RESUMO
The distribution of radioactivity in association with sympathetic nerve terminals and intraneuronal organelles 30 min after the administration of tritiated norepinephrine (NE-(3)H) was studied by electron microscope radioautography with recently developed quantitative methods of analysis reported in the accompanying paper (Salpeter et al., 1969). Nerves from the pineal body and the adrenal capsule were examined. It was found that nerve terminals containing vesicles were heavily labeled. (These terminals were not necessarily in contact with some innervated structure.) There was no selective labeling of either the intraneuronal mitochondria or the relatively small population of large ( approximately 1000 A) dense core granules. Small vesicles ( approximately 500 A), some of which have a dense internal granule, could not be analysed separately because they are closely packed and occupy approximately 60% of the volume in terminals. Because of the extensive distribution of these small granular and agranular vesicles in the radioactive terminals, they remain the most likely site for norepinephrine binding. Yet although the vesicles were uniformly distributed within the nerve terminals, it appears that the radioactivity was not. There appeared to be a somewhat higher concentration of radioactivity at the periphery of the nerve terminals than in the center. The usefulness of the method of analysis used in this study for determining the location of bound H(3)NE pools in the nerve is discussed.
RESUMO
Insulin or highly homologous transcripts is shown to be synthesized in cultures of mammalian anterior pituitary cells using cloned insulin-specific cDNA probes and nucleic acid cytochemistry. The insulin-hybridizing cells are less abundant than the growth hormone-producing cells, occurring in the cultures at approximately one tenth the frequency. Immunocytochemistry demonstrates that insulin or insulin-like proteins is also synthesized by the cultured pituitary cells and that the insulin immunoreactivity is contained within secretory granules. It appears that many of these secretory granules are concentrated around the periphery of the cell, unlike the insulin-containing granules in pancreatic B-cells.
Assuntos
Insulina/biossíntese , Hibridização de Ácido Nucleico , Adeno-Hipófise/metabolismo , Transcrição Gênica , Animais , Cricetinae , DNA/análise , Histocitoquímica , Camundongos , Microscopia Eletrônica , RNA/análise , RatosRESUMO
Fluorophosphate-reactive (FPR) sites in the adult male rat liver, tentatively identified as esterase active centers, were localized and measured using the combined techniques of quantitative electron microscope radioautography and morphometric analysis with the light and electron microscope. The FPR sites were measured in liver which had been prefixed by perfusion with 1.5% glutaraldehyde and reacted with 10(-4) M tritium-labeled diisopropyl fluorophosphate (3H-DFP). Under the experimental conditions 64-67% of the esterase activity in fresh liver was retained for reaction with the 3H-DFP, which is known to bind irreversibly to the active sites of certain esterases. In light and electron microscope radioautographs the developed silver grains were concentrated over the cytoplasm of hepatocytes. A low concentration occurred over erythrocytes. All other areas in the liver had a concentration of grains resembling the background concentration. Quantitative measurements of grain density in the electron microscope radioautographs revealed the highest density, after correcting for radiation spread, in cytoplasmic granules (mainly cytolysomes). The grain densities over the rough and smooth endoplasmic reticulum and associated structures were also equal to or above the average hepatocyte grain density. Due to the large fractional volume of endoplasmic reticulum per hepatocyte (58% of cell volume) and the fraction of the liver occupied by hepatocytes (79% of liver volume) the majority of FPR sites in the liver occurred in the rough and smooth endoplasmic reticulum and associated structures. The average numbers of FPR sites were calculated per micrometer3 of hepatocyte (5.0 x 10(5) sites/micrometer3) and per unit volume of each significantly labeled organelle. In addition, the number of FPR sites per hepatocyte (2.5 X 10(9) sites/cell), per cm3 liver (4.1 X 10(17) sites/cm3) and in the total liver of an average 100 g male rate (2.2 X 10(18) sites/total liver) were also calculated.
Assuntos
Esterases/análise , Fígado/enzimologia , Animais , Autorradiografia , Isoflurofato , Fígado/ultraestrutura , Masculino , Microscopia Eletrônica/métodos , Ratos , TrítioRESUMO
A number of studies have recently demonstrated that insulin may be synthesized outside the pancreas. The present study was designed to investigate whether insulin-like activity exists in retinal glial cells and if so, whether it is due to local synthesis of insulin. Immunocytochemical techniques using insulin antisera were applied to cultured rat retinal glial cells, and insulin-like immunoreactivity was demonstrated in the cytoplasm of these cells. In situ DNA-RNA hybridization studies using 3H-labeled rat insulin cDNA indicated that the glial cells, particularly the Müller cells of the retina also contained the mRNA necessary for de novo synthesis of insulin or a closely homologous peptide. This peptide may be important in neuromodulation or regulation of metabolism of retinal cells and capillaries.
Assuntos
Insulina/genética , Neuroglia/metabolismo , RNA Mensageiro/metabolismo , Retina/metabolismo , Animais , Linhagem Celular , Células Cultivadas , Cricetinae , DNA , Imuno-Histoquímica , Microscopia Eletrônica de Varredura , Hibridização de Ácido Nucleico , Ratos , Retina/citologia , Retina/ultraestruturaRESUMO
PURPOSE: The goal of this study was to extend the results of previous immunoassay, immunocytochemistry, and in situ hybridization studies showing the presence of insulin-related peptide in the rat retina by confirming the expression of insulin genes in the rat eye. METHODS: Total and poly(A)+ RNA were isolated from whole rat eyes, and separately from the retina, choroid, iris, lens, and vitreous. The poly(A)+ RNA was used for preparation of insulin-specific cDNA according to a coupled reverse transcription polymerase chain reaction (RT-PCR) protocol under high stringency conditions. Southern transfers, restriction fragment analyses, and nucleotide sequencing were used to characterize and identify the amplified cDNA products. RESULTS: Amplified cDNA fragments of 329 +/- 6 base pairs (bp) were derived from whole rat eye and rat retina poly(A)+ RNA, but not from other regions of the eye. Southern blots probed with preproinsulin-specific primer demonstrated homology with similar-sized cDNA from rat pancreas. Restriction digests with 10 restriction enzymes and direct nucleotide sequencing confirmed that the 329-bp cDNA was identical to the previously known coding sequence for rat pancreatic preproinsulin1 DNA. CONCLUSIONS: The identification of retinal preproinsulin1 mRNA was confirmed. This correlates with previous studies showing insulin immunoreactivity in rat eyes and in cultured retina, and verifies in situ hybridization evidence for the presence of insulin-related mRNA in retinal glial cells.
Assuntos
Proinsulina/metabolismo , Precursores de Proteínas/metabolismo , RNA Mensageiro/análise , Retina/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Southern Blotting , DNA/análise , Eletroforese em Gel de Ágar , Insulina/genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Proinsulina/genética , Precursores de Proteínas/genética , Ratos , Ratos Sprague-DawleyRESUMO
A method has been developed for measurement of myocardial infarct size from thallium-201 scintigrams that depends on computer measurement of levels of radioactivity in the myocardium. In 16 dogs, thallium-201 scintigrams were obtained in the left lateral and left anterior oblique projections 48 hours after ligation of the left anterior descending coronary artery. Scintigraphic results were obtained by two independent observers and were compared with tissue measurements of infarct volume calculated from thallium autoradiograms and nitro-blue tetrazolium (NBT)-stained tissue slices. Infarct volumes derived from tissue measurements were used to develop criteria for the computer scintigraphic technique. There was no significant difference in the scintigraphic measurements made by the two observers. Scintigraphic infarct size in the left lateral and left anterior oblique projections correlated with tissue infarct size with r values of 0.88 and 0.75, respectively, for thallium autoradiography and 0.71 and 0.70, respectively, for NBT tissue staining. The range of infarct volume was 3.3 to 14.8 percent of the left ventricular mass. Results of this study suggest that scintigraphic quantitation of infarct size is feasible in this dog model.
Assuntos
Infarto do Miocárdio/diagnóstico por imagem , Radioisótopos , Tálio , Doença Aguda , Animais , Cães , CintilografiaRESUMO
Mouse and rat retinae were examined by the peroxidase-anti-peroxidase technique of immunocytochemistry using an antiserum against glucagon. The immunoreactivity was found in the cells of the ganglion cell layer and inner nuclear layer, including Müller cells. These observations may indicate that glucagon or a similar peptide is important in neuromodulation and/or metabolism of retinal cells.
Assuntos
Glucagon/metabolismo , Retina/metabolismo , Animais , Técnicas Imunoenzimáticas , Camundongos , RatosRESUMO
The ganglion cell layer of pre- and postnatal rat retina is positive for insulin immunoreactivity. At birth the inner nuclear layer also stains for insulin. By 5 days after birth the layers characteristic of the mature retina are demonstrable. At this time the outer nuclear layer and both limiting membranes show insulin reactivity. The lens is positive for insulin at all stages studied and the retinal pigment and choroid layers are positive after birth. These observations suggest that insulin may be important in differentiation and/or maturation of the retina.
Assuntos
Animais Recém-Nascidos/metabolismo , Feto/metabolismo , Insulina/metabolismo , Retina/metabolismo , Animais , Feminino , Imuno-Histoquímica , Insulina/imunologia , Gravidez , Ratos , Ratos Endogâmicos , Retina/crescimento & desenvolvimentoRESUMO
Cells from the Y79 human retinoblastoma cell line were examined by immunofluorescence immunocytochemistry using an antiserum against insulin. All the cells showed intense staining, indicating the presence of insulin-like immunoreactivity in these cells. Our observations suggest that insulin may play an important role in the metabolism of retinoblastoma cells and that it may be possible to use this cell line as an in vitro model for studies on the action of insulin in the metabolism of human retinal cells.
Assuntos
Neoplasias Oculares/metabolismo , Insulina/metabolismo , Retinoblastoma/metabolismo , Linhagem Celular , Imunofluorescência , HumanosRESUMO
We previously reported the presence of insulin-like immunoreactivity in cells from the human retinoblastoma Y79 cell line. In the present study, in situ DNA hybridization techniques were applied, using a human insulin cDNA probe to investigate whether the insulin-like activity is due to local synthesis of insulin. Our results suggest that Y79 cells contain mRNA for the synthesis of insulin or a homologous peptide. In addition, 125I-insulin binding autoradiographic studies show that these cells also contain specific insulin-binding sites. It is suggested that insulin may play an autocrine and/or paracrine role in the maintenance and metabolism of the Y79 retinoblastoma cells.
Assuntos
Neoplasias Oculares/metabolismo , Insulina/metabolismo , RNA Mensageiro/metabolismo , Receptor de Insulina/metabolismo , Retina , Retinoblastoma/metabolismo , Células Tumorais Cultivadas/metabolismo , Autorradiografia , Humanos , Radioisótopos do Iodo , Hibridização de Ácido NucleicoRESUMO
Immunocytochemistry using peroxidase antiperoxidase (PAP) techniques showed insulin-like immunoreactivity in the human retina, and in the mouse retina and optic nerve. The immunoreaction product was seen in the inner nuclear, ganglion cell, outer and inner plexiform layers of the retinas, and in glial cell bodies of the optic nerve. A similar staining pattern using antiserum to S-100 protein, a marker for glial elements, was also seen in these tissues. This demonstrates that insulin or insulin-like immunoreactivity appears to be limited to glial cells of the retina and optic nerve. Our study suggests that the presence of insulin or a similar peptide in retina and optic nerve may be important for their normal function and metabolism.
Assuntos
Técnicas Imunoenzimáticas , Insulina/metabolismo , Retina/metabolismo , Proteínas S100/metabolismo , Animais , Humanos , Camundongos , Neuroglia/metabolismo , Nervo Óptico/citologia , Nervo Óptico/metabolismo , Retina/citologiaRESUMO
Human pituitary cells and pancreatic islet beta cells from surgical and autopsy material showed positive immunoreactivity to anti-porcine insulin antisera demonstrating the presence in these tissues of insulin or insulin-like immunoreactive material. Glutaraldehyde-fixed, one micron epoxy sections were used and the polymerized resin was removed prior to staining with the peroxidase anti-peroxidase technique using guinea pig antisera to porcine insulin. Pancreatic beta cells served as the positive control, and appropriate negative controls were also utilized.
Assuntos
Insulina/análise , Adeno-Hipófise/análise , Histocitoquímica , Humanos , Técnicas Imunoenzimáticas , Ilhotas Pancreáticas/análiseRESUMO
Many studies support the concept of insulin synthesis in tissues other than the pancreas. Our previous investigations have demonstrated the presence of insulin immunoreactivity in the adrenal medulla of the rat. This immunoreactivity was found to be associated with the chromaffin granule. This study is directed at isolating the messenger ribonucleic acid that encodes for preproinsulin. Reverse transcription coupled with polymerase chain reaction was used. Complementary deoxyribonucleic acid (cDNA) was amplified, extracted and reamplified. It was then subjected to digestion with four different restriction endonuclease enzymes. Its resemblance to the corresponding cDNA that encodes for preproinsulin I in the rat was established. Our results suggest that insulin is synthesized in the rat adrenal gland for autocrine, paracrine, neuromodulation or local physiologic function.
Assuntos
Glândulas Suprarrenais/química , Proinsulina/genética , Precursores de Proteínas/genética , RNA Mensageiro/isolamento & purificação , Sequência de Aminoácidos , Animais , Sequência de Bases , Códon , Enzimas de Restrição do DNA/metabolismo , DNA Complementar/química , DNA Complementar/metabolismo , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Insulina , Masculino , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Proinsulina/química , Precursores de Proteínas/química , DNA Polimerase Dirigida por RNA , Ratos , Ratos Sprague-Dawley , EspectrofotometriaRESUMO
The present study was undertaken to determine the feasibility of using in situ hybridization techniques to identify oncogene transcription in cultured cells. Following in situ hybridization with 32P-labeled v-src and v-Ha-ras DNA probes, src and Ha-ras related transcripts were identified in cell lines transfected with v-src and Ha-ras, respectively. In both the v-src and c-Ha-ras transfected cell lines, the number of silver grains over individual cells were significantly higher (p less than 0.001, t-test) than in a non-transfected, non-tumorigenic, rat esophageal epithelial cell line. There was a highly variable number of silver grains above individual cells. Significantly fewer silver grains were counted over cells that had been preincubated with either non-labeled v-src or v-Ha-ras DNA or that were pretreated with RNase A. Both oncogene transfected cell lines contained approximately 10 times more oncogene related mRNAs than non-transfected cells as judged by the numbers of silver grains over individual cells. Filter-hybridization analysis of the transfected and non-transfected cell lines confirmed that the expression of src and Ha-ras transcripts was higher in the transfected cell lines than in the non-transfected cell line. Therefore, the in situ hybridization technique would appear useful for the identification of oncogene transcripts in single cells and could potentially be applied to cytological preparations of human cells and to human tumor cells in culture.
Assuntos
Hibridização de Ácido Nucleico , Oncogenes , RNA Mensageiro/genética , Animais , Autorradiografia , Sequência de Bases , Linhagem Celular , Transformação Celular Neoplásica , Transformação Celular Viral , DNA/genética , Humanos , Camundongos , Ratos , Transcrição Gênica , TransfecçãoRESUMO
A quantitative method for the analysis of 201thallium myocardial scintigrams, developed in an experimental infarcted dog heart model, has been compared with two nonquantitative methods for interpretation of stress myocardial scintigrams in two groups of patients studied with coronary angiography: 11 with normal coronary arteries and 14 with coronary artery disease. Three independent observers interpreted scintigrams which were 1) not computer processed; 2) corrected for background activity in lungs and chest wall; and 3) processed by a computer method which uses a uniform threshold of counts determined from the dog model to define perfusion defects. Interobserver variability as well as sensitivity and specificity of detecting coronary disease were examined. In patients with coronary artery disease interobserver variability was improved by using the computer technique: observers agreed as to the existence of a perfusion defect in 93% of the scintigrams as compared to 55% and 81% for the unprocessed and background-subtracted images respectively. No false positive indications of coronary disease were obtained by any of the three techniques. Use of the computer method did not improve the sensitivity of detecting coronary disease, however--71% compared to 64% for unprocessed images and 79% for background-substracted images. The advantages of this quantitative computer method are increased consistency of interpretation and lack of false positive diagnoses of coronary disease. An improved sensitivity of detection may be gained by varying thallium count thresholds according to anatomic location in the heart.
Assuntos
Computadores , Doença das Coronárias/diagnóstico por imagem , Coração/diagnóstico por imagem , Radioisótopos , Tálio , Animais , Cães , Humanos , Infarto do Miocárdio/diagnóstico por imagem , Infarto do Miocárdio/patologia , Miocárdio/patologia , Esforço Físico , CintilografiaRESUMO
An experimental procedure was devised using the pentobarbital-anesthetized dog that could be used for the comprehensive evaluation of the renal effects of chemicals. After IV or renal arterial administration of 0.9% saline solution (vehicle), 12 renal function determinants were continuously monitored for periods of 2 and 6 hours. At the completion of the 2 or 6 hours of study, the kidneys of a number of dogs (usually between 1 and 7) in each vehicle-treated group were subjected to a modification of the intravascular perfusion-of-fixative technique to evaluate the ultrastructural status of the outer cortical, inner cortical, and outer medullary tissue. The remaining dogs (at least 3) in each vehicle-treated group were given a nonnephrotoxic, but maximally effective, diuretic dose of ethacrynic acid, which enabled an assessment of the functional integrity of the thick ascending limb of Henle's loop. Renal function and glomerular and tubular ultrastructure remained stable in the pentobarbital-anesthetized dog for up to 6 hours after administration of vehicle. Sustained infusion of inulin (included in the procedure to estimate glomerular filtration rate) throughout the duration of the experiments, and pentobarbital anesthesia of various durations did not alter the morphologic status of the canine nephron. The procedure used for the renal perfusion of fixative circumvented any manipulation of the kidneys before fixation and allowed for the acquisition of normal (unaltered) appearing tissue from all areas of the kidneys. The responses of pentobarbital-anesthetized dogs to ethacrynic acid administration were similar when given 2 and 6 hours after the vehicle administration.(ABSTRACT TRUNCATED AT 250 WORDS)