Genetic analysis of Giardia duodenalis isolates from children of low-income families living in an economically successful region in Southeastern Brazil.

Giardia duodenalis is one of the most important and widespread gastrointestinal parasites in the world. Despite its relevance as a causative agent of diarrhea, asymptomatic giardiasis occurs frequently, especially in low resources settings in which children are exposed to many risk factors. Based on microscopic examination and the polymerase chain reaction (PCR) amplification and sequencing of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes, we assessed G. duodenalis occurrence and genetic diversity in isolates of children attending a daycare center and living in low income families, in an economically successful region. Considering both, microscopic examination and PCR/sequencing methods, the overall prevalence of Giardia infection was 51.4%, with the highest frequency in children aged 1-4 years old (p<0.05). Genotyping of 50 isolates revealed that the assemblage A was found in 60% of the samples (30/50), followed by the assemblage B in 38% (19/50) and 2% of mixed-assemblage infections (1/50). At the sub-assemblage level, isolates genotyped as A were AII and among isolates B, BIII and BIV were identified. Both assemblages A and B were detected in children of all age groups, however assemblage A was more prevalent. The detection of anthroponotic assemblages and sub-assemblages (AII, BIII and BIV) reinforces human-to-human transmission, mainly in children of all age groups when they have not yet received toilet training, making them more vulnerable to infection.

Benefícios e desafios do uso de sistemas de informação na atuação do profissional de enfermagem / Beneficios y desafíos del uso de sistemas de información en el desempeño de los profesionales de enfermería / Applications, benefits and challenges os using information systems in the perfomance of nursing

Objetivo: Apresentar benefícios e entraves que impedem os profissionais de Enfermagem utilizarem sistemas de informação (SI). Métodos: Trata-se de revisão sistemática. Dados coletados nas bases: Scielo, Biblioteca Virtual em Saúde (BVS) e PubMed Centralcom descritores: enfermagem AND sistemas de informação AND aplicações OR beneficios OR limitações,utilizando a estratégia PICO.Critério de inclusão: artigos completos, idioma Português, período 2019 a 2021, excluídos artigos repetidos e não vinculados ao tema. Resultados:Analisadas sete publicações que discutem dificuldades na utilização do sistema devido ao atraso na inserção das informações do paciente,baixa adesão e aceitabilidade entre os profissionais. Pontos positivos encontrados foram otimização de tempo, redução de atividades burocráticas, agilidade para tomar decisões corretas no atendimento ao paciente.Conclusão: Os sistemas de informação fornecem o armazenamento, a organização e o controle das informações que facilitam o conhecimento prévio do paciente; além de fornecer o suporte necessário para a tomada de decisão na prática de Enfermagem(AU)

Objective: To present benefits and obstacles that prevent Nursing professionals from using information systems (IS). Methods: This is a systematic review. Data collected in the following databases: Scielo, Virtual Health Library (BVS) and PubMed Central with descriptors: nursing AND information systemsAND applications OR benefits OR limitations, using the PICO strategy. Inclusion criteria: full articles, Portuguese language, period 2019 to 2021, excluding repeated articles not linked to the theme. Results: Seven publications were analyzed that discuss difficulties in using the system due to the delay in entering patient information, low adherence and acceptability among professionals. Positive points found were time optimization, reduction of bureaucratic activities, agility to make correct decisions in patient care. Conclusion: Information systems provide the storage, organization and control of information that facilitate prior knowledge of the patient; in addition to providing the necessary support for decision-making in Nursing practice.(AU)

Objetivo: Presentar beneficios y obstáculos que impiden que los profesionales de Enfermería utilicen los sistemas de información (SI). Métodos: Esta es una revisión sistemática. Datos recolectados en las siguientes bases de datos: Scielo, Biblioteca Virtual en Salud (BVS) y PubMed Central con descriptores: enfermería Y sistemas de información Y aplicaciones O beneficios O limitaciones, utilizando la estrategia PICO. Criterios de inclusión: artículos completos, idioma portugués, período 2019 a 2021, excluyendo artículos repetidos no vinculados al tema. Resultados: Se analizaron siete publicaciones que discuten las dificultades en el uso del sistema debido a la demora en el ingreso de la información del paciente, baja adherencia y aceptabilidad entre los profesionales. Los puntos positivos encontrados fueron optimización de tiempo, reducción de actividades burocráticas, agilidad para tomar decisiones correctas en la atención al paciente Conclusión: Los sistemas de información brindan el almacenamiento, organización y control de la información que facilitan el conocimiento previo del paciente; además de brindar el apoyo necesario para la toma de decisiones en la práctica de Enfermería.(AU)