The studying of fosinopril combined with fenofibrate on the preventing of diabetic retinopathy in diabetic mice / 天津医药

Objective To study the effect and mechanism of the Fosinopril combined with Fenofibrate on the prevent?ing of diabetic retinopathy. Methods A total of 150 viripotent ICR mice(100 male mice, 50 female mice) were randomly di?vided into five groups(n=30), including A group (Sham group), B group (Model group), C group [Fosinopril prevented group, 20 mg/(kg·d)], D group [Fenofibrate prevented group, 400 mg/(kg·d)] and E group (Fosinopril combined with Fenofibrate pre?vented group). The expression levels of Bax and Bcl-2 gene mRNA were determined by RT-PCR method. TUNEL staining method was used to detect the apoptosisi of retinal cells. Results The Bcl-2 mRNA of A group, Bax mRNA of B group were higher than those of other four groups. Bcl-2 mRNA of E group was higher than that of C group and D group, while the Bax mRNA was lower than those of two groups(all P<0.05). The TUNEL index of B group was the highest than other groups, which of E group

Clinical study of oxidative stress and inflammatory factors in patients with diabetic retinopathy / 中国现代医生

Objective By measuring the level of serum advanced oxidation protein products (AOPP), vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in patients with diabetic retinopathy (DR), and to explore its possible role in the pathogenesis of DR. Methods 90 type 2 diabetes mellitus (T2DM) patients were divided into three groups:30 patients with non-DR (NDR), 30 patients with back ground-DR (BDR), 30 patients with prolifer-ative-DR (PDR). At the same time, 30 healthy controls were selected. The concentration of serum AOPP was measured by modified Wikto-Sarsat, while VEGF and PEDF by ELISA assay. Results The serum level of AOPP increased gradu ally along with the development of DR, and the difference was statistically significant (P<0.05). Serum levels of VEGF in T2DM groups were significantly increased compared with the control group (P<0.05), and VEGF in BDR and PDR groups were significantly higher than that in NDR group (P<0.05), but the serum level of VEGF in PDR group was sig-nificantly lower than that in BDR group (P<0.05). The serum level of PEDF in each group was gradually decreased, and the difference was statistically significant (P<0.05). Pearson correlation analysis showed that the serum level of VEGF was negatively correlated with PEDF(r=-0.734, P<0.05). The serum level of AOPP was positively correlated with VEGF (r=0.816, P<0.05), and negatively correlated with PEDF (r=-0.905, P<0.05). Conclusion The increase of AOPP and VEGF level in serum of patients with diabetes, and the decrease of PEDF may promote the development of DR.