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Acrylamide (AAM), a compound extensively utilized in various industrial applications, has been reported to induce toxic effects across multiple tissues in living organisms. Despite its widespread use, the impact of AAM on ovarian function and the mechanisms underlying these effects remain poorly understood. Here, we established an AAM-exposed mouse toxicological model using 21 days of intragastric AAM administration. AAM exposure decreased ovarian coefficient and impaired follicle development. Further investigations revealed AAM would trigger apoptosis and disturb tricarboxylic acid cycle in ovarian tissue, thus affecting mitochondrial electron transport function. Moreover, AAM exposure decreased oocyte and embryo development potential, mechanically associated with pericentrin and phosphorylated Aurora A cluster failure, leading to meiotic spindle assembly defects. Collectively, these results suggest that AAM exposure may lead to apoptosis, glucose metabolic disorders, and mitochondrial dysfunction in ovary tissue, ultimately compromising oocyte quality.
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With the rapid development of various industries, cyanide (CN-) and hypochlorite (ClO-) have a tremendously adverse effect on the health of humans and animals. In this study, a fluorescent probe HHTB based on a benzaldehyde-indole fused chromophore was designed to detect cyanide and hypochlorite simultaneously. The synthesized probe was found to have strong anti-interference ability. In addition, the designed probe could respond rapidly to ClO- in just 80 s, while the color changed visibly from red to colorless. Moreover, the response time to CN- was longer (about 160 s), with the apparent color change from red to light red. The ratiometric and colorimetric absorbance variation of HHTB was due to the nucleophilic attack of CN- on the indole CîN functional group and the strong oxidization of ClO- which destroyed the CîC bonds and the conjugation systems. Furthermore, the probe HHTB responding to ClO- and CN- presented high sensitivity, as the calculated detection limits were 1.18 nM and 1.40 nM, respectively. The probe was also found to have low biological toxicity and was used in living cells successfully. Therefore, it has good application prospect in the field of cell imaging and biomedicine. The binding mechanism of HHTB-CN and the reaction mechanism of HHTB and ClO- were further elucidated by a series of experiments.
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Corantes Fluorescentes , Ácido Hipocloroso , Animais , Benzaldeídos/toxicidade , Cianetos/toxicidade , Corantes Fluorescentes/toxicidade , Humanos , Ácido Hipocloroso/toxicidade , Indóis/toxicidadeRESUMO
Hypochlorite (ClO-) is an important reactive oxygen species (ROS) in organisms. In this work, a fluorescent probe DBTM based on triphenylamine was synthesized successfully and characterized by spectral methods. The designed probe can rapidly respond to ClO- in just 1 min, followed by the apparent color change from red to yellow. The colorimetric and ratiometric absorbance change of DBTM was attributed to the strong oxidation of ClO-, which broke the connected double bonds and destroyed the conjugate system. The probe DBTM showed an excellent selectivity towards ClO- in comparison with other ROS probes. Besides, the DBTM probe exhibited a highly sensitive response to ClO-, with the detection limits calculated to be 3.3 nM. The probe can be applied in the form of cotton swabs and test strips that could detect ClO- easily, suggesting its potential use as imaging agents for realistic ClO- detection. In particular, DBTM exhibited very low background fluorescence in living cells and was able to detect the minor variation of endogenous hypochlorite in L929 cells. Based on these advantages, the probe DBTM could be a good candidate for detecting ClO- in biological systems.
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Compostos de Anilina/química , Corantes Fluorescentes/química , Ácido Hipocloroso/análise , Animais , Ânions/análise , Linhagem Celular , Camundongos , Microscopia de Fluorescência/métodos , Modelos Moleculares , Imagem Óptica/métodos , Espectrometria de Fluorescência/métodosRESUMO
Hypochlorite (ClO- ), as one of the active oxygen species (ROS), plays an essential role in the cellular defence system and organism immunity. In this paper, we successfully synthesized a new 'turn-on' fluorescent probe BMF based on benzimidazole and characterized it by spectroscopic methods. The designed probe can quickly respond to ClO- with the obvious colour change from pink to colourless. Notably, the probe BMF exhibited almost no fluorescence, but showed strong fluorescence after adding ClO- , including an excellent fluorescence turn-on effect. The fluorescence turn-on phenomenon of BMF was attributed to the strong oxidation of ClO- , which severed the connecting double bond and disrupted the intramolecular charge transfer (ICT) system, plus light-induced electron transfer effect between the fluorophore and the recognition group was discontinued. In addition, the cytotoxicity assay showed that the probe had lower cytotoxicity. Based on these advantages, we demonstrated that probe BMF might be a good candidate for detecting ClO- in biological systems.
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Corantes Fluorescentes , Ácido Hipocloroso , Benzimidazóis , Fluorescência , ÍonsRESUMO
Two simple and novel fluorescent probes (CDC1 and CDC2) have been designed and prepared here for sensing HSO3- with large Stokes shifts (about 250 nm). The synthesized probes can react with HSO3- just in 2 min, followed by the obvious color change from blue to colorless. The colorimetric and ratiometric absorbance response of the probes to HSO3- is due to the addition of HSO3- to the electron-deficient C=C double bond group, which prevents significant intramolecular charge transfer (ICT). Besides, CDC1 and CDC2 can detect HSO3- in pure water and detection limits of CDC1 and CDC2 reached 4.59 nM and 8.19 nM, respectively. Considering the delicate difference in the two prepared probes' molecular structures, CDC1 containing the carboxyl group has a more significant fluorescence intensity change response to HSO3- in pure water than CDC2 (with sulfinyl group). Beyond better response characteristics, CDC1 also has lower cytotoxicity and better biocompatibility compared with CDC2, which could be chosen to detect HSO3- in living cells. With these superior properties, probe CDC1 could have a potential application in the fields of environmental and biological detection.
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Acroleína/análogos & derivados , Corantes Fluorescentes/química , Sulfitos/química , Água/química , Acroleína/química , Animais , Linhagem Celular Tumoral , Humanos , Limite de Detecção , Camundongos , Espectrofotometria UltravioletaRESUMO
With the rapid development of the Internet of Things (IoTs), wireless sensor networks (WSNs) and related techniques, the amount of sensory data manifests an explosive growth. In some applications of IoTs and WSNs, the size of sensory data has already exceeded several petabytes annually, which brings too many troubles and challenges for the data collection, which is a primary operation in IoTs and WSNs. Since the exact data collection is not affordable for many WSN and IoT systems due to the limitations on bandwidth and energy, many approximate data collection algorithms have been proposed in the last decade. This survey reviews the state of the art of approximatedatacollectionalgorithms. Weclassifythemintothreecategories: themodel-basedones, the compressive sensing based ones, and the query-driven ones. For each category of algorithms, the advantages and disadvantages are elaborated, some challenges and unsolved problems are pointed out, and the research prospects are forecasted.
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Multicasting is a fundamental network service for one-to-many communications in wireless sensor networks. However, when the sensor nodes work in an asynchronous duty-cycled way, the sender may need to transmit the same message several times to one group of its neighboring nodes, which complicates the minimum energy multicasting problem. Thus, in this paper, we study the problem of minimum energy multicasting with adjusted power (the MEMAP problem) in the duty-cycled sensor networks, and we prove it to be NP-hard. To solve such a problem, the concept of an auxiliary graph is proposed to integrate the scheduling problem of the transmitting power and transmitting time slot and the constructing problem of the minimum multicast tree in MEMAP, and a greedy algorithm is proposed to construct such a graph. Based on the proposed auxiliary graph, an approximate scheduling and constructing algorithm with an approximation ratio of 4 l n K is proposed, where K is the number of destination nodes. Finally, the theoretical analysis and experimental results verify the efficiency of the proposed algorithm in terms of the energy cost and transmission redundancy.
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Various water pollution issues pose a significant threat to human water safety. Magnetic polydopamine composites (MPCs), which can be separated by magnetic fields after the adsorption process, exhibit outstanding adsorption capacity and heterogeneous catalytic properties, making them promising materials for water treatment applications. In particular, by modifying the polydopamine (PDA) coating, MPCs can acquire enhanced high reactivity, antibacterial properties, and biocompatibility. This also provides an attractive platform for further fabrication of hybrid materials with specific adsorption, catalytic, antibacterial, and water-oil separation capabilities. To systematically provide the background knowledge and recent research advances in MPCs, this paper presents a critical review of MPCs for water treatment in terms of both structure and mechanisms of effect in applications. Firstly, the impact of different PDA positions within the composite structure is investigated to summarize the optimization of properties contributed by PDA when acting as the shell, core, or bridge. The roles of various secondary modifications of magnetic materials by PDA in addressing water pollution problems are explored. It is anticipated that this work will be a stimulus for further research and development of magnetic composite materials with real-world application potential.
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Indóis , Polímeros , Purificação da Água , Humanos , Polímeros/química , Antibacterianos , Fenômenos MagnéticosRESUMO
Acrolein is a widespread contaminant found in both diet and environment, entering the human body through food, alcohol, smoking, and exposure to fuel combustion fumes. While prior studies have highlighted acrolein's harmful impact on oocyte quality and early embryonic development in vitro, the specific mechanisms by which acrolein affects the female reproductive system in vivo remain poorly understood. This study first confirmed that in vitro acrolein exposure disrupts spindle morphology and chromosome alignment during the mid-MI stage of oocyte development, thus hindering oocyte maturation. Besides, exposure to acrolein not only stunts growth in mice but also impairs ovarian development, decreases the ovarian coefficient, disrupts follicular development, and increases the count of atretic follicles in vivo. Additional research has shown that acrolein exposure reduces the activity of key enzymes in glycolysis, pyruvate metabolism, and the tricarboxylic acid cycle within the ovaries. It also suppresses mitochondrial complex expression and disturbs the balance between mitochondrial fission and fusion, as confirmed by metabolomic analyses. Moreover, acrolein exposure in vivo induced granulosa cell apoptosis and reduced oocyte number. In summary, acrolein exposure impairs glucose metabolism and induces mitochondrial dysfunction in the ovaries.
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Atrazine is a widely used herbicide in agricultural production. Previous studies have shown that atrazine affects hormone secretion and oocyte maturation in female reproduction. However, the specific mechanism by which atrazine affects ovarian function remains unclear. In this study, using a mouse gastric lavage model, we report that four weeks of atrazine exposure affects body growth, interferes with the estrous cycle, and increases the number of atretic follicles in mice. The expression levels of follicle development related factors StAR, BMP15, and AMH decreased. Metabolomic analysis revealed that atrazine activates an inflammatory response in ovarian tissue. Further studies confirmed that the expression levels of TNF-α, IL-6, and NF-κB increased in the ovaries of mice exposed to atrazine. Additionally, α-smooth muscle actin (α-SMA) accumulated in ovarian tissue, and transforming growth factor-ß (TGF-ß) signaling was activated, indicating the occurrence of tissue fibrosis. Moreover, mice exposed to atrazine produced fewer oocytes and exhibited reduced embryonic development. Furthermore, mice exposed to atrazine exhibited altered gut microbiota abundance and a disrupted colon barrier. Collectively, these findings suggest that atrazine exposure induces ovarian inflammation and fibrosis, disrupts ovarian homeostasis, and impairs follicle maturation, ultimately reducing oocyte quality.
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ETHNOPHARMACOLOGICAL RELEVANCE: Hypericum perforatum L. (genus Hypericum, family Hypericaceae) is a flowering plant native to Europe, North Africa and Asia, which can be used in the treatment of psychiatric disorder, cardiothoracic depression and diabetes. Crataegus pinnatifida Bunge (genus Crataegus pinnatifida Bunge, family Rosaceae) was another traditional Chinese medicine for treating hyperlipidemia. Hyperoside (Hype), a major flavonoid glycoside component of Hypericum perforatum L. and Crataegus pinnatifida Bunge, possesses multiple physiological activities, such as anti-inflammatory and antioxidant effects. However, the role of Hype on obesity and related metabolic diseases still needs to be further investigated. AIM OF THE STUDY: We explored the effect of Hype on high-fat diet (HFD)-induced obesity and its metabolic regulation on white fat tissues. MATERIALS AND METHODS: In vivo four-week-old male C57BL/6J mice were randomly assigned to vehicle (0.5% methycellulose) and Hype (80 mg/kg/day by gavage) group under a normal chow diet (NCD) or HFD for 8 weeks. In vitro, 3T3-L1 preadipocyte cell line and primary stromal vascular fraction (SVF) cells from inguinal white adipose tissue (iWAT) of mice were used to investigate the molecular mechanisms of Hype regulation on adipocyte energy metabolism. RESULTS: Hype treatment in vivo promotes UCP1-dependent white to beige fat transition, increases glucose and lipid metabolism, and resists HFD-induced obesity. Meanwhile, Hype induces lipophagy, a specific autophagy that facilitates the breakdown of lipid droplets, and blocking autophagy partially reduces UCP1 expression. Mechanistically, Hype inhibited CDK6, leading to the increased nuclear translocation of TFEB, while overexpression of CDK6 partially reversed the enhancement of UCP1 by Hype. CONCLUSIONS: Hype protects mice from HFD-induced obesity by increasing energy expenditure of white fat tissue via CDK6-TFEB pathway.
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Dieta Hiperlipídica , Obesidade , Animais , Camundongos , Tecido Adiposo Branco , Autofagia , Camundongos Endogâmicos C57BL , Obesidade/tratamento farmacológico , TermogêneseRESUMO
Activation of adipose tissue thermogenesis is a promising strategy in the treatment of obesity and obesity-related metabolic disorders. Kaempferol (KPF) is a predominant dietary flavonoid with multiple pharmacological properties, such as anti-inflammatory and antioxidant activities. In this study, we sought to characterize the role of KPF in adipocyte thermogenesis. We demonstrated that KPF-treated mice were protected from diet-induced obesity, glucose tolerance, and insulin resistance, accompanied by markedly increased energy expenditure, ex vivo oxygen consumption of white fat, and increased expression of proteins related to adaptive thermogenesis. KPF-promoted beige cell formation is a cell-autonomous effect, since the overexpression of cyclin-dependent kinase 6 (CDK6) in preadipocytes partially reversed browning phenotypes observed in KPF-treated cells. Overall, these data implicate that KPF is involved in promoting beige cell formation by suppressing CDK6 protein expression. This study provides evidence that KPF is a promising natural product for obesity treatment by boosting energy expenditure.
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Subunidade alfa 2 de Fator de Ligação ao Core , Quinase 6 Dependente de Ciclina , Animais , Camundongos , Quinase 6 Dependente de Ciclina/metabolismo , Quinase 6 Dependente de Ciclina/farmacologia , Quinase 6 Dependente de Ciclina/uso terapêutico , Subunidade alfa 2 de Fator de Ligação ao Core/metabolismo , Subunidade alfa 2 de Fator de Ligação ao Core/farmacologia , Subunidade alfa 2 de Fator de Ligação ao Core/uso terapêutico , Tecido Adiposo Marrom/metabolismo , Quempferóis/farmacologia , Adipócitos , Obesidade/tratamento farmacológico , Obesidade/genética , Obesidade/metabolismo , Tecido Adiposo Branco/metabolismo , Dieta Hiperlipídica/efeitos adversos , Transdução de Sinais , Termogênese , Camundongos Endogâmicos C57BL , Metabolismo EnergéticoRESUMO
Adipocyte browning increases energy expenditure by thermogenesis, which has been considered a potential strategy against obesity and its related metabolic diseases. Phytochemicals derived from natural products with the ability to improve adipocyte thermogenesis have aroused extensive attention. Acteoside (Act), a phenylethanoid glycoside, exists in various medicinal or edible plants and has been shown to regulate metabolic disorders. Here, the browning effect of Act was evaluated by stimulating beige cell differentiation from the stromal vascular fraction (SVF) in the inguinal white adipose tissue (iWAT) and 3T3-L1 preadipocytes, and by converting the iWAT-SVF derived mature white adipocytes. Act improves adipocyte browning by differentiation of the stem/progenitors into beige cells and by direct conversion of mature white adipocytes into beige cells. Mechanistically, Act inhibited CDK6 and mTOR, and consequently relieved phosphorylation of the transcription factor EB (TFEB) and increased its nuclear retention, leading to induction of PGC-1α, a driver of mitochondrial biogenesis, and UCP1-dependent browning. These data thus unveil a CDK6-mTORC1-TFEB pathway that regulates Act-induced adipocyte browning.
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Tecido Adiposo Branco , Doenças Metabólicas , Humanos , Alvo Mecanístico do Complexo 1 de Rapamicina/metabolismo , Tecido Adiposo Branco/metabolismo , Obesidade/tratamento farmacológico , Obesidade/metabolismo , Adipócitos Brancos/metabolismo , Doenças Metabólicas/metabolismo , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/metabolismo , Quinase 6 Dependente de Ciclina/metabolismo , Quinase 6 Dependente de Ciclina/farmacologiaRESUMO
The conversion of hexavalent chromium (Cr(VI)), a highly poisonous heavy metal found in natural environment, to less poisonous trivalent chromium (Cr(III)) has attracted a lot of interest. However, little interest has been paid to the development of metal-free catalysts. Here, we demonstrate for the first time a molecular engineering strategy to synthesize a range of donor-acceptor conjugated polymer photocatalysts, which can significantly increase the reduction efficiency of Cr(VI) by a factor of 5.2, corresponding to a significant change in the reduction reaction rate constant (from 0.0337 to 0.1740 min-1). In addition, the apparent quantum efficiency (AQE) of Cr(VI) removal was obtained, and the optimized photocatalyst (Py-SO1) could achieve the highest apparent quantum efficiency at wavelength of 420 nm in those samples. Despite the narrow light absorption of Py-SO1 polymer, its excellent exciton separation efficiency and efficient electron output enabled it to achieve excellent performance in photoreduction of Cr(VI), surpassing that of the reported metal-free photocatalysts. The results show that the present work provides a new perspective for designing suitable environmental remediation catalysts based on molecular engineering strategies.
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Magnetic nanoparticle materials which could be used to remove tetracycline were confined seriously due to their poor stability and unsatisfactory reusability. Here, we facilely prepared novel zeolitic imidazolate framework-8 (ZIF-8) functionalized magnetic nanoparticles (Fe3O4@PDA-ZIF-8) adsorbent utilizing polydopamine as a bond to establish a connection between zeolitic imidazolate framework-8 and Fe3O4, which could improve the stability of magnetic nanoparticles and enhance the tetracycline adsorption capacity simultaneously. The prepared nanocomposites were characterized and their TC adsorption abilities under various experiment conditions (contact time, TC initial concentration and pH values) were also investigated. Experimental results proved that the prepared adsorbent showed superior TC adsorption capacities (92.01 mg/g at pH = 7). Further, the adsorption mechanisms were comprehensively studied and the prepared adsorbent showed satisfactory stability and reusability during the cycle experiment. Altogether, our findings provided a feasible way to design and construct functional magnetic MOF materials for enhancing tetracycline adsorption from wastewater.
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Nanopartículas de Magnetita , Poluentes Químicos da Água , Zeolitas , Adsorção , Antibacterianos/química , Indóis , Polímeros , Tetraciclina/química , Águas Residuárias/química , Poluentes Químicos da Água/análise , Zeolitas/químicaRESUMO
Fenton-like catalysts represent a family of promising materials to degrade micropollutants from contaminated water. However, the practical applications of Fenton-like catalysts are mainly limited by low catalytic degradation efficiency and stability. Herein, for the first time, rapid fabrication of Ag-decorated Fe3O4/polydopamine (FPA) microspheres was achieved via the help of UV irradiation, and the designed FPA microspheres were employed as Fenton-like catalysts to degrade micropollutants. Results showed that UV irradiation could activate the generation of the polydopamine shell and accelerate the Ag deposition, which played a crucial role in the rapid synthesis of highly active and stable FPA catalysts. Relative to reported catalysts, these FPA microspheres exhibited outstanding catalytic degradation performance, achieving 94.38% removal of tetracycline within 60 min. This work will provide a convenient strategy in the sustainable and efficient purification of wastewater to improve the quality of human life.
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Peróxido de Hidrogênio , Polímeros , Catálise , Humanos , IndóisRESUMO
Bacterial infections are a common problem associated with wound treatment that imposes a significant burden on healthcare systems and patients. As a result, healthcare providers urgently need new treatment strategies to protect people. Hydrogel biomaterials with inherent antimicrobial properties offer an attractive and viable solution to this issue. Here, for the first time, we have developed a new efficient synthetic strategy to prepare cationic hydrogels (PHCI) with intrinsically efficient antimicrobial properties by chemically cross-linking trans-1,4-cyclohexanediamine with 1,3-dibromo-2-propanol using a condensation reaction without the use of toxic cross-linking agents. As expected, the prepared PHCI hydrogel possessed an inherent antibacterial ability that can adsorb and kill Staphylococcus aureus and Escherichia coli electrostatically. Notably, in vivo experiments on normal and diabetic rat models confirmed that the PHCI hydrogel can quickly stop bleeding, efficiently kill bacteria, promote the conversion of macrophages from the proinflammatory M1 phenotype to the repaired M2 phenotype, and accelerate collagen deposition and blood vessel formation, thereby achieving rapid wound healing. Overall, this work presents an effective antibacterial dressing that might provide a facile but effective approach for clinical wound management.
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Antibacterianos/química , Antibacterianos/farmacologia , Infecções Bacterianas/tratamento farmacológico , Hidrogéis/química , Hidrogéis/farmacologia , Cicatrização/efeitos dos fármacos , Infecção dos Ferimentos/tratamento farmacológico , Animais , Antibacterianos/uso terapêutico , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Diabetes Mellitus Experimental/complicações , Escherichia coli/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Hemostáticos/química , Hemostáticos/farmacologia , Hemostáticos/uso terapêutico , Hidrogéis/uso terapêutico , Masculino , Camundongos , Ratos Sprague-Dawley , Staphylococcus aureus/efeitos dos fármacos , Infecção dos Ferimentos/complicações , Infecção dos Ferimentos/metabolismo , Infecção dos Ferimentos/patologiaRESUMO
Cervical carcinoma is a leading malignant tumor among women worldwide, characterized by the dysregulation of cell cycle. Cyclin-dependent kinase 6 (CDK6) plays important roles in the cell cycle progression, cell differentiation, and tumorigenesis. However, the role of CDK6 in cervical cancer remains controversial. Here, we found that loss of CDK6 in cervical adenocarcinoma HeLa cell line inhibited cell proliferation but induced apoptosis as well as autophagy, accompanied by attenuated expression of mammalian target of rapamycin complex 1 (mTORC1) and hexokinase 2 (HK2), reduced glycolysis, and production of protein, nucleotide, and lipid. Similarly, we showed that CDK6 knockout inhibited the survival of CDK6-high CaSki but not CDK6-low SiHa cervical cancer cells by regulation of glycolysis and autophagy process. Collectively, our studies indicate that CDK6 is a critical regulator of human cervical cancer cells, especially with high CDK6 level, through its ability to regulate cellular apoptosis and metabolism. Thus, inhibition of CDK6 kinase activity could be a powerful therapeutic avenue used to treat cervical cancers.
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Quinase 6 Dependente de Ciclina , Neoplasias do Colo do Útero , Apoptose , Autofagia , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Glicólise , Células HeLa , Hexoquinase/genética , Hexoquinase/metabolismo , Humanos , Masculino , Alvo Mecanístico do Complexo 1 de Rapamicina/metabolismo , Neoplasias do Colo do Útero/patologiaRESUMO
With the increasingly severe international security situation, the application of explosives is more and more extensive, and the probes that can detect the explosives quickly and efficiently have attracted people's attention. In this work, two novel probes T1 and T2 were synthesized through vitamin E succinate and tetraphenylethylene derivative. Fluorescence spectra showed that both T1 and T2 had a typical aggregation-induced emission (AIE) effect in THF/H2O solution, and explosive FOX-7 could effectively quench this fluorescence without being affected by other explosives or ions. The filter paper and cotton rods prepared with these two probes could detect FOX-7 specifically, which also provided the possibility for practical application on the battlefield.
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BACKGROUND/OBJECTIVES: The consumption of home meal replacement (HMR) is increasing among college students in Korea and China. In particular, Chinese college students studying abroad in Korea (CSK) show changes in their dietary behavior after migration, and HMR consumption for meal substitution is also increasing. This study was conducted to compare the HMR consumption behaviors and HMR development needs of CSK, Chinese college students in China (CSC), and Korean college students in Korea (KSK). SUBJECTS/METHODS: The subjects were 570 college students (180 CSK, 200 CSC, and 190 KSK) who had experience of HMR consumption. Data were collected by face-to-face survey in 2019 and analyzed using SPSS 25.0. RESULTS: The majority of the subjects purchased HMR to 'saving time' and 'preventing meal skipping'. Average purchase price per HMR was about 5,000 won for the CSK and KSK, and about 3,000 won for the CSC. The most important attributes when selecting HMR for the CSK and CSC were hygiene, freshness, and taste in that order, while for the KSK were taste, price, and hygiene. Rice was preferred by the KSK while grilled and fried dishes were preferred by the CSK and CSC. In terms of development needs, dessert and meat-based side dishes were highest in all three groups. The preferred food materials for more than 50% of the subjects of all groups were beef, chicken, pork, shrimp, and squid, and spinach and Chinese cabbage in the CSK, and onion in the KSK. CONCLUSIONS: This study shows more effort is needed to develop the healthy customized HMR for college students studying in Korea and China, and that focuses are placed by CSK on hygiene and freshness, by CSC on meat side dishes, hygiene, and price, and by KSK on snacks (as meal substitutes), taste, and price.