High Value-Added Reutilization of Waste-Printed Circuit Boards Non-Metallic Components in Sustainable Polymer Composites.

The reutilization non-metallic components from a waste-printed circuit board (WPCB) has become one of the most significant bottlenecks in the comprehensive reuse of electronic wastes due to its low value and complex compositions, and it has received great attention from scientific and industrial researchers. To effectively address the environmental pollution caused by inappropriate recycling methods, such as incineration and landfill, extensive efforts have been dedicated to achieving the high value-added reutilization of WPCB non-metals in sustainable polymer composites. In this review, recent progress in developing sustainable polymer composites based on WPCB non-metallic components was systematically summarized. It has been demonstrated that the WPCB non-metals can serve as a promising reinforcing and functional fillers to significantly ameliorate some of the physical and chemical properties of polymer composites, such as excellent mechanical properties, enhanced thermal stability, and flame retardancy. The recovery strategies and composition of WPCB non-metals were also briefly discussed. Finally, the future potentials and remaining challenges regarding the reutilization of WPCB non-metallic components are outlined. This work provides readers with a comprehensive understanding of the preparation, structure, and properties of the polymer composites based on WPCB non-metals, providing significant insights regarding the high value-added reutilization of WPCB non-metals of electronic wastes.

Involvement of ectodomain Leu 214 in ATP binding and channel desensitization of the P2X4 receptor.

P2X receptors are trimeric ATP-gated cation permeable ion channels. When ATP binds, the extracellular head and dorsal fin domains are predicted to move closer to each other. However, there are scant functional data corroborating the role of the dorsal fin in ligand binding. Here using site-directed mutagenesis and electrophysiology, we show that a dorsal fin leucine, L214, contributes to ATP binding. Mutant receptors containing a single substitution of alanine, serine, glutamic acid, or phenylalanine at L214 of the rat P2X4 receptor exhibited markedly reduced sensitivities to ATP. Mutation of other dorsal fin side chains, S216, T223, and D224, did not significantly alter ATP sensitivity. Exposure of L214C to sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES(-)) or (2-aminoethyl) methanethiosulfonate hydrobromide in the absence of ATP blocked responses evoked by subsequent ATP application. In contrast, when MTSES(-) was applied in the presence of ATP, no current inhibition was observed. Furthermore, L214A also slightly reduced the inhibitory effect of the antagonist 2',3'-O-(2,4,6-trinitrophenyl)-ATP, and the blockade was more rapidly reversible after washout. Certain L214 mutants also showed effects on current desensitization in the continued presence of ATP. L214I exhibited an accelerated current decline, whereas L214M exhibited a slower rate. Taken together, these data reveal that position L214 participates in both ATP binding and conformational changes accompanying channel opening and desensitization, providing compelling evidence that the dorsal fin domain indeed has functional properties that are similar to those previously reported for the body domains.

Effect and mechanism of polysilazane and platinum on tracking and erosion resistance of silicone rubber.

Polysilazane (PSiN) and platinum (Pt) were used to enhance the tracking and erosion resistance of silicone rubber. The suppression effects of PSiN and Pt on tracking and erosion were investigated using an inclined plane test (IPT), thermogravimetry, thermogravimetry-Fourier transform infrared spectrometry, scanning electron microscopy and laser Raman spectroscopy. It was determined that the addition of 1.8-2.4 part per hundred parts of rubber (phr) of PSiN and 8 ppm of Pt significantly enhanced the tracking and erosion resistance of silicone rubber, whereby the test specimens passed a 4.5 kV IPT with a low eroded mass and undamaged surfaces. This was attributed to the synergistic effect of PSiN and Pt on silicone chains. At high temperatures and produced by arc discharge, PSiN/Pt-catalysed radical crosslinking suppressed the degradation of silicone chains. Furthermore, the formation of a tightly crosslinked network protected the inner materials from arc ablation. In addition, carbon deposition was prevented by PSiN/Pt, making it harder for tracking to develop.

Phase Change Composite Microcapsules with Low-Dimensional Thermally Conductive Nanofillers: Preparation, Performance, and Applications.

Phase change materials (PCMs) have been extensively utilized in latent thermal energy storage (TES) and thermal management systems to bridge the gap between thermal energy supply and demand in time and space, which have received unprecedented attention in the past few years. To effectively address the undesirable inherent defects of pristine PCMs such as leakage, low thermal conductivity, supercooling, and corrosion, enormous efforts have been dedicated to developing various advanced microencapsulated PCMs (MEPCMs). In particular, the low-dimensional thermally conductive nanofillers with tailorable properties promise numerous opportunities for the preparation of high-performance MEPCMs. In this review, recent advances in this field are systematically summarized to deliver the readers a comprehensive understanding of the significant influence of low-dimensional nanofillers on the properties of various MEPCMs and thus provide meaningful enlightenment for the rational design and multifunction of advanced MEPCMs. The composition and preparation strategies of MEPCMs as well as their thermal management applications are also discussed. Finally, the future perspectives and challenges of low-dimensional thermally conductive nanofillers for constructing high performance MEPCMs are outlined.

Targeted demethylation at ZNF154 promotor upregulates ZNF154 expression and inhibits the proliferation and migration of Esophageal Squamous Carcinoma cells.

Zinc finger protein 154 (ZNF154) is hypermethylated at the promoter in many epithelial-derived solid tumors. However, its methylation status and function in esophageal squamous carcinoma (ESCC) are poorly understood. We found that the ZNF154 promoter is hypermethylated in ESCC and portends poor prognosis. In addition, ZNF154 functions as a tumor suppressor gene (TSG) in ESCC, and is downregulated by promoter hypermethylation. We established a targeted demethylation strategy based on CRISPR/dCas9 technology and found that the hypermethylation of ZNF154 promoter repressed ZNF154 induction, which in turn promoted the proliferation and migration of ESCC cells in vitro and in vivo. Finally, high-throughput CUT&Tag analysis, GEPIA software and qPCR were used to revealed the role of ZNF154 as a transcription factor to upregulate the expression of ESCC-associated tumor suppressor genes. Taken together, hypermethylation of the ZNF154 promoter plays an important role in the development of ESCC, and epigenetic editing is a promising tool for inhibiting ESCC cells with aberrant DNA methylation.

Immobilization of rubber additive on graphene for high-performance rubber composites.

It is still a challenge to achieve simultaneous improvements in aging resistance, mechanical strength, thermal conductivity and dielectric constant of rubber composites via incorporation of graphene obtained by conventional methods. Herein, an effective and green method was proposed to simultaneously reduce and functionalize graphene oxide (GO) with 2-mercaptobenzimidazole (antioxidant MB) via a one-pot method. GO was successfully reduced by MB which was also chemically grafted on the reduced GO (G-MB). G-MB sheets were uniformly dispersed in rubber with strong interfacial interaction, and graphene-graphene conductive paths were formed through intermolecular H-bonding between the grafted antioxidant molecules. Consequently, rubber composites with G-MB showed higher thermal conductivity, mechanical strength and dielectric constant than rubber composites with hydrazine hydrate reduced GO (rGO). Moreover, the thermo-oxidative aging resistance of rubber composites with G-MB was also superior to that of rubber composites with rGO because of the elimination of blooming effect of the grafted MB molecules. Thus, this work may open a new way for the eco-friendly functionalization and reduction of GO and may boost the development of high-performance, functional graphene-elastomer composites.

Preparation, characterization and application of rod-like chitin nanocrystal by using p-toluenesulfonic acid/choline chloride deep eutectic solvent as a hydrolytic media.

Chitin nanocrystal (ChiNC) was fabricated based on p-toluenesulfonic acid -choline chloride deep eutectic solvent treatment. The obtained ChiNC was about 12-44 nm in width and 206-399 nm in length. The crystalline structure and the functional groups of ChiNC were maintained during the preparation process. Moreover, porcine pancreas lipase (PPL) was successfully immobilized onto the ChiNC to form the immobilized PPL (PPL@ChiNC). The resulting PPL@ChiNC has enzyme loading and activity recovery of 35.6 mg/g and 82.5%, respectively. The thermal stability, pH and temperature adaptabilities of PPL@ChiNC was improved, comparing with free PPL. The demonstrated DES treatment process was efficient for ChiNC preparation and the as-prepared ChiNC exhibited great potentials in biocatalysis and biomedical field.

Inhibition of HERG potassium channels by domiphen bromide and didecyl dimethylammonium bromide.

Domiphen bromide and didecyl dimethylammonium bromide were widely used environmental chemicals with potent activity on blockade of human ether-a-go-go related gene (HERG) channels. But the mechanism of their action is not clear. The kinetics of block of HERG channels by domiphen bromide and didecyl dimethylammonium bromide was studied in order to characterize the inhibition of HERG currents by these quaternary ammonium compounds (QACs). Domiphen bromide and didecyl dimethylammonium bromide inhibited HERG channel currents in a dose-dependent manner with IC50 values of 9nM and 5nM, respectively. Block of HERG channel by domiphen bromide and didecyl dimethylammonium bromide was voltage-dependent and use-dependent. Domiphen bromide and didecyl dimethylammonium bromide caused substantial negative shift of the activation curves, accelerated activated process, but had no effects on the deactivation and reactivation processes. The docking models implied that these two compounds bound to PAS domain of HERG channels and inhibited its function. Our data demonstrated that domiphen bromide and didecyl dimethylammonium bromide blocked the HERG channel with a preference for the activated channel state.

Generation of the SCN1A epilepsy mutation in hiPS cells using the TALEN technique.

Human induced pluripotent stem cells (iPSC) can be used to understand the pathological mechanisms of human disease. These cells are a promising source for cell-replacement therapy. However, such studies require genetically defined conditions. Such genetic manipulations can be performed using the novel Transcription Activator-Like Effector Nucleases (TALENs), which generate site-specific double-strand DNA breaks (DSBs) with high efficiency and precision. Combining the TALEN and iPSC methods, we developed two iPS cell lines by generating the point mutation A5768G in the SCN1A gene, which encodes the voltage-gated sodium channel Nav1.1 α subunit. The engineered iPSC maintained pluripotency and successfully differentiated into neurons with normal functional characteristics. The two cell lines differ exclusively at the epilepsy-susceptibility variant. The ability to robustly introduce disease-causing point mutations in normal hiPS cell lines can be used to generate a human cell model for studying epileptic mechanisms and for drug screening.