RESUMO
Reversible pressure-induced disassembly of several viruses has suggested the idea of using hydrostatic pressure to suppress virus infectivity. In this study, the effects of high hydrostatic pressure and ultraviolet (UV) irradiation were investigated on classical swine fever virus (CSFV) in an attempt to eliminate residual infectivity. The structural modifications were followed by intrinsic fluorescence and biological activity assays. The kinetics of CSFV inactivation showed that pressure-induced inactivation was not enough to eliminate viral infectivity. However, when pressure was applied in association with UV irradiation no infectious focus was observed. The application of these two methods against CSFV can be an attractive inactivation strategy for the development of a vaccine.
Assuntos
Vírus da Febre Suína Clássica/efeitos da radiação , Animais , Linhagem Celular , Peste Suína Clássica/prevenção & controle , Vírus da Febre Suína Clássica/isolamento & purificação , Vírus da Febre Suína Clássica/patogenicidade , Pressão Hidrostática , Técnicas In Vitro , Cinética , Luz , Espalhamento de Radiação , Espectrometria de Fluorescência , Sus scrofa , Raios Ultravioleta , Vacinas Atenuadas/isolamento & purificação , Vacinas Virais/isolamento & purificação , Inativação de Vírus/efeitos da radiaçãoRESUMO
Prostaglandins (Pgs) have been shown to inhibit the replication of several DNA and RNA viruses. Here we report the effect of prostaglandin (PgA1) on the multiplication of a positive strand RNA virus, Classical Swine Fever Virus (CSFV) in PK15 cells. PgA1 was found to inhibit the multiplication of CSFV. At a concentration of 5 micrograms/ml, which was nontoxic to the cells, PgA1 inhibitis virus production in 99%. In PgA1 treated cells the size and number of characteristic Classical Swine Fever focus decreased in amount.