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1.
Int J Impot Res ; 18(5): 432-7, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16528291

RESUMO

The aim was to determine in circulating mononuclear cells from patients with erectile dysfunction (ED), the level of expression of endothelial nitric oxide synthase (eNOS), soluble guanylate cyclase (sGC) beta1-subunit and phosphodiesterase type-V (PDE-V). Peripheral mononuclear cells from nine patients with ED of vascular origin and nine patients with ED of neurological origin were obtained. Fourteen age-matched volunteers with normal erectile function were used as control. Reduction in eNOS protein was observed in the mononuclear cells from patients with ED of vascular origin but not in those from neurological origin. Although sGC beta1-subunit expression was increased in mononuclear cells from patients with ED, the sGC activity was reduced. However, only the patients with ED of vascular origin showed an increased expression of PDE-V. This work shows for the first time that, independently of the aetiology of ED, the expression of sGC beta1-subunit was increased in circulating mononuclear cells; however, the expression of both eNOS and PDE-V was only modified in the circulating mononuclear cells from patients with ED of vascular origin.


Assuntos
Disfunção Erétil/enzimologia , Guanilato Ciclase/metabolismo , Leucócitos/enzimologia , Regulação para Cima , 3',5'-GMP Cíclico Fosfodiesterases/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , GMP Cíclico/biossíntese , Nucleotídeo Cíclico Fosfodiesterase do Tipo 5 , Humanos , Masculino , Pessoa de Meia-Idade , Óxido Nítrico Sintase Tipo III/metabolismo , Subunidades Proteicas/metabolismo , Solubilidade
8.
Arch Esp Urol ; 43 Suppl 1: 35-47, 1990.
Artigo em Espanhol | MEDLINE | ID: mdl-2078067

RESUMO

We evaluated 137 patients with bladder cancer submitted to surgery in order to determine the usefulness of intravesical ultrasonography (IVU) in tumor staging. The characteristic scan images were defined for each tumor stage. The application of the ultrasound patterns yielded an accuracy rate of 91% for tumors Ta"1 and 71% for T4. Based on the results of this study, IVU does not appear to be useful as an intraoperative monitoring technique.


Assuntos
Neoplasias da Bexiga Urinária/diagnóstico por imagem , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Monitorização Intraoperatória , Análise Multivariada , Invasividade Neoplásica , Estadiamento de Neoplasias/métodos , Ultrassonografia , Neoplasias da Bexiga Urinária/patologia , Neoplasias da Bexiga Urinária/cirurgia
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