilage matrix metabolism, the influence of the amino-terminal (NH2-terminal), the central, and the carboxyl-terminal (COOH-terminal) portion of the PTH on collagen gene expression was studied in a serum free cell culture system of fetal bovine and human chondrocytes. Expression of alpha1 (I), alpha1 (II), alpha1 (III), and alpha1 (X) mRNA was investigated by in situ hybridization and quantified by Northern blot analysis. NH2-terminal and mid-regional fragments containing a core sequence between amino acid residues 28-34 of PTH induced a significant rise in alpha1 (II) mRNA in proliferating chondrocytes. In addition, the COOH-terminal portion (aa 52-84) of the PTH molecule was shown to exert a stimulatory effect on alpha1 (II) and alpha1 (X) mRNA expression in chondrocytes from the hypertrophic zone of bovine epiphyseal cartilage. PTH peptides harboring either the functional domain in the central or COOH-terminal region of PTH can induce cAMP independent Ca2+ signaling in different subsets of chondrocytes as assessed by microfluorometry of Fura-2/AM loaded cells. These results support the hypothesis that different hormonal effects of PTH on cartilage matrix metabolism are exerted by distinct effector domains and depend on the differentiation stage of the target cell.
Assuntos
Colágeno/genética , Lâmina de Crescimento/citologia , Hormônio Paratireóideo/farmacologia , Fragmentos de Peptídeos/farmacologia , Animais , Northern Blotting , Cálcio/metabolismo , Bovinos , Divisão Celular/fisiologia , Células Cultivadas/efeitos dos fármacos , Células Cultivadas/fisiologia , Criança , Colágeno/biossíntese , Feto/citologia , Fura-2 , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Lâmina de Crescimento/patologia , Humanos , Hipertrofia , Hibridização In Situ , Hormônio Paratireóideo/química , Fenótipo , RNA Mensageiro/metabolismoRESUMO
The kinetics of triple helix formation of procollagen I were measured in normal human fibroblast cultures and cell strains from six patients with osteogenesis imperfecta (OI), a heritable connective tissue disorder. After a 4-minute pulse-labelling with [35S]methionine, the appearance of protease-resistant and thus helical collagen molecules was followed for variable chase times. In control cells, 50% of the molecules were fully triple-helical after 14 minutes. In the six OI cell strains harbouring a single Gly-->Cys substitution at positions 94, 223, 526, 691 and 988 in the helical domain of the alpha 1(I)-chain, formation of full-length protease-resistant molecules containing two mutant alpha 1(I)-chains as judged by the appearance of disulphide-linked alpha 1(I)-dimers was delayed by 5 to 60 minutes. The delay inversely correlated with the thermal stability of abnormal collagen molecules containing alpha 1(I)-dimers. Folding time and melting temperature of procollagen I in the sixth cell strain with a Gly-->Cys substitution at position 1017, outside the triple helical region in the C-terminal telopeptide, were normal. Here, we demonstrate the hitherto postulated delay in the zipper-like folding of collagen molecules harbouring Gly-->Cys substitutions in the alpha 1(I)-chain affecting the helical part of the molecule.
Assuntos
Colágeno/química , Cisteína , Glicina , Osteogênese Imperfeita/genética , Mutação Puntual , Pró-Colágeno/química , Dobramento de Proteína , Estrutura Secundária de Proteína , Pele/metabolismo , Sequência de Aminoácidos , Células Cultivadas , Colágeno/genética , Colágeno/metabolismo , Fibroblastos/metabolismo , Cinética , Osteogênese Imperfeita/metabolismo , Pró-Colágeno/genética , Pró-Colágeno/metabolismo , Valores de ReferênciaRESUMO
The rapidly increasing knowledge about the molecular biology of the extracellular matrix has changed the concepts for the pathomechanisms of heritable connective tissue diseases. The spectrum of genetic matrix disorders is much broader than previously thought and now also includes diseases of organs such as the kidney, eye, and muscles. In addition, evidence is emerging that certain "acquired" diseases may be inherited, and that defects in signal transduction and patterning genes contribute to the pathology of connective tissue disorders. The phenotypes of genetic matrix disorders are determined by basic biological characteristics of the extracellular matrix. (a) The extracellular matrix occurs ubiquitously and is important for organ development and functions. (b) Matrix macromolecules are often large oligomers that polymerize into suprastructures at several hierarchic levels. They form insoluble fibrils or filaments that are further assembled into tissue suprastructures, for example, bundles or networks of fibrils. (c) Matrix suprastructures share characteristics with metal alloys. Tissue-specific mixtures of matrix molecules form specific arrays that differ from those of the pure components. Therefore the phenotypes of matrix diseases reflect a cascade of pathological events disturbing alloy formation, such as abnormal protein synthesis and folding, defective fibrillogenesis, and bundling, all capable of leading to abnormal cell-matrix interactions.
Assuntos
Doenças do Tecido Conjuntivo/genética , Matriz Extracelular/genética , Proteínas da Matriz Extracelular/genética , HumanosRESUMO
Skin of patients with severe generalized recessive dystrophic epidermolysis bullosa (SGRDEB) was studied by immunoelectron microscopy and immunoblotting with antibodies to type VII collagen, a major structural component of anchoring fibrils. In normal skin, the protein was localized to the dermoepidermal junction zone below the basement membrane and was extractable from the papillary dermis after artificial epidermolysis. In SGRDEB skin, neither immunoreactive material below the basement membrane nor identifiable anchoring fibrils could be recognized and neither the tissue form nor the specific proteolytic fragments of type VII collagen were found in extracts of SGRDEB skin. Very low amounts of type VII collagen alpha-chains could be detected in cultures of SGRDEB-fibroblasts, whereas normal fibroblasts synthesized more of this collagen. These results suggest that a genetic defect in the correct synthesis, secretion, or in the molecular assembly of type VII collagen may underlie SGRDEB.
Assuntos
Colágeno/metabolismo , Epidermólise Bolhosa/patologia , Pele/patologia , Adolescente , Adulto , Colágeno/classificação , Epidermólise Bolhosa/genética , Epidermólise Bolhosa/metabolismo , Fibroblastos/metabolismo , Fibroblastos/patologia , Genes Recessivos , Humanos , Imuno-Histoquímica , Microscopia Eletrônica , Pele/metabolismoRESUMO
Endochondral ossification in growth plates proceeds through several consecutive steps of late cartilage differentiation leading to chondrocyte hypertrophy, vascular invasion, and, eventually, to replacement of the tissue by bone. The subchondral vascular system is essential for this process and late chondrocyte differentiation is subject to negative control at several checkpoints. Endothelial cells of subchondral blood vessels not only are the source of vascular invasion accompanying the transition of hypertrophic cartilage to bone but also produce factors overruling autocrine barriers against late chondrocyte differentiation. Here, we have determined that the action of proteases secreted by endothelial cells were sufficient to derepress the production of the hypertrophy-markers collagen X and alkaline phosphatase in arrested populations of chicken chondrocytes. Signalling by thyroid hormones was also necessary but endothelial factors other than proteinases were not. Negative signalling by PTH/PTHrP- or TGF-beta-receptors remained unaffected by the endothelial proteases whereas signalling by FGF-2 did not suppress, but rather activated late chondrocyte differentiation under these conditions. A finely tuned balance between chondrocyte-derived signals repressing cartilage maturation and endothelial signals promoting late differentiation of chondrocytes is essential for normal endochondral ossification during development, growth, and repair of bone. A dysregulation of this balance in permanent joint cartilage also may be responsible for the initiation of pathological cartilage degeneration in joint diseases.
Assuntos
Condrócitos/citologia , Endopeptidases/metabolismo , Endotélio Vascular/enzimologia , Proteínas/metabolismo , Transdução de Sinais , Fosfatase Alcalina/metabolismo , Animais , Cartilagem Articular/citologia , Diferenciação Celular , Células Cultivadas , Embrião de Galinha , Condrócitos/metabolismo , Colágeno/metabolismo , Endotélio Vascular/citologia , Fator 2 de Crescimento de Fibroblastos/metabolismo , Fator 2 de Crescimento de Fibroblastos/farmacologia , Humanos , Hormônio Paratireóideo/metabolismo , Hormônio Paratireóideo/farmacologia , Proteína Relacionada ao Hormônio Paratireóideo , Fragmentos de Peptídeos/metabolismo , Fragmentos de Peptídeos/farmacologia , Receptor Tipo 1 de Hormônio Paratireóideo , Receptores de Hormônios Paratireóideos/metabolismo , Artéria Subclávia/metabolismo , Suínos , Tiroxina/metabolismo , Tiroxina/farmacologia , Fator de Crescimento Transformador beta/metabolismo , Fator de Crescimento Transformador beta/farmacologia , Fator de Crescimento Transformador beta2RESUMO
Indirect immunofluorescence staining of normal skin with affinity-purified antibodies revealed a conspicuous presence of collagen XVI at the dermo-epidermal interface where it occurs in close vicinity to collagen VII. In addition, the protein co-localizes with fibrillin 1 at the cutaneous basement membrane zone and the adjacent papillary dermis, but not in deeper layers of the dermis. Both fibronectin and collagen XVI are distributed throughout smooth muscles of hair follicles but do not co-localize. These data suggest, therefore, that collagen XVI contributes to the structural integrity of the dermo-epidermal junction zone by interacting with components of the anchoring complexes and the microfibrillar apparatus. A strong immunofluorescence signal associated with the extracellular matrix of individual cells was observed for keratinocytes or fibroblasts in monolayer cultures. Therefore, both cell types are likely sources of the protein also in situ. The rate of expression of collagen XVI mRNA in keratinocytes is about half of that in normal human skin fibroblasts. In both cell types, TGF-beta2 treatment results in an up-regulation of the collagen XVI-mRNA by approximately 50%. In keratinocytes, synthesis of collagen XVI protein and deposition to the cell layer and the extracellular matrix is stimulated fivefold and twofold, respectively. Since TGF-beta2 also upregulates the biosynthesis of other matrix macromolecules in the subepidermal zone the factor is likely to contribute to the stabilization of matrix zones near basement membranes in healing wounds.
Assuntos
Colágeno/biossíntese , Fibroblastos/metabolismo , Queratinócitos/metabolismo , Células Cultivadas , Colágeno/genética , Colágeno/metabolismo , Derme/citologia , Matriz Extracelular/metabolismo , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Regulação da Expressão Gênica , Humanos , Queratinócitos/citologia , Queratinócitos/efeitos dos fármacos , RNA Mensageiro , Fator de Crescimento Transformador beta/metabolismo , Fator de Crescimento Transformador beta/farmacologiaRESUMO
Late cartilage differentiation during endochondral bone formation is a multistep process. Chondrocytes transit through a differentiation cascade under the direction of environmental signals that either stimulate or repress progression from one step to the next. In human costal cartilage, chondrocytes reach very advanced stages of late differentiation and express collagen X. However, remodeling of the tissue into bone is strongly repressed. The second hypertrophy marker, alkaline phosphatase, is not expressed before puberty. Upon sexual maturity, both alkaline phosphatase and collagen X activity levels are increased and slow ossification takes place. Thus, the expression of the two hypertrophy markers is widely separated in time in costal cartilage. Progression of endochondral ossification in this tissue beyond the stage of hypertrophic cartilage appears to be associated with the expression of alkaline phosphatase activity. Costal chondrocytes in culture are stimulated by parathyroid hormone in a PTH/PTHrP receptor-mediated manner to express the fully differentiated hypertrophic phenotype. In addition, the hormone stimulates hypertrophic development even more powerfully through its carboxyterminal domain, presumably by interaction with receptors distinct from PTH/PTHrP receptors. Therefore, PTH can support late cartilage differentiation at very advanced stages, whereas the same signal negatively controls the process at earlier stages.
Assuntos
Cartilagem Articular/crescimento & desenvolvimento , Condrócitos/citologia , Osteogênese/fisiologia , Costelas/crescimento & desenvolvimento , Fosfatase Alcalina/biossíntese , Cartilagem Articular/metabolismo , Cartilagem Articular/fisiologia , Diferenciação Celular , Divisão Celular , Células Cultivadas , Condrócitos/metabolismo , Condrócitos/fisiologia , Colágeno/biossíntese , Humanos , Masculino , Costelas/citologia , Costelas/metabolismo , Costelas/fisiologia , Fatores de TempoRESUMO
During endochondral bone formation, cells in the emerging cartilaginous model transit through a cascade of several chondrocyte differentiation stages, each characterized by a specific expression repertoire of matrix macromolecules, until, as a final step, the hypertrophic cartilage is replaced by bone. In many permanent cartilage tissues, however, late differentiation of chondrocytes does not occur, due to negative regulation by the environment of the cells. Here, addressing the reason for the difference between chondrocyte fates in the chicken embryo sternum, cells from the caudal and cranial part were cultured separately in serum-free agarose gels with complements defined earlier that either permit or prevent hypertrophic development. Total RNA was extracted using a novel protocol adapted to agarose cultures, and the temporal changes in developmental stage-specific mRNA expression were monitored by Northern hybridization and phosphor image analysis. Kinetic studies of the mRNA accumulation not only showed significant differences between the expression patterns of cranial and caudal cultures after recovery, but also revealed two checkpoints of chondrocyte differentiation in keeping with cartilage development in vivo. Terminal differentiation of caudal chondrocytes is blocked at the late proliferative stage (stage Ib), while the cranial cells can undergo hypertrophic development spontaneously. The differentiation of cranial chondrocytes is reversible, since they can re-assume an early proliferative (stage Ia) phenotype under the influence of insulin, fibroblast growth factor-2 and transforming growth factor-beta in combination. Thus, the expression pattern in the latter culture resembles that of articular chondrocytes. We also provide evidence that the capacities of caudal and sternal chondrocytes to progress from the late proliferative (stage Ib) to hypertrophic stage (stage II) correlate with their differing abilities to express the Indian hedgehog gene.
Assuntos
Condrócitos/citologia , Transativadores , Animais , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Embrião de Galinha , Condrócitos/efeitos dos fármacos , Fator 2 de Crescimento de Fibroblastos/farmacologia , Marcadores Genéticos , Proteínas Hedgehog , Insulina/farmacologia , Fenótipo , Proteínas/genética , RNA/isolamento & purificação , Fator de Crescimento Transformador beta/farmacologiaRESUMO
Collagens are the major proteinaceous constituents of cartilage. Three collagen types participate in the formation of striated fibrils of cartilage, collagens II, IX, and XI. Collagen II and XI belong to the subgroup of fibrillar collagens and are structurally closely related, differing mainly in their N-propeptides. Collagen IX has a very different structure but is nevertheless an essential constituent of the striated fibrils. Two other collagen types are also found in cartilage but form distinct structures. Collagen VI, found mainly in the periphery of the chondrocytes, forms beaded filaments. These filaments are probably formed by interaction of collagen VI with hyaluronan. Collagen X is expressed by hypertrophic chondrocytes. It has been shown to form in vitro hexagonal lattices and in vivo to be associated either with striated fibrils or with mats which may correspond to the lattices. The functional role of the collagen diversity in cartilage is discussed.
Assuntos
Cartilagem Articular/química , Colágeno/química , Colágeno/fisiologia , Animais , Cartilagem Articular/fisiologia , Humanos , Relação Estrutura-AtividadeRESUMO
Mechanical conditions at the fracture line determine the mode of fracture healing (osteonal versus non-osteonal bone union). The aim of this study was to investigate the influence of differing degrees of fracture stability on the time course of chondrogenesis, enchondral ossification and immigration of macrophages into the fracture callus. Using a fracture model of the rat's tibia, histological (Azan staining), immunohistological (antibodies directed against the macrophage-specific surface antigen ED2), and molecular biological techniques (expression of the mRNA of the cartilage-specific collagen IX, osteocalcin - a marker for mature osteoblasts - and the macrophage-specific macrosialin) were employed. In terms of histology and molecular biology (collagen IX mRNA expression) chondrogenesis in the fracture gap continued for longer in less stable fractures. In more stable fractures bone formation - identified by osteocalcin mRNA expression - increased from day 12 onwards. The expression of the macrophage-specific surface antigen ED2 and the mRNA of macrosialin was more pronounced but of shorter duration in the more stable fractures. This study shows that differing degrees of fracture stability not only influence the interplay between osteogenesis and chondrogenesis but also alter the kinetics of macrophage immigration into the fracture callus. These findings could aid in better understanding the cytobiologic mechanisms of callus formation and may suggest that macrophages are an important factor not only in soft tissue healing but also in bone healing.
Assuntos
Condrogênese/fisiologia , Macrófagos/imunologia , Osteogênese/fisiologia , Fraturas da Tíbia/fisiopatologia , Animais , Antígenos CD/genética , Antígenos de Diferenciação Mielomonocítica/genética , Antígenos de Superfície/análise , Movimento Celular/imunologia , Colágeno/genética , Consolidação da Fratura/fisiologia , Expressão Gênica/fisiologia , Imuno-Histoquímica , Macrófagos/química , Macrófagos/citologia , Masculino , Osteocalcina/genética , RNA Mensageiro/análise , Ratos , Ratos Wistar , Tíbia/fisiologia , Fraturas da Tíbia/imunologiaAssuntos
Circulação Sanguínea/efeitos dos fármacos , Heparina/farmacologia , Transplante de Rim , Transplante Heterólogo , Angiografia , Animais , Azatioprina/farmacologia , Tempo de Circulação Sanguínea , Soluções Tampão , Dactinomicina/farmacologia , Diaminas/farmacologia , Cães , Artéria Femoral/cirurgia , Glucocorticoides/farmacologia , Imunossupressores/farmacologia , Rim/irrigação sanguínea , Ácidos Nicotínicos/farmacologia , Perfusão , Procaína/administração & dosagem , Procaína/farmacologia , Inibidores de Proteases , Artéria Renal/diagnóstico por imagem , Saponinas/farmacologia , Suínos , Teofilina/farmacologiaAssuntos
Liberdade , Felicidade , Valores Sociais , Adulto , Criança , Existencialismo , Humanos , Psicologia da Criança , Responsabilidade SocialRESUMO
The passive transfer of concentrated immunoglobulins or affinity-purified anti-collagen antibodies from sera of mice with type II collagen-induced arthritis can induce erosive arthritis in recipient animals. In both cases, the incidence of arthritis was over 60% and the inflammation persisted for at least two weeks. Radiography revealed bone destruction and apposition of a newly formed material while histologic examination showed cartilage and bone degradation, accompanied with synovitis and periarthritis. Inflammatory infiltrates were composed of polymorphonuclear leucocytes and lymphocytes, and were associated with a proliferation of connective tissue cells.