RESUMO
OBJECTIVE: To evaluate the in vitro antibacterial and cytotoxic properties of the methanolic extract of Pelargonium peltatum (geranium) against Streptococcus mutans (ATCC 25175) and Streptococcus sanguinis (ATCC 10556). METHODS: Three extracts of P. peltatum were prepared using the leaf, stem, and root. Nine independent assays were prepared for each type of extract with chlorhexidine at 0.12% as the positive control. The agar diffusion method was performed to determine the antibacterial properties of each extract. The minimum inhibitory concentration (MIC) was determined using the microdilution method, and the cytotoxicity was analyzed by means of the MTT reduction test using a MDCK cell line. RESULTS: The root extract had the highest antibacterial effect with a mean result of (27.68 ± 0.97) mm and (30.80 ± 0.55) mm against S. mutans and S. sanguinis, respectively. The minimum inhibitory concentration for the leaf and root extracts was 250 mg/mL for S. mutans and 125 mg/mL for S. sanguinis. Cytotoxicity assays showed that both extracts had a low cytotoxicity at high concentrations. The cellular viability was highest for the root extract at 95.3% followed by the stem extract at 80.8% and finally the leaf extract with 75.4%. CONCLUSIONS: These findings show the antibacterial properties of the methanolic extracts of P. pelargonium against S. mutans and S. sanguinis. These extracts were not cytotoxic at high concentrations.
RESUMO
La ortodoncia osteogénica periodontalmente acelerada es un procedimiento indicado para minimizar el tiempo de tratamiento ortodóncico brindando una alternativa de tratamiento a pacientes periodontalmente comprometidos, con presencia de reabsorciones radiculares, periodonto reducido, entre otros. Los problemas periodontales no diagnosticados pueden ser agravados durante el tratamiento ortodóncico y dependiendo de su severidad, pueden requerir un tratamiento previo. El objetivo de este reporte es presentar el caso de una paciente con maloclusión Clase I con apinÌamiento y recesión gingival, tratada con injerto conectivo de tejido libre, corticotomía con injerto óseo y técnica de arco recto prescripción CCO. Finalmente, se puede concluir que el manejo interdisciplinario es importante para el correcto diagnóstico y tratamiento de los pacientes. La ortodoncia osteogénica periodontalmente acelerada es una alternativa viable que reduce el tiempo de tratamiento ortodóncico y mantiene o mejorá la condición periodontal de los pacientes. Palabras clave: Ortodoncia; Recesión gingival; Periodoncia (fuente: DeCS BIREME).
Periodontally accelerated osteogenic orthodontics is a procedure indicated to reduce the time of orthodontic treatment by giving an alternative treatment to periodontally compromised patients, with presence of root resorption, reduced periodontium, among others. Undiagnosed periodontal problems can be aggravated during orthodontic treatment and, depending on their severity, may require prior treatment. The objective of this report is to present the case of a patient with Class I malocclusion with crowding and gingival recession, treated with free tissue connective graft, corticotomy with bone graft and CCO Straighwire prescription technique. Finally, it can be concluded that interdisciplinary management is important for the correct diagnosis and treatment of patients. Periodontally accelerated osteogenic orthodontics is a viable alternative that reduces the time of orthodontic treatment and maintains or improves the periodontal condition of patients. Keywords: Orthodontic treatment; Gingival recession; Periodontics (source: MeSH NLM).