RESUMO
High density lipoproteins (HDL) are small plasma particles which may be able to pass through the blood-brain barrier. We have therefore studied the fatty acids of HDL in patients with dementia to determine whether the changes are consistent with those previously reported in brain tissue. The HDL phospholipid and the HDL cholesteryl ester both showed reduced concentrations of arachidonic acid (20:4n6) as compared to normal controls. HDL may be a useful plasma fraction for study of lipids in neurodegenerative diseases.
Assuntos
Envelhecimento/metabolismo , Demência/metabolismo , Ácidos Graxos/análise , Lipoproteínas HDL/química , Idoso , Idoso de 80 Anos ou mais , Envelhecimento/patologia , Doença de Alzheimer/metabolismo , Doença de Alzheimer/psicologia , Ácido Araquidônico/metabolismo , Interpretação Estatística de Dados , Demência/psicologia , Ácido Eicosapentaenoico/metabolismo , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Ácidos Graxos Insaturados/análise , Ácidos Graxos Insaturados/química , Ácidos Graxos Insaturados/metabolismo , Feminino , Humanos , Lipoproteínas HDL/metabolismo , Masculino , Pessoa de Meia-Idade , Testes Neuropsicológicos , Fosfolipídeos/química , Fosfolipídeos/metabolismo , Estatísticas não Paramétricas , Triglicerídeos/química , Triglicerídeos/metabolismoRESUMO
Antisera were raised to whole, live cells of a reference strain (NCTC 9344) and two clinical isolates (GNAB 92 and GNAB 4) of Bacteroides fragilis. Each antiserum was reacted in crossed line immunoelectrophoresis (CLIE) with EDTA-heat-sonication-prepared outer membrane (OM) complex from 10 B. fragilis strains. In addition, the antisera were reacted with these antigens in an enzyme-linked immunosorbent assay (ELISA). In CLIE, the antisera raised to the reference strain and one of the clinical isolates (GNAB 92) demonstrated a heat labile antigen which was common to all 10 of the test strains. Lipopolysaccharide (LPS) prepared from both the clinical isolates produced three major precipitin lines when reacted with their homologous antisera in crossed immunoelectrophoresis (CIE). In both cases, these three antigens were present as major components of the OM complex. Each antiserum reacted significantly in ELISA with all test OM complex preparations. Inhibition of ELISA showed that carbohydrates were the predominant cross-reactive antigens in ELISA and that in the case of the clinical isolate GNAB 4, most of the cross-reactive antigenic activity was present in the homologous LPS preparation.