Evaluating effects of tetrabromobisphenol A and microplastics on anaerobic granular sludge: Physicochemical properties, microbial metabolism, and underlying mechanisms.

Tetrabromobisphenol A (TBBPA) and microplastics are emerging contaminants of widespread concern. However, little is known about the effects of combined exposure to TBBPA and microplastics on the physicochemical properties and microbial metabolism of anaerobic granular sludge. This study investigated the effects of TBBPA, polystyrene microplastics (PS MP) and polybutylene succinate microplastics (PBS MP) on the physicochemical properties, microbial communities and microbial metabolic levels of anaerobic granular sludge. The results showed that chemical oxygen demand (COD) removal of sludge was lowest in the presence of TBBPA alone and PS MP alone with 33.21% and 30.06%, respectively. The microorganisms promoted the secretion of humic substances under the influence of TBBPA, PS MP and PBS MP. The lowest proportion of genes controlling glycolytic metabolism in sludge was 1.52% when both TBBPA and PS MP were added. Microbial reactive oxygen species were increased in anaerobic granular sludge exposed to MPS. In addition, TBBPA treatment decreased electron transfer of the anaerobic granular sludge and disrupted the pathway of anaerobic microorganisms in acquiring adenosine triphosphate, and MPs attenuated the negative effects of TBBPA on the acetate methanogenesis process of the anaerobic granular sludge. This study provides a reference for evaluating the impact of multiple pollutants on anaerobic granular sludge.

Effects of flotation reagents with aniline aerofloat and ammonium dibutyl dithiophosphate on a constructed rapid infiltration system: Performance and microbial metabolic pathways.

Aniline aerofloat (AAF) and ammonium dibutyl dithiophosphate (ADD) are the key flotation reagents in mineral processing. This study investigated the performance of the constructed rapid infiltration systems with coke and red mud as adsorbents for treatment AAF and ADD wastewater. Meanwhile, the effects of AAF and ADD on the microbial metabolic pathways of the systems were unraveled. Results showed that the AAF concentration in influent was 25 mg/L, which promoted chemical oxygen demand (COD) and total phosphorus (TP) removal of the A column (coke) and B column (red mud). While the COD and TP removal of the C column (coke) and D column (red mud) were inhibited with ADD concentration increasing to 50 mg/L and 100 mg/L. The AAF reduced the binding energy of coke C-O bond by 0.9 eV, and down-regulated the C-C bond ratio by 40.72%. The dominant phyla in the columns were Pseudomonadota and Actinomycetota. The pore structure of coke was more conducive to the growth of the Pseudomonadota, while the metal composition of red mud was more conducive to the redox reaction of microorganisms. The presence of phosphofructokinase (2.7.1.11)-related genes was up-regulated in column C compared to other columns. The ADD was beneficial to the expression of norC and nosZ functional genes during nitrogen metabolism process. In contrast, phosphorus metabolism genes were more expressed in the red mud column for treatment AAF wastewater. This study reveals the potential of coke and red mud for the treatment of flotation reagents wastewater, while providing a theoretical basis for the optimal selection of filler types in the constructed rapid infiltration systems.