Cortical Flow-Driven Shapes of Nonadherent Cells.

Nonadherent polarized cells have been observed to have a pearlike, elongated shape. Using a minimal model that describes the cell cortex as a thin layer of contractile active gel, we show that the anisotropy of active stresses, controlled by cortical viscosity and filament ordering, can account for this morphology. The predicted shapes can be determined from the flow pattern only; they prove to be independent of the mechanism at the origin of the cortical flow, and are only weakly sensitive to the cytoplasmic rheology. In the case of actin flows resulting from a contractile instability, we propose a phase diagram of three-dimensional cell shapes that encompasses nonpolarized spherical, elongated, as well as oblate shapes, all of which have been observed in experiment.

Self-similar curling of a naturally curved elastica.

We consider the curling of an initially flat but naturally curved elastica on a hard, nonadhesive surface. Combining theory, simulations, and experiments, we find novel behavior, including a constant front velocity and a self-similar shape of the curl that scales in size as t(1/3) at long times after the release of one end of the elastica. The front velocity is selected by matching the self-similar solution with a roll of nearly constant curvature located near the free end.

Nematic cells with defect-patterned alignment layers.

Using Monte Carlo simulations of the Lebwohl-Lasher model we study the director ordering in a nematic cell where the top and bottom surfaces are patterned with a lattice of +/-1 point topological defects of lattice spacing a . As expected on general physical grounds we find that the nematic order depends on the ratio of the height of the cell H to a . For thick cells (Ha > or = 0.9) we find that the system is very well ordered and the frustration induced by the lattice of defects is relieved in a novel way by a network of half-integer defect lines which emerge from the point defects and hug the top and bottom surfaces of the cell. When Ha < or = 0.9 the system has zero nematic order parameter and the half-integer defect lines thread through the cell joining point defects on the top and bottom surfaces. We present a simple physical argument in terms of the length of the defect lines to explain these results. To facilitate eventual comparison with experimental systems we also simulate optical textures in the presence of crossed polarizers.

Unwinding of a strained cholesteric elastomer by disclination loop nucleation.

The application of a sufficiently strong strain perpendicular to the pitch axis of a monodomain cholesteric elastomer unwinds the cholesteric helix. Previous theoretical analyses of this transition ignored the effects of Frank elasticity which we include here. We find that the strain needed to unwind the helix is reduced because of the Frank penalty and the cholesteric state becomes metastable above the transition. We consider in detail a previously proposed mechanism by which the topologically stable helical texture is removed in the metastable state: namely, by the nucleation of twist disclination loops in the plane perpendicular to the pitch axis. We present an approximate calculation of the barrier energy for this nucleation process which neglects possible spatial variation of the strain fields in the elastomer, as well as a more accurate calculation based on a finite-element modeling of the elastomer.

Simulation and visualization of topological defects in nematic liquid crystals.

We present a method of visualizing topological defects arising in numerical simulations of liquid crystals. The method is based on scientific visualization techniques developed to visualize second-rank tensor fields, yielding information not only on the local structure of the field but also on the continuity of these structures. We show how these techniques can be used to first locate topological defects in fluid simulations of nematic liquid crystals where the locations are not known a priori and then study the properties of these defects including the core structure. We apply these techniques to simulation data obtained by previous authors who studied a rapid quench and subsequent equilibration of a Gay-Berne nematic. The quench produces a large number of disclination loops which we locate and track with the visualization methods. We show that the cores of the disclination lines have a biaxial region and the loops themselves are of a hybrid wedge-twist variety.

Viscous-fingering-like instability of cell fragments.

We present a novel flow instability that can arise in thin films of cytoskeletal fluids if the friction with the substrate on which the film lies is sufficiently strong. We consider a two-dimensional, membrane-bound fragment containing actin filaments that polymerize at the edge and depolymerize in the fragment. Performing a linear stability analysis of the initial state due to perturbations of the fragment boundary, we find, in the limit of large friction, that the perturbed actin velocity and pressure fields obey the same laws governing the viscous fingering instability of an interface between immiscible fluids in a Hele-Shaw cell. A remarkable feature of this instability is that it is independent of the strength of the interaction between actin filaments and myosin motors.