RESUMO
Stem cell research has been attained a greater attention in most fields of medicine due to its potential for many incurable diseases through replacing or helping the regeneration of damaged cells or tissues. Here, we demonstrated the functional recovery and structural connection of the central nervous system pathway innervating the sciatic nerve after total transection of the spinal cord followed by the transplantation of human neural stem cells (hNSC) in the injured rat spinal cord site. The limb function of hNSC-treated group recovered dramatically compared with that in the sham group by Basso-Beattie-Bresnahan (BBB) scores. Transplanted hNSC differentiated into astrocytes and neurons in the injured site. In addition, immunohistochemistry for growth-associated protein 43 showed axonal regeneration in the injured spinal cord site. The pseudorabies viral-Ba (PRV-Ba) tracing method revealed that transplanted hNSC and their differentiated neurons showed positive labeling after sciatic nerve injection. In addition, the PRV-Ba labeling was also observed in several nuclei in the brain innervating the sciatic nerve. This result implies that the rat CNS motor pathway could be reconstructed by hNSC transplantation, and it may contribute to the functional recovery of the limb.
Assuntos
Vias Neurais/fisiopatologia , Células-Tronco Neurais/transplante , Recuperação de Função Fisiológica , Nervo Isquiático/fisiologia , Traumatismos da Medula Espinal/reabilitação , Traumatismos da Medula Espinal/terapia , Animais , Células Cultivadas , Sistema Nervoso Central/fisiologia , Sistema Nervoso Central/fisiopatologia , Modelos Animais de Doenças , Vias Eferentes/fisiopatologia , Feminino , Humanos , Regeneração Nervosa/fisiologia , Vias Neurais/fisiologia , Ratos , Ratos Sprague-Dawley , Recuperação de Função Fisiológica/fisiologia , Nervo Isquiático/fisiopatologia , Traumatismos da Medula Espinal/patologia , Traumatismos da Medula Espinal/fisiopatologia , Transplante de Células-Tronco/métodos , Transplante HeterólogoRESUMO
Serial changes of bone density in 26 femora and 22 tibiae that underwent leg lengthening by distraction osteogenesis were investigated by measuring the pixel value shown on a picture archiving and communication systems (PACS) monitor. The pixel value of the original cortex did not change significantly until the external fixator was removed. The pixel value of the distraction sites did not change significantly for the first 5-6 weeks/cm, but rapidly increased after 7 weeks/cm in the femur and tibia, then slowly increased after 8 weeks/cm. The serial changes of the pixel value of the distracted bone showed a sigmoid curve, rather than a straight increasing line. The relative pixel values of the lengthening sites were >95% in three of the four cortices at external fixator removal. Thus, the pixel value of PACS is a rapid and easy method for the detection of bone density changes in distraction osteogenesis.
Assuntos
Densidade Óssea/fisiologia , Fêmur/diagnóstico por imagem , Desigualdade de Membros Inferiores/cirurgia , Osteogênese por Distração/métodos , Sistemas de Informação em Radiologia , Tíbia/diagnóstico por imagem , Absorciometria de Fóton/métodos , Adolescente , Adulto , Análise de Variância , Alongamento Ósseo , Criança , Estudos de Coortes , Feminino , Fêmur/cirurgia , Humanos , Desigualdade de Membros Inferiores/diagnóstico por imagem , Masculino , Monitorização Fisiológica/métodos , Probabilidade , Estudos Prospectivos , Sensibilidade e Especificidade , Tíbia/cirurgia , Resultado do TratamentoRESUMO
We studied the outcome of expansive laminoplasty in 37 patients with cervical spondylotic myelopathy. Patients were divided into two groups according to the recovery rate (RR) - a 'good' group ( n=19), and a 'fair' group ( n=18). Patients in the good group showed a greater pre-operative Japanese Orthopaedic Association (JOA) score, a greater compression ratio, and a larger Pavlov ratio ( P<0.05). The presence of high signal intensity on MRI proved to be of no prognostic importance.